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WO2001072783A3 - Production accrue de proteines secretees par des cellules eucaryotes recombinantes - Google Patents

Production accrue de proteines secretees par des cellules eucaryotes recombinantes Download PDF

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Publication number
WO2001072783A3
WO2001072783A3 PCT/US2001/009401 US0109401W WO0172783A3 WO 2001072783 A3 WO2001072783 A3 WO 2001072783A3 US 0109401 W US0109401 W US 0109401W WO 0172783 A3 WO0172783 A3 WO 0172783A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
secreted
production
eukaryotic cells
secreted proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/009401
Other languages
English (en)
Other versions
WO2001072783A2 (fr
Inventor
Merja E Penttila
Michael Ward
Huaming Wang
Mari J Valkonen
Markku L A Saloheimo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Priority to DK01924287T priority Critical patent/DK1266018T3/da
Priority to DE60133884T priority patent/DE60133884D1/de
Priority to AU2001250954A priority patent/AU2001250954A1/en
Priority to EP01924287A priority patent/EP1266018B1/fr
Priority to CA2403842A priority patent/CA2403842C/fr
Publication of WO2001072783A2 publication Critical patent/WO2001072783A2/fr
Publication of WO2001072783A3 publication Critical patent/WO2001072783A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/11—Protein-serine/threonine kinases (2.7.11)
    • C12Y207/11001—Non-specific serine/threonine protein kinase (2.7.11.1), i.e. casein kinase or checkpoint kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03016—Phosphoprotein phosphatase (3.1.3.16), i.e. calcineurin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes destinées à augmenter la quantité de protéines sécrétées par une cellule. Dans un mode de réalisation, une cellule contient un acide nucléique hétérologue codant pour une protéine présentant une activité modulant la réponse d'une protéine dépliée, ainsi qu'un acide nucléique hétérologue codant pour une protéine d'intérêt à sécréter. Dans un autre mode de réalisation, la protéine dont l'activité module la réponse d'une protéine dépliée est choisie parmi les protéines appartenant au groupe HAC1, PTC2 et IRE1. La protéine d'intérêt peut être une protéine sécrétée quelconque, telle qu'une enzyme industrielle ou thérapeutique. Par exemple, cette protéine peut être choisie dans le groupe constitué par une lipase, une cellulase, une endoglucosidase H, une protéase, une carbohydrase, une réductase, une oxydase, une isomérase, une transférase, une kinase, une phosphatase, une alpha-amylase, une glucamylase, une hémicellulase de lignocellulose, une pectinase et une ligninase.
PCT/US2001/009401 2000-03-24 2001-03-23 Production accrue de proteines secretees par des cellules eucaryotes recombinantes Ceased WO2001072783A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DK01924287T DK1266018T3 (da) 2000-03-24 2001-03-23 Fremstilling af udskilte proteiner af rekombinante, eukaryotiske celler
DE60133884T DE60133884D1 (de) 2000-03-24 2001-03-23 Herstellung von sekretierten proteinen durch rekombinante eukaryotische zellen
AU2001250954A AU2001250954A1 (en) 2000-03-24 2001-03-23 Increased production of secreted proteins by recombinant eukaryotic cells
EP01924287A EP1266018B1 (fr) 2000-03-24 2001-03-23 Production accrue de proteines secretees par des cellules eucaryotes recombinantes
CA2403842A CA2403842C (fr) 2000-03-24 2001-03-23 Production accrue de proteines secretees par des cellules eucaryotes recombinantes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53469200A 2000-03-24 2000-03-24
US09/534,692 2000-03-24

Publications (2)

Publication Number Publication Date
WO2001072783A2 WO2001072783A2 (fr) 2001-10-04
WO2001072783A3 true WO2001072783A3 (fr) 2002-07-18

Family

ID=24131126

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/009401 Ceased WO2001072783A2 (fr) 2000-03-24 2001-03-23 Production accrue de proteines secretees par des cellules eucaryotes recombinantes

Country Status (8)

Country Link
US (4) US20010034045A1 (fr)
EP (1) EP1266018B1 (fr)
AT (1) ATE394496T1 (fr)
AU (1) AU2001250954A1 (fr)
CA (1) CA2403842C (fr)
DE (1) DE60133884D1 (fr)
DK (1) DK1266018T3 (fr)
WO (1) WO2001072783A2 (fr)

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Also Published As

Publication number Publication date
US20140335591A1 (en) 2014-11-13
EP1266018A2 (fr) 2002-12-18
CA2403842C (fr) 2012-09-25
EP1266018B1 (fr) 2008-05-07
US20100221775A1 (en) 2010-09-02
AU2001250954A1 (en) 2001-10-08
US20010034045A1 (en) 2001-10-25
CA2403842A1 (fr) 2001-10-04
DE60133884D1 (de) 2008-06-19
ATE394496T1 (de) 2008-05-15
DK1266018T3 (da) 2008-09-01
US7662584B2 (en) 2010-02-16
US8753866B2 (en) 2014-06-17
US20040186070A1 (en) 2004-09-23
WO2001072783A2 (fr) 2001-10-04

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