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WO2001053532A3 - Barcoded synthetic lethal screening to identify drug targets - Google Patents

Barcoded synthetic lethal screening to identify drug targets Download PDF

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Publication number
WO2001053532A3
WO2001053532A3 PCT/US2001/001661 US0101661W WO0153532A3 WO 2001053532 A3 WO2001053532 A3 WO 2001053532A3 US 0101661 W US0101661 W US 0101661W WO 0153532 A3 WO0153532 A3 WO 0153532A3
Authority
WO
WIPO (PCT)
Prior art keywords
synthetic lethal
drug targets
identify drug
clone
barcoded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/001661
Other languages
French (fr)
Other versions
WO2001053532A2 (en
Inventor
Charles M Brenner
Daniel D Shoemaker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thomas Jefferson University
Rosetta Inpharmatics LLC
Original Assignee
Thomas Jefferson University
Rosetta Inpharmatics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thomas Jefferson University, Rosetta Inpharmatics LLC filed Critical Thomas Jefferson University
Priority to JP2001553391A priority Critical patent/JP2003535576A/en
Priority to AU2001229589A priority patent/AU2001229589A1/en
Priority to US10/182,209 priority patent/US20040121324A1/en
Priority to CA002398690A priority patent/CA2398690A1/en
Priority to EP01942675A priority patent/EP1248860A2/en
Publication of WO2001053532A2 publication Critical patent/WO2001053532A2/en
Publication of WO2001053532A3 publication Critical patent/WO2001053532A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to methods of using synthetic lethal screening techniques to identify drug targets. The methods of the present invention use 'barcoded' libraries of cells, where the library consists of a collection of different mutant clones, each mutant clone bearing a knock-out mutation of a different gene. Each mutant clone has a unique DNA identifier tag, or 'barcode', to allow for quick and convenient identification of the clone and its mutation. The use of such a library allows for rapid, quantitative, sensitive and simple identification of genes which interact with a mutated target gene. So identified genes are promising targets for drug screening.
PCT/US2001/001661 2000-01-20 2001-01-18 Barcoded synthetic lethal screening to identify drug targets Ceased WO2001053532A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2001553391A JP2003535576A (en) 2000-01-20 2001-01-18 Bar-coded synthetic lethal screening to identify drug targets
AU2001229589A AU2001229589A1 (en) 2000-01-20 2001-01-18 Barcoded synthetic lethal screening to identify drug targets
US10/182,209 US20040121324A1 (en) 2001-01-18 2001-01-18 Barcoded synthetic lethal screening to identify drug targets
CA002398690A CA2398690A1 (en) 2000-01-20 2001-01-18 Barcoded synthetic lethal screening to identify drug targets
EP01942675A EP1248860A2 (en) 2000-01-20 2001-01-18 Barcoded synthetic lethal screening to identify drug targets

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11746000P 2000-01-20 2000-01-20
US60/117,460 2000-01-20

Publications (2)

Publication Number Publication Date
WO2001053532A2 WO2001053532A2 (en) 2001-07-26
WO2001053532A3 true WO2001053532A3 (en) 2002-02-21

Family

ID=22373061

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/001661 Ceased WO2001053532A2 (en) 2000-01-20 2001-01-18 Barcoded synthetic lethal screening to identify drug targets

Country Status (5)

Country Link
EP (1) EP1248860A2 (en)
JP (1) JP2003535576A (en)
AU (1) AU2001229589A1 (en)
CA (1) CA2398690A1 (en)
WO (1) WO2001053532A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2513541A1 (en) 2003-01-29 2004-08-19 454 Corporation Method for preparing single-stranded dna libraries
AU2014310695B2 (en) 2013-08-21 2018-03-15 Mycartis Nv Heterogenous surface functionalization
WO2022133734A1 (en) * 2020-12-22 2022-06-30 Singleron (Nanjing) Biotechnologies, Ltd. Methods and reagents for high-throughput transcriptome sequencing for drug screening
WO2023102610A1 (en) * 2021-12-08 2023-06-15 The University Of Queensland Methods and compositions for multiplexing cell analysis

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999024603A1 (en) * 1997-11-06 1999-05-20 Fred Hutchinson Cancer Research Center Method for identifying drug targets

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999024603A1 (en) * 1997-11-06 1999-05-20 Fred Hutchinson Cancer Research Center Method for identifying drug targets

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BOISRAME A ET AL: "A mutation in the secretion pathway of the yeast Yarrowia lipolytica that displays synthetic lethality in combination with a mutation affecting the signal recognition particle.", MOLECULAR AND GENERAL GENETICS, vol. 261, no. 4-5, June 1999 (1999-06-01), pages 601 - 609, XP002179867, ISSN: 0026-8925 *
SHOEMAKER D D ET AL: "QUANTITATIVE PHENOTYPIC ANALYSIS OF YEAST DELETION MUTANTS USING A HIGHLY PARALLEL MOLECULAR BAR-CODING STRATEGY", NATURE GENETICS, NEW YORK, NY, US, vol. 14, no. 4, 1 December 1996 (1996-12-01), pages 450 - 456, XP002043431, ISSN: 1061-4036 *
WINZELER ELIZABETH A ET AL: "Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.", SCIENCE (WASHINGTON D C), vol. 285, no. 5429, 6 August 1999 (1999-08-06), pages 901 - 906, XP001025980, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
WO2001053532A2 (en) 2001-07-26
AU2001229589A1 (en) 2001-07-31
CA2398690A1 (en) 2001-07-26
JP2003535576A (en) 2003-12-02
EP1248860A2 (en) 2002-10-16

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