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WO2000029621A3 - Method for measuring target polynucleotides and novel asthma biomolecules - Google Patents

Method for measuring target polynucleotides and novel asthma biomolecules Download PDF

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Publication number
WO2000029621A3
WO2000029621A3 PCT/US1999/026931 US9926931W WO0029621A3 WO 2000029621 A3 WO2000029621 A3 WO 2000029621A3 US 9926931 W US9926931 W US 9926931W WO 0029621 A3 WO0029621 A3 WO 0029621A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
target
conjugate
dimers
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/026931
Other languages
French (fr)
Other versions
WO2000029621A2 (en
Inventor
Gregory Dolganov
Alexander Novikov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genelabs Technologies Inc
Original Assignee
Genelabs Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genelabs Technologies Inc filed Critical Genelabs Technologies Inc
Priority to AU14782/00A priority Critical patent/AU1478200A/en
Publication of WO2000029621A2 publication Critical patent/WO2000029621A2/en
Publication of WO2000029621A3 publication Critical patent/WO2000029621A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/521—Chemokines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides a method for simultaneously determining the levels of selected target polynucleotide sequences in a sample. In the method, selected target sequences are amplified using sequence-selective primer pairs to form double-stranded copies. The copies are cleaved with one or more endonucleases, and first and second aliquots of the cleaved fragment mixture are ligated to first and second adaptors, respectively, to form mixtures of first and second adaptor-fragment conjugates. The aliquots are combined and ligated to form conjugate dimers containing a conjugate from each of the first and second conjugate mixtures. After optional amplification, the conjugates are treated to release the adaptor segments, yielding target-identifier dimers. The dimers are polymerized to form target-identifier dimer multimers, and the relative abundances of the target-identifiers in one or more dimer multimers to provide an estimate of the levels of the selected target sequences in the sample. The present invention is particularly useful for determining expression levels of mRNA gene sequences in a sample, and for evaluating changes in mRNA expression levels among different samples or in response to changes in sample conditions. Also disclosed are certain polynucleotides and polypeptides that are useful for a variety of applications, particularly relating to asthma.
PCT/US1999/026931 1998-11-16 1999-11-12 Method for measuring target polynucleotides and novel asthma biomolecules Ceased WO2000029621A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU14782/00A AU1478200A (en) 1998-11-16 1999-11-12 Method for measuring target polynucleotides and novel asthma biomolecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19332098A 1998-11-16 1998-11-16
US09/193,320 1998-11-16

Publications (2)

Publication Number Publication Date
WO2000029621A2 WO2000029621A2 (en) 2000-05-25
WO2000029621A3 true WO2000029621A3 (en) 2000-11-09

Family

ID=22713144

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/026931 Ceased WO2000029621A2 (en) 1998-11-16 1999-11-12 Method for measuring target polynucleotides and novel asthma biomolecules

Country Status (2)

Country Link
AU (1) AU1478200A (en)
WO (1) WO2000029621A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1620089A4 (en) * 2003-03-26 2008-12-24 Crc For Asthma Ltd Therapeutic and prophylactic compositions and uses therefor

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018176A1 (en) * 1992-03-11 1993-09-16 Dana-Farber Cancer Institute METHODS TO CLONE mRNA
WO1994023023A1 (en) * 1993-04-06 1994-10-13 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
WO1996034985A1 (en) * 1995-05-05 1996-11-07 Sugen, Inc. Assay and method for transcript imaging
EP0743367A2 (en) * 1995-05-19 1996-11-20 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Berlin Gene expression analysis
WO1997010363A1 (en) * 1995-09-12 1997-03-20 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
WO1997022720A1 (en) * 1995-12-21 1997-06-26 Kenneth Loren Beattie Arbitrary sequence oligonucleotide fingerprinting
WO1997027284A2 (en) * 1996-01-26 1997-07-31 Genelabs Technologies, Inc. Human rad50 and septin-2 genes and methods of use
WO1998010095A1 (en) * 1996-09-05 1998-03-12 Brax Genomics Limited Characterising dna
WO1998037191A1 (en) * 1997-02-20 1998-08-27 Forssmann Wolf Georg Human circulating hnlp (human neutrophil lymphocyte peptide)

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018176A1 (en) * 1992-03-11 1993-09-16 Dana-Farber Cancer Institute METHODS TO CLONE mRNA
WO1994023023A1 (en) * 1993-04-06 1994-10-13 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
WO1996034985A1 (en) * 1995-05-05 1996-11-07 Sugen, Inc. Assay and method for transcript imaging
EP0743367A2 (en) * 1995-05-19 1996-11-20 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Berlin Gene expression analysis
WO1997010363A1 (en) * 1995-09-12 1997-03-20 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
WO1997022720A1 (en) * 1995-12-21 1997-06-26 Kenneth Loren Beattie Arbitrary sequence oligonucleotide fingerprinting
WO1997027284A2 (en) * 1996-01-26 1997-07-31 Genelabs Technologies, Inc. Human rad50 and septin-2 genes and methods of use
WO1998010095A1 (en) * 1996-09-05 1998-03-12 Brax Genomics Limited Characterising dna
WO1998037191A1 (en) * 1997-02-20 1998-08-27 Forssmann Wolf Georg Human circulating hnlp (human neutrophil lymphocyte peptide)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
COUSINS D J ET AL: "Regulation of cytokine genes implicated in asthma and atopy", GENETICS OF ASTHMA AND ATOPY, vol. 33, November 1996 (1996-11-01), pages 138 - 152, XP002101568 *
DATABASE EMBL 6 April 1998 (1998-04-06), KIMMERLY ET AL.: "HOMO SAPIENS CHROMOSOME 5, P1 CLONE 1076B9", XP002141569 *
STONE B AND WHARTON W: "Targeted RNA fingerprinting: The cloning of differentially-expressed cDNA fragments enriched for members of the zinc finger gene family", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 22, no. 13, 1 January 1994 (1994-01-01), pages 2612 - 2618, XP002090069, ISSN: 0305-1048 *
VELCULESCU V E ET AL: "SERIAL ANALYSIS OF GENE EXPRESSION", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 270, 20 October 1995 (1995-10-20), pages 484 - 487, XP002053721, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
AU1478200A (en) 2000-06-05
WO2000029621A2 (en) 2000-05-25

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