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WO2000024924A3 - Techniques de criblage de variantes de proteases servant dans des compositions detergentes - Google Patents

Techniques de criblage de variantes de proteases servant dans des compositions detergentes Download PDF

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Publication number
WO2000024924A3
WO2000024924A3 PCT/US1999/024822 US9924822W WO0024924A3 WO 2000024924 A3 WO2000024924 A3 WO 2000024924A3 US 9924822 W US9924822 W US 9924822W WO 0024924 A3 WO0024924 A3 WO 0024924A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
protease variants
detergent compositions
screening
screening protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/024822
Other languages
English (en)
Other versions
WO2000024924A2 (fr
Inventor
Chanchal Kumar Ghosh
Phillip Frederick Brode Iii
Deborah Susan Rauch
Michael Stanford Showell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Procter and Gamble Co
Original Assignee
Procter and Gamble Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter and Gamble Co filed Critical Procter and Gamble Co
Priority to EP99971044A priority Critical patent/EP1124987A2/fr
Priority to AU12230/00A priority patent/AU1223000A/en
Priority to JP2000578476A priority patent/JP2003512012A/ja
Publication of WO2000024924A2 publication Critical patent/WO2000024924A2/fr
Publication of WO2000024924A3 publication Critical patent/WO2000024924A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des techniques de criblage de variantes de protéases servant dans des systèmes détergents. Ces techniques se rapportent à la charge nette des substitutions des variantes de protéases et aux propriétés d'adsorption desdites variantes. L'invention traite également de compositions détergentes comprenant des variantes de protéases criblées par de telles techniques.
PCT/US1999/024822 1998-10-23 1999-10-22 Techniques de criblage de variantes de proteases servant dans des compositions detergentes Ceased WO2000024924A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99971044A EP1124987A2 (fr) 1998-10-23 1999-10-22 Techniques de criblage de variantes de proteases servant dans des compositions detergentes
AU12230/00A AU1223000A (en) 1998-10-23 1999-10-22 Methods for screening protease variants for use in detergent compositions
JP2000578476A JP2003512012A (ja) 1998-10-23 1999-10-22 洗剤組成物に用いられるプロテアーゼ変異体のスクリーニング法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10542098P 1998-10-23 1998-10-23
US60/105,420 1998-10-23

Publications (2)

Publication Number Publication Date
WO2000024924A2 WO2000024924A2 (fr) 2000-05-04
WO2000024924A3 true WO2000024924A3 (fr) 2000-10-26

Family

ID=22305753

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/024822 Ceased WO2000024924A2 (fr) 1998-10-23 1999-10-22 Techniques de criblage de variantes de proteases servant dans des compositions detergentes

Country Status (5)

Country Link
EP (1) EP1124987A2 (fr)
JP (1) JP2003512012A (fr)
AR (1) AR020951A1 (fr)
AU (1) AU1223000A (fr)
WO (1) WO2000024924A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
BRPI0306955B1 (pt) * 2002-01-16 2016-03-22 Danisco Us Inc variante de protease subtilisina de bacillus lentus, dna, vetor de expressão, célula hospedeira procariótica, bem como composição de limpeza
US7888093B2 (en) 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
TWI319007B (en) * 2002-11-06 2010-01-01 Novozymes As Subtilase variants
CN101784662B (zh) * 2007-06-06 2014-11-05 丹尼斯科美国公司 用于改善蛋白质特性的方法
WO2010056671A1 (fr) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions et procédés comprenant un variant de subtilisine
CN102933708A (zh) 2010-05-06 2013-02-13 丹尼斯科美国公司 包含枯草杆菌蛋白酶变体的组合物和方法
US9856466B2 (en) * 2011-05-05 2018-01-02 Danisco Us Inc. Compositions and methods comprising serine protease variants
US20130005048A1 (en) * 2011-06-29 2013-01-03 Monika Felten Indicator device
CN105349569B (zh) * 2015-11-19 2020-05-12 湖北大学 一组不同启动子介导的蛋白酶高通量筛选载体及筛选方法
EP4525615A2 (fr) 2022-05-14 2025-03-26 Novozymes A/S Compositions et procédés de prévention, de traitement, de suppression et/ou d'élimination d'infestations et d'infections phytopathogènes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991000345A1 (fr) * 1989-06-26 1991-01-10 Novo Nordisk A/S Protease de subtilisine ayant subi une mutation
WO1994002618A1 (fr) * 1992-07-17 1994-02-03 Gist-Brocades N.V. Serine-proteases hautement alcalines
WO1995023221A1 (fr) * 1994-02-24 1995-08-31 Cognis, Inc. Enzymes ameliorees et detergents les contenant
WO1999020726A1 (fr) * 1997-10-23 1999-04-29 The Procter & Gamble Company Compositions de blanchiment comprenant des variantes de protease multisubstituees

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991000345A1 (fr) * 1989-06-26 1991-01-10 Novo Nordisk A/S Protease de subtilisine ayant subi une mutation
WO1994002618A1 (fr) * 1992-07-17 1994-02-03 Gist-Brocades N.V. Serine-proteases hautement alcalines
WO1995023221A1 (fr) * 1994-02-24 1995-08-31 Cognis, Inc. Enzymes ameliorees et detergents les contenant
WO1999020726A1 (fr) * 1997-10-23 1999-04-29 The Procter & Gamble Company Compositions de blanchiment comprenant des variantes de protease multisubstituees
WO1999020771A2 (fr) * 1997-10-23 1999-04-29 Genencor International, Inc. Variantes de proteases a substitutions multiples et charge nette modifiee, utiles dans des detergents
WO1999020770A2 (fr) * 1997-10-23 1999-04-29 Genencor International, Inc. Variantes de proteases a substitutions multiples

Also Published As

Publication number Publication date
AU1223000A (en) 2000-05-15
JP2003512012A (ja) 2003-04-02
EP1124987A2 (fr) 2001-08-22
WO2000024924A2 (fr) 2000-05-04
AR020951A1 (es) 2002-06-05

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