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WO2000022406A2 - Procede et dispositif pour l'analyse de bandelettes reactives - Google Patents

Procede et dispositif pour l'analyse de bandelettes reactives Download PDF

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Publication number
WO2000022406A2
WO2000022406A2 PCT/DE1999/003272 DE9903272W WO0022406A2 WO 2000022406 A2 WO2000022406 A2 WO 2000022406A2 DE 9903272 W DE9903272 W DE 9903272W WO 0022406 A2 WO0022406 A2 WO 0022406A2
Authority
WO
WIPO (PCT)
Prior art keywords
reaction
optical sensor
strips
unit
cavities
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE1999/003272
Other languages
German (de)
English (en)
Other versions
WO2000022406A3 (fr
Inventor
Ina Matallana-Kielmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19933168A external-priority patent/DE19933168A1/de
Application filed by Individual filed Critical Individual
Priority to EP99959200A priority Critical patent/EP1119759A2/fr
Publication of WO2000022406A2 publication Critical patent/WO2000022406A2/fr
Publication of WO2000022406A3 publication Critical patent/WO2000022406A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band

Definitions

  • reaction strips are understood to mean carrier materials which are colored differently by chemical, biological or physical reactions.
  • these substrates are currently strip-shaped and the length of the strip is used to create differently colored areas. These areas, which are discolored relative to the stripe, are called bands.
  • the invention is also applicable to differently shaped carrier materials, such as rectangular or circular reaction plates.
  • reaction strips A main area of application for such reaction strips is in the detection of chromatographically or electrophoretically colored strips. These strips are referred to as blots, in particular as Western blots or dot blots, if antibody reactions on the strip are indicated by different bands.
  • the reaction strips are placed in a tub for development and exposed to liquid reagents there.
  • the invention has for its object to develop a faster method that largely excludes manual intervention.
  • reaction strips are placed in a receiving unit, in particular a trough, for development and are exposed there to liquid reagents, the surfaces of the reaction strips being detected by an optical sensor and the recorded data by an evaluation unit be forwarded.
  • the described method can be fully automated and reduces the manual handling of the reaction strips. This reduces the risk of the reaction strips being adversely affected by contact or contamination and enables the reaction strips to be processed more quickly.
  • the reaction strips preferably remain in the evaluation unit during the entire process.
  • the evaluation unit is trough-shaped for this purpose, so that the reaction strips in the evaluation unit can be soaked in liquid. If the liquid is only sprayed on, a flatter design of the evaluation unit is also possible.
  • the evaluation unit is designed so that it allows both the treatment of reaction strips with liquid reagents, as well as the detection of the surface of the reaction strips with an optical sensor.
  • reaction strips are irradiated with diffuse light. Irradiation with diffuse light in particular prevents evaluable reflections that can be attributed to the area surrounding the reaction strip and can lead to incorrect results.
  • a receiving unit with several cavities is used.
  • the cavity makes it easier to place a strip in the correct position on the take-up unit, and a thicker cavity even allows liquid reagents to be taken up.
  • the receiving unit has several cavities, several reaction strips can be used be inserted one after the other. In the method according to the invention, the strips are then successively exposed to liquid reagents and fed to the optical sensor in succession.
  • the described design of the pick-up unit thus enables a semi-automatic method in which the pick-up unit is first filled with several reaction strips, then these reaction strips are positioned one after the other under the optical sensor and finally the selected reaction strips are removed again from the pick-up unit.
  • the cavities are moved in a clocked manner relative to the optical sensor. This allows exact adherence to the reaction and the workflow of prescribed times.
  • Adherence to reaction times is also facilitated in that the reagents are filled into the cavity at defined positions relative to the optical sensor. If the cavities are moved at a constant speed, preferably clocked, relative to the optical sensor, the same period of time will therefore always lie between the filling and the optical sensor.
  • the object on which the invention is based is also achieved with a device for analyzing reaction strips which have a
  • Inclusion unit in particular a tub, an optical sensor and an evaluation unit.
  • This device enables automatic analysis of reaction strips and is particularly suitable for the method described above.
  • the recording unit and the strips are colored differently.
  • the different coloring makes it possible to eliminate the areas of the recording unit during the analysis and to examine only the areas which have the color of the reaction strip.
  • the receiving unit has several cavities. This allows the strips to be processed quickly.
  • a particularly advantageous embodiment of the device provides that the cavities are arranged on a circular line.
  • the cavities can either be arranged in a star shape on one level.
  • the reaction strips are inserted into radial cavities and the entire receiving unit is preferably rotated in a clocked manner around the center of the circular disk.
  • An alternative embodiment provides that the cavities are arranged on a conveyor belt rotating around two horizontal deflection rollers. While the cavities on the top of the conveyor belt are filled and the reaction strips are detected with the optical sensor, the reaction strips fall out of the cavity in the region of the deflection roller. On the underside of the belt, the cavities are conveyed back to the filling location with their opening facing downwards. In this area too a cleaning take place. The cavities then pass the second deflection roller and as soon as they are in a horizontal position again, they can be filled with reaction strips again.
  • the cavities have the shape of a reaction strip. This also allows the reaction strips to be positioned securely within the cavity and thus relative to the optical sensor.
  • the cavities be labeled differently with the optical sensor. This allows a specific cavity to be assigned to each recorded data record.
  • the pick-up unit To position the pick-up unit relative to the optical sensor, it is proposed that the pick-up unit have at least one marking. This marking makes it possible to move the pick-up unit in a motorized manner relative to the optical sensor and to switch off the drive of the movement mechanism when a special position of the marking is reached.
  • reaction strips have different labels that can be detected with the optical sensor.
  • the reaction strips can be provided with numbers so that everyone can evaluate the reaction strips Reaction strip data record is assigned a number that can also be found on the reaction strip.
  • a lighting unit which brings diffuse light to the tub.
  • the lighting unit is therefore designed so that the individual illuminants do not cause point-like reflections.
  • the lighting unit has illuminants and a light guide.
  • the light guide can distribute the light from the illuminant over a larger area to avoid selective reflections.
  • the light guide is preferably designed like a frosted glass, so that the light is refracted in the light guide.
  • the illuminants be light-emitting diodes, which preferably emit white light.
  • Several light-emitting diodes enable the light to be distributed evenly over a larger area or distance and thus enable the strip to be illuminated evenly.
  • the lighting unit be arranged in the immediate vicinity of the tub.
  • Light exit end arranged in close proximity to the tub while the lamps can also be arranged further away from the tub.
  • the lighting unit and the receiving unit are preferably matched to one another in such a way that as little light as possible reaches the strip between the lighting unit and the receiving unit.
  • a simple construction of the device is achieved in that a mirror optic is arranged between the reaction strip and the optical sensor. This allows the optical sensor and preferably a camera to be arranged at any position relative to the strip, since the image of the surface of the strip is projected onto the camera by means of one or more mirrors.
  • FIG. 1 shows a section through the device along the line I-I
  • Figure 2 shows a section through the device along the line II-II and
  • Figure 3 is a plan view of a receiving unit with a
  • the device 1 shown in the figures essentially consists of the trough 2 serving as the receiving unit, an optical sensor 3 and an evaluation unit 4.
  • the trough 2 has a cavity 5 in which a reaction strip 6 is located.
  • further cavities 7 and 8 adjoin on the right and left, in which reaction strips can also lie.
  • the optical sensor 3 is arranged above the trough 2.
  • This optical sensor 3 has on its underside 2 lighting devices 9 and 10 which radiate light downwards into the cavity 5 of the trough 2.
  • the lighting units 9 and 10 have light-emitting diodes 11 and 12 as illuminants, each of which is embedded in a frosted glass element 13, 14.
  • the milk glass element 13, 14 has approximately the length of the reaction strip 6 and is equipped with spaced-apart light-emitting diodes 12 over its entire length. This results in a uniform light distribution in the frosted glass element and, in particular, in a uniform light failure on the underside of the frosted glass element.
  • the milk glass element is preferably made of a transparent plastic and the light-emitting diodes emit white light which emerges as diffuse light on the underside of the milk glass element 13, 14.
  • the milk glass element 13, 14 is surrounded in a U-shape with a reflective film 15, 16, which only allows downward light loss.
  • Z between the lower exit areas of the frosted glass elements 13, 14, the reaction strip 6 is arranged and above this reaction strip 6 there is an oblique mirror 17 which projects an image of the reaction strip 6 onto the lens 18 of a camera 19.
  • the entire device 1 is light-tightly surrounded by a hood (not shown), so that light only enters the cavity 5 via the frosted glass elements 13 and 14. This light illuminates the reaction strip 6, so that a uniformly illuminated image of the reaction strip 6 is projected via the mirror 17 to the camera 19.
  • the camera 19 records the image and passes it on to the evaluation unit 4.
  • the cavities 5, 7, 8 are filled with liquid reagents and the reaction strips are inserted.
  • the optical sensor 3 is positioned over the cavity 5 and an image of the strip 6 is recorded with the camera 19. This image shows the different discolorations of the reaction strip 6, which are then analyzed with the evaluation unit 4.
  • the optical sensor 3 After analyzing a strip, the optical sensor 3 is moved further by one cavity relative to the trough 2 and the next reaction strip is examined.
  • the cavities 5, 7, 8 are shown in the plan view of the receiving unit 2 shown in FIG. 3, wherein the receiving trough 2 can have any number of cavities.
  • the reaction strip 6 lies in the cavity 5 and individual bands 20, 21, 22 can be seen on the reaction strip 6.
  • the individual cavities 5, 7, 8 are marked by a numerical inscription 23 and the receiving unit 2 has a geometric marking 24, which is used as the basis for positioning the entire receiving unit 2. A corner of the receiving unit 2 is blunted as a geometric marking 24.
  • the reaction strip 6 lying in the cavity 5 has a light background, on which the bands 20, 21, 22 appear as darker discolorations.
  • the cavity 5 and the entire tub 2 are colored matt black in order to form a high contrast to the background of the reaction strip 6.
  • the reaction strip 6 is a Westerblot or Dotblot or another electrophoretically created strip.
  • the evaluation unit 4 uses known systems to assign findings and diagnoses to the optically recorded bands 20, 21, 22.
  • the strips 6 are provided with numbers 25.
  • the optical sensor 3 detects these numbers 25 and uses them to assign specific ones Examination data, such as laboratory identification numbers, surnames, first names, birth dates etc. This information increases the security of the processing and transmission of the information from the reaction strips 6.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

L'invention concerne un procédé pour l'analyse de bandelettes réactives selon lequel on place, pour les développer, les bandelettes réactives dans une unité réceptrice, en particulier une cuve, où elles sont exposées à des réactifs liquides. Dans le procédé selon l'invention, la surface des bandelettes réactives est détectée à l'aide d'un capteur optique et les données enregistrées sont ensuite transmises à une unité d'analyse. L'invention concerne également un dispositif pour l'exécution du procédé, comportant une cuve pourvue d'un capteur optique et d'une unité d'analyse.
PCT/DE1999/003272 1998-10-13 1999-10-12 Procede et dispositif pour l'analyse de bandelettes reactives Ceased WO2000022406A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP99959200A EP1119759A2 (fr) 1998-10-13 1999-10-12 Procede et dispositif pour l'analyse de bandelettes reactives

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19847061 1998-10-13
DE19847061.4 1998-10-13
DE19933168.5 1999-07-15
DE19933168A DE19933168A1 (de) 1998-10-13 1999-07-15 Verfahren und Vorrichtung zur Analyse von Reaktionsstreifen

Publications (2)

Publication Number Publication Date
WO2000022406A2 true WO2000022406A2 (fr) 2000-04-20
WO2000022406A3 WO2000022406A3 (fr) 2000-10-12

Family

ID=26049476

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/003272 Ceased WO2000022406A2 (fr) 1998-10-13 1999-10-12 Procede et dispositif pour l'analyse de bandelettes reactives

Country Status (2)

Country Link
EP (1) EP1119759A2 (fr)
WO (1) WO2000022406A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE40768E1 (en) 1999-03-01 2009-06-23 Siemens Healthcare Diagnostics Inc. Optical inspection apparatus with removable inserts
JP2015055492A (ja) * 2013-09-10 2015-03-23 ニプロ株式会社 反応装置

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4755058A (en) * 1984-06-19 1988-07-05 Miles Laboratories, Inc. Device and method for measuring light diffusely reflected from a nonuniform specimen
DE3722271A1 (de) * 1986-07-08 1988-01-21 Bio Rad Laboratories Hochempfindlicher nachweis von antikoerpern, die eine aids-exposition anzeigen
US5182707A (en) * 1990-07-23 1993-01-26 Healthdyne, Inc. Apparatus for recording reagent test strip data by comparison to color lights on a reference panel
US5724148A (en) * 1996-05-09 1998-03-03 Bayer Corporation Apparatus and method for determination of urine color

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE40768E1 (en) 1999-03-01 2009-06-23 Siemens Healthcare Diagnostics Inc. Optical inspection apparatus with removable inserts
JP2015055492A (ja) * 2013-09-10 2015-03-23 ニプロ株式会社 反応装置

Also Published As

Publication number Publication date
EP1119759A2 (fr) 2001-08-01
WO2000022406A3 (fr) 2000-10-12

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