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WO2000017365A3 - Binary viral expression system in plants - Google Patents

Binary viral expression system in plants Download PDF

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Publication number
WO2000017365A3
WO2000017365A3 PCT/US1999/021989 US9921989W WO0017365A3 WO 2000017365 A3 WO2000017365 A3 WO 2000017365A3 US 9921989 W US9921989 W US 9921989W WO 0017365 A3 WO0017365 A3 WO 0017365A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
expression system
viral expression
binary
binary viral
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/021989
Other languages
French (fr)
Other versions
WO2000017365A8 (en
WO2000017365A2 (en
Inventor
Narendra S Yadav
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Priority to NZ510594A priority Critical patent/NZ510594A/en
Priority to EP99969445A priority patent/EP1115870A2/en
Priority to AU14410/00A priority patent/AU1441000A/en
Priority to HU0104697A priority patent/HUP0104697A3/en
Priority to CA002339595A priority patent/CA2339595A1/en
Publication of WO2000017365A2 publication Critical patent/WO2000017365A2/en
Publication of WO2000017365A3 publication Critical patent/WO2000017365A3/en
Publication of WO2000017365A8 publication Critical patent/WO2000017365A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/12011—Geminiviridae
    • C12N2750/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to plant transgene expression systems comprising chromosomally-integrated components that are individually heritable. The systems are useful for the regulated expression of foreign genes through gene amplification in plant tissue.
PCT/US1999/021989 1998-09-23 1999-09-22 Binary viral expression system in plants Ceased WO2000017365A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
NZ510594A NZ510594A (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
EP99969445A EP1115870A2 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
AU14410/00A AU1441000A (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
HU0104697A HUP0104697A3 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants
CA002339595A CA2339595A1 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US10155898P 1998-09-23 1998-09-23
US60/101,558 1998-09-23
US13008699P 1999-04-20 1999-04-20
US69/130,086 1999-04-20
US15025599P 1999-08-23 1999-08-23
US60/150,255 1999-08-23

Publications (3)

Publication Number Publication Date
WO2000017365A2 WO2000017365A2 (en) 2000-03-30
WO2000017365A3 true WO2000017365A3 (en) 2000-08-24
WO2000017365A8 WO2000017365A8 (en) 2000-10-12

Family

ID=27379204

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/021989 Ceased WO2000017365A2 (en) 1998-09-23 1999-09-22 Binary viral expression system in plants

Country Status (5)

Country Link
EP (1) EP1115870A2 (en)
CA (1) CA2339595A1 (en)
HU (1) HUP0104697A3 (en)
NZ (1) NZ510594A (en)
WO (1) WO2000017365A2 (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6632980B1 (en) 1997-10-24 2003-10-14 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
AU2001243939B2 (en) * 2000-03-28 2006-06-15 Queensland University Of Technology A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences
WO2001072996A1 (en) 2000-03-28 2001-10-04 Queensland University Of Technology A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences
US7468475B2 (en) 2000-06-16 2008-12-23 Schmuelling Thomas Method for modifying plant morphology, biochemistry and physiology
DE10049587A1 (en) 2000-10-06 2002-05-02 Icon Genetics Ag Vector system for plants
US7560622B2 (en) 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
DE10061150A1 (en) 2000-12-08 2002-06-13 Icon Genetics Ag Methods and vectors for the production of transgenic plants
DE10102389A1 (en) 2001-01-19 2002-08-01 Icon Genetics Ag Methods and vectors for plastid transformation of higher plants
DE10109354A1 (en) * 2001-02-27 2002-09-05 Icon Genetics Ag Recombinant viral switch systems
DE10114209A1 (en) 2001-03-23 2002-12-05 Icon Genetics Ag Site-directed transformation using amplification vectors
DE10115507A1 (en) 2001-03-29 2002-10-10 Icon Genetics Ag Method for coding information in nucleic acids of a genetically modified organism
DE10121283B4 (en) * 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Methods and vectors for amplification or expression of desired nucleic acid sequences in plants
DE10132780A1 (en) 2001-07-06 2003-01-16 Icon Genetics Ag Plastid gene expression via autonomously replicating vectors
DE10143237A1 (en) 2001-09-04 2003-03-20 Icon Genetics Ag Manufacture of artificial internal ribosomal entry point elements (Ires elements)
DE10143238A1 (en) 2001-09-04 2003-03-20 Icon Genetics Ag Identification of eukaryotic internal ribosome entry sites (IRES) elements
US7238854B2 (en) 2002-04-11 2007-07-03 E. I. Du Pont De Nemours And Company Method of controlling site-specific recombination
WO2004003180A1 (en) 2002-07-01 2004-01-08 E.I. Du Pont De Nemours And Company Method of controlling gene silencing using site-specific recombination
AU2007302306A1 (en) 2006-09-25 2008-04-03 Freie Universitat Berlin Transcriptional repressors of cytokinin signaling and their use
ES2385451T3 (en) 2009-07-10 2012-07-25 Freie Universität Berlin Rock2 and Rock3, two new variants of function gain of cytokinin receptors AHK2 and AHK3
EP2418283A1 (en) 2010-08-07 2012-02-15 Nomad Bioscience GmbH Process of transfecting plants
EP2584042A1 (en) 2011-10-17 2013-04-24 Nomad Bioscience GmbH Production, storage and use of cell wall-degrading enzymes
EP2647715A1 (en) 2012-04-03 2013-10-09 Nomad Bioscience GmbH Agrobacterium for transient transfection of whole plants
EP3097783B1 (en) 2015-05-26 2019-11-13 Nomad Bioscience GmbH Colicin m for the control of ehec
EP3372091A1 (en) 2017-03-07 2018-09-12 Nomad Bioscience GmbH Method of reducing contamination of an object with clostridium
EP3378485A1 (en) 2017-03-24 2018-09-26 Nomad Bioscience GmbH Bacteriocins for control of salmonella enterica
US11633467B2 (en) 2018-02-15 2023-04-25 Icon Genetics Gmbh Immunogenic composition and vaccine for generating an immune response to norovirus
KR20220021451A (en) 2019-03-12 2022-02-22 아이콘 제네틱스 지엠비에이치 Norovirus-like particles with improved stability
US20220227818A1 (en) 2019-06-06 2022-07-21 Nomad Bioscience Gmbh Klebicins for the control of klebsilella
CA3189366A1 (en) 2020-07-16 2022-01-20 Nomad Bioscience Gmbh Products for oral consumption with reduced sugar content
EP4248987A1 (en) 2022-03-21 2023-09-27 Nomad Bioscience GmbH Chimeric bacteriocins and method for the control of pseudomonas

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0221044A1 (en) * 1985-10-25 1987-05-06 Monsanto Company Novel plant vectors
EP0425004A2 (en) * 1989-10-03 1991-05-02 Aveve N.V. Genetic manipulations with recombinant DNA comprising sequences derived from RNA virus
WO1994019477A1 (en) * 1993-02-26 1994-09-01 Calgene Inc. Geminivirus-based gene expression system
WO1995025801A2 (en) * 1994-03-23 1995-09-28 University Of Leicester Viral replicon
WO1998028431A1 (en) * 1996-12-24 1998-07-02 Plant Bioscience Limited Transcriptional regulation in plants
WO1998036083A1 (en) * 1997-02-14 1998-08-20 Plant Bioscience Limited Methods and means for gene silencing in transgenic plants
WO1999025855A1 (en) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from t-dna using site-specific recombination systems

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0221044A1 (en) * 1985-10-25 1987-05-06 Monsanto Company Novel plant vectors
EP0425004A2 (en) * 1989-10-03 1991-05-02 Aveve N.V. Genetic manipulations with recombinant DNA comprising sequences derived from RNA virus
WO1994019477A1 (en) * 1993-02-26 1994-09-01 Calgene Inc. Geminivirus-based gene expression system
WO1995025801A2 (en) * 1994-03-23 1995-09-28 University Of Leicester Viral replicon
WO1998028431A1 (en) * 1996-12-24 1998-07-02 Plant Bioscience Limited Transcriptional regulation in plants
WO1998036083A1 (en) * 1997-02-14 1998-08-20 Plant Bioscience Limited Methods and means for gene silencing in transgenic plants
WO1999025855A1 (en) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from t-dna using site-specific recombination systems

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANGELL S M ET AL: "Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA.", EMBO JOURNAL, (1997 JUN) 16 (12) 3675-84., XP002135935 *
HAYES R J ET AL: "Replication of tomato golden mosaic virus DNA B in transgenic plants expressing open reading frames (ORFs) of DNA A: requirement of ORF AL2 for production of single-stranded DNA", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 17, no. 24, 25 December 1989 (1989-12-25), pages 10213 - 10222, XP002093974, ISSN: 0305-1048 *
HONG Y ET AL: "Transactivation of dianthin transgene expression by African cassava mosaic virus AC2", VIROLOGY,US,ACADEMIC PRESS,ORLANDO, vol. 228, no. 2, 17 February 1997 (1997-02-17), pages 383 - 387, XP002093972, ISSN: 0042-6822 *
PRUSS G. ET AL.: "Plant viral synergism: The potyviral genome encodes a broad-range pathogenicity enhancer that transactivates replication of heterologous viruses", PLANT CELL, vol. 9, 1997, pages 859 - 868, XP002078748 *

Also Published As

Publication number Publication date
NZ510594A (en) 2002-09-27
WO2000017365A8 (en) 2000-10-12
HUP0104697A2 (en) 2002-03-28
WO2000017365A2 (en) 2000-03-30
CA2339595A1 (en) 2000-03-30
EP1115870A2 (en) 2001-07-18
HUP0104697A3 (en) 2003-12-29

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