[go: up one dir, main page]

WO2000012677A3 - Generation of recombinant infectious bursal disease viruses by reverse genetics technology and the use of the recombinant viruses as attenuated vaccines - Google Patents

Generation of recombinant infectious bursal disease viruses by reverse genetics technology and the use of the recombinant viruses as attenuated vaccines Download PDF

Info

Publication number
WO2000012677A3
WO2000012677A3 PCT/IB1999/001604 IB9901604W WO0012677A3 WO 2000012677 A3 WO2000012677 A3 WO 2000012677A3 IB 9901604 W IB9901604 W IB 9901604W WO 0012677 A3 WO0012677 A3 WO 0012677A3
Authority
WO
WIPO (PCT)
Prior art keywords
viruses
generation
recombinant
strains
attenuated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB1999/001604
Other languages
French (fr)
Other versions
WO2000012677A2 (en
Inventor
Boon-Leong Lim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Hong Kong HKU
Original Assignee
University of Hong Kong HKU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Hong Kong HKU filed Critical University of Hong Kong HKU
Publication of WO2000012677A2 publication Critical patent/WO2000012677A2/en
Publication of WO2000012677A3 publication Critical patent/WO2000012677A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/10011—Birnaviridae
    • C12N2720/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The invention relates to the generation and mutagenesis of recombinant infectious bursal disease virus by reverse genetics technology. Site-directed mutagenesis of certain amino acid residues on the Non-CEF adapted, very virulent strains of IBDV transforms the viruses into attenuated, CEF-adapted strains. The attenuated, CEF-adapted strains can be used as live vaccines against the very virulent strains. Curently available live attenuated vaccines were generated by serial passage through cell culture and the like, whereas the present invention provides a novel molecular mechanism in manipulation of the viral genome and the generation of attenuated vaccines by site-directed mutagenesis. The same approach can be used to produce vaccine strains from newly evolved IBDV viruses or to manipulate the antigenicity and pathogenicity of the virus.
PCT/IB1999/001604 1998-09-01 1999-09-01 Generation of recombinant infectious bursal disease viruses by reverse genetics technology and the use of the recombinant viruses as attenuated vaccines Ceased WO2000012677A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9868498P 1998-09-01 1998-09-01
US60/098,684 1998-09-01

Publications (2)

Publication Number Publication Date
WO2000012677A2 WO2000012677A2 (en) 2000-03-09
WO2000012677A3 true WO2000012677A3 (en) 2000-06-29

Family

ID=22270458

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1999/001604 Ceased WO2000012677A2 (en) 1998-09-01 1999-09-01 Generation of recombinant infectious bursal disease viruses by reverse genetics technology and the use of the recombinant viruses as attenuated vaccines

Country Status (1)

Country Link
WO (1) WO2000012677A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6485940B2 (en) 2000-07-07 2002-11-26 Akzo Nobel N.V. Broad spectrum infectious bursal disease virus vaccine
EP1170302B1 (en) * 2000-07-07 2006-06-21 Intervet International BV Infectious bursal disease virus vaccines
WO2004098530A2 (en) * 2003-05-05 2004-11-18 Dow Agrosciences Llc Stable immunoprophylactic and therapeutic compositions derived from transgenic plant cells and methods for production
US20050214316A1 (en) * 2003-11-13 2005-09-29 Brown Thomas P Methods of characterizing infectious bursal disease virus
CN102258777B (en) * 2011-06-30 2012-09-26 河南农业大学禽病研究所 Method for preparing inactivated vaccine and combined vaccine by breeding infectious bursal disease virus (IBDV) by chicken embryo source cell line
EP3620505A1 (en) 2018-09-07 2020-03-11 Henkel AG & Co. KGaA Mixed metal colloids as bleach boosting agents
EP3620504A1 (en) 2018-09-07 2020-03-11 Henkel AG & Co. KGaA Platinum colloids as bleach boosting agents

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986007060A1 (en) * 1985-05-30 1986-12-04 Commonwealth Scientific And Industrial Research Or Cloning and expression of host-protective immunogens of ibdv
WO1991016925A1 (en) * 1990-05-04 1991-11-14 University Of Maryland At College Park Specific dna sequences related to an ibdv protein including vectors, hosts and vaccines
WO1995026196A1 (en) * 1994-03-29 1995-10-05 The University Of Maryland College Park CHIMERIC INFECTIOUS BURSAL DISEASE VIRUS cDNA CLONES, EXPRESSION PRODUCTS AND VACCINES BASED THEREON
WO1998009646A1 (en) * 1996-09-05 1998-03-12 University Of Maryland - Biotechnology Institute A method for generating birnavirus from synthetic rna transcripts

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986007060A1 (en) * 1985-05-30 1986-12-04 Commonwealth Scientific And Industrial Research Or Cloning and expression of host-protective immunogens of ibdv
WO1991016925A1 (en) * 1990-05-04 1991-11-14 University Of Maryland At College Park Specific dna sequences related to an ibdv protein including vectors, hosts and vaccines
WO1995026196A1 (en) * 1994-03-29 1995-10-05 The University Of Maryland College Park CHIMERIC INFECTIOUS BURSAL DISEASE VIRUS cDNA CLONES, EXPRESSION PRODUCTS AND VACCINES BASED THEREON
WO1998009646A1 (en) * 1996-09-05 1998-03-12 University Of Maryland - Biotechnology Institute A method for generating birnavirus from synthetic rna transcripts

Also Published As

Publication number Publication date
WO2000012677A2 (en) 2000-03-09

Similar Documents

Publication Publication Date Title
WO2003092592A3 (en) Dengue tetravalent vaccine containing a common 30 nucleotide deletion in the 3'-utr of dengue types 1,2,3, and 4, or antigenic chimeric dengue viruses 1,2,3, and 4
DE69842165D1 (en) PREPARATION OF ATTENUATED PARAIN FLUENZA VIRUS VACCINES FROM CLONED NUCLEOTIDE SEQUENCES
CA2366072A1 (en) Prrsv vaccines
ATE194657T1 (en) DEFECTIVE VIRUS VACCINE PRODUCED FROM A TRANSCOMPLEMENTARY CELL LINE
MY117686A (en) Infectious clones of rna viruses and vaccines and diagnostic assays derived thereof
ATE468858T1 (en) CHIMERIC VACCINES AGAINST FLAVIVIRUS
CA2370372A1 (en) Porcine reproductive and respiratory syndrome vaccine, based on isolate ja-142
CN110904153A (en) Construction method and application of recombinant porcine reproductive and respiratory syndrome virus for expressing African swine fever virus p12 or p17 protein
MY163972A (en) Attenuated recombinant newcastle disease virus and vaccine containing the same
Wang et al. Changes in VP2 gene during the attenuation of very virulent infectious bursal disease virus strain Gx isolated in China
WO2003023040A3 (en) Vaccinia virus mva-e3l-knockout-mutants and use thereof
WO2000012677A3 (en) Generation of recombinant infectious bursal disease viruses by reverse genetics technology and the use of the recombinant viruses as attenuated vaccines
UA84667C2 (en) Vaccine directed against infection induced by herpes virus of marek's disease
BR0012433A (en) Recombinant virus, infectious from infectious bursal disease, method to obtain the same, ibdv infectious mosaic (mibdv), and vaccine
DE69533067D1 (en) HEPATIS E-VIRUS ANTIGENE AND THEIR USE
RU96123539A (en) "TRIVAC (VIEV)" VACCINE FOR PREVENTION OF INFECTIOUS RHINOTRACHEITIS, PARAGRIPPA-3 AND VIRAL DIARRHIA-DISEASE OF CLOSE CATTLE
AU2403188A (en) Infectious bovine rhinotracheitis virus mutants, vaccines containing same, methods for the production of same and methods for the use of same
MXPA01005808A (en) Ibdv strain for in ovo administration.
AU4334193A (en) Infectious bovine rhinotracheitis virus mutants and vaccines
WO2001022916A3 (en) Novel hev antigenic peptide and methods
DE60143810D1 (en) VAKZINE AGAINST IPNV TO BE ISOLATED FROM HEFEZELLEN
WO1995003399A3 (en) Production method for preparation of disabled viruses
CA2363493A1 (en) Attenuated forms of bovine viral diarrhea virus
WO1996001615A3 (en) Hepatitis a virus deletion mutants and vaccine formulations containing the same
ATE342731T1 (en) VIRUS VACCINE CHARACTERIZED BY A BHV-1 GENE ELETION

Legal Events

Date Code Title Description
AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

122 Ep: pct application non-entry in european phase