WO2000007465A1 - Solution for resuscitating multicellular organism cells and method for resuscitating multicellular organism cells by using the same - Google Patents

Abstract

A method for resuscitating a multicellular organism tissue characterized by bringing into contact the multicellular organism tissue to be resuscitated which has been depressed, weakened or aged, compared with the conditions thereof in the vital state, with a resuscitating solution for multicellular organism tissues which contains a raw salt, a sugar in the equal or almost equal weight to sodium chloride contained in the raw salt and water soluble gelatin and is isotonic to the bodily fluid of the above multicellular organism tissue in the vital state at the optimum life temperature of the multicellular organism tissue to thereby increase the bound water in the tissue cells, thus restoring the vitality or youth of the tissue. Changes in the appearance and elasticity of the multicellular organism tissue which has been depressed, weakened or aged, compared with the conditions thereof in the vital state, with aging are relieved not only by merely supplying moisture to the cells in the tissue but also converting the thus supplied moisture into bound water and taking advantage of the phenomenon that sodium chloride diffuses in the bound water in the form of a gel so that normal ionic reactions are not inhibited, and, furthermore, activating reducing enzymes, thereby restoring the vitality or youth of the weakened or aged cells in the multicellular organism tissue.

Description

 TECHNICAL FIELD The present invention relates to a solution for resuscitation of cells of a multicellular organism and a method for resuscitation of cells of a multicellular organism using the solution.

 The present invention relates to a solution for resuscitation of a multicellular biological tissue (hereinafter referred to as a resuscitation solution) for restoring or regenerating a diminished or aged multicellular biological tissue of a human or animal to a state before diminishment. The present invention relates to a method for restoring or regenerating a deteriorated or aged multicellular biological tissue to a state before deterioration using the resuscitation fluid. In particular, the present invention relates to freshness or freshness of food such as cereals, potatoes, seeds, nuts, beans, seafood, beasts and whales, vegetables, fruits, mushrooms and algae. Fresh or poorly fresh multi-cellular biological tissues, and fresh or poorly fresh multi-cellular biological tissues in flora, flowers and real flowers, etc. A resuscitation fluid for restoring or rejuvenating a condition before freshness is lost, i.e., a condition before diminishing, and by using the resuscitation fluid, a degraded or aged multicellular biological tissue is restored to a condition before degradation. To rejuvenate or rejuvenate. Furthermore, the present invention relates to dermatitis or cell damage of human or animal cells, for example, cutaneous keratosis or atopic dermatitis such as octopus or sea urchin, or ulcers, keloids, wounds and Damaged human or animal multicellular biological tissue, such as wrinkles, can be converted to a multicellular biological tissue in a substantially intact state, and a weakened or aged multicellular biological tissue can be replaced with freshness, freshness, or youthfulness. The present invention relates to a resuscitation solution for a multicellular biological tissue such as a liniment, a lotion, etc., which can be converted into a multicellular biological tissue having a restored quality, and a method for reviving tissue cells of a noncellular multicellular organism using the solution. The present invention relates to a resuscitation solution for multicellular biological tissues, such as a liniment, a lotion, and the like, which converts freshness, freshness, or youthfulness into a restored multicellular biological tissue, and a method for regenerating tissue cells of a noncellular multicellular organism using the solution.

The present invention also relates to a remedy for cutaneous keratosis, keloids, wound scars, cirrhosis, gastric ulcer, pediatric asthma, atopic dermatitis and athlete's foot. Further, the present invention provides a hair growth Background Art

 When foods such as meat and seafood are stored for a long period of time, they are stored by low-temperature preservation methods such as refrigeration or freezing so that quality at the time of freshness is not lost. Cereals are stored in a closed atmosphere with reduced temperature and humidity. When storing food such as vegetables, storage methods such as refrigeration, freezing, or film packaging are used to maintain freshness.

 As described above, when preserving multicellular biological tissue, it is preserved to maintain the freshness or freshness of the multicellular biological tissue, for example, it is related to techniques for suppressing respiratory action and maintaining quality or quality deterioration Techniques have been used to inactivate enzymes.

 In addition, for foods such as ham and salted foods, high-concentration salt of 4% or more is used to reduce water activity to preserve foods. In addition, foods such as jams are preserved by adding sugar at a high concentration of 7% or more and converting free water into bound water. However, these preservation methods are designed to preserve the nutrients held by multicellular biological tissues by maintaining intracellular equilibrium with extracellular cells after transferring water from the cells outside the cells. It is not intended to preserve multicellular biological tissue itself.

 In addition, the skin that has deteriorated due to aging has a thinner epidermis and less moisture and subcutaneous fat, and it is easy for fine wrinkles to occur.Therefore, applying moisture to the skin by applying a moisturizing cream, etc. gives moisture to the skin. I try to remove small wrinkles.

Even when foods are stored by the above methods, the color, elasticity and water activity of the foods at the time of freshness or freshness cannot be maintained, which is a problem. Also, when applying a moisturizing cream to the skin to treat wrinkles on the skin, if the moisturizing cream is applied to the skin, the moisture of the skin can be increased and the skin can be given tension. However, wrinkles can be removed, but in the case of skin with poor elasticity, the moisturizing cream cannot enrich the skin's moisture and cannot give the skin tension. Wrinkles on the skin, which have become less elastic, cannot be removed and are a problem. Disclosure of the invention

 The present invention is intended to eliminate changes in appearance and elasticity of multicellular biological tissues due to changes in the appearance and elasticity of food or the like during storage or aging that are difficult to solve by conventional methods. The purpose is to achieve an improvement in the appearance of multicellular biological tissues and an improvement in the elasticity of multicellular biological tissues.

 The present inventor has found that, for example, the intracellular recovery or rejuvenation of decayed or aged multicellular biological tissue, such as skin with reduced elasticity, can be supplemented directly by nutrients in cellular respiration. The present inventors have found that this can be achieved by binding and hydration of water between cells and cells, leading to the present invention.

 The present invention addresses the problems of eliminating the change in appearance and elasticity of a food or the like during storage or the change in elasticity of a multicellular biological tissue due to aging with the conventional method. In addition to replenishing the water inside the cells, the replenished water is bound and hydrated, and the salt can be diffused even in the hydrated gel, and the property that ordinary ion reactions are not suppressed is used. Activating the reductase to recover or rejuvenate cells of a diminished or aged multicellular biological tissue, and further improve the appearance of the multicellular biological tissue and elasticity of the multicellular biological tissue. The goal is to solve the problem by improving it.

The present invention relates to a multicellular biological tissue containing sodium chloride, sugar, and water-soluble gelatin, wherein the multicellular biological tissue to be resuscitated is isotonic or substantially isotonic with a body fluid at the time of life. The present invention also relates to a resuscitation fluid containing sodium chloride, potassium chloride, calcium chloride, sugar, and water-soluble gelatin, and isotonic or substantially to the body fluid of the revived multicellular biological tissue at the time of activation. A resuscitation solution of a multicellular biological tissue characterized by being isotonic, and the present invention further provides a resuscitation multibody biological tissue containing a raw salt, sugar, and water-soluble gelatin. A resuscitation solution for a multicellular biological tissue characterized by being isotonic or substantially isotonic. Furthermore, the present invention provides a resuscitation solution having a sodium chloride content of 2.5 to 3.5% by weight. With a sugar content of 2.5 to 3.5% by weight, A polysaccharide containing 0.25 to 0.35% by weight of isotropic gelatin, and isotonic or nearly isotonic with body fluids of the revived multicellular biological tissue at the time of living. In the resuscitation fluid of cellular biological tissue.

 Further, the present invention provides a transplant organ containing sodium chloride, sugar and water-soluble gelatin, which is isotonic or substantially isotonic with a body fluid of the revived multicellular biological tissue at the time of life. A preservation solution, and the present invention is characterized in that it contains an original salt, sugar and water-soluble gelatin, and is isotonic or nearly isotonic with a body fluid of the revived multicellular biological tissue at the time of life. The present invention also provides a preservation solution for organs for transplantation, wherein the sodium chloride content is 2.5 to 3.5% by weight, and the sugar content is 2.5 to 3.5% by weight. The water-soluble gelatin content is 0.25 to 0.35% by weight and isotonic or almost isotonic with body fluids of living multicellular biological tissues to be revived. It is in the preservation solution of the organ for transplantation.

 Further, the present invention provides a multicellular biological tissue containing sodium chloride, sugar and water-soluble gelatin, which is isotonic or substantially isotonic with a body fluid of the revived multicellular biological tissue at the time of life. The present invention further provides sodium chloride, potassium chloride, calcium chloride, sugar, and water-soluble gelatin, and isotonic or nearly isotonic with respect to the body fluids of the resuscitated multicellular biological tissue in life. The present invention relates to a medicament for treating a lesion of a multicellular biological tissue characterized by being isotonic. Furthermore, the present invention relates to the life of a revived multicellular biological tissue containing raw salt, sugar and water-soluble gelatin. The present invention relates to a therapeutic agent for a lesion of a multicellular biological tissue characterized by being isotonic or substantially isotonic with respect to body fluid at the time. Hair restorer, athlete's foot remedy, Treatment for cirrhosis, treatment for gastric ulcer, treatment for pediatric asthma, treatment for atopic asthma, treatment for keloids, treatment for wounds, treatment for cutaneous keratosis or treatment for atopic dermatitis 13. The therapeutic agent for a lesion of a multicellular biological tissue according to any one of claims 9 to 12, wherein the agent has a sodium chloride content of 2 or 3. 5 to 3.5% by weight, the sugar content is 2.5 to 3.5% by weight, the water-soluble gelatin content is 0.25 to 0.35% by weight, A therapeutic agent for a lesion of a multicellular biological tissue characterized by being isotonic or substantially isotonic with the body fluid of the multicellular biological tissue during life.

In addition, the present invention provides a method for regenerating a multicellular biological tissue which has been weakened, weakened or aged compared to the time of living, and resuscitated under the optimal living environment temperature of the living organism. And contacting a resuscitation solution of a multicellular biological tissue that is isotonic or substantially isotonic with the body fluid of the multicellular biological tissue at the time of life to restore vitality or rejuvenation of the tissue. The present invention also relates to a method for resuscitation of a multicellular biological tissue which has been weakened or weakened or aged compared to the time of living, and which is revived. Resuscitation of a multicellular biological tissue containing sodium chloride, potassium chloride, calcium chloride, sugar and water-soluble gelatin at a temperature and being isotonic or almost isotonic with body fluids of the multicellular biological tissue at the time of life A method for resuscitation of a multicellular biological tissue, which comprises contacting a liquid to restore or rejuvenate the vitality of the tissue. Yeah, Su The multicellular biological tissue to be prepared contains an original salt, sugar, and water-soluble gelatin at the optimal living environment temperature of the organism, and is isotonic or substantially isotonic with the body fluid of the multicellular biological tissue at the time of life. The present invention relates to a method for resuscitation of a multicellular biological tissue, which comprises bringing a resuscitation solution of a multicellular biological tissue into contact with the tissue to restore or rejuvenate the vitality of the tissue. The regenerated multicellular organism tissue which has been weakened or weakened or aged, has a sodium chloride content of 2.5 to 3.5% by weight under the optimal living environment temperature of the organism, The content is 2.5 to 3.5% by weight, and the content of water-soluble gelatin is 0.25 to 0.35% by weight. A resuscitation solution of a multicellular biological tissue, which is isotonic or nearly isotonic, is brought into contact with the tissue to restore the vitality of the tissue. In resuscitation method tissue of a multicellular organism, characterized in that the rejuvenating.

Also, the present invention provides a revived multicellular biological tissue that has been weakened, weakened or aged compared to life, and contains sodium chloride, sugar and water-soluble gelatin at an optimum temperature. A method for resuscitating a multicellular biological tissue, comprising: contacting a resuscitation solution of a multicellular biological tissue that is isotonic with a body fluid at the time of living the tissue to increase bound water in the tissue cells; Further, the present invention comprises resuscitated multicellular biological tissue that has been weakened or weakened or aged compared to life, and contains sodium chloride, potassium chloride, calcium chloride, sugar and water-soluble gelatin at an optimum temperature. Contacting a resuscitation solution of the multicellular biological tissue, which is isotonic with the body fluid of the multicellular biological tissue at the time of life, to increase bound water in the tissue cells. The present invention also relates to a resuscitation method, further comprising the steps of: regenerating a multicellular biological tissue that has been weakened or weakened or aged, compared with a living body, and reconstituted with an original salt, and sodium chloride in the original salt at an optimal temperature. Contacting a resuscitation solution of a multicellular biological tissue containing an equal or approximately equal weight of sugar and water-soluble gelatin and isotonic with the body fluid of the multicellular tissue at the time of life, The present invention relates to a method for resuscitation of a multicellular biological tissue characterized by increasing bound water, and in addition, the present invention relates to a method for regenerating a multicellular biological tissue which has been weakened or weakened or aged, compared to life, and is resuscitated. The method for resuscitation of a multicellular biological tissue according to claim 18, which is an organ for transplantation, further comprising the present invention, which is weakened or weakened or aged compared to life. The revived multicellular biological tissue Under the optimal living environment temperature, the content of sodium chloride is 2.5 to 3.5% by weight, the content of sugar is 2.5 to 3.5% by weight, and the content of water-soluble gelatin is An amount of 0.25 to 0.35% by weight, and a resuscitation solution of a multicellular biological tissue, which is isotonic or substantially isotonic, is brought into contact with a body fluid of the multicellular biological tissue at the time of life, A method for resuscitating a multicellular biological tissue characterized by increasing intracellular bound water. The present invention also relates to a revived multicellular biological tissue that has been weakened, weakened or aged compared to life, and a bodily fluid that contains raw salt and contains the original salt at the optimal temperature. Contacting the isotonic first resuscitation solution of the multicellular biological tissue with the first resuscitation solution, then contacting the multicellular biological tissue with the first resuscitation solution with an equal or nearly equal weight of sugar and sodium chloride in the raw salt; By contacting a second resuscitation liquid of the multicellular biological tissue containing water-soluble gelatin and isotonic with the body fluid of the multicellular biological tissue at the time of life, to increase the bound water in the tissue cells A method for resuscitation of a multicellular biological tissue characterized by restoring or rejuvenating the vitality of the tissue. BEST MODE FOR CARRYING OUT THE INVENTION

 In the present invention, resuscitation refers to restoring or rejuvenating a weakened or weakened or aged multicellular biological tissue or a damaged weakened or weakened multicellular biological tissue. Means

In the present invention, the resuscitation solution of the multicellular biological tissue contains salt, sugar, and water-soluble gelatin, and isotonic with the body fluid of the revived multicellular biological tissue at the time of living. Is a nearly isotonic solution, and the salt in the solution is preferably an original salt in that it contains trace amounts of potassium, calcium and magnesium in addition to sodium. If sodium, potassium and calcium are contained, the sugar in the solution is preferably contained in the same weight as the sodium chloride in the solution. In the present invention, water activity refers to the ratio of the water vapor pressure of a multicellular biological tissue such as a food at a certain temperature to the saturated water vapor pressure at that temperature.For example, in a relatively narrow space, the food has an equilibrium water content. It is equal to the relative humidity (%) when the temperature is reached divided by 100.

 In the present invention, the multicellular biological tissue contains a raw salt, sugar and water-soluble gelatin at an optimal living environment temperature of the organism, By contacting a resuscitation solution of a multicellular biological tissue that is isotonic or substantially isotonic, water and sugar are penetrated into the multicellular biological tissue through a cell membrane to replenish the regenerated tissue, that is, resuscitation of the multicellular biological tissue, It can rejuvenate the tissue, such as restoring the vitality of the tissue, such as restoring the tone or color of the tissue to a fresh or fresh state, or removing fine wrinkles appearing on the surface of the tissue.

 In the present invention, a multicellular biological tissue refers to a multicellular biological tissue of a human or a non-human organism. In the present invention, when the multicellular biological tissue is a human, the resuscitation solution of the multicellular biological tissue includes a lotion.

 In the present invention, the raw salt contained in the resuscitation liquid is preferably sea salt produced from seawater. The salinity of seawater is not much different in the ocean, 3.5% of total salt and 2.6-2.7% of salt. Although sea salt is a trace amount compared to salt, it contains a lot of other elements. One example of major element ions and their contents in seawater is as follows. Table 1 Amount of main ions in seawater

Ion content (g / kg) ratio (%) ion content (g / kg) ratio ^{(%) Na + 10. 47 30.} 52% cr 18. 97 55. 17 g + + 1. 28 3. 73 so 4 - -2.65 7.70

Ca— 0.41 1.19 Hco _s- 0.14 0.30 K ⁺ 0.38 1.10 Br "0.065 0.19

Sr ^{+ +} 0.013 0. 04 B0 ₃ ——— 0.027 0.08 Examples of major element ions and their content in sea salt are shown in Table 2 below.

¾ 2

 Zion composition Salt composition

Η ₂ 0 2.34% H ₂ 0 2.34%

 I. Μ 0.01% I.M 0.01%

CI 59.13% CaS0 ₄ 0.14¾

Ca 0.04% MgCl ₂ 0.05%

 Mg 0.02¾ KC1

 K 0.02% NaCl 97. 38%

 Na 38. 31% Total 100. 00%

 Total 100. 00% Total 100. 00%

 M: Insoluble substance

 Table 3 shows an example of trace elements in seawater and their contents. C Table 3 Trace elements in seawater (mg / l)

 Vitamin content Vitamin content

 Si 0. 2-1. As 0. 01-0. 03

 A1 0.6.2.4. Cu 0. 01-0. 03

 F 0.3.-0.8 U 0.002

 Ba 0.2 Pb 0.000δ ~ 0.002

 And i 0. 1-0. 2 Ag 0. 001

 I Ο. Οδ Au 0.00000004

Rb 0.005 Ra 0.17x10-1 ¹

_C shows an example of trace elements Ion and content in sea salt in the following Table 4 Table 4

 Trace components Detection limit Analysis data

 Mercury (Hg) 0.0 δ p P m D

 Arsenic (A s) 0.0 δ p ρ m ND

 Cadmium (Cd) 0.01 ppm ND

 Lead (Pb) 0.10 p pm D

 Copper (C u) 0.01 p pm ND

 Chrome (Cr) 0.02 p pm ND

 Zinc (Zn) 0.02 p pm 0.07 p pm

 Iron (F e) 0.02 p pm 5.10 p p m

 Manganese (Mn) 0.01 p pm 0.27 p pm

 General bacteria 300 / g ND

 Coliform group 30/100 g ND

 * ND: below the detection limit In the resuscitation solution of the present invention, saccharides such as pentose and hexose such as glucose, mannose and fructose can be used for monosaccharides, and alcohol reducing sugars, sucrose and saccharides for polysaccharides. Non-reducing sugar disaccharides can be used.

 Trehalose is widely used as a sugar, but in the case of preserving meat, disaccharides such as sucrose and alcohol-reducing sugar, and monosaccharides such as glucose and fructose can be used. There is no taste, but in the case of fructos, taste will be added. In the case of preservation of fish and shellfish, 3% sugar is used as an active ingredient in the plastic liquid, but it is preferable to use a sugar mixture of 2.7% fructose and 0.3% trehalose. . When preserving rice, the most effective sugar is Treha Kuchiichisuka ^. Odors such as rice bran can be removed by adding salt.

When the resuscitation liquid is sprayed on the meat mass at a temperature of 8 ° C, the temperature of the core of the meat mass rises to 13 ° C after 15 minutes, and the temperature of the core of the meat mass is 15 minutes after spraying. ° C and drop to a temperature of 12 ° C 45 minutes after spraying, 60 minutes after spraying The temperature returned to 8 ° C. This phenomenon is considered to be due to the fact that the sugar in the resuscitation fluid was absorbed by the meat mass and an enzyme in the cells of the meat mass reacted.

 The sodium in the resuscitation fluid is related to the sodium ion source in the “ion pump” involved in cell absorption of sugar, and is required as a sodium ion source. Examples of ion pumps for transporting inorganic ions through the biological membrane of cells include a proton ion pump, a sodium ion pump, a potassium ion pump, and a calcium ion pump. It is necessary to contain an inorganic ion, and raw salt and the like are preferable as an active ingredient of the resuscitation liquid in that they contain these inorganic ions.

 The resuscitation liquid of the present invention is obtained by adding no additives having a sodium chloride content of 85% or more and a heavy metal ion content of 1 δ ppm or less, 2.5 to 3.δ parts by weight of sea salt, and saccharification of corn starch. 2.5 to 3.5 parts by weight of powdered saccharides such as trehalose and 0.25 to 0.35 parts by weight of water-soluble gelatin dissolved in water to give 3% raw salt, 3% saccharides, and water A 0.3% aqueous solution of neutral gelatin can be prepared to prepare a resuscitation solution.

 In the present invention, water-soluble gelatin is used as a support for nutritional substances. Thus, the use of water-soluble gelatin facilitates deposition on biological tissue and transport of water into the biological tissue.

 In the present invention, the optimal living environment temperature of a multicellular biological tissue refers to the optimal temperature in an environment in which the multicellular organisms are active, and is determined from the breeding period for animals and from the flowering period for plants, It means the temperature within the temperature range that is most suitable for exerting the effect of the enzyme involved, and varies depending on the type of multicellular organism. It is preferable to subject the multicellular biological tissue to a resuscitation solution at such an optimal living environment temperature because resuscitation can be achieved effectively. However, the treatment temperature with the resuscitation liquid also means the storage temperature such as the refrigeration temperature and the thawing temperature during storage. By refrigeration or thawing in contact with the resuscitation solution, refrigeration or thawing can be performed without damaging the multicellular biological tissue.

In the present invention, the weakened, weakened or aged, resuscitated multicellular biological tissue is resuscitated by contact with a resuscitation solution containing raw salts, sugars and water-soluble gelatin. In this case, immerse the multicellular biological tissue to be resuscitated in the resuscitation solution, or Material, for example, paper, etc. to contain a resuscitation solution to create a material impregnated with the resuscitation solution, wrap the multicellular biological tissue with the material impregnated with the resuscitation solution, and leave it for a certain period of time to remove the multicellular organism. Tissue cells can form a saturated state of bound water and resuscitate multicellular biological tissues to their living state.

 In the present invention, the temperature of the resuscitation solution is adjusted beforehand within the range of the optimal living environment temperature of the animal or plant tissue cells before the cell tissue of the animal or plant is treated. The resuscitation solution thus obtained is brought into contact with animal or plant tissue cells by immersion, coating, spraying, or adhesion, etc., and the free water in the tissue cells is bound and hydrated until the resuscitation solution becomes saturated. Continue contact with. In this method, for example, refrigerated

Alternatively, the thawed tissue cells can be refrigerated or thawed by immersion in a resuscitation solution, preferably for 30 seconds to 3 minutes.

 In this case, the frozen multicellular biological tissue is wrapped in water-retentive paper or a fibrous material impregnated with a resuscitation solution and wrapped in a refrigerator for 1 晚, preferably with a thawing time of 8 to 12 hours. Multiply and decompress.

 In the present invention, the multicellular biological tissue is brought into contact with a resuscitation liquid containing raw salt, sugar and water-soluble gelatin, which is isotonic or substantially isotonic with the body fluid of the revived multicellular biological tissue at the time of life. For example, at the time of distribution of food materials, the shelf life is good in a refrigerated state, there is no fading or change in freshness even after storage for more than one week, and the number of general viable bacteria after one week is the first It is less than 10 times the bacterial count. However, when not contacted with a resuscitation solution of the same material, the number of bacteria in a refrigerated state is 2-3 days, and the number of general viable bacteria on the second day reaches about 100 times the number of primary bacteria .

 In general, in the case of meat and seafood, the outflow of cell fluid and intercellular fluid decreases in taste and taste, but the meat and seafood treated with the resuscitation solution of the present invention remain stable even after a lapse of time. Almost no outflow of cell liquid was observed, and even after one week of refrigerated storage, the outflowed cell liquid, that is, drip was slight, and the original taste of meat and seafood was retained even after one week. be able to. However, in the case of meat and seafood that have not been treated with the resuscitation solution of the present invention, as in the general case, viable dripping is confirmed with the lapse of time, and freshness decreases. The quality deteriorates and the taste decreases.

The cells of animals that have ceased vital activity and have passed 4 to 5 hours after death However, even if the proteysis is rapidly reduced, the animal cells do not stop respiratory activity, and the temperature rise in the animal cells when the resuscitation solution of the present invention is applied may partially resume the cell activity It is considered to indicate that.

 The off-flavors of so-called food materials, such as off-flavors of grass-fed beef (grass fattening), off-flavors of built-in meat, and fishy odors in marine products such as shrimp, are thought to be caused by deterioration due to peroxidation. However, as a result of the resuscitation treatment using the resuscitation solution of the present invention, the disappearance of the off-flavor is considered that the deteriorated cells of the food material have returned to the original state. In addition, regarding the effect on so-called food materials such as meat, marine products, rice and vegetables, and on the so-called vital cells such as the human body and flowers, some of the vital activities of the cells will be resumed.

 In the present invention, the resuscitation solution can be sprayed directly on the skin, for example, and penetrated into the skin to promote the activation of the cells of the epidermis and the dermis, thereby regenerating the normal cells that should be formed originally. it can. Therefore, the formation of abnormal cells formed by some external factors can be normalized to form normal cells by absorbing the resuscitation fluid, thereby promoting the formation of normal cells. Example

Example 1

 Additive-free sea salt with sodium chloride content of 85% or more and heavy metal ion content of 15ppm or less, 2.δ to 3.5 parts by weight, trehalose 2.5 to 3.5 parts by weight, water-soluble 0.25 to 0.35 parts by weight of gelatin was dissolved in water to prepare an aqueous solution containing 3% of raw salt, 3% of saccharides, and 0.3% of water-soluble gelatin, and used as a resuscitation solution.

Example 2

 As a raw material, about 2.5 kg of frozen US beef fin was used. The resuscitation solution (sea salt content: 3% by weight, trehalose content: 3% by weight, water-soluble gelatin content: 0.3% by weight) prepared in Example 1 was used.

The resuscitation liquid, the temperature of which was adjusted to 5 ° C., was sprayed on about 2.5 kg of the frozen beef with a sprayer to gradually start thawing and coloring of the frozen surface of the frozen beef. Thereafter, the frozen beef was mixed with a water-retaining zara paper mixing bar impregnated with the resuscitation liquid. Wrap it again and spray the resuscitation solution, which has been temperature-controlled to 5 ° C, evenly with a sprayer.c The amount of the resuscitation solution used for spraying is about 2.5 kg of frozen beef meat mass. About 40 ml. In this way, the resuscitation liquid sprayed and wrapped in wet meat vapor is further wrapped in Saran wrap, refrigerated at a temperature of about 8 ° C for about 10 hours, and thawed. , Revived. The thawing time depends on the weight of the frozen beef and the storage temperature. The storage temperature is preferably between 0 and 15 ° C.

 At the time of this thawing, if vacuum packaging was used instead of Saran wrap packaging, the breathing of the meat would be difficult, and the surface of the thawed beef was discolored and turned brown. In this example, in the refrigerated storage process at about 8 ° C after thawing, even after one week, the drips did not flow out, the recovered color was maintained, and the beef flavor was not impaired. It has returned to a single touch. This is thought to be due to the fact that sea salt induced color development, which was combined with saccharides and gelatin to hydrate free water such as intercellular fluid to protect the cell state.

 In this example, imported beef was used. However, imported beef has a firmer touch than domestic beef due to breed and age issues. However, the effect of raw salt also made the meat softer.

 After resuscitation of the beef thawed in this example, the meat paper was removed, vacuum-packaged and snap frozen. This rapidly frozen beef was immersed in 39 ° C warm water and thawed in a vacuum package. In this case, it thawed quickly in 5 minutes. After thawing, the vacuum packaging was removed and the inside of the bag was examined, but no drips were seen. The reanimated beef taken out of the vacuum packaging was stored refrigerated at a temperature of about 8 ° C, but after one week, the color of the freshness was preserved and the flavor of the beef was not impaired. The feel was unique.

 In the case of the conventional beef fin, the yield of roast beef was 72 to 78% by weight, but in the case of the beef fin of this example, the yield of roast beef was 86 to 88%. % By weight, giving a fresh texture. , Comparative example

Frozen fillet from the same lot as the frozen US beef used in Example 2 was used as a comparative example. The quick-frozen comparative example was immersed in hot water at 39 ° C with vacuum packaging. Thawed. In the case of the comparative example, thawing took 45 minutes. In addition, when the vacuum packaging was removed and the inside of the bag was examined, it was confirmed that drips of 6.3% of the weight of the meat had flowed out. The comparative example taken out of the vacuum packaging was also stored refrigerated at a temperature of about 8 ° C, but began to turn brown in the evening of the second day. At this point, a portion of the beef was cut off and tasted, but the sense of juiciness was impaired, and the flavor was off-flavor due to deterioration of the meat. In addition, the fourth said that the whole was darkened, the rot started completely, and the odor became strong.

Example 3

 Approximately 2.5 kg of US chilled beef fins was used as a raw material. The resuscitation solution was the same as that used in Example 2 except that the temperature was adjusted to 22 ° C. The resuscitation solution prepared in Example 1 above (sea salt content: 3% by weight, trehalose Content: 3% by weight, water-soluble gelatin content: 0.3% by weight aqueous solution).

 A resuscitation liquid adjusted to a temperature of 22 ° C. is sprayed on about 2.5 kg of the refrigerated beef with a sprayer, and then the frozen beef is treated with a water-retentive zara paper meat impregnated with the resuscitation liquid. After wrapping, the resuscitation solution, which was adjusted to 22 ° C, was sprayed evenly with a sprayer. In this way, the resuscitation liquid was sprayed and wrapped in wet meat paper, and then wrapped in Saran wrap, refrigerated at a temperature of about 8 ° C for about 2 hours, and revived.

 When distributing by vacuum packaging, freeze quickly and then store in vacuum packaging. When thawing, be careful not to cause browning as in Example 2.

When not vacuum packing, wrap in Saran wrap and store refrigerated. In the case of refrigerated beef, since it is already saturated with the resuscitation liquid, immersing it in a liquid impregnated with meat paper will cause supersaturation, and the phenomenon that the color of the meat color once developed will fade Therefore, remove the meat paper and keep it refrigerated while wrapped in Saran wrap. Resuscitate about 1 hour for 1 kg of meat mass. In this example, in the refrigerated storage process at about 8 ° C after thawing, even after one week, the drips did not flow out, the recovered color was maintained, the beef flavor was not impaired, and It had a uniform feel. This is thought to be due to the fact that sea salt induced color development, which was combined with saccharides and gelatin to hydrate free water such as intercellular fluid to protect the cell state. In this example, imported beef was handled.However, imported beef has a firmer feel than domestic beef due to breed and age issues, but the meat quality has also become softer due to the effect of raw salt. .

Example 4

 Approximately 4.5 kg of domestic refrigerated beef loin (including blood) was used as a raw material. The resuscitation solution prepared in Example 1 (aqueous solution having a sea salt content of 3% by weight, a trehalose content of 3% by weight, and a water-soluble gelatin content of 0.3% by weight) was used. A resuscitation liquid adjusted to a temperature of 22 ° C is sprayed on about 4.5 kg of the refrigerated beef with a sprayer, and then the frozen beef is treated with a water-retentive zara sheet meat impregnated with the resuscitation liquid. After wrapping, the resuscitation solution, which was adjusted to 22 ° C, was sprayed evenly with a sprayer. In this way, the resuscitation liquid was sprayed and wrapped in wet meat paper, and further wrapped in Saran wrap, refrigerated at a temperature of about 8 ° C for about 6 hours, and revived.

 In this example, in the refrigerated storage process at about 8 ° C after thawing, even after one week, the drips did not flow out, the recovered color was maintained, the beef flavor was not impaired, and It had a uniform feel. This is thought to be due to the fact that sea salt induced color development, which was combined with saccharides and gelatin to hydrate free water such as intercellular fluid to protect the cell state.

 With regard to blood cohesion, the blood cohesion weakened after storage for 2 hours, but after 6 hours the blood cohesion was completely dispersed and the blood spots had disappeared. This is thought to be because the body fluid that had coagulated between the cells was hemolyzed again due to the penetration of the resuscitation fluid, dispersed, and absorbed into the original meat tissue.

Example 5

As raw materials, 12 pieces of about 500 to 800 g of sac of Mediterranean frozen tuna spine prunus, which turned brown in the process of frozen storage, were used. A resuscitation solution (sea salt content 3%, trehalose content 3%, acetic acid content 2% and water-soluble gelatin content 0.3%) adjusted to 37 ° C was added to each frozen tuna sac. The surface of the frozen tuna was sprayed with a sprayer to start thawing and coloring of the frozen surface of each saku of the frozen tuna. Then, wrap each frozen tuna sac in meat paper impregnated with resuscitation liquid, Then, the resuscitation solution was sufficiently sprayed from the surface and stored at about 11 ° C for 1.5 hours. After confirming that the resuscitation was progressing based on the degree of color development of the frozen tuna saku, the meat paper was removed, vacuum-packaged, and quickly frozen.

 The frozen packaged frozen tuna that had been resuscitated with the resuscitation solution was thawed by soaking it in about 30% warm water for about 3 minutes. Thawed tuna saku was cut into pieces and tasted.

 Those that were not resuscitated with the resuscitation solution turned brown in 15 minutes after thawing and were spoiled by the black remedy 3 It returned to its original color and was kept refrigerated at a temperature of about 8 ° C in that state, but did not turn brown after one week.

 Those that were not resuscitated with the resuscitation solution dipped as much as 13% by weight after thawing, but those that were resuscitated with the resuscitation solution showed almost no drip even after one week. Not seen, the surface of the tuna was elastic and kept fresh.

Example 6

 About 2 kg of Indonesian refrigerated southern tuna spine Naka Shimo, which shows blood and brown discoloration, was used as a raw material. The resuscitation solution was the same as that used in Example 4, and had a sea salt content of 3%, a trehalose content of 3%, an acetic acid content of 2%, and a water-soluble gelatin content of 0.3%.

 The Mediterranean refrigerated southern tuna spine Naka Shimo about 2 kg on the surface of a meat mass, 3 m. : The temperature-adjusted resuscitation liquid was sprayed with a sprayer to start the coloring of the surface of the refrigerated tuna meat mass. Thereafter, the meat mass of the refrigerated tuna was wrapped with meat paper impregnated with a resuscitation liquid, and the resuscitation liquid was sufficiently sprayed from the surface, and stored at about 11 ° C for one and a half hours. It was confirmed that resuscitation had progressed from the degree of color development of the refrigerated tuna meat mass, and the meat paper was removed and stored refrigerated.

The refrigerated tuna meat mass kept refrigerated can be tasted as it is, and the blood is dispersed and absorbed. In addition, the brown discolored portion also returned to its original southern tuna color, and no change was seen after one week. Generally, refrigerated tuna begins to change its color from brown to brown in the evening, and brown color spreads widely on the third day. No change was observed in the resuscitation-treated resuscitation fluid after one week.

Example 7

 10 kg of polished rice using 10 kg of long grain rice from Thailand was soaked for 30 minutes in 10 kg of 1st resuscitation liquid containing 2% by weight of sea salt adjusted to 63 ° C. . After washing with water, 1 part by weight of rice was immersed in 1.3 parts by weight of a second resuscitation liquid containing 0.3% by weight of trehalose and 0.1% by weight of water-soluble gelatin for 10 minutes and immersed. The rice was cooked in the second resuscitation liquid.

 Some of the cooked rice was eaten and the rest was sealed and frozen. Rice stored frozen is re-heated, for example, for 1-1.5 minutes in a microwave oven.Conventionally, Thai rice has a lower viscosity than Japonica seeds, and cooked rice has a loose texture. Although it was not ready to be made into, for example, “rice rice”, the resuscitation treatment using the resuscitation solution in this example allowed the viscosity of Thai rice to be adjusted according to the treatment time, and from the viewpoint of taste. The smell from the rice milling process or the bad storage condition of brown rice had disappeared.

 In the case of Thai rice, rice with a grain width of 1.5 mm expands only about 1.3 times (rice grain width of about 2.1 mm) in normal rice cooking. After rice was cooked, rice expanded to about 2.2 times (rice grain width about 2.7 mm), and even after standing at room temperature after cooking, the rice did not dry and remained stable in water . In addition, when frozen after cooking rice, it was observed that the water once released and shrunk, but in the process of spontaneous thawing, the water in the air was again sucked up to the saturated state, and the original volume was cooked as a fluff. It was confirmed that he would return to.

 Further, in this example, the peculiar smell of Thai rice, which is an oxidatively degraded smell of fatty acids, is also eliminated.

In addition, the rice that has been frozen after cooking is usually moisturized and loses its moisture, which makes it feel dry and dry.In this example, the Thai rice that has been frozen after cooking is cooked by heating when thawing in the microwave. Finishing and this state were maintained after a lapse of 12 hours. Example 8

 97 Years old domestic rice "Kirara" 10 kg was used. 10 kg of the polished rice was immersed in 30 kg of the first resuscitation liquid containing 1% by weight of sea salt adjusted to 54 ° C. for 30 minutes. After washing with water, 1 part by weight of rice was immersed in 1.3 parts by weight of a second resuscitation solution containing 0.3% by weight of trehalose and 0.2% by weight of water-soluble gelatin for 10 minutes, and Was cooked in the second resuscitation liquid.

 Some of the cooked rice was eaten and the rest was sealed and frozen. Rice rice stored frozen has been reheated for 1 to 1.5 minutes, for example, in a microwave oven. From 180 to 200%, the resuscitation-treated old rice in this example has no peculiar deteriorating odor called “bran odor”, and the expansion rate of cooked rice grains is 20%. It was 0 to 230%. In the case of old rice, water retention was considered to be a problem, but in the resuscitation-treated old rice in this example, water retention increased, for example, rice with a 1.5 mm rice grain width became 2.9 mm rice grain. The rice was expanded to the width, and rice with a fluffy texture was obtained.

 In the case of frozen rice, it is said that there is a problem in drying the rice after thawing and heating. However, even in the old rice of this example, it retains a swelling feeling even after keeping it warm for 12 hours after heating. Was.

Example 9

 As a raw material, 10 kg of regular domestic rice “Hitmebore” was used. The resuscitation process using the first resuscitation solution and the first resuscitation solution and the resuscitation process using the second resuscitation solution and the second resuscitation solution were performed in the same manner as in Example 8.

 In particular, in the case of frozen rice, the deterioration of taste (mainly with dryness) due to drying after thawing and heating was a problem, but the rice revived according to this example also maintains heat after heating in this regard. Even after a lapse of 12 hours in the state, the swelling feeling was maintained.

In addition, it usually takes two and a half minutes to heat the thawing range.However, in the case of rice that has been resuscitated in this example, the thawing time can be reduced to one and a half minutes, It has been found that 10 to 15 minutes after heating gives a more fluffy feeling. In other words, it seems that the resuscitation process has made it possible for rice to return to its original water-saturated state.

Example 1 0

 Fresh cucumber was used as a raw material. It was immersed in the resuscitation solution prepared in Example 1 at a temperature of 36 ° C. for 3 minutes. After immersion, if stored refrigerated, the color will not fade and the gloss will not be lost even if it is 10th. In addition, the crispness of the cucumber was unchanged, and it had a taste like eating a freshly cut cucumber.

Example 1 1

 Fresh herb leaves were used as raw materials. 1 Float for 1 hour in the resuscitation solution prepared in Example 1 adjusted to a temperature of 11 ° C, rinse once, then immerse in warm water at 35 ° C for 5 minutes, and finally dip in hot water at 60 ° C After that, it was taken out and stored refrigerated.

 Herbs were resuscitated 1-2 times a year for the purpose of preservation and use throughout the year. As a result, leaf vegetables can be stored refrigerated for three months.

Example 1 2

 The resuscitation solution prepared in Example 1 is applied once to the entire surface of the face, then dried, the patient goes to bed in the dried state, and is washed with warm water the next morning.

 From the first day, the applied part became tense, giving a so-called “tensiony feel”.

 2 From the statement, the applied part generates heat and obtains a heated touch (some people may not generate heat)

 According to 3 to 5, the heat generated in the applied area subsides and the state of tension starts to persist

After one week of application, the wrinkles on the outer corners of the eyes disappeared, and for a week after that, the application remained firm even after the application was stopped.

Example 1 3

 Apply the resuscitation liquid prepared in Example 1 to the stain on the arm surface, dry and leave it, then wash with lemon water, apply the resuscitation liquid again, dry, leave it, and wash it again with lemon water Then, I left.

Repeating this allowance twice a day in the morning and evening, Shimika measures 1 cm in size. On the 10th to 14th days, it was separated into multiple spots of 1 mm in size, and thereafter, the stains disappeared in 3 to 5 days.

 Although the mechanism by which this stain disappears has not been identified, the resuscitation fluid does not act on the epidermis tissue where the stain is formed or between the epidermis and the dermis tissue, but directly on the dermis tissue that forms new epidermis tissue. This is thought to be due to the formation of new epidermal tissue, which pushes the upper layer of stains to the surface.

 Percutaneous absorption, which was conventionally considered difficult due to the presence of sebum, etc., is possible because the resuscitation fluid is isotonic or nearly isotonic with body fluids, forming a state that is easy to absorb. In addition, the sugars contained in the resuscitation fluid and the sodium chloride having the same weight or almost the same weight as the sugars play the role of an ion pump for permeation through the cell membrane, and thus the aging cells In addition, it is thought that sugar, which is a respiratory nutrient of the cell, is directly sent to the cell to promote cell activation.

Example 1 4

 Conventionally, people who had difficulty using the skin lotion containing aloe extract to increase the moisturizing effect and had a less obvious effect were treated with the same treatment as in Example 12 above. Became moist. Microscopic observations showed that the stratum corneum, which had a rugged surface, was flattened with a smooth surface, indicating an improvement.

 The mechanism by which this dry skin changes to moist skin has not been confirmed, but the resuscitation solution increases the vitality of the dermal tissue, making it easier to take in nutrients supplemented by a normal diet into cells. It is thought that it is doing.

Example 1 5

 The scalp was sprayed with the resuscitation solution prepared in Example 1 once a day at a rate of 5 ml per day, and used for three or more months. Three weeks after the spraying of the resuscitation liquid started, the amount of oil and fat that had oozed out of the scalp was reduced, and some hair roots were regenerated. In the second month after that, hair growth and the development of fairly thick hair were observed. As a confirmation of the prognosis, observation was made 3 months after the start of spraying the resuscitation liquid, and it was confirmed that hair root formation was well and that new hair had increased.

Example 1 6

Athlete's foot treatment 3 ml of the resuscitation solution prepared in Example 1 was applied once daily to the affected part of the athlete's foot patient. Two weeks after the application of the resuscitation fluid, the affected area was shrunk, and the areas with minor symptoms were completely healed. Four to six weeks after the start of the treatment, the affected area was healed.

Example 1 7

 Treatment of cirrhosis

 In the hepatic artery of a patient with cirrhosis, the resuscitation fluid prepared in Example 1 above (water content of sea salt content: 3% by weight, trehalose content: 3% by weight, water-soluble gelatin content: 0.3% by weight) 5 ml was directly injected daily for 3 weeks. By injecting this resuscitation fluid, fibrotic liver cells began to normalize, and three weeks after the start of treatment, the fibrous portion of liver in cirrhosis had decreased by more than 80%.

Example 18 8 Resuscitation solution prepared in Example 1 (sea salt content: 3% by weight, trehalose content: 3% by weight, water-soluble gelatin content: 0.3% by weight) Liquid) 3 ml was sprayed once a day. One week after the start of resuscitation treatment, the ulcer part shrank, and two weeks after the start of the resuscitation treatment, the ulcer part completely disappeared. Example 1 9

 Treatment of childhood asthma or atopic asthma

 The resuscitation solution 5111 1 prepared in Example 1 was inhaled into the throat throat once each time to a child with asthma and childhood asthma. On the third day after inhaling the resuscitation fluid, the attack of asthma subsided.

Example 2 0

 Healing of keloids and scars

After bathing once a day on the patient's keloid, 0.5 ml of the resuscitation solution prepared in Example 1 was applied. One month after the application of the resuscitation solution, the keloid became thinner and new hair follicles c. Two months after the application of the resuscitation solution, the keloid scar became thinner, New skin. Four new hairs were regenerated. Example 2 1 Healing of cutaneous keratosis

 (1) 0.5 ml of the resuscitation solution prepared in Example 1 was applied to the inner part of the patient's back once a day after human bathing. Two weeks after the start of application of the resuscitation liquid, drying shrinkage was observed on the upper part of the sea bream. On the 17th day after the application of the resuscitation solution, the seaweed dropped off, and new normal skin cells were formed in the scars of the seaweed.

(2) After taking a bath once a day in this part of the patient, the resuscitation solution prepared in Example 1 above was used.

5 ml was applied. Ten days after the start of the application of the resuscitation solution, drying shrinkage was observed in the upper keratinized part, and the octopus dropped off 15 days after the start of the application of the resuscitation solution. New normal skin cells were formed at the shedding area.

Example 2 2

 Cure of atopic dermatitis

 After bathing once a day on the affected part of the patient with atopic dermatitis, 0.5 ml of the resuscitation solution prepared in Example 1 was spread on absorbent cotton and applied by tapping lightly. The day after starting the application of the resuscitation solution, the inflammation of the dermatitis subsided. After that, after taking a bath once a day, 0.5 ml of the resuscitation solution prepared in Example 1 was applied to the cotton wool by blotting and tapping to suppress the occurrence of new dermatitis. However, no new dermatitis occurred even after 3 months.

Example 2 3

 As organ preservation solution for transplantation

 An organ for organ transplantation, for example, a lung, a heart, a liver, a kidney, and a spleen were immersed in the resuscitation solution prepared in Example 1 above. In each case, activation of organ cells was observed. Conventionally, the organ was maintained in a normal state for three times, while the activity of the organ was maintained for about half. Industrial applicability

In the present invention, the resuscitation fluid contains salt, sugar, and water-soluble gelatin, and is a resuscitation fluid that is isotonic or nearly isotonic with the body fluid of the revived multicellular biological tissue at the time of life. , So that valuable food materials can be stored for a long time It can be stored in various conditions, making it possible to eat without waste, and plays a role in preserving food in times of poor harvest.

 Further, the present invention provides a method for activating a living activity of a multicellular biological tissue by bringing the resuscitation solution into contact with the multicellular biological tissue, and the normalization of the multicellular biological tissue is achieved. Various diseases can be treated by the conversion.

Claims

The scope of the claims 1. A resuscitation fluid for a multicellular biological tissue, comprising sodium chloride, sugar and water-soluble gelatin, and isotonic or substantially isotonic to the body fluid of the revived multicellular biological tissue at the time of life.  2. A multicellular cell that contains sodium chloride, potassium chloride, calcium chloride, sugar and water-soluble gelatin, and is isotonic or nearly isotonic with the body fluids of the revived multicellular biological tissue during life. Resuscitation fluid for biological tissue.  3. A resuscitation solution of a multicellular biological tissue, which contains original salt, sugar and water-soluble gelatin, and is isotonic or nearly isotonic with the body fluid of the revived multicellular biological tissue during life c  4. The content of sodium chloride is 2.5 to 3.5% by weight, the content of sugar is 2.5 to 3.5% by weight, and the content of water-soluble gelatin is 0.25 to 0%. .3 a resuscitation fluid of multicellular biological tissue, characterized in that it is 5% by weight and isotonic or nearly isotonic with the body fluid of the revived multicellular biological tissue during life.  5. The resuscitation solution for a multicellular biological tissue according to any one of claims 1 to 4, wherein the sugar is contained in an amount equal to or substantially equal to that of sodium chloride.  6. A preservation solution for organs for transplantation, containing sodium chloride, sugar and water-soluble gelatin, which is isotonic or nearly isotonic with the body fluids of the revived multicellular biological tissue during life.  7. For transplantation, characterized by containing sodium chloride, potassium chloride, calcium chloride, sugar and water-soluble gelatin, and being isotonic or almost isotonic with the body fluids of the revived multicellular biological tissue at the time of life Organ preservation solution.  8. A preservation solution for organs for transplantation, comprising a raw salt, sugar and water-soluble gelatin, and isotonic or substantially isotonic with body fluids of the revived multicellular biological tissue during life. 9. The content of sodium chloride is 2.5 to 3.5% by weight, the content of sugar is 2.5 to 3.5% by weight, and the content of water-soluble gelatin is 0.25 to A preservative solution for organs for transplantation, characterized in that it is 0.35% by weight and isotonic or nearly isotonic with body fluids of the revived multicellular biological tissue during life. 10. The preservation solution for an organ for transplantation according to any one of claims 6 to 9, wherein the sugar is contained in an amount equal to or substantially equal to the weight of sodium chloride.  11. A lesion of a multicellular biological tissue characterized by containing sodium chloride, sugar, and water-soluble gelatin, and being isotonic or nearly isotonic with body fluid at the time of life of the revived multicellular biological tissue. Therapeutic drugs.  1 2. It contains sodium chloride, potassium chloride, calcium chloride, sugar, and water-soluble gelatin, and is characterized by being isotonic or almost isotonic with the body fluids of living multicellular biological tissues to be revived. Treatment of lesions in multicellular biological tissues.  1 3. Production of revived multicellular biological tissues containing raw salt, sugar and water-soluble gelatin  Two A therapeutic agent for a lesion of a multicellular biological tissue, wherein the therapeutic agent is isotonic or nearly isotonic with a body fluid at the time of activation.  1 4. The content of sodium chloride is 2.5 to 3.5% by weight, the content of sugar is 2.5 to 3.5% by weight, and the content of water-soluble gelatin is 0.25 to 0.35% by weight, which is a remedy for a lesion of a multicellular biological tissue characterized by being isotonic or nearly isotonic with body fluids of the revived multicellular biological tissue at the time of life.  15. The therapeutic agent for lesions of a multicellular biological tissue according to any one of claims 11 to 14, wherein the saccharide is contained in an amount equal to or approximately equal to the weight of sodium chloride. .  1 6. Therapeutic agents for multicellular biological tissue lesions include hair growth-promoting agents, athlete's foot treatments, liver cirrhosis treatments, gastric ulcer treatments, pediatric asthma treatments, atopic asthma treatments, and keloids. The multicellular biological tissue according to any one of claims 9 to 12, wherein the multicellular biological tissue is a therapeutic agent for a wound, a therapeutic agent for a scar, a therapeutic agent for a cutaneous keratosis, or a therapeutic agent for atopic dermatitis. For the treatment of lesions.  1 7. A revived multicellular biological tissue that has been weakened, weakened or aged compared to life, contains sodium chloride, sugar, and water-soluble gelatin at an optimal living environment temperature of the living organism. A multicellular biological tissue characterized by restoring or rejuvenating the vitality of a multicellular biological tissue by bringing a resuscitation solution of the multicellular biological tissue, which is isotonic or substantially isotonic, into contact with a body fluid of the cellular biological tissue during life. Resuscitation method. 1 8. A revived multicellular organism that has weakened, weakened, or aged compared to life. The tissue contains sodium chloride, potassium chloride, calcium chloride, sugar, and water-soluble gelatin at the optimal living environment temperature of the living organism, and isotonic or substantially equal to the body fluid of the multicellular biological tissue at the time of living. A method for resuscitation of a multicellular biological tissue, which comprises contacting a resuscitation solution of a multicellular biological tissue, which is tonic, to restore or rejuvenate the vitality of the tissue. 1 9. The regenerated multicellular organism tissue that has been weakened, weakened or aged compared to the time of life, and contains the original salt, sugar, and water-soluble gelatin at the optimal living environment temperature of the organism. Contacting a resuscitation solution of a multicellular biological tissue that is isotonic or nearly isotonic with the body fluid of the biological tissue at the time of life to restore or rejuvenate the vitality of the multicellular biological tissue; Resuscitation method.  20. In revived multicellular biological tissues that have weakened, weakened or aged compared to their daily lives, the content of sodium chloride is 2.5 to 3.5% by weight under the optimal living environment temperature of the organisms. A sugar content of 2.5 to 3.5% by weight, a water-soluble gelatin content of 0.25 to 0.35% by weight, and A method for resuscitation of a multicellular biological tissue, which comprises bringing a resuscitation solution of a multicellular biological tissue, which is isotonic or substantially isotonic, into contact with the body fluid to restore or rejuvenate the vitality of the tissue.  21. The method for resuscitating a multicellular biological tissue according to any one of claims 17 to 20, wherein the sugar is contained in an amount equal to or substantially equal to the weight of sodium chloride. 2 2. A revived multicellular biological tissue that has weakened, weakened, or aged compared to life, and, at an optimal temperature, contains sodium chloride, sugar, and water-soluble gelatin, A method for resuscitating a multicellular biological tissue, comprising contacting a resuscitation solution of a multicellular biological tissue that is isotonic with a body fluid to increase bound water in the tissue cells.  2 3. A revived multicellular biological tissue that has weakened, weakened, or aged compared to life, and contains, at optimal temperature, sodium chloride, potassium chloride, calcium chloride, sugar, and water-soluble gelatin. A method for resuscitating a multicellular biological tissue, which comprises contacting a resuscitation liquid of a multicellular biological tissue that is isotonic with a body fluid of the cellular biological tissue at the time of life to increase bound water in the tissue cells. . 2 4. Revived multicellular organisms that have weakened, weakened or aged compared to their daily lives Contains, at an optimum temperature, a raw salt, an equal or nearly equal weight of sugar and water-soluble gelatin as sodium chloride in the raw salt, and is isotonic with the living body fluid of the multicellular biological tissue A method for resuscitating a multicellular biological tissue, comprising contacting a resuscitation solution of the multicellular biological tissue to increase bound water in the tissue cells.  2 5. The revived multicellular organism tissue that has weakened, weakened, or aged compared to life, has a sodium chloride content of 2.5 to 3.5% by weight at the optimal living environment temperature of the organism. A sugar content of 2.5 to 3.5% by weight, a water-soluble gelatin content of 0.25 to 0.35% by weight, and A method for resuscitating a multicellular biological tissue, comprising contacting a resuscitation solution of a multicellular biological tissue that is isotonic or substantially isotonic with the body fluid of the above, to increase bound water in the tissue cells. 26. The method for resuscitating a multicellular biological tissue according to any one of claims 22 to 25, wherein the method is characterized in that it is contained in a weight equal to or substantially equal to sugar and sodium chloride. 27. The multicellular biological tissue according to any one of claims 18 to 21, wherein the revived multicellular biological tissue that has been weakened, weakened, or aged compared to life is an organ for transplantation. Resuscitation method.  2 8. A resuscitated multicellular organism that has been weakened, weakened or aged compared to its life, and at optimal temperature, isotonic to the body fluids of the multicellular organism containing the raw salt at the time of life. Bringing the multicellular biological tissue into contact with the first resuscitation solution of the certain multicellular biological tissue, and then contacting the multicellular biological tissue with the first resuscitation solution with the same weight or almost equal weight of sugar and water-soluble as sodium chloride in the raw salt; Contacting a second resuscitation solution of a multicellular biological tissue containing gelatin and isotonic with the body fluid of the multicellular biological tissue at the time of life to increase bound water in the tissue cells, A method for resuscitating a multicellular biological tissue, which restores or rejuvenates tissue vitality.