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WO2000001804A3 - Nucleotide sequence encoding a protein with beta glucanase activity and use thereof - Google Patents

Nucleotide sequence encoding a protein with beta glucanase activity and use thereof Download PDF

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Publication number
WO2000001804A3
WO2000001804A3 PCT/EP1999/004110 EP9904110W WO0001804A3 WO 2000001804 A3 WO2000001804 A3 WO 2000001804A3 EP 9904110 W EP9904110 W EP 9904110W WO 0001804 A3 WO0001804 A3 WO 0001804A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
nucleotide sequence
sequence encoding
glucanase activity
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1999/004110
Other languages
French (fr)
Other versions
WO2000001804A2 (en
Inventor
Richard Kemp
Rebecca Stratford
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever NV
Original Assignee
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever NV filed Critical Unilever NV
Priority to AU45135/99A priority Critical patent/AU4513599A/en
Publication of WO2000001804A2 publication Critical patent/WO2000001804A2/en
Publication of WO2000001804A3 publication Critical patent/WO2000001804A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8235—Fruit-specific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01039—Glucan endo-1,3-beta-D-glucosidase (3.2.1.39)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A nucleotide sequence encoding for either a protein having an amino acid sequence with at least 40 % identity to Sequence ID no 1, or an active derivative of said protein or a fragment of said protein or an isoform of said protein. Also embraced is the use of said nucleotide sequence for isolating a regulatory sequence and a regulatory sequence thus obtained. Furthermore the invention relates to the use of said regulatory sequences for transforming plants.
PCT/EP1999/004110 1998-07-03 1999-06-14 Nucleotide sequence encoding a protein with beta glucanase activity and use thereof Ceased WO2000001804A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU45135/99A AU4513599A (en) 1998-07-03 1999-06-14 Nucleotide sequence

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98305338 1998-07-03
EP98305338.0 1998-07-03

Publications (2)

Publication Number Publication Date
WO2000001804A2 WO2000001804A2 (en) 2000-01-13
WO2000001804A3 true WO2000001804A3 (en) 2000-02-24

Family

ID=8234915

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/004110 Ceased WO2000001804A2 (en) 1998-07-03 1999-06-14 Nucleotide sequence encoding a protein with beta glucanase activity and use thereof

Country Status (2)

Country Link
AU (1) AU4513599A (en)
WO (1) WO2000001804A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT102979A (en) * 2003-06-26 2004-12-31 Castania Sociedade Agroflorest GENE ENCODING GENES OF CYANLASE (AOC), CISTATIN, BETA-1,3-GLUCANASEE OF TAUMATIN-LIKE PROTEIN ISOLATED FROM EUROPEAN CHESTNUT (CASTANEA SATIVA MILL.)
US7148052B2 (en) * 2003-08-26 2006-12-12 Chao-Ying Chen Nucleic acid encoding β-1,3-glucanase from lily
CN109762919B (en) * 2018-12-29 2022-04-08 浙江医药高等专科学校 Method for rapidly identifying raspberry and various confuses thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998053085A1 (en) * 1997-05-20 1998-11-26 Zeneca Limited Genetic control of fruit ripening

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998053085A1 (en) * 1997-05-20 1998-11-26 Zeneca Limited Genetic control of fruit ripening

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CASTRESANA, C. ET AL.: "Tissue-specific and pathogen-induced regulation of a Nicotiana plumbaginifolia beta-1,3-glucanase gene", THE PLANT JOURNAL, vol. 2, December 1990 (1990-12-01), pages 1131 - 1143, XP002103333 *
CLENDENNEN, S.K. AND MAY, G.D.: "Differential gene expression in ripening banana fruit", PLANT PHYSIOLOGY, vol. 115, October 1997 (1997-10-01), pages 463 - 469, XP002049417 *
TÖPFER, R. ET AL.: "Modification of plant lipid synthesis", SCIENCE, vol. 268, 5 May 1995 (1995-05-05), pages 681 - 685, XP002103336 *
WOLF, N.: "Structure of the genes encoding Hordeum vulgare (1->3,1->4)-beta-glucanase isoenzymes I and II and functional analysis of their promoters in barley aleurone protoplasts", MOLECULAR & GENERAL GENETICS, vol. 234, no. 1, July 1992 (1992-07-01), pages 33 - 42, XP002103334 *
YUN, S.J. ET AL.: "Sequence of a (1-3,1-4)-beta-glucanase cDNA from oat", PLANT PHYSIOLOGY, vol. 103, 1993, pages 295 - 296, XP002103335 *

Also Published As

Publication number Publication date
AU4513599A (en) 2000-01-24
WO2000001804A2 (en) 2000-01-13

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