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WO2000075362A3 - Method for identifying novel transcriptional regulatory proteins - Google Patents

Method for identifying novel transcriptional regulatory proteins Download PDF

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Publication number
WO2000075362A3
WO2000075362A3 PCT/IB2000/000859 IB0000859W WO0075362A3 WO 2000075362 A3 WO2000075362 A3 WO 2000075362A3 IB 0000859 W IB0000859 W IB 0000859W WO 0075362 A3 WO0075362 A3 WO 0075362A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
transcriptional regulatory
interest
transcription
regulatory proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2000/000859
Other languages
French (fr)
Other versions
WO2000075362A2 (en
Inventor
Wolfgang Hillen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU54177/00A priority Critical patent/AU5417700A/en
Publication of WO2000075362A2 publication Critical patent/WO2000075362A2/en
Anticipated expiration legal-status Critical
Publication of WO2000075362A3 publication Critical patent/WO2000075362A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for identifying transcriptional regulatory proteins that modulate the transcription of a gene of interest is disclosed. In one embodiment, the method comprises introducing into a cell a nucleic acid molecule comprising three central components: 1) a polynucleotide (e.g., DNA) encoding a transcriptional regulatory protein; 2) an indicator gene which is responsive to, e.g., under the transcriptional control of, the gene regulatory sequences of a gene of interest which bind to the transcriptional regulatory protein; and 3) a selectable marker gene. The transcriptional regulatory protein binds to the gene regulatory sequences and either activates or inhibits transcription. A protein is identified as a modulator of the transcription of the gene of interest by detecting a signal generated by the indicator gene.
PCT/IB2000/000859 1999-06-04 2000-06-05 Method for identifying novel transcriptional regulatory proteins Ceased WO2000075362A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU54177/00A AU5417700A (en) 1999-06-04 2000-06-05 Method for identifying novel transcriptional regulatory proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13767199P 1999-06-04 1999-06-04
US60/137,671 1999-06-04

Publications (2)

Publication Number Publication Date
WO2000075362A2 WO2000075362A2 (en) 2000-12-14
WO2000075362A3 true WO2000075362A3 (en) 2002-02-07

Family

ID=22478542

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2000/000859 Ceased WO2000075362A2 (en) 1999-06-04 2000-06-05 Method for identifying novel transcriptional regulatory proteins

Country Status (2)

Country Link
AU (1) AU5417700A (en)
WO (1) WO2000075362A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050266447A1 (en) * 2004-04-19 2005-12-01 Pioneer Hi-Bred International, Inc. Method for identifying activators of gene transcription

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040892A1 (en) * 1995-06-07 1996-12-19 Basf Ag Tetracycline regulated transcriptional modulators with altered dna binding specificities

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040892A1 (en) * 1995-06-07 1996-12-19 Basf Ag Tetracycline regulated transcriptional modulators with altered dna binding specificities

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BELLI G ET AL: "An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 4, 15 February 1998 (1998-02-15), pages 942 - 947, XP002176572 *
GOSSEN M ET AL: "Transcriptional activation by tetracyclines in mammalian cells.", SCIENCE, vol. 268, no. 5218, 1995, pages 1766 - 1769, XP000606285 *

Also Published As

Publication number Publication date
AU5417700A (en) 2000-12-28
WO2000075362A2 (en) 2000-12-14

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