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WO2000068415A3 - A method for measuring biomolecules - Google Patents

A method for measuring biomolecules Download PDF

Info

Publication number
WO2000068415A3
WO2000068415A3 PCT/CA2000/000540 CA0000540W WO0068415A3 WO 2000068415 A3 WO2000068415 A3 WO 2000068415A3 CA 0000540 W CA0000540 W CA 0000540W WO 0068415 A3 WO0068415 A3 WO 0068415A3
Authority
WO
WIPO (PCT)
Prior art keywords
vessel
reaction
reactant
amount
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA2000/000540
Other languages
French (fr)
Other versions
WO2000068415A2 (en
Inventor
Ronald R Marquardt
Zhibo Gan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Manitoba
Original Assignee
University of Manitoba
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Manitoba filed Critical University of Manitoba
Priority to EP00926619A priority Critical patent/EP1177443A2/en
Priority to AU45317/00A priority patent/AU4531700A/en
Publication of WO2000068415A2 publication Critical patent/WO2000068415A2/en
Publication of WO2000068415A3 publication Critical patent/WO2000068415A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Described herein is a method for detecting molecules with biological activity and determining the identity, amount and activity of same. The method involves the use of a vessel-based system which facilitates and simplifies the measurement of the amount and activity of enzymes, enzyme inhibitors, lectins, receptors and other biologically active substances comprising the steps of carrying out a biological reaction in a first vessel and then separating a labeled reactant from a labeled product by transferring a portion of the reaction mixture to a second vessel. Specifically, the surface of the first vessel is coated with a reactant, additional components are added, thereby forming a reaction mix, and the reaction is allowed to proceed for a given period of time. The soluble portion of the reaction mix is then transferred to a second vessel and the amount of reactant liberated into the reaction mix is counted.
PCT/CA2000/000540 1999-05-10 2000-05-10 A method for measuring biomolecules Ceased WO2000068415A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00926619A EP1177443A2 (en) 1999-05-10 2000-05-10 A method for measuring biomolecules
AU45317/00A AU4531700A (en) 1999-05-10 2000-05-10 A method for measuring biomolecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA2,270,639 1999-05-10
CA 2270639 CA2270639A1 (en) 1999-05-10 1999-05-10 A method for measuring biomolecules

Publications (2)

Publication Number Publication Date
WO2000068415A2 WO2000068415A2 (en) 2000-11-16
WO2000068415A3 true WO2000068415A3 (en) 2001-02-01

Family

ID=4163499

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2000/000540 Ceased WO2000068415A2 (en) 1999-05-10 2000-05-10 A method for measuring biomolecules

Country Status (4)

Country Link
EP (1) EP1177443A2 (en)
AU (1) AU4531700A (en)
CA (1) CA2270639A1 (en)
WO (1) WO2000068415A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017165665A1 (en) * 2016-03-23 2017-09-28 The General Hospital Corporation Assays and methods for detecting udp-glucose

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0296036A2 (en) * 1987-06-15 1988-12-21 Orgenics Ltd. Reversed competitive solid phase immunoassay for detecting single epitope, analytes and kit therefor
EP0308704A1 (en) * 1987-09-03 1989-03-29 Fuji Photo Film Co., Ltd. Heterogeneous enzymimmunoassay
EP0518557A2 (en) * 1991-06-10 1992-12-16 Eli Lilly And Company Assay method for hydrolytic enzymes
US5538858A (en) * 1994-04-08 1996-07-23 Pierce Chemical Company Rapid assay for radioactive determination of protein kinase activity
WO1997043438A1 (en) * 1996-05-14 1997-11-20 University Of Manitoba Solid-phase activity assay for biologically active substance

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0296036A2 (en) * 1987-06-15 1988-12-21 Orgenics Ltd. Reversed competitive solid phase immunoassay for detecting single epitope, analytes and kit therefor
EP0308704A1 (en) * 1987-09-03 1989-03-29 Fuji Photo Film Co., Ltd. Heterogeneous enzymimmunoassay
EP0518557A2 (en) * 1991-06-10 1992-12-16 Eli Lilly And Company Assay method for hydrolytic enzymes
US5538858A (en) * 1994-04-08 1996-07-23 Pierce Chemical Company Rapid assay for radioactive determination of protein kinase activity
WO1997043438A1 (en) * 1996-05-14 1997-11-20 University Of Manitoba Solid-phase activity assay for biologically active substance

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JONES LAURIE J; UPSON ROSALYN H; HAUGLAND ROSARIA P; PANCHUK-VOLOSHINA NATALIYA; ZHOU MINGJIE; HAUGLAND RICHARD P: "Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement", ANALYTICAL BIOCHEMISTRY, vol. 251, 1997, pages 144 - 152, XP002152059 *
TWINNING S S: "FLUORESCEIN ISOTHIOCYANATE-LABELED CASEIN ASSAY FOR PROTEOLYTIC ENZYMES", ANALYTICAL BIOCHEMISTRY, vol. 143, 1984, pages 30 - 34, XP000961435 *

Also Published As

Publication number Publication date
WO2000068415A2 (en) 2000-11-16
AU4531700A (en) 2000-11-21
EP1177443A2 (en) 2002-02-06
CA2270639A1 (en) 2000-11-10

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