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WO2000066773A3 - Method of detection of cytochrome b mutations in funghi leading to resistance against anti-fungal agents - Google Patents

Method of detection of cytochrome b mutations in funghi leading to resistance against anti-fungal agents Download PDF

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Publication number
WO2000066773A3
WO2000066773A3 PCT/GB2000/001620 GB0001620W WO0066773A3 WO 2000066773 A3 WO2000066773 A3 WO 2000066773A3 GB 0001620 W GB0001620 W GB 0001620W WO 0066773 A3 WO0066773 A3 WO 0066773A3
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WO
WIPO (PCT)
Prior art keywords
cytochrome
mutations
disclosed
resistance
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2000/001620
Other languages
French (fr)
Other versions
WO2000066773A2 (en
Inventor
John David Windass
Stephen Paul Heaney
Annabel Renwick
David Mark Whitcombe
Stephen Little
Neil James Gibson
Jane Theaker
Carole Patricia Stanger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Ltd
Original Assignee
Syngenta Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9910100.8A external-priority patent/GB9910100D0/en
Priority claimed from GB0006004A external-priority patent/GB0006004D0/en
Priority claimed from GB0007901A external-priority patent/GB0007901D0/en
Priority to BR0010163-0A priority Critical patent/BR0010163A/en
Priority to JP2000615395A priority patent/JP2002542803A/en
Priority to KR1020017013641A priority patent/KR20020008163A/en
Priority to CA002370117A priority patent/CA2370117A1/en
Application filed by Syngenta Ltd filed Critical Syngenta Ltd
Priority to EP00925493A priority patent/EP1181391A2/en
Priority to AU44214/00A priority patent/AU781410C/en
Publication of WO2000066773A2 publication Critical patent/WO2000066773A2/en
Publication of WO2000066773A3 publication Critical patent/WO2000066773A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/80—Cytochromes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention discloses methods which are particularly sensitive for detecting low frequencies of mutations in mitochondrially encoded genes, such as the cytochrome b gene, making these an especially useful and commercially important way of screening plant pathogenic fungi for the onset of fungicidal resistance wherein the resistance is due to a mutation in a mitochondrially encoded gene. The methods disclosed include single nucleotide polymorphism detection techniques especially PCR detection methods. Also disclosed are DNA sequences encoding part of the wild type and mutant cytochrome b sequences of a range of plant pathogenic fungi and the use of the sequence information to detect mutations giving rise to fungicidal resistance. Allele specific oligonucleotides and oligonucleotide probes, diagnostic primers and diagnostic kits are also disclosed.
PCT/GB2000/001620 1999-04-30 2000-04-26 Method of detection of cytochrome b mutations in funghi leading to resistance against anti-fungal agents Ceased WO2000066773A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU44214/00A AU781410C (en) 1999-04-30 2000-04-26 Methods
EP00925493A EP1181391A2 (en) 1999-04-30 2000-04-26 Method of detection of cytochrome b mutations in funghi leading to resistance against anti-fungal agents
BR0010163-0A BR0010163A (en) 1999-04-30 2000-04-26 Processes for detecting a fungal nucleic acid mutation, and for detecting fungal resistance to a stobilurin analogue, fungal DNA sequence encoding all or part of a cytochrome b protein, fungal cytochrome b protein, allele-specific oligonucleotide capable of binding to a fungal nucleic acid sequence, diagnostic primer or diagnostic oligonucleotide capable of binding to a template, one or more diagnostic primers, allele-specific oligonucleotide probe, diagnostic kit, processes for detecting pathogenic fungal resistance from plant to fungicide, to detect and quantify the frequency of a mutation, to select an active fungicide and its optimal levels of application, and to control the fungal infection of a crop, and, legibly, from a computer
JP2000615395A JP2002542803A (en) 1999-04-30 2000-04-26 Method
KR1020017013641A KR20020008163A (en) 1999-04-30 2000-04-26 Methods
CA002370117A CA2370117A1 (en) 1999-04-30 2000-04-26 Method of detection of cytochrome b mutations in funghi leading to resistance against anti-fungal agents

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GBGB9910100.8A GB9910100D0 (en) 1999-04-30 1999-04-30 Assay
GB0006004A GB0006004D0 (en) 2000-03-13 2000-03-13 Assay
GB0007901.2 2000-03-31
GB0006004.6 2000-03-31
GB9910100.8 2000-03-31
GB0007901A GB0007901D0 (en) 2000-03-31 2000-03-31 Methods

Publications (2)

Publication Number Publication Date
WO2000066773A2 WO2000066773A2 (en) 2000-11-09
WO2000066773A3 true WO2000066773A3 (en) 2001-10-11

Family

ID=27255594

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/001620 Ceased WO2000066773A2 (en) 1999-04-30 2000-04-26 Method of detection of cytochrome b mutations in funghi leading to resistance against anti-fungal agents

Country Status (8)

Country Link
EP (1) EP1181391A2 (en)
JP (1) JP2002542803A (en)
KR (1) KR20020008163A (en)
CN (1) CN1359425A (en)
AU (1) AU781410C (en)
BR (1) BR0010163A (en)
CA (1) CA2370117A1 (en)
WO (1) WO2000066773A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1118677A1 (en) * 2000-01-17 2001-07-25 Novartis AG Oligonucleotides useful in identifying fungicide resistant plant pathogenic fungi
JP2002142774A (en) * 2000-11-14 2002-05-21 Ss Pharmaceut Co Ltd Nucleic acid for detecting fungi and method for detecting fungi using the same
UA125210C2 (en) * 2016-03-16 2022-02-02 Басф Се APPLICATION OF TETRAZOLINONS TO CONTROL RESISTANT PHYTOPATHOGENIC MUSHROOMS ON CEREALS
CZ309242B6 (en) * 2017-10-31 2022-06-15 VÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o Kit for detecting G143A mutation causing resistance to QoI fungicides
CN112322709B (en) * 2020-11-23 2022-07-05 河北省农林科学院植物保护研究所 Method for rapidly identifying nucleotide point mutation of Cytb gene of potato late blight bacterium and resistance of Cytb gene to pyraclostrobin
CN118460782A (en) * 2024-07-09 2024-08-09 中国农业科学院植物保护研究所 RPA primers and probes, kits and methods for detecting wheat powdery mildew resistant to QoIs fungicides

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CORRAN AJ ET AL: "Approaches ti in-vitro Lead Generation for fungicide invention", PESTICIDE SCIENCE, vol. 54, 1998, pages 338 - 344, XP002164633 *
DI RAGO J-P AT AL: "Molecular basis for resistance to Myxothiazol, Mucidin (Strobilurin A), and stigmatellin", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 264, no. 24, August 1989 (1989-08-01), pages 14543 - 548, XP002164632 *
KRAICZY P ET AL: "The molecular basis for the natural resistance of the cytochrome bc1 complex from strobilurin-producing basidomycetes to centre Qp inhibitors", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 235, 1996, pages 54 - 63, XP000990816 *
NEWTON C R ET AL: "ANALYSIS OF ANY POINT MUTATION IN DNA. THE AMPLIFICATION REFRACTORY MUTATION SYSTEM (ARMS)", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 17, no. 7, 11 April 1989 (1989-04-11), pages 2503 - 2516, XP000141596, ISSN: 0305-1048 *
STEFFENS JJ ET AL: "Mechanisms of fungicide resistance in phytopathogenic fungi", CURRENT OPINION IN BIOCHEMISTRY, vol. 7, 1996, pages 348 - 55, XP000990845 *
ZHENG D ET AL: "Characterization of mitochondrial cytochrome b gene from Venturia inequalis", CURRENT GENETICS, vol. 32, November 1997 (1997-11-01), pages 361 - 66, XP000990849 *

Also Published As

Publication number Publication date
WO2000066773A2 (en) 2000-11-09
CN1359425A (en) 2002-07-17
AU781410C (en) 2007-01-25
KR20020008163A (en) 2002-01-29
CA2370117A1 (en) 2000-11-09
BR0010163A (en) 2002-01-15
AU4421400A (en) 2000-11-17
AU781410B2 (en) 2005-05-19
EP1181391A2 (en) 2002-02-27
JP2002542803A (en) 2002-12-17

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