[go: up one dir, main page]

WO2000066028A1 - Compositions pour congeler du sperme canin, procede de congelation de sperme canin utilisant ces compositions et procede d'insemination artificielle employant le sperme canin congele - Google Patents

Compositions pour congeler du sperme canin, procede de congelation de sperme canin utilisant ces compositions et procede d'insemination artificielle employant le sperme canin congele Download PDF

Info

Publication number
WO2000066028A1
WO2000066028A1 PCT/KR2000/000396 KR0000396W WO0066028A1 WO 2000066028 A1 WO2000066028 A1 WO 2000066028A1 KR 0000396 W KR0000396 W KR 0000396W WO 0066028 A1 WO0066028 A1 WO 0066028A1
Authority
WO
WIPO (PCT)
Prior art keywords
sperm
dog
freezing
composition
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2000/000396
Other languages
English (en)
Inventor
Hwa Sub Son
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1019990015147A external-priority patent/KR19990078560A/ko
Application filed by Individual filed Critical Individual
Priority to AU44351/00A priority Critical patent/AU773578B2/en
Priority to DE10084534T priority patent/DE10084534B4/de
Priority to GB0126189A priority patent/GB2364233B/en
Priority to JP2000614915A priority patent/JP2002543101A/ja
Publication of WO2000066028A1 publication Critical patent/WO2000066028A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes

Definitions

  • the present invention relates to compositions for freezing dog sperm, a method of freezing the dog sperm utilizing the compositions and an artificial insemination method employing the frozen dog sperm. More particularly, the present invention relates to compositions for freezing dog sperm in which the number of spermatozoa living in the sperm and the shape and viability thereof are retained when the sperm is thawed within a predetermined time after it is frozen, a method of freezing the dog sperm utilizing the compositions, and an artificial insemination method involving thawing the frozen dog sperm at a suitable place and time and inserting the same into the vagina of the female dog.
  • Background Art
  • diluent fluids applicable to pigs, horses and cattle have been developed and put into practice.
  • development of diluent fluids applicable to dog sperm has not yet matured.
  • a method of preserving animal sperm for a long time without loss of freshness would allow superior sperm to be used to fertilize female animals as necessary, which would be a great contribution to the development of livestock and the pet industry.
  • a freezing method is most suitable for preserving sperm for a long time.
  • composition for freezing dog sperm comprising, based on 100 ml of distilled water, 0.25 to 4.5 g of sodium citrate, 0.45 to 4.0 g of dextrose, 0.01 to 0.3 g of penicillin, 50,000 to 500,000 IU of streptomycin, 0.25 to 0.45 g of catalase, 0.045 to 0.25 g of yolk, 1.5 to 15 ml of glycerol and 0.02 to 0.1 ⁇ g of dog serum.
  • method for freezing dog sperm using the composition including the steps of (a) collecting male dog sperm by a massage method or electrical shock, (b) centrifuging the collected sperm to separate and remove the upper fluid leaving only the lower sperm, (c) mixing equal volumes of the lower sperm and a composition and primarily refrigerating the mixture, (d) further adding the composition to the mixture obtained in the step (c) to reduce the volumetric ratio of sperm to the composition to 1 :2, and secondarily refrigerating the resulting mixture, and (e) adding liquid nitrogen to the mixture obtained in the step (d) and freezing the mixture.
  • a method for artificial insemination of dogs including the steps of (a) examining an appropriate ovulatory phase of a female dog for improvement of a fertility rate, (b) adding the frozen sperm prepared by the method of claim 2 to a mixture of a 0.3 to 1.5 wt% sodium chloride solution and dog serum, heated to a temperature of 30 to 40° C, and thawing the same, (c) testing the mobility of spermatozoa in the thawed sperm through a microscope, and (d) injecting the thawed sperm into the vagina of a female dog and massaging the clitoris of the female dog.
  • the present inventor has extensively studied to develop a method for freezing dog sperm and compositions for use therein, and has found the fact that the dog sperm can be frozen for storage by combining a small amount of dog serum with a composition similar to a general diluent fluid used for cattle or pigs, to complete the invention.
  • compositions for freezing dog sperm will first be described.
  • compositions for freezing dog sperm according to the present invention are prepared by homogeneously mixing into 100 ml of distilled water, 0.25 to 4.5 g of sodium nitrate for regulating the buffering function, acidity (pH) and osmotic pressure of spermatozoa, 0.45 to 4.0 g of dextrose as a metabolism and energy source, 0.01 to 0.3 g of penicillin as an antibiotic for preventing infection, 50,000 to 500,000 IU of streptomycin, 0.25 to 0.45 g of catalase, 0.045 to 0.25 g of yolk,
  • composition 1.5 to 15 ml of glycerol and 0.02 to 0.1 ⁇ g of dog serum.
  • the thus prepared composition is frozen in units of about 2 ml to be thawed whenever it is to be used.
  • composition according to the present invention stores sperm for a long time while maintaining the viability and motility of spermatozoa contained in the frozen sperm, and contains dog serum exhibiting normal fertility and delivery rates after thawing.
  • spermatozoa die quickly when mixed with urine, it is preferred for a male dog to urinate before sperm is collected from the male dog. Then, the genital organ of the male dog is massaged using a test tube having an artificial vagina inserted therein to make the male dog ejaculate into the test tube. To catalyze the collection of sperm by this method, a female dog in heat is let to hover around the male dog. Otherwise, frozen cotton or a sponge containing a vaginal secretion of a female dog in heat is thawed and allowed to be smelled by the male dog when collecting sperm.
  • the sperm is divided into three samples collected at different tubes.
  • the sample of the sperm containing spermatozoa is the second one collected.
  • Ea h sample is collected at a time interval of about 10 to 20 seconds.
  • the interval between first and second samples is too short to be discernible.
  • the second sample is preferably used.
  • three samples are all collected in one test tube to be used.
  • the thus collected sperm is separated by means of a centrifuge, and the upper fluid is discarded with only the lower sedimentary sperm left. Centrifuging is performed at about 500 to 1100 RPM/minute for 6 minutes.
  • Equal volumes of the lower sedimentary sperm and the composition prepared as described above are mixed at room temperature and then the mixture is primarily refrigerated.
  • the refrigerating time is about 10 to 20 minutes and the refrigerating temperature is about 5° C.
  • a mixture of sperm and the composition in a volumetric ratio of 1 :2 is further mixed with the primarily refrigerated mixture at room temperature, and then secondarily refrigerated at the same temperature and for the same time.
  • the sperm and the composition are homogeneously mixed.
  • a predetermined amount of the secondarily refrigerated mixture is taken by means of a dropper and 2-3 drops are dropped to the surface of dry ice. Since the surface temperature of dry ice is about -79° C, the mixture is frozen to an extent. The frozen mixture is separated from dry ice and placed into a container having liquid nitrogen at about - 196° C to then be hermetically sealed, thereby completing the process of freezing dog sperm. This method is called a "pellet method" since the frozen mixture in the liquid nitrogen is pellet-shaped.
  • the dog sperm can be frozen by a method using a straw, which will now be described briefly.
  • the secondarily refrigerated mixture is subdivided into about 0.5 ml samples and put into straws. Every 20 to 30 straws are grouped and immersed in a container containing liquid nitrogen. When the straws are immersed into the container for freezing, they are gradually immersed, rather than immersed to the bottom of the container at once. First, they are immersed as deep as the upper portion of the container to then be left there for a predetermined time, then as deep as the middle of the container to then be left there for a predetermined time, and then as deep as the bottom of the container.
  • the thus frozen sperm can be carried and stored for a long time, and can be used for artificial insemination after being thawed as needed.
  • an artificial insemination method using the frozen sperm will be described.
  • a test is performed to determine whether the female dog is in an ovulatory phase.
  • the ovulatory phase test is performed by known methods in the art to which the present invention pertains, such as a vaginal cell test or a hormone test.
  • the frozen sperm is thawed by adding the frozen sperm to a mixture of 0.3 to 1.5 wt% sodium chloride solution and a small amount of dog sperm heating, the mixture heated to a temperature of 30 to 40 °C, which is advantageous for maintaining the viability and shape of spermatozoa.
  • the mixture may be directly heated, since it is quite difficult to appropriately control the temperature, it is preferred that an airtight container containing the mixture of sodium chloride solution and dog sperm is submerged in hot water at about 50° C and heated.
  • the number, shape and mobility of living spermatozoa are examined through a microscope. Successful artificial insemination depends on the number of spermatozoa moving forward. Spermatozoa are classified into four types based on their mobility.
  • Class 0 means the case where spermatozoa move their tales without forward movement
  • class 1 means the case where less than 20% of spermatozoa move forward
  • class 2 means the case where 20 to 50% of spermatozoa move forward
  • class 3 means the case where 50 to 80% of spermatozoa move forward
  • class 4 means the case where more than 80% of spermatozoa move forward.
  • the thawed sperm is injected into the vagina of the female dog determined to be in the ovulatory phase and the clitoris of the female dog is massaged, to complete artificial insemination.
  • the thawed sperm may be injected into the uterus of the female dog in a surgical manner.
  • the lower part of the navel is cut about 2 cm to raise the uterus and the sperm of the male dog is directly injected into the uterus of the female dog by means of an injection syringe. Then, the peritoneum, abdominal muscle and skin of the female dog are sewed in order.
  • a mixture having the following composition was homogeneously mixed using a mixer to prepare the composition for freezing dog sperm based on 100 ml of distilled water.
  • Equal volumes of the thus separated lower sperm and the composition prepared in the above-described manner were mixed at room temperature and then primarily refrigerated.
  • the refrigerating time was 10 to 20 minutes and the refrigerating temperature was about 5 ° C.
  • the composition was further added to the primarily refrigerated mixture to then be mixed such that the mixture ratio of the sperm to the composition by volume became 1:2. This resulting mixture was then secondarily refrigerated at the same temperature and for the same time.
  • a predetermined amount of the secondarily refrigerated mixture was taken by means of a dropper and 2-3 drops were dropped onto the surface of dry ice. Since the surface temperature of dry ice is about -79° C, the mixture was frozen to an extent. The frozen mixture was separated from the dry ice and placed into a container having liquid nitrogen of about - 196° C, to then be hermetically sealed, thereby completing the freezing process of dog sperm. Artificial insemination using frozen sperm
  • Thawing was performed such that the frozen sperm was added to a mixture of 0.3 to 1.5 wt% sodium chloride solution and a small amount of dog sperm, the mixture was heated to a temperature of 38 °C and was then let to sit for 5 hours.
  • the mobility of living spermatozoa was examined by placing the thawed sperm on a slide and observing the same through a microscope.
  • Successful artificial insemination depends on the number of spermatozoa moving forward. The percentage of spermatozoa moving forward was about 85 % , which is suitable for artificial insemination.
  • the thawed sperm was divided into three portions and then injected into the vaginas of female dogs determined to be in an ovulatory phase. Then, the clitoris of each of the female dogs was massaged for 5 minutes, to complete artificial insemination.
  • a pregnancy test was done 1 month after completion of artificial insemination, and all three female dogs turned out to be pregnant. Later, the three female dogs delivered 4, 6 and 9 healthy puppies, respectively .
  • EXAMPLE 2 the composition for freezing dog sperm was prepared in the same manner as in Example 1, with the exception of 0.02 ⁇ g of dog serum being used.
  • Example 2 the freezing of dog sperm and artificial insemination were performed using the composition, by the same process as described in Example 1.
  • Example 2 the same composition for freezing dog sperm as in Example 1 was prepared and the dog sperm was frozen in the same manner as in Example, with the exception of the volumetric ratio of the composition to the dog sperm being 1: 1. The dog sperm was collected in the same manner as in Example
  • the frozen sperm was thawed in the same manner as in Example 1 and the mobility of spermatozoa in the thawed sperm was tested.
  • the test result showed that the percentage of spermatozoa moving forward was less than 20% , which is not suitable for artificial insemination.
  • Example 2 the same composition for freezing dog sperm was prepared in the same manner as in Example 1 , with the exception of dog serum not being contained in the composition.
  • the freezing and thawing methods were the same as those in Example 1 , and the dog sperm was collected in the same manner as in Example 1 using the same male dog.
  • the mobility test of spermatozoa frozen and thawed according to this comparative example showed that the spermatozoa move with their tales only without forward movement, which is not suitable for artificial insemination.
  • the present invention provides a method for freezing dog sperm which is difficult to be frozen for storage in view of peculiarity of spermatozoa of the dog sperm, and compositions for use therein. Therefore, the dog sperm can be stored in a frozen state, thereby preserving the dog of good strain. Also, the frozen male dog sperm can be used for artificial insemination at an appropriate time and place, thereby enabling an increase in the birth rate.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Cell Biology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Surgical Instruments (AREA)

Abstract

L'invention concerne des compositions permettant de congeler du sperme canin, un procédé de congélation de sperme canin utilisant ces compositions et un procédé d'insémination artificielle employant le sperme canin congelé. La composition permettant de congeler du sperme canin renferme 0,25 à 4,5g de citrate de sodium, 0,45 à 4,0g de dextrose, 0,01 à 0,3g de pénicilline, 50 000 à 500 000 UI de streptomycine, 0,25 à 0,45g de catalase, 0,045 à 0,25g de jaune, 1,5 à 15ml de glycérol, et 0,02 à 0,1 ν)g de sérum canin. Le procédé de congélation de sperme canin de cette invention consiste tout d'abord a) à recueillir du sperme d'un chien mâle par massage ou application d'une décharge électrique, b) à centrifuger le sperme recueilli de manière à séparer le fluide et à fractionner le sperme resté au fond du tube, c) à mélanger ce sperme resté au fond du tube à la composition susmentionnée, selon un rapport volumétrique de 1:1, puis à réfrigérer ce mélange une première fois, d) à ajouter de nouveau ladite composition au mélange obtenu au cours de l'étape précédente, de sorte que le sperme et la composition peuvent se mélanger selon un rapport volumétrique de 1:2, ce mélange étant ensuite réfrigéré une seconde fois, et enfin e) à ajouter de l'azote liquide au mélange obtenu au cours de l'étape précédente avant de congeler celui-ci.
PCT/KR2000/000396 1999-04-28 2000-04-26 Compositions pour congeler du sperme canin, procede de congelation de sperme canin utilisant ces compositions et procede d'insemination artificielle employant le sperme canin congele Ceased WO2000066028A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU44351/00A AU773578B2 (en) 1999-04-28 2000-04-26 Compositions for freezing dog sperm, method of freezing the dog sperm utilizing the compositions and artificial insemination method employing the frozen dog sperm
DE10084534T DE10084534B4 (de) 1999-04-28 2000-04-26 Verfahren zum Einfrieren von Hundesamenflüssigkeit und Verwendung der eingefrorenen Hundesamenflüssigkeit
GB0126189A GB2364233B (en) 1999-04-28 2000-04-26 Composition for freezing dog sperm method of freezing the dog sperm utilizing the compositions and artificial insemination method employing e frozen dog spe
JP2000614915A JP2002543101A (ja) 1999-04-28 2000-04-26 犬精液冷凍用組成物、これを用いた犬精液冷凍方法及びこの冷凍犬精液を用いた人工授精方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR1999/15147 1999-04-28
KR1019990015147A KR19990078560A (ko) 1999-04-28 1999-04-28 개정액냉동방법및그정액을이용한인공수정방법그리고정액냉동을위한조성물
KR1020000021012A KR100320588B1 (ko) 1999-04-28 2000-04-20 개 정액 냉동용 조성물, 이를 이용한 개 정액 냉동방법 및이 냉동된 정액을 이용한 인공수정 방법
KR2000/21012 2000-04-20

Publications (1)

Publication Number Publication Date
WO2000066028A1 true WO2000066028A1 (fr) 2000-11-09

Family

ID=26635013

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2000/000396 Ceased WO2000066028A1 (fr) 1999-04-28 2000-04-26 Compositions pour congeler du sperme canin, procede de congelation de sperme canin utilisant ces compositions et procede d'insemination artificielle employant le sperme canin congele

Country Status (7)

Country Link
JP (1) JP2002543101A (fr)
KR (1) KR100320588B1 (fr)
CN (1) CN1170511C (fr)
AU (1) AU773578B2 (fr)
DE (1) DE10084534B4 (fr)
GB (1) GB2364233B (fr)
WO (1) WO2000066028A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101064415B1 (ko) * 2010-04-30 2011-09-14 (주)미래생명공학연구소 가축의 체외 수정용 정자 처리방법
CN104758084A (zh) * 2015-04-20 2015-07-08 陈绪凯 奶牛性控冻精人工授精的方法
ES2695248A1 (es) * 2017-06-26 2019-01-02 Spermbiobank Slu Metodo y kit de congelacion y transporte de semen

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100433280B1 (ko) * 2000-09-22 2004-05-31 대한민국 돼지정자 동결용 희석액 및 돼지정자 동결정액 제조방법
KR20030014891A (ko) * 2001-08-13 2003-02-20 (주)베티즌 개 정액 냉각용 및 동결용 조성물, 이를 이용한 개 정액냉각 및 동결 방법 그리고 냉각 및 동결된 정액을 이용한개 인공수정 방법
CN1331390C (zh) * 2003-12-24 2007-08-15 甘孜州家畜改良站 牦牛冻精制作方法
CN100405902C (zh) * 2004-02-20 2008-07-30 辽宁省辽宁绒山羊原种场 一种动物的冷冻精液及其制作方法
CN100563665C (zh) * 2006-09-11 2009-12-02 华中农业大学 一种奶牛性控精子冷冻保存精液稀释液
CN102379279A (zh) * 2011-11-17 2012-03-21 张大宇 用于藏獒精子保存的冷冻液
CN103141471B (zh) * 2013-02-01 2015-09-30 四川农业大学 一种昆明狼犬精液冷冻液配方及配制方法
CN103900884B (zh) * 2014-04-11 2016-09-14 深圳市博锐德生物科技有限公司 精浆复合质控品及其制备方法、试剂盒
AU2017345636B2 (en) * 2016-10-18 2022-11-17 Smartbow Gmbh Device for artificially inseminating a mammal
CN107251892A (zh) * 2017-05-25 2017-10-17 中国热带农业科学院热带作物品种资源研究所 一种适用于热带地区的鸡精液稀释液、制备及其应用方法
CN107494520B (zh) * 2017-09-25 2020-07-28 江苏农牧科技职业学院 一种犬用精液冷冻稀释剂及冷冻方法
KR102536863B1 (ko) 2019-11-25 2023-05-30 서울대학교산학협력단 양막유래 줄기세포-조정배지를 포함하는 동물의 정액 동결보호용 조성물 및 이의 제조방법

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4007087A (en) * 1975-10-17 1977-02-08 Gametrics Limited Sperm fractionation and storage
SU587934A1 (ru) * 1973-07-19 1978-01-15 Украинский Научно-Исследовательский Институт Разведения И Искусственного Осеменения Крупного Рогатого Скота Разбавитель спермы животных
SE7800809L (sv) * 1977-01-26 1978-07-27 Fisons Ltd Forfarande for framstellning av nya salter av bensopyranderivat
JPH0826901A (ja) * 1994-07-11 1996-01-30 Rikagaku Kenkyusho 精子低温保護剤
KR0160090B1 (ko) * 1995-05-01 1999-02-18 김광희 돼지 액상 정액 인공수정용 희석제

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU587934A1 (ru) * 1973-07-19 1978-01-15 Украинский Научно-Исследовательский Институт Разведения И Искусственного Осеменения Крупного Рогатого Скота Разбавитель спермы животных
US4007087A (en) * 1975-10-17 1977-02-08 Gametrics Limited Sperm fractionation and storage
SE7800809L (sv) * 1977-01-26 1978-07-27 Fisons Ltd Forfarande for framstellning av nya salter av bensopyranderivat
JPH0826901A (ja) * 1994-07-11 1996-01-30 Rikagaku Kenkyusho 精子低温保護剤
KR0160090B1 (ko) * 1995-05-01 1999-02-18 김광희 돼지 액상 정액 인공수정용 희석제

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101064415B1 (ko) * 2010-04-30 2011-09-14 (주)미래생명공학연구소 가축의 체외 수정용 정자 처리방법
CN104758084A (zh) * 2015-04-20 2015-07-08 陈绪凯 奶牛性控冻精人工授精的方法
ES2695248A1 (es) * 2017-06-26 2019-01-02 Spermbiobank Slu Metodo y kit de congelacion y transporte de semen
WO2019002640A1 (fr) * 2017-06-26 2019-01-03 Spermbiobank, Slu Méthode et kit de congélation et de transport de sperme

Also Published As

Publication number Publication date
KR100320588B1 (ko) 2002-01-12
GB2364233B (en) 2003-06-25
KR20000049647A (ko) 2000-08-05
GB2364233A (en) 2002-01-23
DE10084534B4 (de) 2007-09-13
CN1351484A (zh) 2002-05-29
DE10084534T1 (de) 2002-05-02
GB0126189D0 (en) 2002-01-02
JP2002543101A (ja) 2002-12-17
AU773578B2 (en) 2004-05-27
AU4435100A (en) 2000-11-17
CN1170511C (zh) 2004-10-13

Similar Documents

Publication Publication Date Title
AU773578B2 (en) Compositions for freezing dog sperm, method of freezing the dog sperm utilizing the compositions and artificial insemination method employing the frozen dog sperm
Al-Katanani et al. Pregnancy rates following timed embryo transfer with fresh or vitrified in vitro produced embryos in lactating dairy cows under heat stress conditions
Matheson et al. Frozen human semen for artificial insemination
Souza-Fabjan et al. Assessment of the reproductive parameters, laparoscopic oocyte recovery and the first embryos produced in vitro from endangered Canindé goats (Capra hircus)
Iritani et al. Fertilization in vitro of cattle follicular oocytes with ejaculated spermatozoa capacitated in a chemically defined medium
Ehling et al. Laparoscopical intrauterine insemination with different doses of fresh, conserved, and frozen semen for the production of ovine zygotes
Dávila et al. Reproduction in goats
US20030082509A1 (en) Compositions for freezing dog sperm and method of freezing the dog sperm utilizing the compositions and artificial insemination method employing the frozen dog sperm
RU2259809C2 (ru) Композиция для замораживания спермы собаки
Steyn Application of Artificial Insemination (AI) on commercial sheep and goat production
WO2000030441A1 (fr) Cryopreservation d'ovocytes et d'embryons et methodes de production d'animaux par cette technique
Udeh et al. Effects of type of extender and storage conditions on the motility of goat spermatozoa
US20100210898A1 (en) Mammalian reproduction
WO2001052640A1 (fr) Procede de production de betail avec selection du sexe et agent de traitement utilise a cette fin
Sheremeta et al. The reproductive function and the metabolic state of cows organism after giving an injection of a neurotropic and metabolic preparation in the last ten-day period of pregnancy
Holt Reproductive technologies
Yang et al. Asynchronous Embryo Transfer in Ewes
Anderson Identification of sex in mammalian embryos
CA2207013C (fr) Utilisation du spectre de frequences biologiques dans l'embryogenese animal
Drobnis et al. Detection of altered acrosomal physiology of cryopreserved human spermatozoa after sperm residence in the female reproductive tract
Hao Research on the Survival Rate of Vitrification Transplantation of Frozen Embryos in Mutton Sheep
JP2862321B2 (ja) 凍結ハムスター未受精卵の製造方法
Niżański Can we use reproductive biotechniques in cats like it is used in dogs?
Al-Essawe et al. Addition of seminal plasma reduces binding of stallion spermatozoa to bovine oocytes
Ishida et al. Non-surgical transfer of fresh or frozen-thawed ovine embryos by laparoscopy

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 00808000.3

Country of ref document: CN

AK Designated states

Kind code of ref document: A1

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase

Ref document number: 2000 614915

Country of ref document: JP

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 200126189

Country of ref document: GB

Kind code of ref document: A

RET De translation (de og part 6b)

Ref document number: 10084534

Country of ref document: DE

Date of ref document: 20020502

WWE Wipo information: entry into national phase

Ref document number: 10084534

Country of ref document: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 69(1) EPC, EPO FORM 1205 DATED 17.12.2002

122 Ep: pct application non-entry in european phase
REG Reference to national code

Ref country code: DE

Ref legal event code: 8607