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WO2000056903A3 - IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA) - Google Patents

IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA) Download PDF

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Publication number
WO2000056903A3
WO2000056903A3 PCT/US2000/007618 US0007618W WO0056903A3 WO 2000056903 A3 WO2000056903 A3 WO 2000056903A3 US 0007618 W US0007618 W US 0007618W WO 0056903 A3 WO0056903 A3 WO 0056903A3
Authority
WO
WIPO (PCT)
Prior art keywords
pichia
transformed
improved methods
producing proteins
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/007618
Other languages
French (fr)
Other versions
WO2000056903A2 (en
Inventor
Bruce L Zamost
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymogenetics Inc
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Priority to AU39100/00A priority Critical patent/AU3910000A/en
Publication of WO2000056903A2 publication Critical patent/WO2000056903A2/en
Publication of WO2000056903A3 publication Critical patent/WO2000056903A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/5759—Products of obesity genes, e.g. leptin, obese (OB), tub, fat
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Child & Adolescent Psychology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Endocrinology (AREA)
  • Botany (AREA)
  • Obesity (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methylotrophic yeasts are useful hosts for the production of commercially valuable recombinant proteins. However, the development of large-scale cultures of recombinant methylotrophic yeasts has been hindered by the formation of precipitation in culture media. A new soluble minimal medium overcomes this problem. Moreover, new feeding schemes provide cultures of high biomass, which produce biologically active recombinant protein.
PCT/US2000/007618 1999-03-22 2000-03-21 IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA) Ceased WO2000056903A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU39100/00A AU3910000A (en) 1999-03-22 2000-03-21 Improved methods for producing proteins in transformed (pichia)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27426399A 1999-03-22 1999-03-22
US09/274,263 1999-03-22

Publications (2)

Publication Number Publication Date
WO2000056903A2 WO2000056903A2 (en) 2000-09-28
WO2000056903A3 true WO2000056903A3 (en) 2000-12-28

Family

ID=23047491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/007618 Ceased WO2000056903A2 (en) 1999-03-22 2000-03-21 IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA)

Country Status (2)

Country Link
AU (1) AU3910000A (en)
WO (1) WO2000056903A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003509024A (en) * 1999-09-08 2003-03-11 ザイモジェネティクス，インコーポレイティド Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase 2 promoter and terminator
US6348331B1 (en) 1999-09-08 2002-02-19 Zymogenetics, Inc. Pichia methanolica glyceraldehyde-3-phosphate dehydrogenase 2 promoter
US7118901B2 (en) 2002-12-18 2006-10-10 Roche Diagnostics Operations, Inc. Recombinant bovine pancreatic desoxyribonuclease I with high specific activity
EP1460425A1 (en) 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
AT501955B1 (en) * 2005-02-23 2007-08-15 Univ Graz Tech MUTED AOX1 PROMOTERS
ES2370657T3 (en) 2005-09-14 2011-12-21 Sanofi-Aventis Deutschland Gmbh EXCISION OF INSULIN PRECURSORS THROUGH A VARIANT OF TRIPSINE.
EP2106447B1 (en) 2007-01-26 2013-04-17 Novozymes A/S Method for methanol independent induction from methanol inducible promoters in pichia
KR101283734B1 (en) * 2008-02-06 2013-07-08 바이오콘 리미티드 Fermentation medias and processes thereof
US20120213728A1 (en) * 2009-10-30 2012-08-23 Merck Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris
US11214610B2 (en) 2010-12-01 2022-01-04 H. Lundbeck A/S High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
JP6193220B2 (en) * 2011-05-20 2017-09-06 アルダーバイオ・ホールディングズ・エルエルシー High purity production of multi-subunit proteins such as antibodies in transformed microorganisms such as Pichia pastoris
AU2016273912B2 (en) * 2011-05-20 2018-08-02 H. Lundbeck A/S High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
US10150968B2 (en) 2011-08-19 2018-12-11 Alderbio Holdings Llc Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
ES2740449T3 (en) 2015-12-22 2020-02-05 Bisy E U Yeast cell
CN114686487B (en) * 2021-11-08 2023-12-22 泰州博莱得利生物科技有限公司 Efficient expression method of cat and dog interleukin 11 (IL-11) in pichia pastoris and application thereof
CN116103174B (en) * 2022-07-20 2024-11-29 青岛蔚蓝生物集团有限公司 A mutant strain of Pichia pastoris and its application in lysozyme production

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990003431A1 (en) * 1988-09-26 1990-04-05 The Salk Institute Biotechnology/Industrial Associates, Inc. Mixed feed recombinant yeast fermentation
EP0510693A2 (en) * 1991-04-25 1992-10-28 Research Corporation Technologies, Inc. Expression of human serum albumin in pichia pastoris
WO1995021928A1 (en) * 1994-02-15 1995-08-17 Smithkline Beecham Biologicals (S.A.) Process for the production of a heterologous protein from a methylotrophic yeast
GB2292382A (en) * 1994-08-17 1996-02-21 Univ Rockefeller Obesity(OB) polypeptides
WO1998002565A1 (en) * 1996-07-17 1998-01-22 Zymogenetics, Inc. TRANSFORMATION OF $i(PICHIA METHANOLICA)
US5854039A (en) * 1996-07-17 1998-12-29 Zymogenetics, Inc. Transformation of pichia methanolica

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990003431A1 (en) * 1988-09-26 1990-04-05 The Salk Institute Biotechnology/Industrial Associates, Inc. Mixed feed recombinant yeast fermentation
EP0510693A2 (en) * 1991-04-25 1992-10-28 Research Corporation Technologies, Inc. Expression of human serum albumin in pichia pastoris
WO1995021928A1 (en) * 1994-02-15 1995-08-17 Smithkline Beecham Biologicals (S.A.) Process for the production of a heterologous protein from a methylotrophic yeast
GB2292382A (en) * 1994-08-17 1996-02-21 Univ Rockefeller Obesity(OB) polypeptides
WO1998002565A1 (en) * 1996-07-17 1998-01-22 Zymogenetics, Inc. TRANSFORMATION OF $i(PICHIA METHANOLICA)
US5854039A (en) * 1996-07-17 1998-12-29 Zymogenetics, Inc. Transformation of pichia methanolica

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
C.K. RAYMOND: "Recombinant protein expression in Pichia methanolica", GENE EXPRESSION SYSTEMS: USING NATURE FOR THE ART OF EXPRESSION, 16 November 1998 (1998-11-16), J.M. Fernandez and J.P. Hoeffler (Eds).1999 Academic press, INC., pages 193 - 209, XP000912263 *
J M CREGG ET AL: "Recent Advances in the expression of foreign genes in Pichia pastoris", BIO/TECHNOLOGY,US,NATURE PUBLISHING CO. NEW YORK, vol. 11, no. 11, August 1993 (1993-08-01), pages 905 - 910, XP002100307, ISSN: 0733-222X *
RAYMOND C K ET AL: "Development of the methylotrophic yeast Pichia methanolica for the expression of the 65 kilodalton isoform of human glutamate decarboxylase", YEAST,GB,CHICHESTER, SUSSEX, vol. 14, no. 1, 1 January 1998 (1998-01-01), pages 11 - 23, XP002090804, ISSN: 0749-503X *
YIYING ZHANG ET AL: "POSITIONAL CLONING OF THE MOUSE OBESE GENE AND ITS HUMAN HOMOLOGUE", NATURE,GB,MACMILLAN JOURNALS LTD. LONDON, vol. 372, no. 6505, 1 December 1994 (1994-12-01), pages 425 - 432, XP000602062, ISSN: 0028-0836 *
ZAMOST B L ET AL: "Production of recombinant leptin using a new yeast expression system: Pichia methanolica.", ABSTRACTS OF PAPERS AMERICAN CHEMICAL SOCIETY, vol. 217, no. 1-2, 1999, 217th National Meeting of the American Chemical Society;Anaheim, California, USA; March 21-25, 1999, pages BIOT 075, XP000915144, ISSN: 0065-7727 *

Also Published As

Publication number Publication date
WO2000056903A2 (en) 2000-09-28
AU3910000A (en) 2000-10-09

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