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WO2000054019A1 - Dispositif d'echantillonage de liquides - Google Patents

Dispositif d'echantillonage de liquides Download PDF

Info

Publication number
WO2000054019A1
WO2000054019A1 PCT/AU2000/000182 AU0000182W WO0054019A1 WO 2000054019 A1 WO2000054019 A1 WO 2000054019A1 AU 0000182 W AU0000182 W AU 0000182W WO 0054019 A1 WO0054019 A1 WO 0054019A1
Authority
WO
WIPO (PCT)
Prior art keywords
absorbent material
sides
secured
free end
corresponding end
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/AU2000/000182
Other languages
English (en)
Inventor
Warren Ward
Rosemary Sutton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organization CSIRO
Australian Wool Research and Promotion Organization AWRAP
Original Assignee
Commonwealth Scientific and Industrial Research Organization CSIRO
Australian Wool Research and Promotion Organization AWRAP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO, Australian Wool Research and Promotion Organization AWRAP filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
Priority to GB0121843A priority Critical patent/GB2363198B/en
Priority to NZ514032A priority patent/NZ514032A/en
Priority to AU31346/00A priority patent/AU756329B2/en
Publication of WO2000054019A1 publication Critical patent/WO2000054019A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
    • B01L3/5055—Hinged, e.g. opposable surfaces

Definitions

  • the present invention relates to a device for sampling liquids for subsequent testing.
  • a device for sampling liquids such as solutions, suspensions and slurries, in which a solid is dispersed in a liquid.
  • the invention has particular relevance to the collection of blood samples for subsequent testing.
  • the conventional method for DNA pedigreeing of sheep consists of taking a 5 to 10 ml blood sample from the jugular vein of a sheep using a vacutainer.
  • a vacutainer is a needle attached to a syringe and a vacuum tube. It is a device that requires experience in its use. Normally a veterinary surgeon is required to take a blood sample with a vacutainer.
  • conventional extraction of DNA from whole blood requires expensive reagents and several centrifugation and transfer steps which may take 10 hours of labour over a 3 day period in order to process 100 samples.
  • the conventional extraction technique involves a number of transfer steps, inherently involving the risk of sample mix up.
  • An object of the present invention is to provide a simple method of collecting a sample that can be used in a PCR thermal cycler. Accordingly, the present invention provides a device for sampling a liquid for subsequent testing, the device including a first side, a second side secured to the first side at a corresponding end of each side, and a piece of absorbent material secured between each side, the first and second sides having fold lines adjacent to the corresponding end of each side so that each side may be folded back to an open position to form a handle and expose the absorbent material, and returned to a closed position in which the absorbent material is substantially enclosed by the first and second sides.
  • the absorbent material is paper of a type similar to that known as blotting paper. Any other inert absorbent material may also be used.
  • Each side may be formed from a flat sheet of material, such as cardboard.
  • the first side may be secured to the second side by means of a staple, in which case the absorbent material may also be secured between the two sides by means of the staple.
  • the absorbent material includes one or more tips that are so shaped that they are capable of fitting neatly within a PCR tube, thereby maximising the surface area exposed to elution by a suitable solvent.
  • Either the first or second side may be provided with securing means so that the free end of one can be secured to the free end of the other when the device is in the closed position.
  • the securing means may comprise a partially cut out tab which can be folded back by elastic deformation so that it returns to its original position and secures the free end of the side to which it is not attached.
  • Figure 1 is a perspective view of a device according to the invention, depicting it in a partially open position
  • Figure 2 is a perspective view of the device in the closed position.
  • Figure 1 depicts a sampling device comprising a first side 1 and a second side 2 having a piece of absorbent material 3 secured therebetween by means of a staple 4.
  • the two sides 1 and 2 are formed integrally from a sheet of cardboard.
  • the cardboard has been provided with fold lines on sides 1 and 2, one of which 5 is shown in Figures 1 and 2.
  • the absorbent material has an exposed end comprising one or more tips 6 that are so shaped that when cut off at the base thereof, they fit tip-first, neatly into a PCR tube.
  • Side 2 is provided with a triangular tab 7, two sides of which are cut out and the remaining side 8 of which remains attached to and integral with side 2.
  • side 1 and side 2 are folded back by rotating them about fold lines 5 to a fully open position in which sides 1 and 2 are parallel to each other, and can be held as a handle and the tips 6 of the absorbent material 3 are fully exposed. Whilst held in the open position by the handle formed by sides 1 and 2, a blood sample can be taken by squeezing the ear of the animal to be tested, nicking the ear with a lancet and absorbing blood into the tips 6 of the absorbent material 3. Having taken a sample, the sides 1 and 2 may be returned to the closed position by rotating them back to that position about fold lines 5. Once in the closed position, the tab 7 can be exposed and its tip allowed to elastically close back over the free end 9 of side 1.
  • the name or some other means of identification of the animal may be written on the card, and the card placed in an airtight container with other samples and desiccant and sent to a laboratory for testing.
  • the device In the laboratory the device is opened and the tips 6 cut off and placed tip-first into the next available well of a standard 96 well tray of PCR tubes.
  • the animal identification is recorded on a computer (or manual sheet) against the position coordinates and tray number for sample tracking.
  • the absorbent tips 6 placed in each tube can be efficiently washed and DNA extracted.
  • the process steps involved in pedigreeing involve transferring eluted samples to a second type of 96 well tray, but the sample coordinates can be preserved and a multi-channel pipette or robot-operated pipette can be used to minimise sample mix up. Normally sufficient DNA is extracted from one tip to pedigree an animal for 15 polymorphic markers.
  • sampling device has many other applications, including anonymous testing for antibodies associated with the virus that produces human immune deficiency. It can be used also for blood enzyme tests which are not affected by drying the blood. For example, the three enzyme assays for genetic disease carried out on blood dried on to Guthrie cards taken from most newborn babies born in hospitals in Australia. The device has many other applications, including testing for blood-borne antibodies or host or foreign DNA, associated with disease in animals or humans.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un dispositif d'échantillonnage de liquides, en particulier de sang. Ce dispositif comprend un premier côté (1), un second côté (2) fixé au premier côté, à une extrémité correspondant à chaque côté, et une pièce de matériau absorbant (3) fixée entre chaque côté. Les premier et second côtés présentent des lignes de pliures (5) adjacentes à l'extrémité correspondant à chaque côté, de façon à ce que chacun puisse être déplié en une position ouverte afin de former une poignée et exposer le matériau absorbant. Le dispositif peut être alors replacé en position fermée. Dans cette position, le matériau absorbant est sensiblement enfermé entre les premier et second côtés.
PCT/AU2000/000182 1999-03-10 2000-03-10 Dispositif d'echantillonage de liquides Ceased WO2000054019A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
GB0121843A GB2363198B (en) 1999-03-10 2000-03-10 Device for sampling liquids
NZ514032A NZ514032A (en) 1999-03-10 2000-03-10 Device for sampling liquids
AU31346/00A AU756329B2 (en) 1999-03-10 2000-03-10 Device for sampling liquids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPQ3648 1999-03-10
AUPQ364899 1999-03-10

Publications (1)

Publication Number Publication Date
WO2000054019A1 true WO2000054019A1 (fr) 2000-09-14

Family

ID=3817799

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2000/000182 Ceased WO2000054019A1 (fr) 1999-03-10 2000-03-10 Dispositif d'echantillonage de liquides

Country Status (4)

Country Link
GB (1) GB2363198B (fr)
NZ (1) NZ514032A (fr)
WO (1) WO2000054019A1 (fr)
ZA (1) ZA200107453B (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003050507A1 (fr) * 2001-12-12 2003-06-19 The Secretary Of State For Defence Dispositif d'echantillonnage
GB2570679A (en) * 2018-02-01 2019-08-07 Ge Healthcare Uk Ltd Improvements in and relating to biological sample receivers

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3572997A (en) * 1968-05-24 1971-03-30 Burk Ind Inc Soil testing kit
US3996006A (en) * 1974-08-16 1976-12-07 Smithkline Corporation Specimen test slide
EP0117689A1 (fr) * 1983-03-01 1984-09-05 Smithkline Diagnostics, Inc. Carte de test ameliorée pour la détection de sang occulte
FR2621690A1 (fr) * 1987-10-09 1989-04-14 Lebosse Jean Louis Dispositif destine a servir de support pour des prelevements

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3572997A (en) * 1968-05-24 1971-03-30 Burk Ind Inc Soil testing kit
US3996006A (en) * 1974-08-16 1976-12-07 Smithkline Corporation Specimen test slide
EP0117689A1 (fr) * 1983-03-01 1984-09-05 Smithkline Diagnostics, Inc. Carte de test ameliorée pour la détection de sang occulte
FR2621690A1 (fr) * 1987-10-09 1989-04-14 Lebosse Jean Louis Dispositif destine a servir de support pour des prelevements

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003050507A1 (fr) * 2001-12-12 2003-06-19 The Secretary Of State For Defence Dispositif d'echantillonnage
AU2002350948B2 (en) * 2001-12-12 2006-06-15 The Secretary Of State For Defence Sampling device
GB2570679A (en) * 2018-02-01 2019-08-07 Ge Healthcare Uk Ltd Improvements in and relating to biological sample receivers
GB2570679B (en) * 2018-02-01 2023-01-11 Qiagen Healthcare Biotechnologies Systems Gmbh A biological sample receiver

Also Published As

Publication number Publication date
GB2363198A (en) 2001-12-12
ZA200107453B (en) 2002-09-10
GB0121843D0 (en) 2001-10-31
GB2363198B (en) 2003-04-23
NZ514032A (en) 2000-03-10

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