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WO2000044891A1 - Encre contenant de l'adn - Google Patents

Encre contenant de l'adn Download PDF

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Publication number
WO2000044891A1
WO2000044891A1 PCT/KR2000/000072 KR0000072W WO0044891A1 WO 2000044891 A1 WO2000044891 A1 WO 2000044891A1 KR 0000072 W KR0000072 W KR 0000072W WO 0044891 A1 WO0044891 A1 WO 0044891A1
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WO
WIPO (PCT)
Prior art keywords
dna
ink
oil
containing ink
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2000/000072
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English (en)
Inventor
Jae-Jong Kim
Soo-Hyun Jeong
Sun-Ho Cha
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genotech Corp
Original Assignee
Genotech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genotech Corp filed Critical Genotech Corp
Priority to AU24633/00A priority Critical patent/AU2463300A/en
Publication of WO2000044891A1 publication Critical patent/WO2000044891A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D11/00—Inks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Definitions

  • the present invention relates to DNA-containing ink, more specifically, to DNA-containing ink which comprises DNA amplified by polymerase chain reaction and water- or oil-soluble ink and a method for identifying DNA from goods printed with the DNA-containing ink.
  • PCR Polymerase chain reaction
  • U.S.Pat. No. 5,139,812 discloses the DNA hybridization technique for secret keeping, verification of the identity and authenticity of goods, which comprises the steps cf preparing a solution which contains a large amount of single strand of DNA to be used in printed matters, and detecting the said single stranded DNA by hybridization.
  • U.S.Pat. Nos. 5,599,578 and 3,886,083 disclose ink for verifying the identity or authenticity of goods. However, it has also revealed critical demerits that: it is not detectable under visible light, since they employed UV dye, IR dye or fluorescent pigment which are visible only under UV or IR; and, they do not teach a method for verifying the identity of an individual.
  • the present inventors have made an effort to establish a method for verifying the identity or authenticity of an individual, and found that DNA of an individual can be identified by PCR-amplification of DNA from the goods printed with ink comprising DNA of an individual and water- or oil-soluble ink.
  • a primary object of the present invention is, therefore, to provide ink that comprises DNA of an individual and water- or oil-soluble ink.
  • the other object of the invention is to provide a method for identifying DNA by PCR amplification of DNA using a template of the DNA from goods printed with the DNA-containing ink.
  • Figure 1 is a photograph showing agarose gel eletrophoresis pattern of beta-actin DNA amplified by PCR using conventional primers, amine primer and MMT (mono- methoxytrityl) -amine primer, repectively.
  • Figure 2 is a photograph showing agarose gel electrophoresis pattern for comparing identification efficiency of PCR- amplified DNA by PCR using conventional primer, amine primer and MMT (mono- methoxytrityl) -amine primer, respectively.
  • Figure 3 is a photograph showing agarose gel electrophoresis pattern for identifying DNA purified from water-soluble ink for fountain pen by PCR technique.
  • Figure 4 is a photograph showing agarose gel electrophoresis pattern for identifying DNA purified from water-soluble ink for stamp by PCR technique.
  • Figure 5 is a photograph showing agarose gel electrophoresis pattern for identifying DNA purified from water-soluble DNA- containing ink dropped in copying paper.
  • Figure 6 is a photograph showing agarose gel electrophoresis pattern for identifying DNA purified from various kinds of water- soluble DNA-containing ink by PCR.
  • Figure 7 is a photograph showing agarose gel electrophoresis pattern for identifying DNA purified from oil DNA-containing ink.
  • Figure 8 is a photograph showing agarose gel electrophoresis pattern of PCR results of oil-soluble ink containing DNA and olive oil confirmation of homogenity.
  • Figure 9 is a photograph showing agarose gel electrophoresis pattern for identifying DNA purified from oil-soluble DNA- containing ink dropped in copying paper by PCR technique.
  • Figure 10 is a photograph showing agarose gel electrophoresis pattern for identifying
  • DNA-containing ink of the present invention is prepared to comprise 10 2 ⁇ 10 7 copy of amplified DNA in 1 ⁇ of the ink. All kinds of commercially available water- or oil-soluble ink may be employed in the DNA-containing ink.
  • primers used in the amplification of the DNA include: a primer free of any residue connected to the 5' -end of synthesized oligonucleotide (hereinafter referred to as "conventional primer”); a primer having hexylamine group to the 5' -end to prevent information outflow of amplified DNA by prohibiting DNA contained in the DNA-containing ink from being cloned to cloning vector or expression vector (hereinafter referred to as "amine primer”); and, a primer having monomethoxytrityl (MMT) -hexylamine group to the 5' -end which confers the hydrophobicity of MMT group upon the primer (hereinafter referred to as "MMT-amine primer” ) .
  • the oil-soluble ink is added to a suspension containing oil such as mineral oil, corn oil or olive oil so that the amplified DNA can be mixed uniformly in the oil-soluble ink.
  • PCR technique enables a great amount of DNA- containing ink to be prepared with a small amount of DNA and allows DNA to be identified accurately with a trace amount of DNA-containing ink because a specific region of target DNA can be amplified to maximum of 10 8 times (in case of 30 cycles of PCR: 1.85 J ⁇ ) .
  • approximately l ⁇ 10ng/ ⁇ # of amplified DNA can be obtained using lOOng (about 10 5 copy) of genomic DNA purified from human blood in 30 cycles of PCR which corresponds to 10 12 ⁇ 10 13 copy when the size of DNA is lOObp.
  • At least 10 ⁇ 10 5 copy of target DNA is used to detect the PCR product by agarose gel electrophoresis, and 1-10 copy is used when nested PCR is employed. Accordingly, in case of using 1 ⁇ l of DNA-containing ink, 10 4 ⁇ 10 5 copy of DNA which should be contained in the 1 ⁇ l of DNA-containing ink (1-10 cop / ⁇ Jl DNA-containing ink with nested PCR), indicating that 100-1, 000L of DNA-containing ink can be prepared with 1 ⁇ J, of PCR reaction solution (1, 000,000-10, 000, 000L DNA-containing ink with nested PCR) .
  • DNA-containing ink is employed as means of verifying the identity or authenticity of goods printed with the DNA-containing ink
  • numerous DNA- containing ink should be provided for respective goods and individuals together with corresponding numerous PCR primers.
  • the present inventor overcame the said problem by adding 4-5 kinds of different DNAs into the DNA-containing ink.
  • 200 kinds of DNAs are amplified with 200 kinds of primer pairs
  • 200 C 4 ( 6.4xl0 7 ) or 200 C 5 (2.5xl0 9 ) kinds of DNA-containing ink with different combination can be prepared by employing 4-5 kinds of DNAs in the DNA- containing ink.
  • DNA-containing ink comprising 5 kinds of DNA
  • 1 liter of one billion kinds of different DNA- containing ink can be manufactured by employing 500 ⁇ l of PCR reaction solution prepared with 200 kinds of primer pairs.
  • the DNA-containing ink of the invention can be provided in a variety form, while preventing information overflow by containing 4-5 kinds of DNAs simultaneously and protecting 5' -end of primer.
  • DNA-containing ink of the present invention can be used for verifying the identity of an individual with trace amount of DNA, while preventing characteristic DNA information overflow by the aid of PCR technique which allows specific binding of DNAs.
  • Example 1 Amplification of beta-actin by PCR
  • DNA was amplified by carrying out PCR employing a template of specific region of beta-actin: human genomic DNA was extracted from whole blood with phenol/chloroform and PCR was performed to amplify the specific region of beta-actin by employing primers of SI: 5'- CATGTTTGAGACCTTCAACACCCC-3' (SEQ ID NO:l) and AS1: 5'- GGAGTCCATCACGATGCCAGTGG-3' (SEQ ID NO:2) (amplified product: lOObp) , which may be replaced by amine primer having hydroxylamime group to its 5' -end and MMT-amine primer having MMT-amine group to its 5' -end.
  • the PCR was carried out with 50 ⁇ l of reaction solution containing lxPCR buffer, 250 ⁇ M of each deoxynucleoside triphosphate, 1.0 unit of Taq DNA polymerase, lOOng of genomic DNA and 20pmole of each primer. Reaction solution was heated at 94°C for 5 min, and the cycle of 94°C for 45 sec, 60°C for 45 sec, 72°C for 45sec was repeated 30 times and, finally, the reaction was carried out at 72°C for 5 min. In consequence, desired lOObp fragment was amplified by PCR reaction as shown in Fig. 1.
  • 10 9 copy of DNA was used as template in lanes of 1, 5 and 9; 10 6 copy of DNA, in lanes of 2, 6 and 10; 10 5 copy of DNA, in lanes of 3, 7 and 11; 10 4 copy of DNA, in lanes of 4, 8 and 12; and, lanes 13 and 14 are negative control (an arrow indicates a portion of the amplified product) .
  • DNA-containing ink comprising 10 6 copy DNA per ⁇ l was prepared by adding 0.1fg(10 8 copy) of amplified DNA to lOO ⁇ l of commercially available 3 kinds of water-soluble ink for fountain pen (black, red, blue) and water soluble ink for stamp (red, blue), where conventional primer, amine primer and MMT-amine primer were employed similarly as in Example 1.
  • DNA was purified from the water-soluble DNA- containing ink prepared in Example 2 by using QIAquick PCR purification kit(QIAGEN GmbH, Germany): After 1 ⁇ l, of DNA- containing ink was mixed with 100 ⁇ l, of distilled water, 400 ⁇ l. of PB buffer solution was added and spin-columned. Then, DNA-containing ink was extracted with 100 ⁇ l, of distilled water to purify DNA. Then, 10 ⁇ l, taken from them was applied to PCR under the same condition as in Example 1, and electrophoresed on 2.5% (w/v) agarose gel to confirm whether DNA was reamplified by PCR or not (see: Figs. 3 and 4) .
  • lane 1 is a control of PCR product obtained by using undiluted 10 5 copy of DNA
  • lanes 2-4 represent PCR products obtained by using 1/10 (10 5 copy) of DNA extracted from 1 ⁇ l of 10 6 copy of DNA in distilled water
  • lanes 5-7 in black ink
  • lanes 8-10 in blue ink
  • lanes 8-10 in red ink
  • Lanes 2, 5, 8 and 11 are PCR products obtained with conventional primer
  • lanes 4 7, 12 and 13, with MMT-amine primer are examples of PCR product obtained with conventional primer
  • lanes 3, 6, 9 and 12, with amine primer and, lanes 4, 7, 12 and 13, with MMT-amine primer.
  • lane 1 is a control of PCR product obtained by using undiluted 10 5 copy DNA
  • lane 2-4 represent PCR products obtained by using 1/10 (10 5 copy) of DNA extracted from of 10 6 copy of DNA in distilled water
  • lane 5-7 in blue ink for stamp
  • lane 8-10 in red ink for stamp.
  • Lanes 2, 5 and 8 represent PCR products obtained with conventional primer
  • lanes 4, 7 and 10, with MMT-amine primer an arrow indicates a position of the amplified product
  • DNA was extracted from the printed matter and amplified by PCR, after printing was carried out with water-soluble DNA-containing ink prepared in Example 2: that is, 1 ⁇ l, of each water- soluble DNA-containing ink containing PCR amplified DNA of 10 6 copy DNA/ ⁇ was dropped on copying paper, the ink- dropped region, after 12 hrs, was excised, left in distilled water for 20 min, and DNA was extracted in an analogous manner as in Example 3. Amplified DNAs were detected in all cases with conventional primer, amine primer and MMT-primer by PCR with extracted DNA as template (_____: Fig. 5).
  • lane 1 shows a PCR product obtained by employing 10" copy of DNA solution
  • lanes 2-4 are PCR products obtained by using 1/10 (10 5 copy) of DNA extracted from 1 ⁇ l of 10 e copy/ ⁇ l, of DNA to black ink
  • lanes 5-7 in blue ink
  • lanes 8-10 in red ink
  • lanes 11-13 to blue ink for stamp
  • lanes 14-16 to red ink for stamp.
  • Lanes 2, 5, 8, 11 and 14 represent PCR products with conventional primer; lanes 3, 6, 9, 12 and 15, with amine primer; and, lanes 4, 7, 10, 13 and 16, with MMT-amine primer (an arrow indicates a position of the amplified product) .
  • the amount of PCR-amplified DNA tends to decrease in the order of conventional primer, amine primer and MMT-amine primer as in Example 3, and four kinds of ink except black ink does not show any apparent difference compared with control.
  • Example 5 Recovery and identification of DNA in case of mixing many kinds of DNAs
  • DNA-containing ink comprising 5 kinds of different DNAs corresponding to many regions of beta-actin was prepared, and DNA was recovered from the said DNA-containing ink and amplified by PCR.
  • SI primer and AS1 primer employed in Example 1 were commonly used, SI primer was used as sense primer, and AS2 : 5'- CCGTCACCGGAGTCCATCACGATC-3' (SEQ ID NO: 3) (amplified product: 108bp) , AS3: 5' -GGGTGACCCCGTCACCGGAGTC-3' (SEQ ID NO: 4) (amplified product: 116bp) , AS4 : 5'- GGCACAGTGTGGGTGACCCCGTC-3' (SEQ ID NO: 5) (amplified product: 126bp), AS5: 5' -AGGGCATACCCCTCGTAGATGGGCAC-3' (SEQ ID NO: 6) (amplified product: 147bp) were additionally used as antisense primer.
  • PCR was carried out by employing a template of beta-actin DNA, and DNA- containing ink was prepared by mixing 0.2 ⁇ l, of amplified DNA(10 e copy DNA) and 100 ⁇ l of blue ink for stamp. DNA was extracted from 1 ⁇ l of the ink by phenol/chloroform extraction, and PCR with the said 5 kinds of primer pairs revealed that 5 kinds of PCR product were observed to be amplified specifically.
  • lanes 1 to 5 are control
  • PCR products using amplified 10 6 copy of undiluted DNA solution and lanes 6 to 10 are PCR products using amplified DNA extracted from DNA-containing blue ink.
  • S1/AS1 primer pairs were used in lanes 1 and 6; S1/AS2, in lanes 2 and 7; S1/AS3, in lanes 3 and 8; S1/AS4, in lanes 4 and 9; and, S1/AS5, in lanes 5 and 10, respectively. From the results, it was clearly demonstrated that individual-specific DNA identification can be realized by employing the DNA-containing ink comprising many kinds of DNAs.
  • Example 6 Preparation of oil-soluble DNA-containing ink
  • Oil-soluble DNA-containing ink was prepared to comprise 10 c copy DNA by employing commercially available red oil-soluble ink for print. Before adding DNA to oil- soluble ink, 10 ⁇ l of mineral oil, corn oil, or olive oil was added to 1 //-C ( 0. Ifg) of DNA amplified with conventional primer, amine primer and MMT-primer of Example 1, respectively. And, it was subjected to a vortex mixer to prepare a suspension which allows uniform mixing of hydrophilic PCR reaction solution in oil-soluble ink, then, oil-soluble DNA-containing ink was prepared by adding 100 ,ul, of oil-soluble red ink and mixing with the said suspension. Also, oil-soluble DNA-containing ink comprising 1 ⁇ l of undiluted DNA solution and 100 ⁇ l of oil- soluble ink was prepared to be used as control for DNA identification.
  • DNA was purified from the oil-soluble DNA-containing ink prepared in Example 6 and used as a template for PCR: 1 ⁇ l of oil-soluble DNA-containing ink was added to 600 ⁇ l, of phenol/chloroform (1 : 1, v/v) , dissolved and mixed with 100 ⁇ l of distilled water. Then, centrifugation was performed at 13,000rpm for 10 min, 100 ⁇ l of upper liquid layer was purified by using QIAquick PCR purification kit. The amount corresponding to 10 5 copy was taken, amplified by PCR in an analogous manner as in Example 1, and electrophoresed on 2.5% (w/v) agarose gel to confirm whether DNA was amplified again or not (see : Fig. 7) .
  • lanes 1 to 3 are PCR products obtained by using 10 5 copy of undiluted DNA solution
  • lines 4-6 represent PCR products obtained by using 1/10 (10 5 copy) of DNA extracted from 1 ⁇ l of 10 e copy//? of DNA in distilled water
  • lanes 10 to 12, in corn oil and, lanes 13 to 15, in olive oil
  • Lanes 1, 4, 7, 10 and 13 represent PCR products obtained with conventional primer; lanes 2, 5, 8, 11 and 14, amine primer; and, lanes 3, 6, 9, 12 and 15, MMT-amine primer (an arrow indicates a position of the amplified product) .
  • MMT-amine primer an arrow indicates a position of the amplified product
  • oil-soluble DNA- containing ink became more effective by adding oil-soluble ink to a suspension containing DNA and oil, since the undiluted DNA mixed with oil-soluble ink directly (lanes 4- 6) shows a trace amount of amplified DNA.
  • the recovery rate of oil-soluble DNA-containing ink was 20-50% which is lower than water-soluble DNA-containing ink.
  • MMT-amine primer was used to mix homogeneously in preparation of oil-soluble DNA-containing ink, low efficiency in PCR amplification appeared similarly as in the water-soluble DNA-containing ink.
  • oil-soluble DNA-containing ink was prepared by employing olive oil to have a final concentration of 10 6 copy ONA/ ⁇ JL , 10 times higher than that of the above oil-soluble DNA-containing ink, DNA was purified and amplified by PCR technique. As shown in Fig. 8, identical result was obtained, while the recovery rate was relatively low.
  • lanes 1-4 represent PCR products amplified with conventional primer; lanes 5-8, with amine primer; lanes 9-12, with MMT-amine primer, where lanes 1, 5 and 9 reveal the controls of PCR products obtained by using 10 6 copy of undiluted DNA solution (an arrow indicates a position of the amplified product) .
  • the rate of DNA amplification with MMT-amine primer was relatively low, since the recovery and identification of DNA were carried out by way of many purification steps.
  • undiluted DNA solution can be mixed uniformly in oil- soluble ink by the addition of mineral oil; and, oil- soluble DNA-containing ink can be prepared effectively by using 10-100 times of more DNA used in water-soluble ink under a consideration that its recovery rate is lower than water-soluble ink.
  • Example 8 Recovery and identification of DNA from printed matter with oil-soluble DNA-containing ink
  • lanes 1- 3 represent controls of PCR products obtained by using 10 D copy of undiluted DNA solution directly in PCR
  • lanes 4-6 PCR products using oil-soluble DNA-containing ink with mineral oil
  • lanes 7-9 corn oil
  • lanes 10-12 olive oil.
  • Lanes 1, 4, 7 and 10 represent PCR-amplified DNA with conventional primer
  • lanes 2, 5, 8 and 11 with amine primer and, lanes 3, 6, 9 and 12, MMT-amine primer (an arrow indicates a position of the amplified product).
  • lanes 1-3 are controls of PCR products obtained by using 10 5 copy of undiluted DNA solution
  • lanes 4-6 represent PCR products using oil-soluble DNA-containing ink with mineral oil
  • lanes 7-9 corn oil
  • lanes 10-12 olive oil
  • Lanes 1, 4, 7 and 10 are PCR-amplified DNA with conventional primer; lanes 2, 5, 8 and 11, with amine primer; and, lanes 3, 6, 9 and 12, with MMT-amine primer (an arrow indicates a position of the amplified product) .
  • oil-soluble DNA-containing ink recovery rate of DNA printed in OHP film was relatively higher than that of paper, though there was a little differences depending on the kinds of oil. Therefore, oil-soluble DNA-containing ink comprising appropriate amount of DNA and oil can be prepared under a consideration of recovery rate of DNA depending on the kinds of copying paper.
  • the present invention provides DNA-containing ink which comprises DNA amplified by polymerase chain reaction and water- or oil-soluble ink and a method for identifying DNA from goods printed with the DNA-containing ink.
  • DNA-containing ink of the invention printed matters and various kinds of products containing characteristic DNA information of an individual can be prepared, which can be applied in secret-keeping, identity confirmation, verification of authentic goods and manufacture of scarce goods.
  • the DNA-containing ink is available in product advertisement in the form of printed goods with the DNA-containing ink comprising prominent indivisual's DNA, since authenticity of the goods printed with the DNA- containing ink can be verified accurately and easily.
  • DNA-containing ink of the invention can be prepared with a small amount of genomic DNA in a simple manner, which provides advantages of low-priced unit cost, simple manufacturing process, and unnecessity of excessive facility for mass production.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne de l'encre contenant de l'ADN qui renferme de l'ADN amplifié par réaction en chaîne de la polymérase, de l'encre soluble dans l'eau ou dans l'huile, et un procédé permettant d'identifier l'ADN à partir de produits imprimés au moyen de ladite encre contenant de l'ADN. Cette encre est préparée de manière à renfermer plusieurs copies de l'ADN amplifié. Elle peut être utilisée pour vérifier l'identité d'un individu au moyen d'une quantité de traces d'ADN, tout en empêchant le débordement des informations caractéristiques de l'ADN à l'aide de la technique PCR qui permet une liaison spécifique des ADN.
PCT/KR2000/000072 1999-01-30 2000-01-28 Encre contenant de l'adn Ceased WO2000044891A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU24633/00A AU2463300A (en) 1999-01-30 2000-01-28 Dna-containing ink

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1019990003017A KR100294374B1 (ko) 1999-01-30 1999-01-30 Ｄｎａ 함유 잉크 및 그의 제조방법
KR1999/3017 1999-01-30

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WO2000044891A1 true WO2000044891A1 (fr) 2000-08-03

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PCT/KR2000/000072 Ceased WO2000044891A1 (fr) 1999-01-30 2000-01-28 Encre contenant de l'adn

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KR (1) KR100294374B1 (fr)
AU (1) AU2463300A (fr)
WO (1) WO2000044891A1 (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003038000A1 (fr) * 2001-11-02 2003-05-08 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Solution de marquage pour l'identification infalsifiable d'un objet de valeur, marquage realise a l'aide de cette solution de marquage et procede de marquage d'un objet de valeur
EP1237327A3 (fr) * 2001-03-01 2003-07-02 NTT Data Technology Corporation Procédé et dispositif pour l'authentification individuelle et la signature numérique utilisant un dispositif comportant une marque d'identification basée sur un code ADN
GB2390055A (en) * 2002-03-22 2003-12-31 Cypher Science Internat Ltd Applying and analysing a nucleic acid marker.
WO2006120653A2 (fr) 2005-05-13 2006-11-16 Biolab S.P.A. Traceur d'aliments naturels
US20110229881A1 (en) * 2008-09-11 2011-09-22 Nagahama Bio-Laboratory Incorporated Dna-containing ink composition
WO2012023911A1 (fr) * 2010-08-18 2012-02-23 Yilmaz Dal Dispositif automatique déterminant des couleurs caractéristiques et produisant des mélanges
WO2012055107A1 (fr) * 2010-10-28 2012-05-03 深圳智慧天使投资有限公司 Composition d'encre en émulsion eau dans huile et son utilisation
WO2016134680A1 (fr) 2015-02-26 2016-09-01 Univerzita Palackeho V Olomouci Système et procédé pour la vérification de l'authenticité de produits

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010018878A (ko) * 1999-08-23 2001-03-15 김정호 Ｄｎａ구조를 분석하여 그 분석한 ｄｎａ구조를 물품에 형상화하는 방법 및 그 물품
KR20050120549A (ko) * 2004-07-05 2005-12-22 이학록 졸-겔법을 이용한 ｄｎａ 함유 제품의 제조방법

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US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5139812A (en) * 1989-07-07 1992-08-18 Bioprobe Systems Method and apparatus for high security crypto-marking for protecting valuable objects

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683202B1 (fr) * 1985-03-28 1990-11-27 Cetus Corp
US5139812A (en) * 1989-07-07 1992-08-18 Bioprobe Systems Method and apparatus for high security crypto-marking for protecting valuable objects

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1237327A3 (fr) * 2001-03-01 2003-07-02 NTT Data Technology Corporation Procédé et dispositif pour l'authentification individuelle et la signature numérique utilisant un dispositif comportant une marque d'identification basée sur un code ADN
EP1515268A3 (fr) * 2001-03-01 2007-12-12 NTT Data Technology Corporation Procédé et dispositif pour l'authentification individuelle et la signature numérique utilisant un dispositif comportant une marque d'identification basée sur un code ADN
WO2003038000A1 (fr) * 2001-11-02 2003-05-08 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Solution de marquage pour l'identification infalsifiable d'un objet de valeur, marquage realise a l'aide de cette solution de marquage et procede de marquage d'un objet de valeur
US8783194B2 (en) 2002-03-22 2014-07-22 Trace Tag International Limited Marking apparatus for nucleic acid marking of items
GB2390055A (en) * 2002-03-22 2003-12-31 Cypher Science Internat Ltd Applying and analysing a nucleic acid marker.
GB2390055B (en) * 2002-03-22 2005-09-07 Cypher Science Internat Ltd A marking apparatus
WO2006120653A2 (fr) 2005-05-13 2006-11-16 Biolab S.P.A. Traceur d'aliments naturels
US20110229881A1 (en) * 2008-09-11 2011-09-22 Nagahama Bio-Laboratory Incorporated Dna-containing ink composition
US9062218B2 (en) * 2008-09-11 2015-06-23 Nagahama Bio-Laboratory Incorporated DNA-containing ink composition
WO2012023911A1 (fr) * 2010-08-18 2012-02-23 Yilmaz Dal Dispositif automatique déterminant des couleurs caractéristiques et produisant des mélanges
WO2012055107A1 (fr) * 2010-10-28 2012-05-03 深圳智慧天使投资有限公司 Composition d'encre en émulsion eau dans huile et son utilisation
CN103328588A (zh) * 2010-10-28 2013-09-25 深圳智慧天使投资有限公司 一种w/o乳液油墨组合物及其应用
WO2016134680A1 (fr) 2015-02-26 2016-09-01 Univerzita Palackeho V Olomouci Système et procédé pour la vérification de l'authenticité de produits

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