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WO2000040603A3 - Production of recombinant monellin using methylotrophic yeast expression system - Google Patents

Production of recombinant monellin using methylotrophic yeast expression system Download PDF

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Publication number
WO2000040603A3
WO2000040603A3 PCT/US1999/029213 US9929213W WO0040603A3 WO 2000040603 A3 WO2000040603 A3 WO 2000040603A3 US 9929213 W US9929213 W US 9929213W WO 0040603 A3 WO0040603 A3 WO 0040603A3
Authority
WO
WIPO (PCT)
Prior art keywords
monellin
protein
nucleic acid
production
expression system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/029213
Other languages
French (fr)
Other versions
WO2000040603A2 (en
Inventor
Lingxun Duan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GenWay Biotech Inc
Original Assignee
GenWay Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GenWay Biotech Inc filed Critical GenWay Biotech Inc
Priority to JP2000592311A priority Critical patent/JP2002534074A/en
Priority to CA002364005A priority patent/CA2364005A1/en
Priority to EP99965192A priority patent/EP1141304A2/en
Priority to KR1020017008413A priority patent/KR20020008819A/en
Priority to AU31161/00A priority patent/AU3116100A/en
Priority to US09/869,445 priority patent/US6780615B1/en
Priority to BR9916724-7A priority patent/BR9916724A/en
Priority to IL14377199A priority patent/IL143771A0/en
Publication of WO2000040603A2 publication Critical patent/WO2000040603A2/en
Publication of WO2000040603A3 publication Critical patent/WO2000040603A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a single-chain monellin-like protein which is stable and which is at least 100-fold sweet as compared to sucrose on the weight basis. The present invention also relates to a nucleic acid encoding said monellin-like protein. Preferably, the nucleic acid further comprises a promoter and a signal sequence for directing expression and secretion of the encoded monellin-like protein in the methylothrophic yeast Pichia pastoris. The present invention further relates to a recombinant Pichia pastoris cell containing the nucleic acid encoding the monellin-like protein, a process for producing the monellin-like protein from the recombinant Pichia pastoris and product of the process.
PCT/US1999/029213 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system Ceased WO2000040603A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP2000592311A JP2002534074A (en) 1998-12-31 1999-12-09 Production of recombinant monellin using a methylotrophic yeast expression system
CA002364005A CA2364005A1 (en) 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system
EP99965192A EP1141304A2 (en) 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system
KR1020017008413A KR20020008819A (en) 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system
AU31161/00A AU3116100A (en) 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system
US09/869,445 US6780615B1 (en) 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system
BR9916724-7A BR9916724A (en) 1998-12-31 1999-12-09 Production of recombinant monelin using expression system in methylotrophic yeasts
IL14377199A IL143771A0 (en) 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11452998P 1998-12-31 1998-12-31
US60/114,529 1998-12-31

Publications (2)

Publication Number Publication Date
WO2000040603A2 WO2000040603A2 (en) 2000-07-13
WO2000040603A3 true WO2000040603A3 (en) 2000-09-21

Family

ID=22355787

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/029213 Ceased WO2000040603A2 (en) 1998-12-31 1999-12-09 Production of recombinant monellin using methylotrophic yeast expression system

Country Status (9)

Country Link
EP (1) EP1141304A2 (en)
JP (1) JP2002534074A (en)
KR (1) KR20020008819A (en)
CN (1) CN1332802A (en)
AU (1) AU3116100A (en)
BR (1) BR9916724A (en)
CA (1) CA2364005A1 (en)
IL (1) IL143771A0 (en)
WO (1) WO2000040603A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023222111A1 (en) * 2022-05-20 2023-11-23 南京百斯杰生物工程有限公司 Sweet protein monellin mutant having high sweetness and method for preparing same

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3998798A (en) * 1972-12-22 1976-12-21 The Trustees Of The University Of Pennsylvania Monellin, a sweet polypeptide derived from fruit of dioscoreophyllum cumminsii
WO1988010265A1 (en) * 1987-06-19 1988-12-29 The Regents Of The University Of California A new class of low calorie protein sweeteners
EP0821055A2 (en) * 1996-07-25 1998-01-28 INTERNATIONAL FLAVORS & FRAGRANCES INC. Use of monellin in enhancing the flavour of beverages and foodstuffs

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3998798A (en) * 1972-12-22 1976-12-21 The Trustees Of The University Of Pennsylvania Monellin, a sweet polypeptide derived from fruit of dioscoreophyllum cumminsii
WO1988010265A1 (en) * 1987-06-19 1988-12-29 The Regents Of The University Of California A new class of low calorie protein sweeteners
EP0821055A2 (en) * 1996-07-25 1998-01-28 INTERNATIONAL FLAVORS & FRAGRANCES INC. Use of monellin in enhancing the flavour of beverages and foodstuffs

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CREGG ET AL: "Recent advances in the expression of foreign genes in Pichia pastoris", BIO/TECHNOLOGY, vol. 11, 1993, pages 905 - 910, XP002100307 *
KONDO K ET AL: "High-level expression of a sweet protein, monellin, in the food yeast Candida utilis", NATURE BIOTECHNOLOGY, vol. 15, no. 5, May 1997 (1997-05-01), pages 453 - 457, XP000910200 *
VEDVICK TS: "Gene expression in yeast: Pichia pastoris", CURR. OPIN. BIOTECHNOL., vol. 2, no. 5, October 1991 (1991-10-01), pages 742 - 745, XP000915828 *

Also Published As

Publication number Publication date
JP2002534074A (en) 2002-10-15
CA2364005A1 (en) 2000-07-13
IL143771A0 (en) 2002-04-21
KR20020008819A (en) 2002-01-31
WO2000040603A2 (en) 2000-07-13
CN1332802A (en) 2002-01-23
EP1141304A2 (en) 2001-10-10
AU3116100A (en) 2000-07-24
BR9916724A (en) 2001-09-11

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