[go: up one dir, main page]

WO1999036571A3 - Procede de detection de sequences nucleotidiques - Google Patents

Procede de detection de sequences nucleotidiques Download PDF

Info

Publication number
WO1999036571A3
WO1999036571A3 PCT/EP1999/000161 EP9900161W WO9936571A3 WO 1999036571 A3 WO1999036571 A3 WO 1999036571A3 EP 9900161 W EP9900161 W EP 9900161W WO 9936571 A3 WO9936571 A3 WO 9936571A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
detection
solid support
ligation
molecules
Prior art date
Application number
PCT/EP1999/000161
Other languages
English (en)
Other versions
WO1999036571A2 (fr
Inventor
Holger Klapproth
Hubert S Bernauer
Original Assignee
Biochip Technologies Gmbh
Holger Klapproth
Hubert S Bernauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biochip Technologies Gmbh, Holger Klapproth, Hubert S Bernauer filed Critical Biochip Technologies Gmbh
Priority to EP99907365A priority Critical patent/EP1047794A2/fr
Priority to CA002318371A priority patent/CA2318371A1/fr
Priority to JP2000540272A priority patent/JP2002517981A/ja
Publication of WO1999036571A2 publication Critical patent/WO1999036571A2/fr
Publication of WO1999036571A3 publication Critical patent/WO1999036571A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un nouveau procédé de détection d'un séquence nucléotidique au sein d'une molécule d'acide nucléique. Le procédé de l'invention consiste principalement à combiner la protection de l'acide nucléique, la ligature d'oligonucléotides auxdites molécules d'acide nucléique protégées et l'amplification des produits de la ligature. La détection des produits amplifiés s'effectue avantageusement par la conversion de ces derniers, de sorte qu'ils se présentent sous une forme à simple brin, et par l'hybridation de leur forme à simple brin en un réseau de molécules d'acide nucléique simple brin d'au moins une séquence partiellement déterminée, fixée à un support solide. Le support solide est de préférence une pastille. La détection des molécules hybridées peut s'effectuer selon des procédés classiques. L'invention concerne également un kit pour la mise en oeuvre du procédé de l'invention.
PCT/EP1999/000161 1998-01-13 1999-01-13 Procede de detection de sequences nucleotidiques WO1999036571A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99907365A EP1047794A2 (fr) 1998-01-13 1999-01-13 Procede de detection de sequences nucleotidiques
CA002318371A CA2318371A1 (fr) 1998-01-13 1999-01-13 Procede de detection de sequences nucleotidiques
JP2000540272A JP2002517981A (ja) 1998-01-13 1999-01-13 核酸配列を検出するための方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98100485.6 1998-01-13
EP98100485 1998-01-13

Publications (2)

Publication Number Publication Date
WO1999036571A2 WO1999036571A2 (fr) 1999-07-22
WO1999036571A3 true WO1999036571A3 (fr) 1999-10-21

Family

ID=8231257

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/000161 WO1999036571A2 (fr) 1998-01-13 1999-01-13 Procede de detection de sequences nucleotidiques

Country Status (4)

Country Link
EP (1) EP1047794A2 (fr)
JP (1) JP2002517981A (fr)
CA (1) CA2318371A1 (fr)
WO (1) WO1999036571A2 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8901287B2 (en) 2004-10-20 2014-12-02 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9410146B2 (en) 2009-09-14 2016-08-09 Qiagen Gaithersburg Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US9422593B2 (en) 2010-05-19 2016-08-23 Qiagen Gaithresburg, Inc Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6686151B1 (en) * 1998-02-06 2004-02-03 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US7399589B2 (en) 1998-02-06 2008-07-15 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
WO2000024939A1 (fr) 1998-10-27 2000-05-04 Affymetrix, Inc. Gestion de la complexite et analyse d'adn genomique
CA2377739A1 (fr) 1999-07-02 2001-01-11 Symyx Technologies, Inc. Brosses polymeres destinees a immobiliser des molecules sur une surface ou un substrat, dans lesquelles les polymeres presentent des segments dispersables ou solubles dans l'eau et capteurs y etant lies
EP1111069A1 (fr) * 1999-12-22 2001-06-27 BioChip Technologies GmbH Des acides nucléiques modifiés et leur utilisation
ATE206211T1 (de) * 2000-05-16 2001-10-15 Biochip Technologies Gmbh Linker-system zum aktivieren von oberflächen für die biokonjugation und verfahren zu ihrer verwendung
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
JP2002176985A (ja) * 2000-12-14 2002-06-25 Hitachi Software Eng Co Ltd Ｐｃｒ増幅された塩基配列の検出方法及び検出キット
US7205161B2 (en) 2001-01-10 2007-04-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate having improved stability
US9074244B2 (en) 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
WO2010062546A1 (fr) 2008-10-27 2010-06-03 Qiagen Gaithersburg Inc. Essai de capture d’hybride à résultats rapides sur une plate-forme automatisée
WO2010127228A1 (fr) 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. Procédé d'amplification non ciblée pour la détection de formes d'épissage d'arn dans un échantillon
WO2011094514A1 (fr) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Procédés et compositions pour la purification et l'analyse multiplexée d'acides nucléiques séquence-spécifique
AU2011210748A1 (en) 2010-01-29 2012-08-09 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of HPV in a sample
CN103597095B (zh) 2011-02-24 2016-08-17 奇亚根盖瑟斯堡股份有限公司 用于检测hpv核酸的材料和方法
US9315807B1 (en) 2013-01-26 2016-04-19 New England Biolabs, Inc. Genome selection and conversion method
US10087481B2 (en) 2013-03-19 2018-10-02 New England Biolabs, Inc. Enrichment of target sequences
US9708658B2 (en) 2013-03-19 2017-07-18 New England Biolabs, Inc. Enrichment of target sequences
CN111979303A (zh) * 2020-08-11 2020-11-24 上海奕谱生物科技有限公司 一种核酸检测试剂盒、方法及其应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012695A1 (fr) * 1988-06-17 1989-12-28 Genelabs Incorporated Techniques d'amplification et de soustraction d'adn
EP0372524A2 (fr) * 1988-12-07 1990-06-13 The General Hospital Corporation Procédé pour l'enrichissement et le clonage d'ADN équivalent à une insertion ou une délétion
WO1990009457A2 (fr) * 1989-02-14 1990-08-23 Biogen, Inc. Paires d'amorce d'oligonucleotides pour l'amplification genetique independante de la sequence et leurs procedes d'utilisation
WO1995015974A1 (fr) * 1993-12-06 1995-06-15 Pna Diagnostics A/S Acides nucleiques de protection et procedes d'analyse
WO1996036731A2 (fr) * 1995-05-19 1996-11-21 Trustees Of Boston University Procedes de detection d'acides nucleiques
WO1997019192A1 (fr) * 1995-11-20 1997-05-29 Trustees Of Boston University Procede et sonde servant a detecter une sequence cible d'acide nucleique
WO1997047640A1 (fr) * 1996-06-14 1997-12-18 Sarnoff Corporation Dosages avec protection par une nuclease

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989012695A1 (fr) * 1988-06-17 1989-12-28 Genelabs Incorporated Techniques d'amplification et de soustraction d'adn
EP0372524A2 (fr) * 1988-12-07 1990-06-13 The General Hospital Corporation Procédé pour l'enrichissement et le clonage d'ADN équivalent à une insertion ou une délétion
WO1990009457A2 (fr) * 1989-02-14 1990-08-23 Biogen, Inc. Paires d'amorce d'oligonucleotides pour l'amplification genetique independante de la sequence et leurs procedes d'utilisation
WO1995015974A1 (fr) * 1993-12-06 1995-06-15 Pna Diagnostics A/S Acides nucleiques de protection et procedes d'analyse
WO1996036731A2 (fr) * 1995-05-19 1996-11-21 Trustees Of Boston University Procedes de detection d'acides nucleiques
WO1997019192A1 (fr) * 1995-11-20 1997-05-29 Trustees Of Boston University Procede et sonde servant a detecter une sequence cible d'acide nucleique
WO1997047640A1 (fr) * 1996-06-14 1997-12-18 Sarnoff Corporation Dosages avec protection par une nuclease

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YANG M ET AL: "CLONING DIFFERENTIALLY EXPRESSED GENES BY LINKER CAPTURE SUBTRACTION", ANALYTICAL BIOCHEMISTRY, vol. 237, no. 1, 15 May 1996 (1996-05-15), pages 109 - 114, XP000587696 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8901287B2 (en) 2004-10-20 2014-12-02 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9410146B2 (en) 2009-09-14 2016-08-09 Qiagen Gaithersburg Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US9422593B2 (en) 2010-05-19 2016-08-23 Qiagen Gaithresburg, Inc Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes

Also Published As

Publication number Publication date
WO1999036571A2 (fr) 1999-07-22
EP1047794A2 (fr) 2000-11-02
CA2318371A1 (fr) 1999-07-22
JP2002517981A (ja) 2002-06-25

Similar Documents

Publication Publication Date Title
WO1999036571A3 (fr) Procede de detection de sequences nucleotidiques
CA2365135A1 (fr) Procede d'amplification d'une sequence d'acide nucleique
WO1997018334A3 (fr) Sondes d'acides nucleiques complementaires des acides nucleiques du virus du papillome humain, methodes et preparations associees
WO2002038806A3 (fr) Identification de polymorphismes d'acide nucleique
CA2205353A1 (fr) Reaction d'amplification continue
WO2002061133A3 (fr) Detection d'acide nucleique avec des sondes structurees
WO2001079420A3 (fr) Detection et amplification d'arn par ligature par cible d'adn par une ligase d'arn
WO2001094630A3 (fr) Compositions et methodes d'hybridation d'acides nucleiques a partir de jeux d'echantillons
CA2384838A1 (fr) Procedes et compositions utiles pour l'amplification isothermique lineaire de sequences de polynucleotides
WO2000056927A3 (fr) Detection de differences entre sequences d'acide nucleique faisant appel a la reaction de detection de ligase et a des reseaux adressables
AU2001245945A1 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
AU6846698A (en) Method of nucleic acid amplification
WO2002018641A3 (fr) Detection de polymorphismes dans les genes cyp3a4 et cyp2c9
WO1999055857A3 (fr) Nucleoside triphosphates et leur integration aux ribozymes
WO1996012040A3 (fr) Compositions et procedes de detection de chlamydia trachomatis
WO1999067619A3 (fr) Composes et procedes pour la detection de biomolecules
CA2244891A1 (fr) Detection de differences entre sequences d'acide nucleique faisant appel a la reaction de detection de ligase et a des reseaux adressables
WO2000024931A3 (fr) Detection d'analytes
WO1996022392A3 (fr) Oligonucleotides et sondes d'amplification d'acide nucleique pour borrelia associes a la maladie de lyme
WO2001047944A3 (fr) Acides nucleiques contenant des polymorphismes mononucleotidiques et procedes d'utilisation correspondants
WO2003052133A3 (fr) Analogues oligonucleotidiques comprenant des pseudo-nucleotides intercalants
EP0822256A3 (fr) Polymérase d'ADN thermostable modifiée et composition ayant une polymérase d'ADN pour l'amplification d'acides nucléiques
IL111831A (en) Method of detecting the presence of a nucleic acid in a sample
WO1998048046A3 (fr) Amplification en chaine par polymerase-taqmantm pour detecter des souches pathogenes de e. coli
WO2002034951A3 (fr) Compositions et procedes de detection du virus de l'immunodeficience humaine 2 (vih-2)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): CA JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): CA JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase

Ref document number: 2318371

Country of ref document: CA

Ref country code: CA

Ref document number: 2318371

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 1999907365

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999907365

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 09600137

Country of ref document: US

WWW Wipo information: withdrawn in national office

Ref document number: 1999907365

Country of ref document: EP