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WO1999035285A3 - Hybridization probes which differentiate between the actinomadura madurae group of bacteria and maduromycetes - Google Patents

Hybridization probes which differentiate between the actinomadura madurae group of bacteria and maduromycetes Download PDF

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Publication number
WO1999035285A3
WO1999035285A3 PCT/EP1999/000148 EP9900148W WO9935285A3 WO 1999035285 A3 WO1999035285 A3 WO 1999035285A3 EP 9900148 W EP9900148 W EP 9900148W WO 9935285 A3 WO9935285 A3 WO 9935285A3
Authority
WO
WIPO (PCT)
Prior art keywords
bacteria
maduromycetes
differentiate
group
actinomadura madurae
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1999/000148
Other languages
French (fr)
Other versions
WO1999035285A2 (en
Inventor
Olga Genilloud
Rafael P Mellado
Victor Parro
Vicente Rodriguez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Sharp and Dohme de Espana SA
Original Assignee
Merck Sharp and Dohme de Espana SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp and Dohme de Espana SA filed Critical Merck Sharp and Dohme de Espana SA
Priority to EP99901595A priority Critical patent/EP1044284A2/en
Priority to CA002317897A priority patent/CA2317897A1/en
Publication of WO1999035285A2 publication Critical patent/WO1999035285A2/en
Publication of WO1999035285A3 publication Critical patent/WO1999035285A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The invention provides a method to differentiate between bacteria from the Actinomadura madurae group of bacteria and the Maduromycetes taxa using two nucleic acid probes that are complementary to a conserved region of the Maduromycetes 16S rRNA genes and to a conserved region of the Actinomadura madurae group of bacteria 16S rRNA genes, respectively. The probes permit the rapid detection of DNA coding for the 16S rRNA in a sample differentiating the Actinomadura madurae group of bacteria from Maduromycetes within the Actinomycetales order as well as the A. madurae group of bacteria and Maduromycetes from streptomycetes. The method is accurate and reproducible.
PCT/EP1999/000148 1998-01-08 1999-01-05 Hybridization probes which differentiate between the actinomadura madurae group of bacteria and maduromycetes Ceased WO1999035285A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP99901595A EP1044284A2 (en) 1998-01-08 1999-01-05 Hybridization probes which differentiate between the actinomadura madurae group of bacteria and maduromycetes
CA002317897A CA2317897A1 (en) 1998-01-08 1999-01-05 Hybridization probes which differentiate between the actinomadura madurae group of bacteria and maduromycetes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7079998P 1998-01-08 1998-01-08
US60/070,799 1998-01-08

Publications (2)

Publication Number Publication Date
WO1999035285A2 WO1999035285A2 (en) 1999-07-15
WO1999035285A3 true WO1999035285A3 (en) 1999-10-14

Family

ID=22097462

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/000148 Ceased WO1999035285A2 (en) 1998-01-08 1999-01-05 Hybridization probes which differentiate between the actinomadura madurae group of bacteria and maduromycetes

Country Status (3)

Country Link
EP (1) EP1044284A2 (en)
CA (1) CA2317897A1 (en)
WO (1) WO1999035285A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6821770B1 (en) 1999-05-03 2004-11-23 Gen-Probe Incorporated Polynucleotide matrix-based method of identifying microorganisms
ES2265344T3 (en) 1999-05-03 2007-02-16 Gen-Probe Incorporated POLINUCLEOTIDE PROBES FOR THE DETECTION AND QUANTIFICATION OF ACTINOMYCES.
WO2001023608A2 (en) * 1999-09-27 2001-04-05 Merck Sharp & Dohme De Espana, S.A.E. Hybridization probes which specifically detect strains of the genera microbispora, microtetraspora, nonomuria and planobispora
WO2001023609A2 (en) * 1999-09-27 2001-04-05 Merck Sharp & Dohme De Espana, S.A.E. Hybridization probes which detect bacteria of the genus thermobifida

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988003957A1 (en) * 1986-11-24 1988-06-02 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
WO1995013396A2 (en) * 1993-11-11 1995-05-18 U-Gene Research B.V. A method for identifying microorganisms, and aids useful thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988003957A1 (en) * 1986-11-24 1988-06-02 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
WO1995013396A2 (en) * 1993-11-11 1995-05-18 U-Gene Research B.V. A method for identifying microorganisms, and aids useful thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AMANN R I ET AL: "PHYLOGENETIC IDENTIFICATION AND IN SITU DETECTION OF INDIVIDUAL MICROBIAL CELLS WITHOUT CULTIVATION", MICROBIOLOGICAL REVIEWS, vol. 59, no. 1, 1 March 1995 (1995-03-01), pages 143 - 169, XP002026194 *
STACKEBRANDT ET AL: "Designation of Streptomycete 16S and 23S rRNA-based target regions for oligonucleotide probes", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 57, no. 5, 1 May 1991 (1991-05-01), pages 1468 - 1477, XP002090717, ISSN: 0099-2240 *
WANG ET AL: "Phylogenetic analysis reveals new relationships among members of the genera Microtetraspora and Microbispora", INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, vol. 46, no. 3, 1 July 1996 (1996-07-01), pages 658 - 663, XP002090716 *
WARD-RAINEY ET AL: "The phylogenetic structure of the genus Streptosporangium", SYSTEMATIC AND APPLIED MICROBIOLOGY, vol. 19, 1 March 1996 (1996-03-01), pages 50 - 55, XP002090719, ISSN: 0723-2020 *

Also Published As

Publication number Publication date
EP1044284A2 (en) 2000-10-18
WO1999035285A2 (en) 1999-07-15
CA2317897A1 (en) 1999-07-15

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