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WO1999031262A3 - Needle-free injection of formulated nucleic acid molecules - Google Patents

Needle-free injection of formulated nucleic acid molecules Download PDF

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Publication number
WO1999031262A3
WO1999031262A3 PCT/US1998/026823 US9826823W WO9931262A3 WO 1999031262 A3 WO1999031262 A3 WO 1999031262A3 US 9826823 W US9826823 W US 9826823W WO 9931262 A3 WO9931262 A3 WO 9931262A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
methods
needle
disclosed
Prior art date
Application number
PCT/US1998/026823
Other languages
French (fr)
Other versions
WO1999031262A2 (en
Inventor
Michael Barry
Russ Mumper
Lou Smith
Original Assignee
Valentis Inc
Baylor College Medicine
Michael Barry
Russ Mumper
Lou Smith
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valentis Inc, Baylor College Medicine, Michael Barry, Russ Mumper, Lou Smith filed Critical Valentis Inc
Priority to CA002315256A priority Critical patent/CA2315256A1/en
Priority to AU19229/99A priority patent/AU1922999A/en
Priority to JP2000539160A priority patent/JP2003528024A/en
Priority to EP98964020A priority patent/EP1038016A2/en
Publication of WO1999031262A2 publication Critical patent/WO1999031262A2/en
Publication of WO1999031262A3 publication Critical patent/WO1999031262A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/10—Drugs for disorders of the endocrine system of the posterior pituitary hormones, e.g. oxytocin, ADH

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Oncology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Diabetes (AREA)
  • Communicable Diseases (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)

Abstract

A novel method is provided for delivering nucleic acid molecules through and/or to the skin of mammals by needle-free injection. The method involves the incorporation of formulated nucleic acid molecules with devices for injecting the molecules by air, fluid and/or mechanical pressure. Disclosed are compositions and methods for enhancing the administration to and uptake of nucleic acids in a mammal. The methods disclosed provide an increased immune response by allowing the uptake of formulated nucleic acid molecules by a wide variety of cell types simultaneously. Also disclosed are examples which demonstrate that the combination of formulated nucleic acid molecules and needle-free injection methods results in immune responses which are superior to those obtained by conventional means of delivery. Methods for delivery, as well as methods for formulating nucleic acid molecules with various compounds, such as cationic complexing agents, polymeric and non-polymeric formulations, protective, interactive, non-condensing systems are also disclosed.
PCT/US1998/026823 1997-12-16 1998-12-16 Needle-free injection of formulated nucleic acid molecules WO1999031262A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002315256A CA2315256A1 (en) 1997-12-16 1998-12-16 Needle-free injection of formulated nucleic acid molecules
AU19229/99A AU1922999A (en) 1997-12-16 1998-12-16 Needle-free injection of formulated nucleic acid molecules
JP2000539160A JP2003528024A (en) 1997-12-16 1998-12-16 Needle-free injection of formulated nucleic acid molecules
EP98964020A EP1038016A2 (en) 1997-12-16 1998-12-16 Needle-free injection of formulated nucleic acid molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6975497P 1997-12-16 1997-12-16
US60/069,754 1997-12-16

Publications (2)

Publication Number Publication Date
WO1999031262A2 WO1999031262A2 (en) 1999-06-24
WO1999031262A3 true WO1999031262A3 (en) 1999-09-10

Family

ID=22091009

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/026823 WO1999031262A2 (en) 1997-12-16 1998-12-16 Needle-free injection of formulated nucleic acid molecules

Country Status (5)

Country Link
EP (1) EP1038016A2 (en)
JP (1) JP2003528024A (en)
AU (1) AU1922999A (en)
CA (1) CA2315256A1 (en)
WO (1) WO1999031262A2 (en)

Cited By (2)

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US7517864B2 (en) 2001-05-18 2009-04-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US11932854B2 (en) 2010-10-29 2024-03-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)

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US7070784B1 (en) * 1999-07-20 2006-07-04 Pharmexa A/S Method for down-regulating GDF-8 activity using immunogenic GDF-8 analogues
EP1196617A2 (en) * 1999-07-21 2002-04-17 Immunoporation Ltd. Method for introducing a substance into a cell
US7196066B1 (en) * 1999-11-03 2007-03-27 Powderject Vaccines, Inc. DNA-vaccines based on constructs derived from the genomes of human and animal pathogens
US8273866B2 (en) 2002-02-20 2012-09-25 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SINA)
US8202979B2 (en) 2002-02-20 2012-06-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid
EP1278551A2 (en) 2000-04-21 2003-01-29 Vical Incorporated Compositions and methods for (in vivo) delivery of polynucleotide-based therapeutics
US20050148530A1 (en) 2002-02-20 2005-07-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US9994853B2 (en) 2001-05-18 2018-06-12 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
FR2835749B1 (en) * 2002-02-08 2006-04-14 Inst Nat Sante Rech Med PHARMACEUTICAL COMPOSITION IMPROVING IN VIVO GENE TRANSFER
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
EP1432724A4 (en) 2002-02-20 2006-02-01 Sirna Therapeutics Inc RNA inhibition mediated inhibition of MAP KINASE GENES
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7923547B2 (en) 2002-09-05 2011-04-12 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
WO2005019249A2 (en) 2003-08-15 2005-03-03 University Of Florida Identification of porphyromonas gingivalis virulence polynucleotides for diagnosis, treatment, and monitoring of periodontal diseases
CA2537184A1 (en) * 2003-08-29 2005-03-10 Anges Mg, Inc. Gene therapy for skin disorders using needleless syringes
EP1704250B1 (en) 2003-12-31 2012-09-19 The Penn State Research Foundation Methods for predicting and overcoming resistance to chemotherapy in ovarian cancer
JP2005335054A (en) * 2004-04-27 2005-12-08 Japan Science & Technology Agency Metal nanowire and manufacturing method thereof
US20060040882A1 (en) 2004-05-04 2006-02-23 Lishan Chen Compostions and methods for enhancing delivery of nucleic acids into cells and for modifying expression of target genes in cells
US10508277B2 (en) 2004-05-24 2019-12-17 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
ATE501252T1 (en) 2004-06-22 2011-03-15 Univ Illinois METHOD FOR INHIBITING TUMOR CELL GROWTH USING FOXM1 SIRNS
EP2532378B1 (en) * 2004-12-01 2016-04-27 AcuShot, Inc. Needle-free injector
US20120208824A1 (en) 2006-01-20 2012-08-16 Cell Signaling Technology, Inc. ROS Kinase in Lung Cancer
EP3936621A1 (en) 2006-01-20 2022-01-12 Cell Signaling Technology, Inc. Translocation and mutant ros kinase in human non-small cell lung carcinoma
DK2450437T3 (en) 2006-04-14 2017-09-11 Cell Signaling Technology Inc Gene defects and mutant ALK kinase in human solid tumors
JP2009544281A (en) 2006-07-21 2009-12-17 サイレンス・セラピューティクス・アーゲー Means for inhibiting the expression of protein kinase 3
EP2918598B1 (en) 2007-02-28 2019-01-30 The Govt. Of U.S.A. As Represented By The Secretary Of The Department Of Health And Human Services Brachyury polypeptides and methods for use
DK2494993T3 (en) 2007-05-04 2018-11-12 Marina Biotech Inc Amino acid lipids and uses thereof
DK2203558T3 (en) 2007-10-18 2016-06-27 Cell Signaling Technology Inc TRANSLOCATION AND mutant ROS kinase IN HUMAN NON-small cell lung carcinoma
CA2720363A1 (en) 2008-04-15 2009-10-22 Bira Arya Compositions and methods for delivering inhibitory oligonucleotides
ATE550024T1 (en) 2008-05-30 2012-04-15 Univ Yale TARGETED OLIGONUCLEOTIDE COMPOSITIONS FOR MODIFYING GENE EXPRESSION
CN104382853A (en) 2008-10-16 2015-03-04 玛瑞纳生物技术有限公司 Processes and Compositions for Liposomal and Efficient Delivery of Gene Silencing Therapeutics
EP2881402B1 (en) 2009-02-12 2017-05-10 Cell Signaling Technology, Inc. Mutant ROS expression in human liver cancer
PT2417257E (en) 2009-04-10 2016-06-03 Universität Bern Tricyclo-dna antisense oligonucleotides, compositions, and methods for the treatment of disease
KR101692063B1 (en) 2009-12-09 2017-01-03 닛토덴코 가부시키가이샤 MODULATION OF hsp47 EXPRESSION
WO2011120023A1 (en) 2010-03-26 2011-09-29 Marina Biotech, Inc. Nucleic acid compounds for inhibiting survivin gene expression uses thereof
WO2011133584A2 (en) 2010-04-19 2011-10-27 Marina Biotech, Inc. Nucleic acid compounds for inhibiting hras gene expression and uses thereof
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CA2800065A1 (en) 2010-05-21 2011-11-24 Peptimed, Inc. Reagents and methods for treating cancer
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US20120101108A1 (en) 2010-08-06 2012-04-26 Cell Signaling Technology, Inc. Anaplastic Lymphoma Kinase In Kidney Cancer
US9243246B2 (en) 2010-08-24 2016-01-26 Sirna Therapeutics, Inc. Single-stranded RNAi agents containing an internal, non-nucleic acid spacer
US20140134231A1 (en) 2010-10-11 2014-05-15 Sanford-Burnham Medical Research Institute Mir-211 expression and related pathways in human melanoma
SG10201508981YA (en) 2010-11-01 2015-11-27 Peptimed Inc Compositions Of A Peptide-Based System For Cell-Specific Targeting
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DE102011018499A1 (en) 2011-04-23 2012-10-25 Emc Microcollections Gmbh Topical nanoparticle vaccine for the immune stimulation of dendritic cells in the skin
PL2581448T3 (en) 2011-10-13 2015-08-31 Association Inst De Myologie Tricyclo-phosphorothioate DNA
RU2014125496A (en) 2012-01-12 2016-02-27 Кварк Фармасьютикалс, Инк. COMBINED THERAPY FOR TREATMENT OF HEARING DISORDERS AND EQUILIBRIUM
WO2013158859A1 (en) 2012-04-18 2013-10-24 Cell Signaling Technology, Inc. Egfr and ros1 in cancer
US20150299696A1 (en) 2012-05-02 2015-10-22 Sirna Therapeutics, Inc. SHORT INTERFERING NUCLEIC ACID (siNA) COMPOSITIONS
AU2013315524B2 (en) 2012-09-12 2019-01-31 Quark Pharmaceuticals, Inc. Double-stranded oligonucleotide molecules to p53 and methods of use thereof
HK1210216A1 (en) 2012-09-12 2016-04-15 夸克制药公司 Double-stranded oligonucleotide molecules to p53 and methods of use thereof
AU2013356383B2 (en) 2012-12-06 2017-08-31 Merck Sharp & Dohme Corp. Disulfide masked prodrug compositions and methods
WO2015020960A1 (en) 2013-08-09 2015-02-12 Novartis Ag Novel lncrna polynucleotides
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US12285464B2 (en) 2016-04-18 2025-04-29 The Trustees Of Columbia University In The City Of New York Therapeutic targets involved in the progression of nonalcoholic steatohepatitis (NASH)
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7517864B2 (en) 2001-05-18 2009-04-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US11932854B2 (en) 2010-10-29 2024-03-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)

Also Published As

Publication number Publication date
CA2315256A1 (en) 1999-06-24
JP2003528024A (en) 2003-09-24
WO1999031262A2 (en) 1999-06-24
EP1038016A2 (en) 2000-09-27
AU1922999A (en) 1999-07-05

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