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WO1999027131A3 - Molecule d'acide nucleique liee a la matrice et topologiquement fixee - Google Patents

Molecule d'acide nucleique liee a la matrice et topologiquement fixee Download PDF

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Publication number
WO1999027131A3
WO1999027131A3 PCT/DE1998/003466 DE9803466W WO9927131A3 WO 1999027131 A3 WO1999027131 A3 WO 1999027131A3 DE 9803466 W DE9803466 W DE 9803466W WO 9927131 A3 WO9927131 A3 WO 9927131A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecule
fixed matrix
bonded nucleic
matrix bonded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE1998/003466
Other languages
German (de)
English (en)
Other versions
WO1999027131A2 (fr
Inventor
Claas Junghans
Burghardt Wittig
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to EP98963380A priority Critical patent/EP0975808A2/fr
Publication of WO1999027131A2 publication Critical patent/WO1999027131A2/fr
Publication of WO1999027131A3 publication Critical patent/WO1999027131A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des molécules d'acide nucléique qui sont liées à une surface de telle façon que l'énergie de spire et la superhélicité ne puissent s'en échapper. L'invention concerne également un procédé permettant d'analyser de tels effets enzymatiques qui modifient l'état topologique de l'ADN. De telles molécules d'acide nucléique et de tels procédés peuvent être utilisés en recherche pharmaceutique dans la recherche automatique de cytostatiques ou d'antibiotiques.
PCT/DE1998/003466 1997-11-21 1998-11-19 Molecule d'acide nucleique liee a la matrice et topologiquement fixee Ceased WO1999027131A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP98963380A EP0975808A2 (fr) 1997-11-21 1998-11-19 Molecule d'acide nucleique liee a la matrice et topologiquement fixee

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19753182.2 1997-11-21
DE19753182A DE19753182A1 (de) 1997-11-21 1997-11-21 Topologisch fixierte matrixgebundene Nukleinsäure

Publications (2)

Publication Number Publication Date
WO1999027131A2 WO1999027131A2 (fr) 1999-06-03
WO1999027131A3 true WO1999027131A3 (fr) 1999-10-07

Family

ID=7850347

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1998/003466 Ceased WO1999027131A2 (fr) 1997-11-21 1998-11-19 Molecule d'acide nucleique liee a la matrice et topologiquement fixee

Country Status (3)

Country Link
EP (1) EP0975808A2 (fr)
DE (1) DE19753182A1 (fr)
WO (1) WO1999027131A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001270098A1 (en) * 2000-06-22 2002-01-02 San Diego State University Foundation Recombination modulators and methods for their production and use
GB0424953D0 (en) 2004-11-11 2004-12-15 Plant Bioscience Ltd Assay
EP2462924A3 (fr) 2006-10-13 2013-01-23 Marina Biotech, Inc. Améliorations et ou associées aux liposomes amphotériques
EP2125031B1 (fr) 2006-12-19 2017-11-01 Marina Biotech, Inc. Lipides et ensembles lipidiques comprenant des éléments d'amélioration de la transfection
DE102007029471A1 (de) 2007-06-20 2008-12-24 Novosom Ag Neue fakultativ kationische Sterole
CN104744310A (zh) 2009-07-09 2015-07-01 玛瑞纳生物技术有限公司 包含亚氨基脂质的两性脂质体

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE59108783D1 (de) * 1990-04-12 1997-08-21 Hans Dr Sigrist Verfahren zur lichtinduzierten immobilisierung von biomolekülen an chemisch "inerten" oberflächen
DE19648625A1 (de) * 1996-11-13 1998-05-14 Soft Gene Gmbh Mikroprojektil für das Einbringen von Substanzen in Zellen durch ballistischen Transfer
EP1591541B1 (fr) * 1997-04-01 2012-02-15 Illumina Cambridge Limited Methode de séquençage d'acide nucléique

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ARNDT-JOVIN D. ET AL.,: "Z-DNA binding and inhibition by GTP of Drosophila topoisomeraes II", BIOCHEMISTRY, vol. 32, - 1993, pages 4862 - 4872, XP002111474 *
GLIKIN G.C. ET AL.,: "Interactions of drosophila DNA topoisomerase II with left-handed Z-DNA in supercoiled minicircles", NUCLEIC ACIDS RESEARCH, vol. 19, no. 25, - 1991, pages 7139 - 7144, XP002111475 *
THOMAS T.J.& THOMAS T.: "Direct evidence for the presence of left-handed conformation in a supramolecular assembly of polynucleotides", NUCLEIC ACIDS RESEARCH, vol. 17, - 1989, pages 3795 - 3810, XP002111473 *

Also Published As

Publication number Publication date
DE19753182A1 (de) 1999-07-29
EP0975808A2 (fr) 2000-02-02
WO1999027131A2 (fr) 1999-06-03

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