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WO1999025864A3 - HIGH MOLECULAR WEIGHT η-POLY(GLUTAMIC ACID) - Google Patents

HIGH MOLECULAR WEIGHT η-POLY(GLUTAMIC ACID) Download PDF

Info

Publication number
WO1999025864A3
WO1999025864A3 PCT/US1998/024580 US9824580W WO9925864A3 WO 1999025864 A3 WO1999025864 A3 WO 1999025864A3 US 9824580 W US9824580 W US 9824580W WO 9925864 A3 WO9925864 A3 WO 9925864A3
Authority
WO
WIPO (PCT)
Prior art keywords
pga
molecular weight
high molecular
poly
glutamic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/024580
Other languages
French (fr)
Other versions
WO1999025864A2 (en
Inventor
Alex Diiorio
Louis Stock
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Worcester Polytechnic Institute
Original Assignee
Worcester Polytechnic Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Worcester Polytechnic Institute filed Critical Worcester Polytechnic Institute
Priority to CA002310631A priority Critical patent/CA2310631A1/en
Priority to EP98960252A priority patent/EP1032699A1/en
Publication of WO1999025864A2 publication Critical patent/WO1999025864A2/en
Publication of WO1999025864A3 publication Critical patent/WO1999025864A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polyamides (AREA)

Abstract

High molecular weight η-PGA is provided. The η-PGA is separated from cells of the η-PGA producing cell culture after first adjusting the pH of the cell-culture to a pH of less than about 3. Also, the viscosity of a η-PGA solution is increased by removing metal ions with a chelator. In addition, η-PGA is fractionated directly from a cell culture, such that the fractionated η-PGA has low polydispersity. Purified η-PGA is produced having a molecular weight of at least 2.5 x 106.
PCT/US1998/024580 1997-11-18 1998-11-18 HIGH MOLECULAR WEIGHT η-POLY(GLUTAMIC ACID) Ceased WO1999025864A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002310631A CA2310631A1 (en) 1997-11-18 1998-11-18 High molecular weight .gamma.-poly(glutamic acid)
EP98960252A EP1032699A1 (en) 1997-11-18 1998-11-18 High molecular weight gamma-poly(glutamic acid)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6694297P 1997-11-18 1997-11-18
US60/066,942 1997-11-18

Publications (2)

Publication Number Publication Date
WO1999025864A2 WO1999025864A2 (en) 1999-05-27
WO1999025864A3 true WO1999025864A3 (en) 1999-12-23

Family

ID=22072718

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/024580 Ceased WO1999025864A2 (en) 1997-11-18 1998-11-18 HIGH MOLECULAR WEIGHT η-POLY(GLUTAMIC ACID)

Country Status (3)

Country Link
EP (1) EP1032699A1 (en)
CA (1) CA2310631A1 (en)
WO (1) WO1999025864A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040063612A1 (en) * 2002-09-26 2004-04-01 Manssur Yalpani Neuroprotective agents
DE102004030938A1 (en) 2004-06-26 2006-01-12 Henkel Kgaa New, polyamino acid-forming or degrading gene products of Bacillus licheniformis and improved biotechnological production processes based thereon
KR101697552B1 (en) 2010-04-09 2017-02-01 내셔날 헬스 리서치 인스티튜트 Gamma-polyglutamic acid-based ocular irrigating solutions
CN103665371B (en) * 2013-11-14 2016-03-02 天津北洋百川生物技术有限公司 A kind of method utilizing polyglutamic acid in ultrafiltration nanofiltration refined biological fermented liquid
CN109609408B (en) * 2018-12-27 2022-04-15 黄河三角洲京博化工研究院有限公司 Gamma-polyglutamic acid high-yield strain and method for preparing gamma-polyglutamic acid by using strain for liquid fermentation
CN112480394A (en) * 2020-12-01 2021-03-12 广西大学 Method for separating and purifying ultra-high molecular weight poly-gamma-glutamic acid from high-viscosity fermentation liquor

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
A.M. CROMWICK ET AL: "Effects of manganese (II) on Bacillus licheniformis ATCC 9945A physiology and GAmma-poly(glutamic acid) formation", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 17, no. 5, October 1995 (1995-10-01), pages 259 - 267, XP002103918 *
A.M. CROMWICK ET AL: "Effects of pH and aeration on gamma-poly(glutamic acid) formation by Bacillus licheniformis in controlled fermentor cultures", BIOTECHNOLOGY AND BIOENGINEERING. INCLUDING: SYMPOSIUM BIOTECHNOLOGY IN ENERGY PRODUCTION AND CONSERVATION., vol. 50, 1996, NEW YORK US, pages 222 - 227, XP002103912 *
ATSUO GOTO ET AL: "Biosynthesis and hydrolysis of poly(gamma-glutamic acid) from Bacillus subtilis IFO3335", BIOSCIENCE BIOTECHNOLOGY BIOCHEMISTRY, vol. 56, no. 7, 1992, pages 1031 - 1035, XP002103915 *
CROMWICK A.M. ET AL: "Investigation by NMR of metabolic routes to bacterial gamma-poly(glutaic acid) using 13C-labelled citrate and glutamate as media carbon sources", CANADIAN JOURNAL OF MICROBIOLOGY, vol. 41, no. 10, 1995, pages 902 - 909, XP002103919 *
G.A. BIRRER ET AL: "Gamma-poly(lutamic acid ) formation by Bacillus licheniformis 9945a: physiological and and biochemical studies.", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 16, no. 5, 1994, pages 265 - 275, XP002103917 *
MASAO KUNIOKA ET AL: "Biosynthesis of Poly(Gamma-glutamic acid from L-glutamic acid) , citric acid and ammonium sulfate in Bacillus subtilis IFO3335", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 40, 1994, pages 867 - 872, XP002103916 *
R. STOCK: "Rheological characterization of (poly)-gamma glutamic acid fermentations", ABSTRACTS OF PAPERS AMERICAN CHEMICAL SOCIETY, vol. 211, no. 1-2, May 1996 (1996-05-01), pages biot28, XP002103914 *
S.A. GIANNOS ET AL: "The biosynthesis of unusual polyamides containing glutamic acid", PROCEEDINGS OF THE ACS DIVISION OF POLYMERIC MATERIALS SCIENCE AND ENGINEERING, vol. 62, 1990, pages 236 - 240, XP002103913 *

Also Published As

Publication number Publication date
WO1999025864A2 (en) 1999-05-27
CA2310631A1 (en) 1999-05-27
EP1032699A1 (en) 2000-09-06

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