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WO1999015543A3 - Verfahren zum sequenzieren eines einzelnen dna-moleküls - Google Patents

Verfahren zum sequenzieren eines einzelnen dna-moleküls Download PDF

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Publication number
WO1999015543A3
WO1999015543A3 PCT/EP1998/005061 EP9805061W WO9915543A3 WO 1999015543 A3 WO1999015543 A3 WO 1999015543A3 EP 9805061 W EP9805061 W EP 9805061W WO 9915543 A3 WO9915543 A3 WO 9915543A3
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WIPO (PCT)
Prior art keywords
fibre
light
point
dna molecule
dyed
Prior art date
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Ceased
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PCT/EP1998/005061
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English (en)
French (fr)
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WO1999015543A2 (de
Inventor
Juergen Wolfrum
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Priority to US09/509,579 priority Critical patent/US6225068B1/en
Priority to EP98946308A priority patent/EP1017708A2/de
Publication of WO1999015543A2 publication Critical patent/WO1999015543A2/de
Publication of WO1999015543A3 publication Critical patent/WO1999015543A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Eine ausgezogene Faserspitze (1) wird mit Photobiotin belegt und mit UV-Licht bestrahlt. Dadurch geht das Photobiotin mit der Faserspitze in Bindung. Die Faserspitze wird anschließend mit Streptavidin in Kontakt gebracht und in eine Lösung getaucht, die gefärbte Kopien des zu sequenzierenden DNA-Moleküls enthält. Am 5'-Ende der gefärbten DNA-Moleküle (4A) ist ebenfalls Biotin gebunden. Das an die einzelnen DNA-Moleküle gekoppelte Biotin bindet an das Streptavidin an der Faserspitze. Zum Nachweis dieser Bindung wird Licht in die Faser eingekoppelt. Das evaneszente Feld dieses Lichts regt ein gefärbtes DNA-Molekül zur Fluoreszenz an, sobald es an die Faserspitze bindet. Wird eine Bindung festgestellt, wird die Faser sofort aus der Lösung entfernt. Die Faserspitze wird sodann in eine Mikrokapillare (5) eingeführt. Die Mikrokapillare wird mit einer Pufferlösung gefüllt, die Exonukleasen enthält, die das DNA-Molekül Base für Base abbauen. Der Ausgang (6) der Kapillare befindet sich im Beobachtungsbereich einer Einzelmolekül-Detekionsapparatur. Um das Signal-Rauschen-Verhältnis für die Einzel-molekül-Detektion zu verbessern, ist die Wandstärke der Mikrokapillare im Bereich der Austrittsöffnung deutlich kleiner als 1/4 der Wellenlänge des Anregungslichts.
PCT/EP1998/005061 1997-09-25 1998-08-10 Verfahren zum sequenzieren eines einzelnen dna-moleküls Ceased WO1999015543A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US09/509,579 US6225068B1 (en) 1997-09-25 1998-08-10 Process for sequencing an individual DNA molecule
EP98946308A EP1017708A2 (de) 1997-09-25 1998-08-10 Verfahren zum sequenzieren eines einzelnen dna-moleküls

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19742227.6 1997-09-25
DE19742227A DE19742227A1 (de) 1997-09-25 1997-09-25 Verfahren zum Sequenzieren eines einzelnen DNA-Moleküls

Publications (2)

Publication Number Publication Date
WO1999015543A2 WO1999015543A2 (de) 1999-04-01
WO1999015543A3 true WO1999015543A3 (de) 1999-05-20

Family

ID=7843525

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/005061 Ceased WO1999015543A2 (de) 1997-09-25 1998-08-10 Verfahren zum sequenzieren eines einzelnen dna-moleküls

Country Status (4)

Country Link
US (1) US6225068B1 (de)
EP (1) EP1017708A2 (de)
DE (1) DE19742227A1 (de)
WO (1) WO1999015543A2 (de)

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HUP0003296A3 (en) 1997-07-22 2001-09-28 Qiagen Genomics Inc Bothell Apparatus and methods for arraying solution onto a solid support
WO1999034214A1 (en) * 1997-12-31 1999-07-08 Qiagen Genomics, Inc Solid-phase tips and uses relating thereto
US6767731B2 (en) * 2001-08-27 2004-07-27 Intel Corporation Electron induced fluorescent method for nucleic acid sequencing
US6972173B2 (en) * 2002-03-14 2005-12-06 Intel Corporation Methods to increase nucleotide signals by raman scattering
US6982165B2 (en) 2001-09-24 2006-01-03 Intel Corporation Nucleic acid sequencing by raman monitoring of molecular deconstruction
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US7238477B2 (en) * 2001-09-24 2007-07-03 Intel Corporation Methods to increase nucleotide signals by Raman scattering
DE10162536A1 (de) * 2001-12-19 2003-07-17 Gnothis Holding Sa Ecublens Evaneszenz-basierendes Multiplex-Sequenzierungsverfahren
US20040110208A1 (en) * 2002-03-26 2004-06-10 Selena Chan Methods and device for DNA sequencing using surface enhanced Raman scattering (SERS)
US7476501B2 (en) 2002-03-26 2009-01-13 Intel Corporation Methods and device for DNA sequencing using surface enhanced raman scattering (SERS)
US7361821B2 (en) * 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
JP4520857B2 (ja) * 2002-09-20 2010-08-11 インテル・コーポレーション 走査型プローブ顕微鏡検査（ｓｐｍ）読取用の特定の情報をエンコードするナノバーコードの制御された整列
US7606403B2 (en) 2002-10-17 2009-10-20 Intel Corporation Model-based fusion of scanning probe microscopic images for detection and identification of molecular structures
US20050147976A1 (en) * 2003-12-29 2005-07-07 Xing Su Methods for determining nucleotide sequence information
US20050147979A1 (en) * 2003-12-30 2005-07-07 Intel Corporation Nucleic acid sequencing by Raman monitoring of uptake of nucleotides during molecular replication
US20050147980A1 (en) * 2003-12-30 2005-07-07 Intel Corporation Nucleic acid sequencing by Raman monitoring of uptake of nucleotides during molecular replication
EP1607737A1 (de) * 2004-06-17 2005-12-21 Patrick Hunziker Gerät und Methode zur Detektion und Messung von Nukleinsäuresträngen in Lösung
US20060057585A1 (en) * 2004-09-10 2006-03-16 Mcallister William H Nanostepper/sensor systems and methods of use thereof
US7736818B2 (en) 2004-12-27 2010-06-15 Inphase Technologies, Inc. Holographic recording medium and method of making it
DE102011008788B3 (de) * 2011-01-14 2012-05-03 Institut Für Photonische Technologien E.V. Anordnung zur Durchführung einer Einzelprobenanalyse und -manipulation für die Raman-Mikrospektroskopie
AU2016306451B2 (en) * 2015-08-11 2022-03-31 Stem Arts Projects, Llc Portable nucleic acid extraction apparatus and method of using the same

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DE3807975A1 (de) * 1988-03-10 1989-09-28 Greulich Karl Otto Verfahren zur optischen charakterisierung von nukleinsaeuren und oligonukleotiden
US5401469A (en) * 1989-04-19 1995-03-28 Ibiden Co., Ltd. Plastic optical biomaterials assay device
EP0563998A1 (de) * 1992-04-02 1993-10-06 Markus Sauer Verfahren zur Erfassung von Biomolekülen, toxischen Substanzen, Polymeren und pharmazeutischen Wirkstoffen mittels zeitaufgelöster Laserspektroskopie
DE4307042A1 (de) * 1993-03-05 1994-09-08 S & L Ges Fuer Wissenschaftlic Verfahren zum optischen qualitativen und quantitativen Nachweis von Molekülen, Biomolekülen und Mikroorganismen
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Also Published As

Publication number Publication date
US6225068B1 (en) 2001-05-01
WO1999015543A2 (de) 1999-04-01
DE19742227A1 (de) 1999-04-01
EP1017708A2 (de) 2000-07-12

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