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WO1999013051A1 - Dispositif permettant de purifier des matieres organiques par adsorption de ces matieres sur des billes et de retenir ces billes par des forces magnetiques, electriques ou gravitationnelles - Google Patents

Dispositif permettant de purifier des matieres organiques par adsorption de ces matieres sur des billes et de retenir ces billes par des forces magnetiques, electriques ou gravitationnelles Download PDF

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Publication number
WO1999013051A1
WO1999013051A1 PCT/NO1998/000271 NO9800271W WO9913051A1 WO 1999013051 A1 WO1999013051 A1 WO 1999013051A1 NO 9800271 W NO9800271 W NO 9800271W WO 9913051 A1 WO9913051 A1 WO 9913051A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
beads
liquid
attracting
particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/NO1998/000271
Other languages
English (en)
Inventor
Knut Rudi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genpoint AS
Original Assignee
Genpoint AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genpoint AS filed Critical Genpoint AS
Priority to AU91917/98A priority Critical patent/AU9191798A/en
Publication of WO1999013051A1 publication Critical patent/WO1999013051A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/28—Treatment of water, waste water, or sewage by sorption
    • C02F1/281—Treatment of water, waste water, or sewage by sorption using inorganic sorbents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption

Definitions

  • the present invention relates to a device for purification of organic material which is immobilised and retained on solid phases in a liquid solution.
  • the device is based on that the liquid flows through a container via separated 5 inlet and outlet.
  • the solid phase is retained either by physical coupling to the container or by magnetic, electric or gravitational forces.
  • Immobilisation of organic material on solid phases for purification is a known principle (international application no. PCT/GB91/00212).
  • the 0 purification strategies according to prior art are either based on that the liquid is sucked out with the same means with which it is supplied to the container, or the filtration of the liquid from the solid phase (products supplied by QIAGEN GmbH, Germany).
  • the problem with using the same means for supplying the contaminated liquid to the purification container and 5 the removal of the liquid after purification is that it is complicated and the risk for contamination is clearly increased.
  • To solve this problem by filtration complicates the whole process, since the filtrated particle has to be removed from the filter which tends to be clogged, or the organic material has to be eluted from the particles.
  • the object of the present invention is to provide a device for purifying organic material, such as for example DNA, RNA or proteins.
  • Fig. la - Id are cross sections of the device according to the invention showing the design and the different steps in the purification process.
  • the device according to the invention is illustrated in figure 1. It comprises a container 1 with an outlet opening 3 in the bottom of the container 1 connected to a tube with a discharge leg ascending on the outside of the container up to below the rim of the container, a U-bend 4 and a suction leg 5 ending below the bottom of the container.
  • the container is mounted on a vertically moveable platform 6 guided by two guide bars 7 mounted on a basis which in addition contains an attracting means 8; mounted such that when the container is in its lowest position the attracting means is closely adjacent to the container.
  • the platform 6 has two throughgoing holes for the section leg 5 of the outlet tube and the attracting means 8.
  • the basis has one throughgoing hole for the suction leg of the outlet tube 5, corresponding to the same hole in the platform 6.
  • the attracting means is a magnet or an electromagnetic device.
  • the electromagnetic device is then activated by suitable activating means, such as a circuit switch for activation of electrical energy.
  • suitable activating means such as a circuit switch for activation of electrical energy.
  • the organic material to be purified can be in solution or in solid state and is added to the container together with the solid phase comprising particles or beads or paramagnetic beads, or the beads are added to the container before the organic material to be purified, or after the organic material is added to the container.
  • Suitable liquid is then added to the container. To rinse out all material except the material of interest which is adsorbed to the solid phase, the level of the rinsing liquid is rised to reach the level corresponding to the U-bend 4 of the outlet tube, and the liquid will be sucked out of the container by gravitational forces until the container is empty.
  • the flow of liquid through the container 1 may also be powered by pumps.
  • the complexity of the device can be increased by mounting several containers together which can be moved vertically in relation to each other.
  • the magnetic means 8 will attract the solid phase when the container is moved to the lowest position (fig. lb). If the liquid is added to the container in this position it will when reaching the level corresponding to the U-bend flow out of the container while the particles will be kept in position by the magnetic forces. When the container is in the upper position the solid phase will not be affected by the magnetic forces, and by moving the container slightly up and down the mixture of particles and liquid will be facilitated.
  • This device can for example be used for purification of bacteria and then the same solid phase can be used for purifying the DNA from this bacteria.
  • the bacteria are mixed with a solution with paramagnetic particles and the bacteria will adhere to the solid phase.
  • the container will then be lowered (fig. lb) such that the solid phase with the bacteria adsorbed will be pulled towards the magnets.
  • Liquid will continuously be added to the container such that the critical liquid level for emptying the container is maintained.
  • the bacteria have obtained the desired purity the liquid is emptied out the container (fig lc,d) and the container is lifted to the upper position.
  • a new liquid for rinsing of bacterial debris from the DNA and binding the DNA to the solid phase is added, and mixing of the solution by moving the container up and down in the upper position is performed.
  • the container is then moved to the lowest position and the liquid level is kept on the critical level for emptying the container. This treatment is repeated until the DNA has reached the desired purity.
  • DNA associated with the beads can be used directly in enzymatic reactions, such as the polymerase chain reaction (PCR).
  • the purified DNA can be transferred to a new container, or the original container (1) can be used for downstream applications such as thermocycling in PCR.
  • the device according to the invention may have several embodiments: 1. All known beads for immobilising organic molecules can be used.
  • Liquid flow can be regulated by a pump.
  • the solid phase with the organic material can be retained either by being physically connected to a container, or by gravitational forces or electric forces. 4.
  • the liquid volumes can be varied. 5.
  • the number of containers used in the process at the same time can vary.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Hydrology & Water Resources (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Cette invention a trait à un dispositif permettant de purifier des matières organiques immobilisées et retenues en phase solide. Ce dispositif se caractérise par le fait que le liquide s'écoule dans un contenant pourvu d'orifices distincts d'entrée et de sortie. La phase solide est retenue, soit par couplage physique au contenant, soit par des forces magnétiques, électriques ou gravitationnelles. L'invention concerne également un procédé de purification.
PCT/NO1998/000271 1997-09-08 1998-09-07 Dispositif permettant de purifier des matieres organiques par adsorption de ces matieres sur des billes et de retenir ces billes par des forces magnetiques, electriques ou gravitationnelles Ceased WO1999013051A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU91917/98A AU9191798A (en) 1997-09-08 1998-09-07 Device for purification of organic material by adsorbing material to beads and attracting beads with magnetical, electrical or gravitational means

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NO974113 1997-09-08
NO974113A NO305370B1 (no) 1997-09-08 1997-09-08 Apparat for rensing av organisk materiale og fremgangsmÕte for rensing av organisk materiale

Publications (1)

Publication Number Publication Date
WO1999013051A1 true WO1999013051A1 (fr) 1999-03-18

Family

ID=19901087

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NO1998/000271 Ceased WO1999013051A1 (fr) 1997-09-08 1998-09-07 Dispositif permettant de purifier des matieres organiques par adsorption de ces matieres sur des billes et de retenir ces billes par des forces magnetiques, electriques ou gravitationnelles

Country Status (3)

Country Link
AU (1) AU9191798A (fr)
NO (1) NO305370B1 (fr)
WO (1) WO1999013051A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000075623A1 (fr) * 1999-06-08 2000-12-14 Dna Research Innovations Limited Dispositif de traitement d'echantillons

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983002405A1 (fr) * 1982-01-14 1983-07-21 Reed, Thomas, A. Procede et appareil pour separer les substances organiques d'une suspension ou solution
US5439586A (en) * 1993-09-15 1995-08-08 The Terry Fox Laboratory Of The British Columbia Cancer Agnecy Magnetic filter with ordered wire array
EP0687505A1 (fr) * 1994-06-16 1995-12-20 Roche Diagnostics GmbH Méthode de séparation magnétique pour des composants d'un liquide
US5622831A (en) * 1990-09-26 1997-04-22 Immunivest Corporation Methods and devices for manipulation of magnetically collected material

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983002405A1 (fr) * 1982-01-14 1983-07-21 Reed, Thomas, A. Procede et appareil pour separer les substances organiques d'une suspension ou solution
US5622831A (en) * 1990-09-26 1997-04-22 Immunivest Corporation Methods and devices for manipulation of magnetically collected material
US5439586A (en) * 1993-09-15 1995-08-08 The Terry Fox Laboratory Of The British Columbia Cancer Agnecy Magnetic filter with ordered wire array
EP0687505A1 (fr) * 1994-06-16 1995-12-20 Roche Diagnostics GmbH Méthode de séparation magnétique pour des composants d'un liquide

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000075623A1 (fr) * 1999-06-08 2000-12-14 Dna Research Innovations Limited Dispositif de traitement d'echantillons

Also Published As

Publication number Publication date
NO974113L (no) 1999-03-09
AU9191798A (en) 1999-03-29
NO974113D0 (no) 1997-09-08
NO305370B1 (no) 1999-05-18

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