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WO1999007876A3 - Methode de dosage des proteines cellulaires - Google Patents

Methode de dosage des proteines cellulaires Download PDF

Info

Publication number
WO1999007876A3
WO1999007876A3 PCT/EP1998/005041 EP9805041W WO9907876A3 WO 1999007876 A3 WO1999007876 A3 WO 1999007876A3 EP 9805041 W EP9805041 W EP 9805041W WO 9907876 A3 WO9907876 A3 WO 9907876A3
Authority
WO
WIPO (PCT)
Prior art keywords
cellular protein
assaying cellular
assaying
cells
fluorescent dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1998/005041
Other languages
German (de)
English (en)
Other versions
WO1999007876A2 (fr
Inventor
Stefan Postius
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda GmbH
Original Assignee
Byk Gulden Lomberg Chemische Fabrik GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Byk Gulden Lomberg Chemische Fabrik GmbH filed Critical Byk Gulden Lomberg Chemische Fabrik GmbH
Priority to AU93407/98A priority Critical patent/AU9340798A/en
Publication of WO1999007876A2 publication Critical patent/WO1999007876A2/fr
Publication of WO1999007876A3 publication Critical patent/WO1999007876A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/205—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Campylobacter (G)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode qui permet de déterminer des quantités de protéines cellulaires par fluorimétrie en utilisant un colorant fluorescent, sans préparation ni lyse préalable des cellules.
PCT/EP1998/005041 1997-08-12 1998-08-08 Methode de dosage des proteines cellulaires Ceased WO1999007876A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU93407/98A AU9340798A (en) 1997-08-12 1998-08-08 Method for assaying cellular protein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19734743 1997-08-12
DE19734743.6 1997-08-12

Publications (2)

Publication Number Publication Date
WO1999007876A2 WO1999007876A2 (fr) 1999-02-18
WO1999007876A3 true WO1999007876A3 (fr) 1999-04-29

Family

ID=7838633

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/005041 Ceased WO1999007876A2 (fr) 1997-08-12 1998-08-08 Methode de dosage des proteines cellulaires

Country Status (2)

Country Link
AU (1) AU9340798A (fr)
WO (1) WO1999007876A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110713734B (zh) * 2018-07-13 2020-12-25 北京化工大学 一种纳米荧光染料的制备方法
CN112666356A (zh) * 2020-12-31 2021-04-16 国家纳米科学中心 一种检测痕量蛋白质的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4433384A1 (de) * 1994-09-09 1996-03-14 Ufz Leipzighalle Gmbh Ökotoxikologische Stoffwechseluntersuchung mit stabilen Isotopen
WO1996036882A1 (fr) * 1995-05-19 1996-11-21 Molecular Probes, Inc. Coloration de proteines par colorant a base de merocyanine
DE19631020A1 (de) * 1996-08-01 1998-02-05 Odenthal Maria Dr Verfahren zur quantitativen Bestimmung von Protein

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4433384A1 (de) * 1994-09-09 1996-03-14 Ufz Leipzighalle Gmbh Ökotoxikologische Stoffwechseluntersuchung mit stabilen Isotopen
WO1996036882A1 (fr) * 1995-05-19 1996-11-21 Molecular Probes, Inc. Coloration de proteines par colorant a base de merocyanine
DE19631020A1 (de) * 1996-08-01 1998-02-05 Odenthal Maria Dr Verfahren zur quantitativen Bestimmung von Protein

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MOLECULAR PROBES INC.: "NanoOrange (TM) Protein Quantitation Kit (N-6666)", RETRIEVED FROM INTERNET: <URL: HTTP://WWW.PROBES.COM/MEDIA/PIS/MP06666.PDF>, 17 February 1999 (1999-02-17), XP002094750 *

Also Published As

Publication number Publication date
WO1999007876A2 (fr) 1999-02-18
AU9340798A (en) 1999-03-01

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