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WO1999005266A3 - Trans-species nuclear transfer - Google Patents

Trans-species nuclear transfer Download PDF

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Publication number
WO1999005266A3
WO1999005266A3 PCT/US1998/015387 US9815387W WO9905266A3 WO 1999005266 A3 WO1999005266 A3 WO 1999005266A3 US 9815387 W US9815387 W US 9815387W WO 9905266 A3 WO9905266 A3 WO 9905266A3
Authority
WO
WIPO (PCT)
Prior art keywords
nuclear transfer
trans
donor cells
species nuclear
oocytes
Prior art date
Application number
PCT/US1998/015387
Other languages
French (fr)
Other versions
WO1999005266A2 (en
WO1999005266B1 (en
Inventor
Neal L First
Tanja Dominko
Maissam Mitalipoua
Original Assignee
Wisconsin Alumni Res Found
Neal L First
Tanja Dominko
Maissam Mitalipoua
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found, Neal L First, Tanja Dominko, Maissam Mitalipoua filed Critical Wisconsin Alumni Res Found
Priority to AU85875/98A priority Critical patent/AU8587598A/en
Publication of WO1999005266A2 publication Critical patent/WO1999005266A2/en
Publication of WO1999005266A3 publication Critical patent/WO1999005266A3/en
Publication of WO1999005266B1 publication Critical patent/WO1999005266B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method of producing cloned nuclear transfer embryos from differentiated donor cells is described. The method includes culturing differentiated donor cells in low serum medium and using bovine oocytes as recipient oocytes for donor cells from different species. This method may be used to produce genetically identical animals and transgenic animals.
PCT/US1998/015387 1997-07-26 1998-07-24 Trans-species nuclear transfer WO1999005266A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU85875/98A AU8587598A (en) 1997-07-26 1998-07-24 Trans-species nuclear transfer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5310397P 1997-07-26 1997-07-26
US60/053,103 1997-07-26

Publications (3)

Publication Number Publication Date
WO1999005266A2 WO1999005266A2 (en) 1999-02-04
WO1999005266A3 true WO1999005266A3 (en) 1999-04-15
WO1999005266B1 WO1999005266B1 (en) 1999-09-23

Family

ID=21981947

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/015387 WO1999005266A2 (en) 1997-07-26 1998-07-24 Trans-species nuclear transfer

Country Status (2)

Country Link
AU (1) AU8587598A (en)
WO (1) WO1999005266A2 (en)

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IL138201A0 (en) * 1998-03-02 2001-10-31 Univ Massachusetts Embryonic or stem-like cell lines produced by cross-species nuclear transplantation
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US7621606B2 (en) 2001-08-27 2009-11-24 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
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WO1998007841A1 (en) * 1996-08-19 1998-02-26 University Of Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation
WO1998030683A2 (en) * 1997-01-10 1998-07-16 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts Nuclear transfer with differentiated fetal and adult donor cells

Patent Citations (4)

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US5496720A (en) * 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
WO1997007669A1 (en) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer
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Publication number Publication date
WO1999005266A2 (en) 1999-02-04
WO1999005266B1 (en) 1999-09-23
AU8587598A (en) 1999-02-16

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