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WO1999005265A3 - Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance - Google Patents

Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance Download PDF

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Publication number
WO1999005265A3
WO1999005265A3 PCT/US1998/015289 US9815289W WO9905265A3 WO 1999005265 A3 WO1999005265 A3 WO 1999005265A3 US 9815289 W US9815289 W US 9815289W WO 9905265 A3 WO9905265 A3 WO 9905265A3
Authority
WO
WIPO (PCT)
Prior art keywords
herbicide resistance
plants
production
plastid transformation
transgenic plants
Prior art date
Application number
PCT/US1998/015289
Other languages
French (fr)
Other versions
WO1999005265A2 (en
Inventor
Alan D Blowers
John Sanford
Ana Maria Bailey
Original Assignee
Sanford Scient Inc
Invest Y De Estudios Avanzados
Alan D Blowers
John Sanford
Ana Maria Bailey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanford Scient Inc, Invest Y De Estudios Avanzados, Alan D Blowers, John Sanford, Ana Maria Bailey filed Critical Sanford Scient Inc
Priority to AU85837/98A priority Critical patent/AU8583798A/en
Publication of WO1999005265A2 publication Critical patent/WO1999005265A2/en
Publication of WO1999005265A3 publication Critical patent/WO1999005265A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides a method of producing a herbicide-resistant plant, which method entails delivering one or more herbicide resistance-conferring selectable marker genes into and expressing the same within the plastid of the plant, both in photosynthetic as well as non-photosynthetic cells. Nucleic acids for transformation and multicellular plants whose plastids have been transformed are also provided.
PCT/US1998/015289 1997-07-23 1998-07-23 Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance WO1999005265A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU85837/98A AU8583798A (en) 1997-07-23 1998-07-23 Improved plastid transformation of higher plants and production of trans genic plants with herbicide resistance

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US89906197A 1997-07-23 1997-07-23
US08/899,061 1997-07-23

Publications (2)

Publication Number Publication Date
WO1999005265A2 WO1999005265A2 (en) 1999-02-04
WO1999005265A3 true WO1999005265A3 (en) 1999-05-20

Family

ID=25410433

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/015289 WO1999005265A2 (en) 1997-07-23 1998-07-23 Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance

Country Status (4)

Country Link
AR (1) AR015136A1 (en)
AU (1) AU8583798A (en)
WO (1) WO1999005265A2 (en)
ZA (1) ZA986574B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6858777B2 (en) 2000-08-11 2005-02-22 Syngenta Participations Ag Methods for stable transformation of plants
US7645918B2 (en) 2002-08-08 2010-01-12 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Method of plastid transformation in asteraceae, vector for use therein and plants thus obtained
US8946460B2 (en) 2012-06-15 2015-02-03 Commonwealth Scientific And Industrial Research Organisation Process for producing polyunsaturated fatty acids in an esterified form

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
US6492578B1 (en) * 1998-07-10 2002-12-10 Calgene Llc Expression of herbicide tolerance genes in plant plastids
ATE354660T1 (en) * 1998-07-10 2007-03-15 Calgene Llc EXPRESSION OF HERBICIDE TOLERANCE GENES IN PLANT PLASTIDS
CA2339084C (en) 1998-08-04 2013-01-08 Cargill, Incorporated Plant fatty acid desaturase promoters
US6515206B1 (en) 1998-12-23 2003-02-04 Calgene Llc Plastid transformation of Brassica
AU6277600A (en) * 1999-07-27 2001-02-13 Syngenta Participations Ag Novel chimeric genes
CN100425701C (en) 1999-12-16 2008-10-15 孟山都技术有限公司 Novel Plant Expression Constructs
US20030041353A1 (en) * 2001-04-18 2003-02-27 Henry Daniell Mutiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
WO2001064024A1 (en) * 2000-02-29 2001-09-07 Auburn University Multiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
DE60133727T2 (en) 2000-03-22 2008-07-24 Icon Genetics, Inc. PROCESS FOR TRANSFORMATION OF VEGETABLE PLASTIDES AND FOR THE PRODUCTION OF TRANSPLASTOMEN PLANTS
JP2003530888A (en) * 2000-04-20 2003-10-21 ビーティージー・インターナショナル・リミテッド Transgenic plants
AU2001257300A1 (en) 2000-04-26 2001-11-07 Monsanto Technology Llc Method for the transformation of plant cell plastids
AU2012200277B2 (en) * 2002-08-08 2014-11-06 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Method of plastid transformation in Asteraceae, vector for use therein and plants thus obtained
AU2007272314B2 (en) 2006-07-12 2014-05-01 Commonwealth Scientific And Industrial Research Organisation Polynucleotides and methods for enhancing salinity tolerance in plants
DK2610334T3 (en) * 2006-12-07 2017-01-30 Dow Agrosciences Llc HAVE UNKNOWN SELECTABLE MARKET GENERATIONS
JP2010531640A (en) 2007-06-29 2010-09-30 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション Method for decomposing toxic compounds
MX2010001734A (en) 2007-08-13 2010-08-04 Commw Scient Ind Res Org PRIMED WITH LOW LEVELS OF HORDEINAS.
EP2283137A4 (en) 2008-04-25 2011-12-14 Commw Scient Ind Res Org RECOMBINANT CELLS AND METHODS FOR HYDROXYLATION OF FATTY ACIDS
AU2009287411B2 (en) 2008-08-25 2013-11-07 Commonwealth Scientific And Industrial Research Organisation Resistance genes
CN113957105B (en) 2008-11-18 2024-11-01 联邦科学技术研究组织 Enzymes and methods for producing omega-3 fatty acids
EP2585589B1 (en) 2010-06-28 2017-06-21 Commonwealth Scientific and Industrial Research Organisation Methods of producing lipids
EP2798063B1 (en) 2011-12-27 2022-03-30 Commonwealth Scientific and Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
CN104271743B (en) 2011-12-27 2019-11-05 联邦科学技术研究组织 Methods of producing lipids
EP2798065B1 (en) 2011-12-27 2019-06-05 Commonwealth Scientific and Industrial Research Organisation Simultaneous gene silencing and supressing gene silencing in the same cell
NZ631602A (en) 2013-06-13 2019-09-27 Grains Res & Dev Corp Barley with very low levels of hordeins
WO2015024066A1 (en) 2013-08-21 2015-02-26 Commonwealth Scientific And Industrial Research Organisation Rust resistance gene
CN111154724B (en) 2013-12-18 2024-02-06 联邦科学技术研究组织 Extracted plant lipids containing docosahexaenoic acid
CA2953008C (en) 2014-06-27 2024-03-19 Nuseed Pty Ltd Lipid comprising docosapentaenoic acid
PH12016502588B1 (en) 2014-07-07 2024-01-17 Commw Scient Ind Res Org Processes for producing industrial products from plant lipids
US11452272B2 (en) 2015-11-18 2022-09-27 Commonwealth Scientific And Industrial Research Organisation Rice grain with reduced ROS1A activity
US10941428B2 (en) 2018-09-12 2021-03-09 Universidad Politécnica de Madrid Reagents and methods for the expression of an active NifB protein and uses thereof
KR20210149063A (en) 2019-03-08 2021-12-08 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 Expression of nitrogenase polypeptides in plant cells

Citations (7)

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Publication number Priority date Publication date Assignee Title
EP0223247A2 (en) * 1985-11-22 1987-05-27 Ciba-Geigy Ag Direct gene transfer into plastids and mitochondria
EP0251654A2 (en) * 1986-06-23 1988-01-07 Biotechnica International, Inc. Transformation of chloroplasts
US5217902A (en) * 1989-05-26 1993-06-08 Dna Plant Technology Corporation Method of introducing spectinomycin resistance into plants
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
WO1997032977A1 (en) * 1996-03-06 1997-09-12 Rutgers University Plastid transformation in arabidopsis thaliana
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
WO1998002562A2 (en) * 1996-07-16 1998-01-22 Rhone-Poulenc Agrochimie Chimera gene with several herbicide resistant genes, plant cell and plant resistant to several herbicides

Patent Citations (7)

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EP0223247A2 (en) * 1985-11-22 1987-05-27 Ciba-Geigy Ag Direct gene transfer into plastids and mitochondria
EP0251654A2 (en) * 1986-06-23 1988-01-07 Biotechnica International, Inc. Transformation of chloroplasts
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5217902A (en) * 1989-05-26 1993-06-08 Dna Plant Technology Corporation Method of introducing spectinomycin resistance into plants
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
WO1997032977A1 (en) * 1996-03-06 1997-09-12 Rutgers University Plastid transformation in arabidopsis thaliana
WO1998002562A2 (en) * 1996-07-16 1998-01-22 Rhone-Poulenc Agrochimie Chimera gene with several herbicide resistant genes, plant cell and plant resistant to several herbicides

Non-Patent Citations (7)

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Title
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D'HALLUIN K ET AL: "TRANSFORMATION OF SUGARBEET (BETA VULGARIS L.) AND EVALUATION OF HERBICIDE RESISTANCE IN TRANSGENIC PLANTS", BIO/TECHNOLOGY, vol. 10, March 1992 (1992-03-01), pages 309 - 314, XP002056296 *
RATHORE K S ET AL: "USE OF BAR AS A SELECTABLE MARKER GENE AND FOR THE PRODUCTION OF HERBICIDE-RESISTANT RICE PLANTS FROM PROTOPLASTS", PLANT MOLECULAR BIOLOGY, vol. 21, no. 5, March 1993 (1993-03-01), pages 871 - 884, XP002064546 *
WOHLLEBEN W ET AL: "NUCLEOTIDE SEQUENCE OF THE PHOSPHINOTHRICIN N-ACETYLTRANSFERASE GENE FROM STREPTOMYCES VIRIDOCHROMOGENES TUE494 AND ITS EXPRESSION IN NICOTIANA TABACUM", GENE, vol. 70, 1988, pages 25 - 37, XP002071460 *
ZORA SVAB ET AL: "STABLE TRANSFORMATION OF PLASTIDS IN HIGHER PLANTS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 87, no. 21, 1 November 1990 (1990-11-01), pages 8526 - 8530, XP000164753 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6858777B2 (en) 2000-08-11 2005-02-22 Syngenta Participations Ag Methods for stable transformation of plants
US7645918B2 (en) 2002-08-08 2010-01-12 Rijk Zwaan Zaadteelt En Zaadhandel B.V. Method of plastid transformation in asteraceae, vector for use therein and plants thus obtained
US8946460B2 (en) 2012-06-15 2015-02-03 Commonwealth Scientific And Industrial Research Organisation Process for producing polyunsaturated fatty acids in an esterified form

Also Published As

Publication number Publication date
AR015136A1 (en) 2001-04-18
AU8583798A (en) 1999-02-16
ZA986574B (en) 2000-01-12
WO1999005265A2 (en) 1999-02-04

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