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WO1999066032A3 - Cells, genetically modified with optical marker - Google Patents

Cells, genetically modified with optical marker Download PDF

Info

Publication number
WO1999066032A3
WO1999066032A3 PCT/IB1999/001092 IB9901092W WO9966032A3 WO 1999066032 A3 WO1999066032 A3 WO 1999066032A3 IB 9901092 W IB9901092 W IB 9901092W WO 9966032 A3 WO9966032 A3 WO 9966032A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
fluorescence
genetically modified
optical marker
surveillance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB1999/001092
Other languages
French (fr)
Other versions
WO1999066032A2 (en
Inventor
Martin Jordan
Florian Maria Wurm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ecole Polytechnique Federale de Lausanne EPFL
Original Assignee
Ecole Polytechnique Federale de Lausanne EPFL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecole Polytechnique Federale de Lausanne EPFL filed Critical Ecole Polytechnique Federale de Lausanne EPFL
Priority to AU40543/99A priority Critical patent/AU4054399A/en
Priority to EP99923794A priority patent/EP1088058A2/en
Publication of WO1999066032A2 publication Critical patent/WO1999066032A2/en
Publication of WO1999066032A3 publication Critical patent/WO1999066032A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
    • G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Fluorescent protein expressing cells with very high fluorescence are described as well as a method to obtain such cells. The fluorescence of such cells can be as high as about 1000 times the auto-fluorescence of the respective host cell. Due to the high fluorescence, said cells are very suitable tools in the development, surveillance and/or optimization of production processes, for selection of culture media and process parameters and for the determination of cell growth and the detection, quantification and monitoring of biomass.
PCT/IB1999/001092 1998-06-16 1999-06-14 Cells, genetically modified with optical marker Ceased WO1999066032A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU40543/99A AU4054399A (en) 1998-06-16 1999-06-14 Cells, genetically modified with optical marker
EP99923794A EP1088058A2 (en) 1998-06-16 1999-06-14 Cells, genetically modified with optical marker

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/098,284 1998-06-16
US09/098,284 US6509174B2 (en) 1998-06-16 1998-06-16 Cells, genetically modified with optical marker

Publications (2)

Publication Number Publication Date
WO1999066032A2 WO1999066032A2 (en) 1999-12-23
WO1999066032A3 true WO1999066032A3 (en) 2000-04-20

Family

ID=22268622

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1999/001092 Ceased WO1999066032A2 (en) 1998-06-16 1999-06-14 Cells, genetically modified with optical marker

Country Status (4)

Country Link
US (1) US6509174B2 (en)
EP (1) EP1088058A2 (en)
AU (1) AU4054399A (en)
WO (1) WO1999066032A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7015310B2 (en) 2001-03-12 2006-03-21 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Oxidation reduction sensitive green fluorescent protein variants
US20100255999A1 (en) * 2007-02-01 2010-10-07 Dana-Farber Cancer Institute, Inc. Cell Co-Culture Systems and Uses Thereof
US20090088341A1 (en) * 2007-10-01 2009-04-02 The Government Of The Usa As Represented By The Secretary Of The Dept. Of Health & Human Services Multiplex assay method for mixed cell populations
US8679836B2 (en) * 2007-10-01 2014-03-25 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods of monitoring angiogenesis and metastasis in three dimensional co-cultures
CN113740531B (en) * 2021-09-01 2023-02-03 陕西脉元生物科技有限公司 Cell for reducing fluorescence nonspecific color development, detection product based on cell and application of cell in detecting autoantibody

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997026333A1 (en) * 1996-01-18 1997-07-24 University Of Florida Research Foundation, Incorporated Humanized green fluorescent protein genes and methods

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665589A (en) * 1988-12-14 1997-09-09 The United States Of America As Represented By The Department Of Health And Human Services Human liver epithelial cell lines
ATE263837T1 (en) 1991-05-31 2004-04-15 Genentech Inc EXPRESSION INCREASE THROUGH GENTARGETING INTO ENDOGENE RETROVIRUS-LIKE SEQUENCES
US5484720A (en) 1994-09-08 1996-01-16 Genentech, Inc. Methods for calcium phosphate transfection
US5968738A (en) * 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
US6027881A (en) 1996-05-08 2000-02-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997026333A1 (en) * 1996-01-18 1997-07-24 University Of Florida Research Foundation, Incorporated Humanized green fluorescent protein genes and methods

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DOEVENDANS, P.A. ET AL.: "The utility of fluorescent in vivo reporter genes in molecular cardiology", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 222, no. 2, 1996, pages 352 - 358, XP002129670 *
PRASHER, D.C.: "Using GFP to see the light", TRENDS IN GENETICS, vol. 11, no. 8, 1 August 1995 (1995-08-01), pages 320 - 323, XP002029559 *
ZHANG, G. ET AL.: "An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 227, no. 3, 1996, pages 707 - 711, XP002129669 *

Also Published As

Publication number Publication date
EP1088058A2 (en) 2001-04-04
US6509174B2 (en) 2003-01-21
AU4054399A (en) 2000-01-05
WO1999066032A2 (en) 1999-12-23
US20020058334A1 (en) 2002-05-16

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