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WO1999064562A3 - Expression control elements from genes encoding starch branching enzymes - Google Patents

Expression control elements from genes encoding starch branching enzymes Download PDF

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Publication number
WO1999064562A3
WO1999064562A3 PCT/US1999/013266 US9913266W WO9964562A3 WO 1999064562 A3 WO1999064562 A3 WO 1999064562A3 US 9913266 W US9913266 W US 9913266W WO 9964562 A3 WO9964562 A3 WO 9964562A3
Authority
WO
WIPO (PCT)
Prior art keywords
genes encoding
starch branching
branching enzymes
control elements
expression control
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/013266
Other languages
French (fr)
Other versions
WO1999064562A2 (en
Inventor
Mark J Guiltinan
Kyung-Nam Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pennsylvania State University
University of Pennsylvania Penn
Original Assignee
Pennsylvania State University
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pennsylvania State University, University of Pennsylvania Penn filed Critical Pennsylvania State University
Priority to AU44384/99A priority Critical patent/AU4438499A/en
Publication of WO1999064562A2 publication Critical patent/WO1999064562A2/en
Publication of WO1999064562A3 publication Critical patent/WO1999064562A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Nucleic acid molecules for directing and modulating gene expression in transformed cells are disclosed. The molecules comprise 5' and 3' regulatory sequences isolated from genomic clones having homology in their coding regions with maize genes encoding Type I and Type IIB starch branching enzymes. Chimeric genes comprising these expression controlling nucleic acid molecules, and cells transformed with such chimeric genes, are also disclosed.
PCT/US1999/013266 1998-06-12 1999-06-11 Expression control elements from genes encoding starch branching enzymes Ceased WO1999064562A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU44384/99A AU4438499A (en) 1998-06-12 1999-06-11 Expression control elements from genes encoding starch branching enzymes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US8905098P 1998-06-12 1998-06-12
US8904998P 1998-06-12 1998-06-12
US60/089,049 1998-06-12
US60/089,050 1998-06-12

Publications (2)

Publication Number Publication Date
WO1999064562A2 WO1999064562A2 (en) 1999-12-16
WO1999064562A3 true WO1999064562A3 (en) 2000-05-18

Family

ID=26780093

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/013266 Ceased WO1999064562A2 (en) 1998-06-12 1999-06-11 Expression control elements from genes encoding starch branching enzymes

Country Status (2)

Country Link
AU (1) AU4438499A (en)
WO (1) WO1999064562A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ332928A (en) * 1996-06-25 2000-01-28 Bayer Ag Ozone-induced gene expression in plants and DNA seOzone-induced gene expression in plants and DNA sequences derived from grapevine quences derived from grapevine
CN1295621A (en) * 1998-04-01 2001-05-16 杰尼克莫根有限公司 Pathogen-inducible promoter
CN102965394A (en) * 2012-09-10 2013-03-13 四川农业大学 Method for transient expression of exogenous gene by maize endosperm

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5290924A (en) * 1987-02-06 1994-03-01 Last David I Recombinant promoter for gene expression in monocotyledonous plants
US5608149A (en) * 1990-06-18 1997-03-04 Monsanto Company Enhanced starch biosynthesis in tomatoes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5290924A (en) * 1987-02-06 1994-03-01 Last David I Recombinant promoter for gene expression in monocotyledonous plants
US5608149A (en) * 1990-06-18 1997-03-04 Monsanto Company Enhanced starch biosynthesis in tomatoes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BABA T. ET AL: "Sequence conservation of the catalytic regions of amylolytic enzymes in maize branching enzyme-I", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 181, no. 1, 27 November 1991 (1991-11-27), pages 87 - 94, XP002923867 *
FISHER D.K. ET AL: "A cDNA encoding starch branching enzyme I from maize endosperm", PLANT PHYSIOLOGY, vol. 108, no. 3, July 1995 (1995-07-01), USA, pages 1313 - 1314, XP002031586 *
FISHER D.K. ET AL: "Starch branching enzyme II from maize endosperm", PLANT PHYSIOLOGY, vol. 102, no. 3, July 1993 (1993-07-01), USA, pages 1045 - 1046, XP002031585 *
GIROUX M.J. ET AL: "Coordinated transcriptional regulation of storage product genes in the maize endosperm", PLANT PHYSIOLOGY, vol. 106, 1994, pages 713 - 722, XP002923869 *
KAWASAKY T. ET AL: "Molecular analysis of the gene encoding a rice starch branching enzyme", MOLECULAR GENERAL GENETICS, vol. 237, 1993, pages 10 - 16, XP002923868 *

Also Published As

Publication number Publication date
WO1999064562A2 (en) 1999-12-16
AU4438499A (en) 1999-12-30

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