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WO1999062316A3 - Identification of lactic acid bacteria genes involved in the biosynthesis of exopolysaccharides - Google Patents

Identification of lactic acid bacteria genes involved in the biosynthesis of exopolysaccharides Download PDF

Info

Publication number
WO1999062316A3
WO1999062316A3 PCT/EP1999/002841 EP9902841W WO9962316A3 WO 1999062316 A3 WO1999062316 A3 WO 1999062316A3 EP 9902841 W EP9902841 W EP 9902841W WO 9962316 A3 WO9962316 A3 WO 9962316A3
Authority
WO
WIPO (PCT)
Prior art keywords
exopolysaccharides
biosynthesis
identification
lactic acid
acid bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1999/002841
Other languages
French (fr)
Other versions
WO1999062316A2 (en
Inventor
Francesca Stingele
Jacques Edouard Germond
Gilbert Lamothe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Societe des Produits Nestle SA
Nestle SA
Original Assignee
Societe des Produits Nestle SA
Nestle SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP98201310A external-priority patent/EP0957168A1/en
Priority claimed from EP98201311A external-priority patent/EP0957169A1/en
Priority claimed from EP98201312A external-priority patent/EP0957170A1/en
Application filed by Societe des Produits Nestle SA, Nestle SA filed Critical Societe des Produits Nestle SA
Priority to AU59696/99A priority Critical patent/AU5969699A/en
Publication of WO1999062316A2 publication Critical patent/WO1999062316A2/en
Publication of WO1999062316A3 publication Critical patent/WO1999062316A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention concerns a recombined enzyme capable of being involved in exopolysaccharide biosynthesis by binding in the form of an isomer α or β between the carbon 1 bearing the aldehyde reduction function of an activated D-Galp and a lipophilic phosphate primer, in particular of the undecaprenol or protein type, particularly of the ACP type (Acyl Carrier Protein), so as to gradually ensure the formation of said exopolysaccharides including at each step the addition of a novel saccharide unit which is urged to be bound by its semi-acetal function to an alcoholic hydroxyl of another saccharide unit, which is at the end of a saccharide chain bound to said primer.
PCT/EP1999/002841 1998-04-22 1999-04-22 Identification of lactic acid bacteria genes involved in the biosynthesis of exopolysaccharides Ceased WO1999062316A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU59696/99A AU5969699A (en) 1998-04-22 1999-04-22 Lactic acid bacteria producing exopolysaccharides

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP98201310A EP0957168A1 (en) 1998-04-22 1998-04-22 Identification of Streptococcus thermophilus Sfi39 genes involved in the biosynthesis of exopolysaccharides
EP98201311A EP0957169A1 (en) 1998-04-22 1998-04-22 Identification of Lactobacillus helveticus LH59 genes involved in the biosynthesis of exopolysaccharides
EP98201312.0 1998-04-22
EP98201310.4 1998-04-22
EP98201311.2 1998-04-22
EP98201312A EP0957170A1 (en) 1998-04-22 1998-04-22 Identification of Lactobacillus delbrueckii subsp bulgaricus Lfi5 genes involved in the biosynthesis of exopolysaccharides

Publications (2)

Publication Number Publication Date
WO1999062316A2 WO1999062316A2 (en) 1999-12-09
WO1999062316A3 true WO1999062316A3 (en) 2000-08-17

Family

ID=27239317

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/EP1999/003011 Ceased WO1999054475A2 (en) 1998-04-22 1999-04-22 Identification of lactic acid bacterial genes involved in the biosynthesis of exopolysaccharides
PCT/EP1999/002841 Ceased WO1999062316A2 (en) 1998-04-22 1999-04-22 Identification of lactic acid bacteria genes involved in the biosynthesis of exopolysaccharides

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/003011 Ceased WO1999054475A2 (en) 1998-04-22 1999-04-22 Identification of lactic acid bacterial genes involved in the biosynthesis of exopolysaccharides

Country Status (2)

Country Link
AU (2) AU4604499A (en)
WO (2) WO1999054475A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001057234A2 (en) * 2000-02-02 2001-08-09 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University Biopolymer thickener
FR2807766A1 (en) * 2000-04-18 2001-10-19 Agronomique Inst Nat Rech OPERATE STREPTOCOCCUS THERMOPHILUS INVOLVED IN THE SYNTHESIS OF EPS
US6537786B2 (en) 2000-09-01 2003-03-25 E. I. Du Pont De Nemours And Company Genes encoding exopolysaccharide production
AU2007258872A1 (en) * 2006-06-16 2007-12-21 Danisco A/S Bacterium
WO2008053588A1 (en) * 2006-10-27 2008-05-08 Kabushiki Kaisha Yakult Honsha Cytokine production regulator gene and use thereof
CN108251348A (en) * 2018-03-29 2018-07-06 上海理工大学 A kind of preparation of thermophilus bacterium competence cell and method for transformation
MX2024006164A (en) * 2021-11-25 2024-06-11 Chr Hansen As POLYSACCHARIDES THAT RESULT IN IMPROVED WATER RETENTION CAPACITY OF DAIRY PRODUCTS.
WO2025233418A1 (en) * 2024-05-08 2025-11-13 International N&H Denmark Aps Strains of streptococcus thermophilus with enhanced texturing properties

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0699689A1 (en) * 1994-08-19 1996-03-06 Societe Des Produits Nestle S.A. Branched polysaccharide, microorganism producing it and compositions containing them
EP0750043A1 (en) * 1995-06-20 1996-12-27 Societe Des Produits Nestle S.A. Exopolysaccharides-producing lactic acid bacteria
EP0750042A1 (en) * 1995-06-20 1996-12-27 Societe Des Produits Nestle S.A. Exopolysaccarides-producing lactic acid bacteria

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0699689A1 (en) * 1994-08-19 1996-03-06 Societe Des Produits Nestle S.A. Branched polysaccharide, microorganism producing it and compositions containing them
NZ272795A (en) * 1994-08-19 1997-10-24 Nestle Sa Branched soluble polysaccharide whose main chain is made of repeating units of lactose; use in food, pharmaceutical and cosmetic compositions; microorganism for production thereof
EP0750043A1 (en) * 1995-06-20 1996-12-27 Societe Des Produits Nestle S.A. Exopolysaccharides-producing lactic acid bacteria
EP0750042A1 (en) * 1995-06-20 1996-12-27 Societe Des Produits Nestle S.A. Exopolysaccarides-producing lactic acid bacteria

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
GRIFFIN A M ET AL: "The cpsABCDE genes involved in polysaccharide production in Streptococcus salivarius ssp. thermophilus strain NCBF 2393", GENE, vol. 183, 1996, pages 23 - 27, XP004062722 *
GRUTER M ET AL: "Structural characterization of the exopolysaccharide produced by Lactobacillus delbrückii subspecies bulgaricus rr grown in skimmed milk", CARBOHYDRATE RESEARCH,NL,ELSEVIER SCIENTIFIC PUBLISHING COMPANY. AMSTERDAM, vol. 239, 1 January 1993 (1993-01-01), pages 209 - 226, XP002081314, ISSN: 0008-6215 *
LEMOINE J ET AL: "Structural characterisation of the exocellular polysaccharides produced by Streptococcus thermophilus SFi39 and SFi12.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 9, September 1997 (1997-09-01), pages 3512 - 3518, XP002080961 *
STINGELE F ET AL: "HOMOLOGOUS INTEGRATION AND TRANSPOSITION TO IDENTIFY GENES INVOLVED IN THE PRODUCTION OF EXOPOLYSACCHARIDES IN STREPTOCOCCUS THERMOPHILUS", DEVELOPMENTS IN BIOLOGICAL STANDARDIZATION, vol. 85, 1995, pages 487 - 493, XP000603799 *
STINGELE F ET AL: "IDENTIFICATION AND CHARACTERIZATION OF THE EPS (EXOPOLYSACCHARIDE) GENE CLUSTER FROM STREPTOCOCCUS THERMOPHILUS SFI6", JOURNAL OF BACTERIOLOGY, vol. 178, no. 6, March 1996 (1996-03-01), pages 1680 - 1690, XP000603803 *
STINGELE F ET AL: "Lactobacillus helveticus Lh59 secretes an exopolysaccharide that is identical to the one produced by Lactobacillus helveticus TN-4, a presumed spontaneous mutant of Lactobacillus helveticus TY1-2", CARBOHYDRATE RESEARCH,NL,ELSEVIER SCIENTIFIC PUBLISHING COMPANY. AMSTERDAM, vol. 302, no. 3-4, 7 August 1997 (1997-08-07), pages 197 - 202, XP004093562, ISSN: 0008-6215 *

Also Published As

Publication number Publication date
AU5969699A (en) 1999-12-20
WO1999054475A2 (en) 1999-10-28
AU4604499A (en) 1999-11-08
WO1999062316A2 (en) 1999-12-09

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