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WO1999061599A3 - Recombinant narbonolide polyketide synthase - Google Patents

Recombinant narbonolide polyketide synthase Download PDF

Info

Publication number
WO1999061599A3
WO1999061599A3 PCT/US1999/011814 US9911814W WO9961599A3 WO 1999061599 A3 WO1999061599 A3 WO 1999061599A3 US 9911814 W US9911814 W US 9911814W WO 9961599 A3 WO9961599 A3 WO 9961599A3
Authority
WO
WIPO (PCT)
Prior art keywords
polyketide synthase
narbonolide
recombinant
recombinant narbonolide
compounds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/011814
Other languages
French (fr)
Other versions
WO1999061599A2 (en
Inventor
Gary Ashley
Melanie C Betlach
Mary Betlach
Robert Mcdaniel
Li Tang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kosan Biosciences Inc
Original Assignee
Kosan Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/141,908 external-priority patent/US6503741B1/en
Application filed by Kosan Biosciences Inc filed Critical Kosan Biosciences Inc
Priority to EP99925954A priority Critical patent/EP1082439A2/en
Priority to CA002328427A priority patent/CA2328427A1/en
Priority to JP2000550984A priority patent/JP2002516090A/en
Priority to NZ509006A priority patent/NZ509006A/en
Priority to AU42137/99A priority patent/AU762399C/en
Publication of WO1999061599A2 publication Critical patent/WO1999061599A2/en
Publication of WO1999061599A3 publication Critical patent/WO1999061599A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Pyrane Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Recombinant DNA compounds that encode all or a portion of the narbonolide polyketide synthase are used to express recombinant polyketide synthase genes in host cells for the production of narbonolide, narbonolide derivatives, and polyketides that are useful as antibiotics and as intermediates in the synthesis of compounds with pharmaceutical value.
PCT/US1999/011814 1998-05-28 1999-05-27 Recombinant narbonolide polyketide synthase Ceased WO1999061599A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP99925954A EP1082439A2 (en) 1998-05-28 1999-05-27 Recombinant narbonolide polyketide synthase
CA002328427A CA2328427A1 (en) 1998-05-28 1999-05-27 Recombinant narbonolide polyketide synthase
JP2000550984A JP2002516090A (en) 1998-05-28 1999-05-27 Recombinant narvonolide polyketide synthase
NZ509006A NZ509006A (en) 1998-05-28 1999-05-27 Recombinant narbonolide polyketide synthase
AU42137/99A AU762399C (en) 1998-05-28 1999-05-27 Recombinant narbonolide polyketide synthase

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US8708098P 1998-05-28 1998-05-28
US60/087,080 1998-05-28
US09/141,908 US6503741B1 (en) 1998-05-28 1998-08-28 Polyketide synthase genes from Streptomyces venezuelae
US09/141,908 1998-08-28
US10088098P 1998-09-22 1998-09-22
US60/100,880 1998-09-22
US11913999P 1999-02-08 1999-02-08
US60/119,139 1999-02-08

Publications (2)

Publication Number Publication Date
WO1999061599A2 WO1999061599A2 (en) 1999-12-02
WO1999061599A3 true WO1999061599A3 (en) 2000-01-27

Family

ID=27492096

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/011814 Ceased WO1999061599A2 (en) 1998-05-28 1999-05-27 Recombinant narbonolide polyketide synthase

Country Status (6)

Country Link
EP (1) EP1082439A2 (en)
JP (1) JP2002516090A (en)
AU (1) AU762399C (en)
CA (1) CA2328427A1 (en)
NZ (1) NZ509006A (en)
WO (1) WO1999061599A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265202B1 (en) 1998-06-26 2001-07-24 Regents Of The University Of Minnesota DNA encoding methymycin and pikromycin
EP1754700A3 (en) 1999-01-27 2007-05-09 Kosan Biosciences, Inc. Synthesis of oligoketides
US7033818B2 (en) 1999-10-08 2006-04-25 Kosan Biosciences, Inc. Recombinant polyketide synthase genes
US6524841B1 (en) 1999-10-08 2003-02-25 Kosan Biosciences, Inc. Recombinant megalomicin biosynthetic genes and uses thereof
US6838265B2 (en) 2000-05-02 2005-01-04 Kosan Biosciences, Inc. Overproduction hosts for biosynthesis of polyketides
WO2002059322A2 (en) * 2000-10-17 2002-08-01 Cubist Pharmaceuticlas, Inc. Compositions and methods relating to the daptomycin biosynthetic gene cluster
ATE452896T1 (en) 2002-03-12 2010-01-15 Bristol Myers Squibb Co C3-CYANOEPOTHILONE DERIVATIVES
BR112013017884A2 (en) 2011-01-28 2019-09-24 Amyris Inc gel encapsulated microcolony scan
WO2012158466A1 (en) 2011-05-13 2012-11-22 Amyris, Inc. Methods and compositions for detecting microbial production of water-immiscible compounds
US10927382B2 (en) 2012-08-07 2021-02-23 Amyris, Inc. Methods for stabilizing production of acetyl-coenzyme a derived compounds
MY171958A (en) 2013-03-15 2019-11-08 Amyris Inc Use of phosphoketolase and phosphotransacetylase for production of acetyl-coenzyme a derived compounds
CA2918891C (en) 2013-08-07 2023-03-14 Amyris, Inc. Methods for stabilizing production of acetyl-coenzyme a derived compounds
WO2016210343A1 (en) 2015-06-25 2016-12-29 Amyris, Inc. Maltose dependent degrons, maltose-responsive promoters, stabilization constructs, and their use in production of non-catabolic compounds

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040968A1 (en) * 1995-06-07 1996-12-19 The Leland Stanford Junior University Recombinant production of novel polyketides
WO1998001546A2 (en) * 1996-07-05 1998-01-15 Biotica Technology Limited Polyketides and their synthesis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040968A1 (en) * 1995-06-07 1996-12-19 The Leland Stanford Junior University Recombinant production of novel polyketides
WO1998001546A2 (en) * 1996-07-05 1998-01-15 Biotica Technology Limited Polyketides and their synthesis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BROWN M J B ET AL: "A MUTANT GENERATED BY EXPRESSION OF AN ENGINEERED DEBS1 PROTEIN FROM THE ERYTHROMYCIN-PRODUCING POLYKETIDE SYNTHASE (PKS) IN STREPTOMYCES COELICOLOR PRODUCES THE TRIKETIDE AS A LACTONE, BUT THE MAJOR PRODUCT IS THE NOR-ANALOGUE DERIVED FROM ACETATE AS STARTER ACID", JOURNAL OF THE CHEMICAL SOCIETY, CHEMICAL COMMUNICATIONS,GB,CHEMICAL SOCIETY. LETCHWORTH, no. 15, 1995, pages 1517-1518, XP002044729, ISSN: 0022-4936 *

Also Published As

Publication number Publication date
NZ509006A (en) 2003-09-26
JP2002516090A (en) 2002-06-04
CA2328427A1 (en) 1999-12-02
WO1999061599A2 (en) 1999-12-02
AU4213799A (en) 1999-12-13
AU762399C (en) 2004-02-05
EP1082439A2 (en) 2001-03-14
AU762399B2 (en) 2003-06-26

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