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WO1999058699A3 - Transgenic plant-derived human blood coagulation factors - Google Patents

Transgenic plant-derived human blood coagulation factors Download PDF

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Publication number
WO1999058699A3
WO1999058699A3 PCT/US1999/010732 US9910732W WO9958699A3 WO 1999058699 A3 WO1999058699 A3 WO 1999058699A3 US 9910732 W US9910732 W US 9910732W WO 9958699 A3 WO9958699 A3 WO 9958699A3
Authority
WO
WIPO (PCT)
Prior art keywords
coagulation factors
plant
transgenic plant
human blood
derived human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/010732
Other languages
French (fr)
Other versions
WO1999058699A2 (en
Inventor
Brian S Hooker
Jianwei Gao
Daniel B Anderson
Ziyu Dai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Battelle Memorial Institute Inc
Original Assignee
Battelle Memorial Institute Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Battelle Memorial Institute Inc filed Critical Battelle Memorial Institute Inc
Priority to EP99921969A priority Critical patent/EP1076698A2/en
Priority to CA002328493A priority patent/CA2328493A1/en
Priority to JP2000548490A priority patent/JP2002514433A/en
Publication of WO1999058699A2 publication Critical patent/WO1999058699A2/en
Publication of WO1999058699A3 publication Critical patent/WO1999058699A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A composition is provided for transgenic plants and transgenic plant derived human coagulation factors capable of eliciting an activation response in human blood clotting pathways and therefore useful for the treatment of human beings diagnosed to be deficient in blood clotting factor proteins. Such proteins may be manufactured by methods resulting in viral free production using both whole plants and plant cell cultures. Also provided are expression vectors for the proper transformation of plant tissue for the production of such factors, as well as transformed plant cells and processes for producing human coagulation factors using plant molecular biology techniques.
PCT/US1999/010732 1998-05-14 1999-05-14 Transgenic plant-derived human blood coagulation factors Ceased WO1999058699A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99921969A EP1076698A2 (en) 1998-05-14 1999-05-14 Transgenic plant-derived human blood coagulation factors
CA002328493A CA2328493A1 (en) 1998-05-14 1999-05-14 Transgenic plant-derived human blood coagulation factors
JP2000548490A JP2002514433A (en) 1998-05-14 1999-05-14 Human clotting factor from transgenic plants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US8000398A 1998-05-14 1998-05-14
US30684799A 1999-05-06 1999-05-06
US09/306,847 1999-05-06
US09/080,003 1999-05-06

Publications (2)

Publication Number Publication Date
WO1999058699A2 WO1999058699A2 (en) 1999-11-18
WO1999058699A3 true WO1999058699A3 (en) 2000-04-06

Family

ID=26762686

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/010732 Ceased WO1999058699A2 (en) 1998-05-14 1999-05-14 Transgenic plant-derived human blood coagulation factors

Country Status (4)

Country Link
EP (1) EP1076698A2 (en)
JP (1) JP2002514433A (en)
CA (1) CA2328493A1 (en)
WO (1) WO1999058699A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2272648C2 (en) * 2000-05-10 2006-03-27 Ново Нордиск Хелт Кэр Аг Pharmaceutical composition comprising factor viia and factor xiii
ATE311198T1 (en) * 2000-05-10 2005-12-15 Novo Nordisk Healthcare Ag PHARMACEUTICAL COMPOSITION CONTAINING A FACTOR VIIA AND A TFPI INHIBITOR
ES2640613T3 (en) * 2000-07-21 2017-11-03 Revance Therapeutics, Inc. Multi-component biological agent transport systems
KR20040032147A (en) 2001-07-06 2004-04-14 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 Process for producing human thrombin by gene modification technique
US7125846B2 (en) 2001-11-09 2006-10-24 Novo Nordisk Healthcare A/G Pharmaceutical composition comprising factor VII polypeptides and factor V polypeptides
KR20050043734A (en) * 2001-11-09 2005-05-11 노보 노르디스크 헬스 케어 악티엔게젤샤프트 Pharmaceutical composition comprising factor vii polypeptides and factor v polypeptides
WO2005123928A1 (en) * 2004-06-08 2005-12-29 Battelle Memorial Institute Production of human coagulation factor viii from plant cells and whole plants
CN101014711A (en) 2004-06-25 2007-08-08 阿尔特生物科学公司 Production of tissue factor in plants
TW202417621A (en) * 2022-10-18 2024-05-01 博唯弘展生技股份有限公司 Production of recombinant human α-thrombin in plant-based system and the application thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0268772A2 (en) * 1986-09-19 1988-06-01 ZymoGenetics, Inc. Expression of biologically active factor XIII
WO1991002066A1 (en) * 1989-07-26 1991-02-21 Mogen International N.V. Production of heterologous proteins in plants and plant cells
WO1993013208A1 (en) * 1991-12-31 1993-07-08 Zymogenetics, Inc. Methods for producing thrombin
WO1994017415A1 (en) * 1993-01-29 1994-08-04 Dahlbaeck Bjoern Novel anticoagulant cofactor activity
US5723755A (en) * 1995-05-16 1998-03-03 Francis E. Lefaivre Large scale production of human or animal proteins using plant bioreactors

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0268772A2 (en) * 1986-09-19 1988-06-01 ZymoGenetics, Inc. Expression of biologically active factor XIII
WO1991002066A1 (en) * 1989-07-26 1991-02-21 Mogen International N.V. Production of heterologous proteins in plants and plant cells
WO1993013208A1 (en) * 1991-12-31 1993-07-08 Zymogenetics, Inc. Methods for producing thrombin
WO1994017415A1 (en) * 1993-01-29 1994-08-04 Dahlbaeck Bjoern Novel anticoagulant cofactor activity
US5723755A (en) * 1995-05-16 1998-03-03 Francis E. Lefaivre Large scale production of human or animal proteins using plant bioreactors

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CRAMER C L ET AL: "Bioproduction of human enzymes in transgenic tobacco.", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, (1996 MAY 25) 792 62-71. REF 50, XP000856387 *
DAI Z Y (REPRINT) ET AL: "Expression of human coagulation factor VIII gene in tobacco.", PLANT PHYSIOLOGY, (JUL 1997) VOL. 114, NO. 3, SUPP. [S], PP. 1584-1584. PUBLISHER: AMER SOC PLANT PHYSIOLOGISTS, 15501 MONONA DRIVE, ROCKVILLE, M 20855. ISSN: 0032-0889., BATTELLE MEM INST, PACIFIC NW LABS, BIOPROC GRP, RICHLAND, WA 99352, XP002123654 *
KANE W H ET AL: "Expression and characterization of recombinant human factor V and a mutant lacking a major portion of the connecting region.", BIOCHEMISTRY, (1990 JUL 24) 29 (29) 6762-8., XP002123653 *

Also Published As

Publication number Publication date
WO1999058699A2 (en) 1999-11-18
CA2328493A1 (en) 1999-11-18
EP1076698A2 (en) 2001-02-21
JP2002514433A (en) 2002-05-21

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