[go: up one dir, main page]

WO1999053037A3 - Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same - Google Patents

Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same Download PDF

Info

Publication number
WO1999053037A3
WO1999053037A3 PCT/US1999/008202 US9908202W WO9953037A3 WO 1999053037 A3 WO1999053037 A3 WO 1999053037A3 US 9908202 W US9908202 W US 9908202W WO 9953037 A3 WO9953037 A3 WO 9953037A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequence
methods
same
sequence encoding
expression
Prior art date
Application number
PCT/US1999/008202
Other languages
French (fr)
Other versions
WO1999053037A2 (en
Inventor
Joseph M Jilka
Original Assignee
Prodigene Inc
Joseph M Jilka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prodigene Inc, Joseph M Jilka filed Critical Prodigene Inc
Priority to AU40689/99A priority Critical patent/AU4068999A/en
Publication of WO1999053037A2 publication Critical patent/WO1999053037A2/en
Publication of WO1999053037A3 publication Critical patent/WO1999053037A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A nucleotide sequence is disclosed which is optimized for plant expression and which encodes organophosphorous hydrolase. Expression constructs, transformation vectors as well as transformed cells are also disclosed which achieve expression levels superior to those demonstrated by expression in bacteria such as E. coli for high throughput production of the enzyme. Transgenic plants may themselves be used or the protein may be harvested from said plants for in situ or other environmental detoxification of organophosphorous neurotoxin contaminated areas.
PCT/US1999/008202 1998-04-15 1999-04-14 Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same WO1999053037A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU40689/99A AU4068999A (en) 1998-04-15 1999-04-14 Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8184698P 1998-04-15 1998-04-15
US60/081,846 1998-04-15

Publications (2)

Publication Number Publication Date
WO1999053037A2 WO1999053037A2 (en) 1999-10-21
WO1999053037A3 true WO1999053037A3 (en) 1999-12-09

Family

ID=22166780

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/008202 WO1999053037A2 (en) 1998-04-15 1999-04-14 Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same

Country Status (2)

Country Link
AU (1) AU4068999A (en)
WO (1) WO1999053037A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6770799B2 (en) * 2000-03-17 2004-08-03 Thompson Boyce Plant Res Expression of recombinant human acetylcholinesterase in transgenic plants
AUPR502301A0 (en) 2001-05-15 2001-06-07 Commonwealth Scientific And Industrial Research Organisation Phosphotriesterase from Agrobacterium radiobacter P230
US20040109853A1 (en) 2002-09-09 2004-06-10 Reactive Surfaces, Ltd. Biological active coating components, coatings, and coated surfaces
AU2003303311A1 (en) * 2002-12-03 2004-08-30 Prodigene, Inc. Methods for selecting and screening for trasformants
CN1330760C (en) * 2005-10-11 2007-08-08 中国农业科学院生物技术研究所 Recombinant organophosphate degrading enzyme gene and its expression vector and prepn process
EP2195427A2 (en) 2007-10-01 2010-06-16 Guild Associates, Inc. Differentially fluorescent yeast biosensors for the detection and biodegradation of chemical agents
US8388904B1 (en) 2008-12-22 2013-03-05 Reactive Surfaces, Ltd., Llp Equipment decontamination system and method
CN102145218A (en) * 2010-11-24 2011-08-10 高旌 Rapid degradation agent for pesticide in plant
EP2734620A4 (en) 2011-07-20 2014-12-24 Commw Scient Ind Res Org ENZYMES FOR DEGRADING ORGANOSPHOSPHATES

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002177A1 (en) * 1988-08-26 1990-03-08 Amgen Inc. Parathion hydrolase analogs and methods for production and purification
EP0682115A1 (en) * 1988-09-09 1995-11-15 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5474925A (en) * 1991-12-19 1995-12-12 Agracetus, Inc. Immobilized proteins in cotton fiber

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002177A1 (en) * 1988-08-26 1990-03-08 Amgen Inc. Parathion hydrolase analogs and methods for production and purification
EP0682115A1 (en) * 1988-09-09 1995-11-15 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5474925A (en) * 1991-12-19 1995-12-12 Agracetus, Inc. Immobilized proteins in cotton fiber

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CUNNINGHAM S D ET AL: "PROMISES AND PROSPECTS OF PHYTOREMEDIATION", PLANT PHYSIOLOGY, vol. 110, 1 January 1996 (1996-01-01), pages 715 - 719, XP002051868, ISSN: 0032-0889 *
SALT D E ET AL: "PHYTOREMEDIATION: A NOVEL STRATEGY FOR THE REMOVAL OF TOXIC METALS FROM THE ENVIRONMENT USING PLANTS", BIO/TECHNOLOGY, vol. 13, no. 5, 1 May 1995 (1995-05-01), pages 468 - 474, XP000577130, ISSN: 0733-222X *
XU B ET AL.: "Enhanced Expression of a Bacterial Gene for Pesticide Degradation in a Common Soil Fungus", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 81, no. 6, 1996, pages 473 - 481, XP002112416 *

Also Published As

Publication number Publication date
AU4068999A (en) 1999-11-01
WO1999053037A2 (en) 1999-10-21

Similar Documents

Publication Publication Date Title
IL124020A (en) Plant optimized nucleotide sequence that encodes an insecticidal crystal protein
BR0306978A (en) isolated nucleic acid, expression vector, plant cell, plant, method for producing a transgenic plant, composition, methods for controlling plant growth, for stimulating stress tolerance of a plant, and for inhibiting growth of a transgenic plant, and use of a nucleic acid
BR0312064A (en) Genes encoding pesticidal proteins
WO2000005381A3 (en) Expression of enzymes involved in cellulose modification
WO1998027201A3 (en) Genes coding for amino acid deacetylases with specificity for n-acetyl-l-phosphinothricin, their isolation and their use
PL331767A1 (en) Transgenous plants expressing cellutitic enzymes
WO2001047952A3 (en) Insecticidal proteins from bacillus thuringiensis
CA2509100A1 (en) Plants having modified growth characteristics and a method for making the same
NZ334339A (en) A process of modifiying cellulose production, increasing stress resistance or increasing plant growth and yield in plants by inserting a DNA sequence encoding a product which modifies the level of cellulose precursors in the plant
ATE395427T1 (en) NEW ENZYMES THAT DEWATER GLYCERIN
Palmqvist et al. Characterisation of inorganic carbon fluxes, carbonic anhydrase (s) and ribulose-1, 5-biphosphate carboxylase-oxygenase in the green unicellular alga Coccomyxa: Comparisons with Iow-CO2 cells of Chlamydomonas reinhardtii
WO1999053037A3 (en) Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same
WO1999014331A3 (en) Cyclin-dependent kinase inhibitors and uses thereof
Ohshiro et al. Molecular cloning and nucleotide sequencing of organophosphorus insecticide hydrolase gene from Arthrobacter sp. strain B-5
WO2002086112A3 (en) Nucleic acid molecules encoding starch degrading enzymes
WO2006030445A3 (en) Isolated polypeptides and polynucleotides encoding same for generating plants with increased cuticlar water permeability
WO2002008432A3 (en) Composition and method for increased meiotic recombination in plants
AU6198000A (en) Gene encoding short integuments and uses thereof
CN102925419A (en) A mutant of organophosphorus pesticide degrading enzyme and its preparation method
WO2003020914A3 (en) Protein phosphatase stress-related polypeptides and methods of use in plants
PL342116A1 (en) Genetic method
WO1999013083A3 (en) Method and means for modulating plant cell cycle proteins and their use in controlling plant cell growth
CN103409387B (en) Organophosphorus pesticide degrading enzyme mutant and preparation method thereof
EP0959131A3 (en) Method for cloning and producing the AgeI restriction endonuclease in E. coli
CA2327350A1 (en) Bacillus thuringiensis toxins and genes for controlling coleopteran pests

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
NENP Non-entry into the national phase

Ref country code: KR

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase