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WO1999050659A3 - Rapid method for detecting micro-organisms and evaluating antimicrobial activity - Google Patents

Rapid method for detecting micro-organisms and evaluating antimicrobial activity Download PDF

Info

Publication number
WO1999050659A3
WO1999050659A3 PCT/GB1999/001001 GB9901001W WO9950659A3 WO 1999050659 A3 WO1999050659 A3 WO 1999050659A3 GB 9901001 W GB9901001 W GB 9901001W WO 9950659 A3 WO9950659 A3 WO 9950659A3
Authority
WO
WIPO (PCT)
Prior art keywords
organisms
micro
antimicrobial activity
rapid method
detecting micro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1999/001001
Other languages
French (fr)
Other versions
WO1999050659A2 (en
Inventor
Phillip Brown
Stuart Harbron
Gordon Chalmers Mellis Young
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zetatronics Ltd
Original Assignee
Zetatronics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9806759.8A external-priority patent/GB9806759D0/en
Priority claimed from GBGB9825222.4A external-priority patent/GB9825222D0/en
Application filed by Zetatronics Ltd filed Critical Zetatronics Ltd
Priority to AU33382/99A priority Critical patent/AU3338299A/en
Priority to EP99914648A priority patent/EP1068527A2/en
Priority to CA002326320A priority patent/CA2326320A1/en
Priority to JP2000541517A priority patent/JP2002510049A/en
Publication of WO1999050659A2 publication Critical patent/WO1999050659A2/en
Publication of WO1999050659A3 publication Critical patent/WO1999050659A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/80—Electrochemical detection via electrodes in contact with culture medium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method of identifying one or more micro-organisms in a fluid comprising the steps of: (i) taking a sample of the fluid containing a representative sample of any micro-organism(s) present; (ii) optionally culturing the sample if necessary to increase the number of micro-organisms for a pre-determined range; (iii) measuring the zeta potentials of any micro-organisms present; (iv) optionally normalising the measurements taken in step (iii) such that they relate to standard conditions; (v) comparing said measured zeta potentials with a table of the zeta potentials of known micro-organisms to determine which, if any, of the known micro-organisms are present in the fluid.
PCT/GB1999/001001 1998-03-31 1999-03-30 Rapid method for detecting micro-organisms and evaluating antimicrobial activity Ceased WO1999050659A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU33382/99A AU3338299A (en) 1998-03-31 1999-03-30 Rapid method for detecting micro-organisms and evaluating antimicrobial activity
EP99914648A EP1068527A2 (en) 1998-03-31 1999-03-30 Rapid method for detecting micro-organisms and evaluating antimicrobial activity
CA002326320A CA2326320A1 (en) 1998-03-31 1999-03-30 Rapid method for detecting micro-organisms and evaluating antimicrobial activity
JP2000541517A JP2002510049A (en) 1998-03-31 1999-03-30 A rapid method for detecting microorganisms and assessing antimicrobial activity

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB9806759.8 1998-03-31
GBGB9806759.8A GB9806759D0 (en) 1998-03-31 1998-03-31 Detection of micro-organisms
GBGB9820703.8A GB9820703D0 (en) 1998-03-31 1998-09-24 Improved method of detecting micro-organisms
GB9820703.8 1998-09-24
GBGB9825222.4A GB9825222D0 (en) 1998-11-19 1998-11-19 A rapid method for evaluating antimicrobial activity
GB9825222.4 1998-11-19

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09647572 A-371-Of-International 2001-04-02
US10/454,563 Continuation US20040067547A1 (en) 1998-03-31 2003-06-04 Rapid method for detecting micro-organisms and evaluating antimicrobial activity

Publications (2)

Publication Number Publication Date
WO1999050659A2 WO1999050659A2 (en) 1999-10-07
WO1999050659A3 true WO1999050659A3 (en) 2000-01-20

Family

ID=27269268

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/001001 Ceased WO1999050659A2 (en) 1998-03-31 1999-03-30 Rapid method for detecting micro-organisms and evaluating antimicrobial activity

Country Status (5)

Country Link
EP (1) EP1068527A2 (en)
JP (1) JP2002510049A (en)
AU (1) AU3338299A (en)
CA (1) CA2326320A1 (en)
WO (1) WO1999050659A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001061029A2 (en) * 2000-02-17 2001-08-23 Zetatronics Limited Identification method
GB0010628D0 (en) * 2000-05-04 2000-06-21 Zetatronics Ltd Process and apparatus for monitoring and controlling fermentation processes
US20100285140A1 (en) * 2007-12-27 2010-11-11 Shoichi Shirotake Antimicrobial agent for gram-positive bacteria
WO2012021802A2 (en) * 2010-08-12 2012-02-16 The Regents Of The University Of California Method for identifying antimicrobial compounds and determining antibiotic sensitivity

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3722504A (en) * 1969-12-23 1973-03-27 P Sawyer Method of screening substances for use in the treatment of circulatory system diseases
WO1979000834A1 (en) * 1978-03-24 1979-10-18 High Stoy Tech System for analyzing chemical and biological samples
WO1993007100A1 (en) * 1991-09-30 1993-04-15 Electric Power Research Institute, Inc. Use and selection of coating and surface materials to control surface fouling and corrosion using zeta potential measurement

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3722504A (en) * 1969-12-23 1973-03-27 P Sawyer Method of screening substances for use in the treatment of circulatory system diseases
WO1979000834A1 (en) * 1978-03-24 1979-10-18 High Stoy Tech System for analyzing chemical and biological samples
WO1993007100A1 (en) * 1991-09-30 1993-04-15 Electric Power Research Institute, Inc. Use and selection of coating and surface materials to control surface fouling and corrosion using zeta potential measurement

Also Published As

Publication number Publication date
EP1068527A2 (en) 2001-01-17
JP2002510049A (en) 2002-04-02
CA2326320A1 (en) 1999-10-07
WO1999050659A2 (en) 1999-10-07
AU3338299A (en) 1999-10-18

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