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WO1999049731A1 - Procedes et compositions destines a la desinfection d'objets sensibles a la chaleur - Google Patents

Procedes et compositions destines a la desinfection d'objets sensibles a la chaleur Download PDF

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Publication number
WO1999049731A1
WO1999049731A1 PCT/US1999/006652 US9906652W WO9949731A1 WO 1999049731 A1 WO1999049731 A1 WO 1999049731A1 US 9906652 W US9906652 W US 9906652W WO 9949731 A1 WO9949731 A1 WO 9949731A1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
weight
silver nitrate
alcohol
disinfecting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/006652
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English (en)
Inventor
Barnet R. Adelman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ADELMAN ASSOCIATES
Original Assignee
ADELMAN ASSOCIATES
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ADELMAN ASSOCIATES filed Critical ADELMAN ASSOCIATES
Priority to AU31170/99A priority Critical patent/AU3117099A/en
Publication of WO1999049731A1 publication Critical patent/WO1999049731A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof

Definitions

  • the invention relates generally to the field of sterilization. More particularly, the invention relates to the disinfection of heat-sensitive objects.
  • Chemical disinfection of heat sensitive objects has been used as an alternative to heat sterilization.
  • Chemicals used for disinfection include formaldehyde, glutaraldehyde, phenol and phenol derivatives, 50-70% alcohol in water, such as 70% ethanol, halogens, such as chlorine and iodine, heavy metals, such as mercuric chloride, silver nitrate and copper sulfate, ethylene oxide and detergents.
  • Desirable features of compositions for use in chemical sterilization are high toxicity to microorganisms, low toxicity to humans and animals, noncorrosiveness, stability and economy. Rarely does one chemical or chemical composition combine all of these features.
  • compositions and methods useful in the disinfection of objects, particularly heat sensitive objects are provided.
  • the compositions of the invention are disinfecting solutions comprising about 0.01-6% silver nitrate by weight and one
  • disinfecting solutions consisting essentially of one or more C -C 10 alcohol, about 0.01-6% silver nitrate by weight, 0-15%) water, 0-5% glycerin, and optionally, a perfume.
  • the methods of the invention comprise contacting an object to be disinfected with a disinfecting solution of the invention.
  • the invention comprises methods and compositions that can be used to disinfect objects.
  • the compositions of the invention are particularly suited for the disinfection of heat sensitive objects, and in particular, of heat-sensitive medical equipment.
  • heat sensitive objects include, but are not limited to, surgical instruments, thermometers, flexible endoscopes, lensed instruments, polyethylene tubing and catheters, and inhalation and anesthesia equipment.
  • the invention also methods of disinfecting objects through the use of these disinfecting solutions.
  • the invention has the additional advantage that the components of the disinfecting solutions are already approved for use with medical equipment and are non-corrosive to optics and/or electronics.
  • a synergistic killing effect has also been found for the combination of about 0.01- 6%> silver nitrate and about 0.2-10%) of a phenolic compound.
  • some of the most resistant pathogens are TB related because they are enclosed in polysaccharides.
  • the disinfecting solutions of the invention are particularly effective in killing TB related pathogens because the C - C 10 alcohol and/or the phenolic compound can dissolve the polysaccharide membrane, thereby exposing the interior portion of the pathogen to the silver nitrate.
  • the invention provides a disinfecting solution comprising about 0.01-6% silver nitrate by weight and one or more compound selected from the group consisting of: a C 2 -C ⁇ o alcohol and a phenolic compound.
  • the disinfecting solution may contain about OJ-6% silver nitrate by weight.
  • the disinfecting solution contains about 0.5-4%> silver nitrate by weight, more preferably about 1-2% silver nitrate by weight, and most preferably about 1.5% silver nitrate by weight.
  • Any C 2 -C 10 alcohol may be used in the disinfecting solutions of the invention.
  • alcohol is meant a compound in which a hydroxyl group is attached to a saturated carbon.
  • the saturated carbon may be a carbon of a simple alkyl group, such as ethanol, isopropanol, 4-methyl-l-pentanol, 3-chloro-l-l-propanol, 4-penten-2-ol and the like.
  • the carbon may be a saturated carbon of an alkenyl or alkynyl group, such as 2-propenol, 2-propynol and the like.
  • the carbon may be a saturated carbon that is attached to an aromatic ring, such as benzyl alcohol, phenyl alcohol and the like.
  • the alcohol may contain two hydroxyl groups, such as ethylene glycol, propylene glycol, trimethylene glycol and the like.
  • the C 2 -Cjo alcohol is ethanol, a propyl alcohol or a butyl alcohol.
  • the C 2 -C 10 alcohol is isopropanol.
  • the C 2 -C 10 alcohol can present in the disinfecting solutions of the invention at a concentration of about 60-99.5% ⁇ by weight.
  • concentration of C 2 -C 10 alcohol is about 80-98% by weight or 85-96% by weight. Even more preferably, the concentration of C 2 -C 10 alcohol is about 89-95% by weight or even 90-92%) by weight. Most preferably, the concentration of C 2 -C ⁇ o alcohol is about 90% by weight.
  • phenolic compound is meant a compound based on the substitution product of phenol.
  • Phenolic compounds include, but are not limited to, phenol, chlorophenol, nitrophenol, bromophenol, cresols, dihydroxybenzenes, phenylphenol, hexylresorcinol, hexachlorophene, methylphenol, ethylphenol, dimethylphenol, propylphenol, amylphenol, hexylphenol, heptylphenol and the like.
  • the phenolic compound is phenol.
  • the phenolic compound may be present in the disinfecting solutions of the invention at a concentration of about OJ-99% by weight.
  • the concentration of the disinfecting solutions of the invention may be present at a concentration of about OJ-99% by weight.
  • the concentration of the phenolic compound is about 0.2 to 10% by weight. Most preferably, the concentration of the phenolic compound is about 1-5% by weight. In a preferred embodiment, the phenolic compound is present at a concentration of about 2-5%. In one embodiment of the disinfection solution, one or more phenolic compound and/or one or more C 2 -C ⁇ 0 alcohol are combined with silver nitrate.
  • glycerin and perfume are useful additions in the disinfecting solutions of the invention.
  • Glycerin is helpful in dissolving the silver nitrate.
  • Glycerin may be present in the disinfecting solutions of the invention at a concentration of about 0-5% by weight, preferably at about 0.5-2%> by weight, most preferably at about 1% by weight.
  • Perfume aids in masking the odor of the C 2 -C ⁇ o alcohols and phenolic compounds having an offensive smell.
  • a perfume may be included in the disinfecting solutions of the invention at a concentration effective in partially or completely masking the offensive odor due to the C -C 10 alcohol and/or phenolic compound.
  • the invention provides a disinfecting solution consisting essentially of about 0.01-6%> silver nitrate by weight, a C -C 10 alcohol, 0-15%) water, 0-5%> glycerin and optionally, a perfume.
  • compositions of the invention are useful in disinfecting objects.
  • the invention is directed to a method of disinfecting an object comprising contacting said object with a disinfecting solution comprising about 0.01-6%) silver nitrate by weight and one or more compound selected from the group consisting of: C 2 -C 10 alcohols and phenolic compounds.
  • the object to be disinfected is exposed to the disinfecting solution of the invention.
  • the exposure of the object may be by immersion, dipping, submersion or spraying.
  • the exposure is for at least 10 minutes, more preferably at least 20 minutes, even more preferably at least 30 minutes, and most preferably, at least 40 minutes.
  • Ultrasonic stimulation of the articles and composition can be useful to expedite the sterilization process.
  • Preferred embodiments of the invention include exposing the object to the disinfecting solutions of the invention at a temperature of about 15-100°C. Even more preferably the temperature of said disinfecting solution is about 20-30°C.
  • the articles include exposing the articles to the disinfecting solution at 1 atmosphere.
  • the articles can be exposed to the disinfecting solution at lower or higher pressures (e.g., from approximately 0.5 to approximately 5.0 atmospheres).
  • the silver content of the composition can be at least in part recovered.
  • One way to recover the silver content of spent composition is distillation to obtain silver salts. The resulting silver salts can then be subsequently processed.
  • Test organisms Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and Mycobacterium terrae
  • Test organisms Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and Mycobacterium terrae
  • a 0J mL aliquot of each tested sample was transferred to a growth medium. After incubation, the samples were observed for growth. The absence of growth indicates antimicrobial activity of the disinfecting solution.
  • the Mycobacterium terrae ATCC #15755 culture was prepared by swabbing 7H11 agar plates with the organism. The plates were incubated at 37 ⁇ 2°C for 10 days. The agar plates were flooded with peptone tween (PEPT). A sterile bent glass rod was used to suspend the microbial growth. The suspension was added to a sterile container. A 1-2 mL aliquot of the culture suspension was added to a sterile tissue grinder with an equal volume of buffered gelatin. The suspension was macerated for at least 1 minute and then diluted in PEPT. A 10%o volume of glycerol was added to the diluted culture.
  • PEPT peptone tween
  • the suspension was pipetted into sterile 2 mL cryogenic vials.
  • the culture was then frozen at -10 to -30°C for 2 hours then placed into a -70°C freezer until needed.
  • a vial was removed from a -70°C freezer and thawed.
  • the thawed culture was diluted in a phosphate buffer to achieve an approximate concentration of 6 x 10 6 CFU/mL.
  • the titer of the test culture was confirmed using plate count techniques.
  • the Disinfecting solution was tested at four concentration levels. The concentration levels were: undiluted, 1/2, 1/4, and 1/8.
  • the dilutions of the disinfecting solution were made with sterile purified water. A control with purified water was also part of the test procedure.
  • One milliliter of each of the above dilutions was pipetted into a 13 x 100 mm sterile test tube and placed in a 20 ⁇ 1°C waterbath and equilibrated for ten minutes.
  • One set of dilution tubes was prepared for each of the four test organisms. A 100 ⁇ L volume of a bacterial solution was carefully pipetted into the test solutions and the purified water control. The tubes were then incubated in a waterbath at 20 ⁇ 1°C.
  • a 100 ⁇ L volume was removed from each of the dilutions of disinfecting solution at 30 and 60 minutes, and pipetted into a bottle containing sterile letheen broth. The contents of the bottles were filtered through a 0.45 ⁇ m membrane filter. A 100 mL aliquot of PEPT was then filtered to rinse the membrane. The membrane filter was then plated on 7H111 agar plates, and the 7H11 plates were incubated at 37 ⁇ 2°C for 21 days. After incubation, the plates were observed for growth.
  • a neutralization control was performed with each of the test organisms. If no growth was detected for an organism transferred to a growth medium after incubation with the disinfecting solution, the media was reinocculated with fresh, untreated cultures of the test microoganism. Growth of the test organism in the reinoculated medium ensured that the absence of original growth was due to the antimicrobial activity of the disinfecting solution, rather than to a defect in the growth medium.
  • a bottle containing the 100 mL of inoculated letheen growth medium was reinoculated with approximately 10-100 CFU of the appropriate test organism. A sample of the inoculum was plated to confirm the titer.
  • the bottles and plates were incubated at 37 ⁇ 2°C for 24 hours. After incubation the plates were counted and the bottles scored for growth.
  • the M. terrae neutralization control was performed by adding 0J mL of the undiluted test sample to 100 mL letheen broth. An additional bottle containing 100 mL letheen broth was included as a control. Each bottle was inoculated with approximately 10-100 CFU of M. terrae, filtered and plated on 7H11 agar as before. The plates were incubated at 37 ⁇ 2°C for 21 days. After the incubation period, the plates were counted. The percent recovery was then calculated.
  • the antimicrobial activity of the disinfecting solution is shown in Table 1. All tested organisms, including M. terrae, were inactivated after 30 minutes exposure to the undiluted disinfecting solution. The inoculating titer of each test organism is reported in Table 2. Neutralization control results are reported in Table 3.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Compositions et procédés utiles dans la désinfection d'objets, en particulier d'objets sensibles à la chaleur. Les compositions selon la présente invention contiennent d'environ 0,01 à 6 % en poids de nitrate d'argent et un ou plusieurs composés choisis dans le groupe constitué par les alcools C2-C10 et les composés phénoliques. La présente invention concerne également des compositions constituées essentiellement d'un ou plusieurs alcools C2-C10, d'environ 0,01 à 6 % en poids de nitrate d'argent, de 0 à 15 % en poids d'eau et de 0 à 5 % en poids de glycérine. Les procédés selon la présente invention consistent à mettre en contact un objet à désinfecter avec une composition décrite.
PCT/US1999/006652 1998-03-27 1999-03-26 Procedes et compositions destines a la desinfection d'objets sensibles a la chaleur Ceased WO1999049731A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU31170/99A AU3117099A (en) 1998-03-27 1999-03-26 Methods and compositions for disinfection of heat-sensitive objects

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7967398P 1998-03-27 1998-03-27
US60/79,673 1998-03-27

Publications (1)

Publication Number Publication Date
WO1999049731A1 true WO1999049731A1 (fr) 1999-10-07

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/006652 Ceased WO1999049731A1 (fr) 1998-03-27 1999-03-26 Procedes et compositions destines a la desinfection d'objets sensibles a la chaleur

Country Status (2)

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AU (1) AU3117099A (fr)
WO (1) WO1999049731A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2191163C1 (ru) * 2001-10-30 2002-10-20 Оганесов Владимир Емельянович Композиция для дезинфекции воды
EP1549145A4 (fr) * 2002-10-08 2006-07-26 Bradley J Eldred Systeme de purification et desinfection par la synergie de composes organiques et d'ions metalliques et procede de production

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04352702A (ja) * 1991-05-29 1992-12-07 Mitsubishi Motors Corp 防腐性処理液
WO1993016597A1 (fr) * 1992-02-26 1993-09-02 Henkel Kommanditgesellschaft Auf Aktien Produits germicides antiviraux

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04352702A (ja) * 1991-05-29 1992-12-07 Mitsubishi Motors Corp 防腐性処理液
WO1993016597A1 (fr) * 1992-02-26 1993-09-02 Henkel Kommanditgesellschaft Auf Aktien Produits germicides antiviraux

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 9303, Derwent World Patents Index; Class D22, AN 93-024046, XP002109134 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2191163C1 (ru) * 2001-10-30 2002-10-20 Оганесов Владимир Емельянович Композиция для дезинфекции воды
EP1549145A4 (fr) * 2002-10-08 2006-07-26 Bradley J Eldred Systeme de purification et desinfection par la synergie de composes organiques et d'ions metalliques et procede de production
US8173067B2 (en) 2002-10-08 2012-05-08 Bradley J. Eldred Organic compound and metal ion synergistic disinfection and purification system and method of manufacture

Also Published As

Publication number Publication date
AU3117099A (en) 1999-10-18

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