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WO1999047650A3 - PURIFIED RNA POLYMERASE FROM $i(ENTEROBACTER) - Google Patents

PURIFIED RNA POLYMERASE FROM $i(ENTEROBACTER) Download PDF

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Publication number
WO1999047650A3
WO1999047650A3 PCT/US1999/006158 US9906158W WO9947650A3 WO 1999047650 A3 WO1999047650 A3 WO 1999047650A3 US 9906158 W US9906158 W US 9906158W WO 9947650 A3 WO9947650 A3 WO 9947650A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna polymerase
enterobacter
purified rna
dna
purified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/006158
Other languages
French (fr)
Other versions
WO1999047650A2 (en
Inventor
Kelvin T Lam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scriptgen Pharmaceuticals Inc
Original Assignee
Scriptgen Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scriptgen Pharmaceuticals Inc filed Critical Scriptgen Pharmaceuticals Inc
Priority to AU33596/99A priority Critical patent/AU3359699A/en
Publication of WO1999047650A2 publication Critical patent/WO1999047650A2/en
Publication of WO1999047650A3 publication Critical patent/WO1999047650A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention provides a purified Enterobacter DNA-dependent RNA polymerase. In particular, RNA polymerase haloenzyme and core enzyme from E. cloacae are disclosed. The invention further provides methods of screening for antibacterial agents that bind to or modify the activity of an Enterobacter DNA-dependent RNA polymerase.
PCT/US1999/006158 1998-03-16 1999-03-16 PURIFIED RNA POLYMERASE FROM $i(ENTEROBACTER) Ceased WO1999047650A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU33596/99A AU3359699A (en) 1998-03-16 1999-03-16 Purified rna polymerase from (enterobacter)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7820398P 1998-03-16 1998-03-16
US60/078,203 1998-03-16

Publications (2)

Publication Number Publication Date
WO1999047650A2 WO1999047650A2 (en) 1999-09-23
WO1999047650A3 true WO1999047650A3 (en) 2000-01-20

Family

ID=22142591

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/006158 Ceased WO1999047650A2 (en) 1998-03-16 1999-03-16 PURIFIED RNA POLYMERASE FROM $i(ENTEROBACTER)

Country Status (2)

Country Link
AU (1) AU3359699A (en)
WO (1) WO1999047650A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114761555A (en) 2019-08-09 2022-07-15 胡桃钳医疗公司 Manufacturing method and apparatus for removing material from therapeutic compositions

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4693979A (en) * 1984-01-18 1987-09-15 Boehringer Mannheim Gmbh Type II restriction endonuclease Mae II, a process for obtaining it and the use thereof
WO1996025170A1 (en) * 1995-02-15 1996-08-22 Research Foundation Of State University Of New York METHOD FOR TREATMENT OF DISEASE WITH ANTI-SIGMA FACTOR AsiA
US5583026A (en) * 1994-08-31 1996-12-10 Cornell Research Foundation, Inc. Process for reconstituting the polymerase III* and other subassemblies of E. coli DNA polymerase III holoenzyme from peptide subunits

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4693979A (en) * 1984-01-18 1987-09-15 Boehringer Mannheim Gmbh Type II restriction endonuclease Mae II, a process for obtaining it and the use thereof
US5583026A (en) * 1994-08-31 1996-12-10 Cornell Research Foundation, Inc. Process for reconstituting the polymerase III* and other subassemblies of E. coli DNA polymerase III holoenzyme from peptide subunits
WO1996025170A1 (en) * 1995-02-15 1996-08-22 Research Foundation Of State University Of New York METHOD FOR TREATMENT OF DISEASE WITH ANTI-SIGMA FACTOR AsiA

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GAO N. ET AL.: "DNA-dependent RNA polymerase from Enterobacter cloacae is closely related to Escherichia coli", INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, vol. 29, no. 12, December 1997 (1997-12-01), pages 1485 - 1491, XP002117303 *

Also Published As

Publication number Publication date
WO1999047650A2 (en) 1999-09-23
AU3359699A (en) 1999-10-11

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