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WO1999045374A3 - Procedes de purification et de detection par electrophorese d'affinite reversible - Google Patents

Procedes de purification et de detection par electrophorese d'affinite reversible Download PDF

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Publication number
WO1999045374A3
WO1999045374A3 PCT/US1999/004849 US9904849W WO9945374A3 WO 1999045374 A3 WO1999045374 A3 WO 1999045374A3 US 9904849 W US9904849 W US 9904849W WO 9945374 A3 WO9945374 A3 WO 9945374A3
Authority
WO
WIPO (PCT)
Prior art keywords
purification
electrophoresis
detection processes
affinity electrophoresis
states
Prior art date
Application number
PCT/US1999/004849
Other languages
English (en)
Other versions
WO1999045374A2 (fr
Inventor
Ezra S Abrams
Philip W Hammond
Andrew R Muir
T Christian Boles
Original Assignee
Mosaic Technologies
Ezra S Abrams
Philip W Hammond
Andrew R Muir
T Christian Boles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mosaic Technologies, Ezra S Abrams, Philip W Hammond, Andrew R Muir, T Christian Boles filed Critical Mosaic Technologies
Priority to AU28963/99A priority Critical patent/AU2896399A/en
Priority to EP99909853A priority patent/EP1068518A2/fr
Priority to JP2000534862A priority patent/JP2002506204A/ja
Priority to CA002322975A priority patent/CA2322975A1/fr
Publication of WO1999045374A2 publication Critical patent/WO1999045374A2/fr
Publication of WO1999045374A3 publication Critical patent/WO1999045374A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Electrochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé d'électrophorèse d'affinité dans lequel on fait varier le sens de l'électrophorèse de manière cyclique tout en changeant de manière synchronisée une ou plusieurs propriétés du milieu électrophorétique entre deux états, lesdits états étant caractérisés comme favorisant ou défavorisant la liaison réversible spécifique de substances à analyser d'échantillons à des ligands d'affinité immobilisés dans le milieu. Le procédé obtenu permet une séparation extrêmement efficace et pratique des substances à analyser spécifiques à détecter ou à purifier, avec des matériels et un appareil simples.
PCT/US1999/004849 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible WO1999045374A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU28963/99A AU2896399A (en) 1998-03-03 1999-03-03 Purification and detection processes using reversible affinity electrophoresis
EP99909853A EP1068518A2 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible
JP2000534862A JP2002506204A (ja) 1998-03-03 1999-03-03 可逆的アフィニティー電気泳動を用いる精製および検出プロセス
CA002322975A CA2322975A1 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7661498P 1998-03-03 1998-03-03
US60/076,614 1998-03-03

Publications (2)

Publication Number Publication Date
WO1999045374A2 WO1999045374A2 (fr) 1999-09-10
WO1999045374A3 true WO1999045374A3 (fr) 2000-02-17

Family

ID=22133151

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/004849 WO1999045374A2 (fr) 1998-03-03 1999-03-03 Procedes de purification et de detection par electrophorese d'affinite reversible

Country Status (5)

Country Link
EP (1) EP1068518A2 (fr)
JP (1) JP2002506204A (fr)
AU (1) AU2896399A (fr)
CA (1) CA2322975A1 (fr)
WO (1) WO1999045374A2 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US7368233B2 (en) 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm
US8907058B2 (en) 2007-08-17 2014-12-09 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69835516D1 (de) 1997-05-16 2006-09-21 Exact Sciences Corp Elektrophoretische analyse von molekülen mit immobilisierten sonden
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
FR2799988B1 (fr) 1999-10-26 2001-12-07 Centre Nat Rech Scient Procede de separation d'un compose chimique ou biologique dans un melange de composes similaires par diffusion dans un milieu tel qu'un gel
FR2806161B1 (fr) 2000-03-10 2002-09-20 Sebia Sa Methode de separation, d'identification et de quantification des isophosphatases alcalines par electrophorese
JP4598989B2 (ja) * 2001-05-29 2010-12-15 アロカ株式会社 電気泳動装置
US7767468B2 (en) 2003-11-05 2010-08-03 Exact Sciences Corporation Repetitive affinity separation and uses therefor
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US8518228B2 (en) 2011-05-20 2013-08-27 The University Of British Columbia Systems and methods for enhanced SCODA
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
WO2005072854A1 (fr) 2004-02-02 2005-08-11 The University Of British Columbia Scodaphorese, procedes et appareil utilises pour deplacer et concentrer des particules
US7668235B2 (en) * 2005-11-10 2010-02-23 Teradyne Jitter measurement algorithm using locally in-order strobes
ES2635255T3 (es) 2006-08-29 2017-10-03 Becton Dickinson And Company Método y aparato para electroforesis de desviación libre de portador
WO2009094772A1 (fr) 2008-02-01 2009-08-06 The University Of British Columbia Procédés et appareil pour l'introduction et la récupération de particules
US8877028B2 (en) 2009-04-21 2014-11-04 The University Of British Columbia System and methods for detection of particles
US9951324B2 (en) 2010-02-25 2018-04-24 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using
WO2013104994A2 (fr) 2012-01-13 2013-07-18 The University Of British Columbia Appareil à bras multiples et procédés pour la séparation de particules
EP2844773B1 (fr) 2012-05-04 2017-08-16 Boreal Genomics Corp. Analyse de biomarqueurs utilisant la scodaphorèse
US9636413B2 (en) 2012-11-15 2017-05-02 Endocyte, Inc. Conjugates for treating diseases caused by PSMA expressing cells
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
DE202014011600U1 (de) 2013-10-18 2023-05-31 Deutsches Krebsforschungszentrum, Stiftung Des Öffentlichen Rechts Markierte Inhibitoren des prostataspezifischen Membranantigens (PSMA), deren Verwendung als bildgebende Mittel und pharmazeutische Wirkstoffe für die Behandlung von Prostatakrebs
JP6464166B2 (ja) 2013-11-14 2019-02-06 エンドサイト・インコーポレイテッドＥｎｄｏｃｙｔｅ， Ｉｎｃ． 陽電子放出断層撮影用の化合物
US10188759B2 (en) 2015-01-07 2019-01-29 Endocyte, Inc. Conjugates for imaging
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
CN112368024A (zh) 2018-04-17 2021-02-12 恩多塞特公司 治疗癌症的方法
CA3159566A1 (fr) 2019-10-29 2021-05-06 Quantum-Si Incorporated Pompage peristaltique de fluides et procedes, systemes et dispositifs associes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5167790A (en) * 1985-09-27 1992-12-01 Washington University Field-inversion gel electrophoresis
WO1996024687A1 (fr) * 1995-02-10 1996-08-15 University College London Detection de mutations ou de modifications dans l'adn
WO1997045721A1 (fr) * 1996-05-24 1997-12-04 Novartis Ag Procede pour separer des melanges de substances au moyen d'une lectrophorese sur gel par affinite capillaire

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5167790A (en) * 1985-09-27 1992-12-01 Washington University Field-inversion gel electrophoresis
WO1996024687A1 (fr) * 1995-02-10 1996-08-15 University College London Detection de mutations ou de modifications dans l'adn
WO1997045721A1 (fr) * 1996-05-24 1997-12-04 Novartis Ag Procede pour separer des melanges de substances au moyen d'une lectrophorese sur gel par affinite capillaire

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"BioDirectory Catalogue", 1997, PHARMACIA BIOTECH, XP002124683 *
PENNER ET AL.: "Increased detection of polymorphism among randomly amplified wheat DNA frgaments using a modified temperature sweep gel electrophoresis technique", NUCLEIC ACIDS RESEARCH, vol. 22, no. 9, 11 May 1994 (1994-05-11), pages 1780 - 1781, XP002124682 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US7368233B2 (en) 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm
US8907058B2 (en) 2007-08-17 2014-12-09 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using
US9193763B2 (en) 2007-08-17 2015-11-24 Purdue Research Foundation PSMA binding ligand-linker conjugates and methods for using

Also Published As

Publication number Publication date
WO1999045374A2 (fr) 1999-09-10
EP1068518A2 (fr) 2001-01-17
AU2896399A (en) 1999-09-20
CA2322975A1 (fr) 1999-09-10
JP2002506204A (ja) 2002-02-26

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