[go: up one dir, main page]

WO1998017780A1 - Micro-organisme du genre ascohyta et ses applications - Google Patents

Micro-organisme du genre ascohyta et ses applications Download PDF

Info

Publication number
WO1998017780A1
WO1998017780A1 PCT/JP1997/003511 JP9703511W WO9817780A1 WO 1998017780 A1 WO1998017780 A1 WO 1998017780A1 JP 9703511 W JP9703511 W JP 9703511W WO 9817780 A1 WO9817780 A1 WO 9817780A1
Authority
WO
WIPO (PCT)
Prior art keywords
strain
genus
ascokita
firefly
present
Prior art date
Application number
PCT/JP1997/003511
Other languages
English (en)
Japanese (ja)
Inventor
Masatoshi Gohbara
Michiyo Takabayashi
Original Assignee
Japan Tobacco Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc. filed Critical Japan Tobacco Inc.
Priority to KR1019980704543A priority Critical patent/KR100269417B1/ko
Priority to AU43983/97A priority patent/AU4398397A/en
Publication of WO1998017780A1 publication Critical patent/WO1998017780A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi

Definitions

  • the present invention relates to a novel strain belonging to the genus Ascochyta, and a method for controlling herbicides and weeds using the strain.
  • An object of the present invention is to provide a means for controlling weeds belonging to the genus Firefly in the family Calagidaceae using microorganisms. Disclosure of the invention
  • the present inventors have conducted intensive studies to solve the above problems, and as a result, have found that some strains belonging to the genus Ascokita have extremely excellent controllability against firefly weeds. And completed the present invention.
  • the present invention is a strain belonging to the genus Ascokita, which is pathogenic to any of the fireflies, fireflies, taiwanhamai, or squirrel fireflies.
  • the present invention is a herbicide comprising, as an active ingredient, a strain belonging to the genus Ascokita, which is pathogenic to any of the fireflies, fireflies, taiwanhamai, or squirrel fireflies.
  • the present invention is a method for controlling weeds using the above herbicide.
  • novel strain of the present invention ascokita sp. JT-1006, ascokita sp. JT-1007, ascokita sp. JT-1008, ascokita sp. JT-1009, and ascokita sp. JT-1010 will be described. I do. These strains were collected from diseased weeds of the genus Callarisaceae from all over Japan, isolated from strains having pathogenicity to the genus Firefly of the genus Callarisaceae, and purely cultured. It was created by screening means to examine the control effect and to examine the non-pathogenicity of other crops, especially rice.
  • the main types of mycological properties of the novel strain of the present invention are as follows.
  • the PH which is an aerobic bacterium and can grow is in the range of 3-13, preferably in the range of 5-7.
  • the growth temperature ranges from 15 to 30 degrees Celsius, and the optimum temperature for growth ranges from 25 to 30 degrees Celsius.
  • the growth on the potato dextrose agar medium is high, and the colony has a large number of orange-brown small grains radially and spirally in gray to olive-colored hyphae, and the number of aerial hyphae is low.
  • the conidia are colorless and similar to the genus Phoma, but have one partition in the center and are divided into two chambers, and are oval. The size of conidia is about 5-1 O imx about 2 _ 5 m.
  • Ascokita sp. JT-1006 strain FERM BP-5692 (deposit date October 3, 1996)
  • Ascokita sp. JT-1007 strain FERM BP-5693 (deposit date October 3, 1996)
  • any of a synthetic medium and a natural medium can be used as long as the medium appropriately contains assimilable carbon sources, nitrogen sources, inorganic substances, and necessary growth promoting substances.
  • Specific examples of the medium include potato dextrose agar medium (PDA) and Czapek agar medium.
  • This strain kills weeds belonging to the genus Callaridaceae in the paddy field or upland field, and does not affect crops such as eggplant, soybean, perilla, and cucumber, in addition to grasses such as rice, wheat, barley, and maize.
  • This strain can be cultivated in large quantities and can easily produce large quantities of spores.
  • Microorganisms used in the herbicide of the present invention include Ascokita sp. JT-1006 strain, Ascokita sp. JT-1007 strain, Ascokita sp. JT-1008 strain, Ascokita sp. JT-1009 strain, Ascokita sp. JT- Examples include 1010 strains, and mutants derived from these strains may be used.
  • the herbicide of the present invention comprises suspending spores obtained by mass-culturing the above strain in water.
  • the method of formulating a drug that can be prepared by the method is not limited to this. In this case, spore concentration, 1 0 6 -1 0 '2 / m 1 but is appropriate, but the invention is not restricted to this.
  • an auxiliary agent such as a surfactant and a spreading agent may be added.
  • Microorganisms which are the main agents, may be fresh ones immediately after culturing, or ones that have been once stored and reconstituted with water-based ones.
  • As the preservation method ultra-low-temperature preservation (180 ° C), vacuum freeze-drying and the like, which are widely known as preservation methods of microorganisms, can be used.
  • the control method of the present invention is carried out by spraying the herbicide on a field.
  • Application rate may be determined depending on the degree of overgrowth weeds, usually field 1 0 ares per spore amount 1 0 'preferably sprayed to 1 0 to be fifteen.
  • the herbicide and the plant to be controlled by the herbicide of the present invention are not particularly limited, but are particularly effective for plants belonging to the genus Firefly.
  • Examples of the firefly species mentioned here are (Scirpus juncoides var. ;), Sang-ri kui (Scirpus triquetula), Kang-ri "(Scirpus triangulatus) ⁇ cagara (Scirpus yagara), Kozuki-ya kara (Scirpus planiculmis), and the like, but are not limited thereto.
  • There is no particular limitation on the growth period of the plant to be controlled and the plant can be widely controlled from a plant shortly after germination to a grown plant.
  • the strain of the present invention was identified mainly by observing the conidial morphology. As a result, as described above, this strain was identified as a microorganism belonging to the genus Ascokita.
  • the microorganism of the present invention can easily and in large quantities obtain spores by culturing on a plate medium.
  • the obtained spores can be suspended in 10% skim milk and dried under vacuum to obtain a wettable powder.
  • This wettable powder can be suspended in water, added with a surfactant such as Tween 80, and applied.
  • Sugar beetle seeds are suspended in a solution obtained by adding an equal volume of 1% Tween 80 (Tween 80) solution to sodium hypochlorite solution (Sodium um hypochlor oride), and immersed for 1 to 3 minutes. Sterilized. Surface-sterilized seeds were sown on a medium in a test tube for plant growth which had been sterilized in advance and grown from the first to second leaf stage as test materials.
  • Tween 80 sodium hypochlorite solution
  • sodium um hypochlor oride sodium um hypochlor oride
  • the microorganism of the present invention exhibited an excellent control effect on the firefly (Sci rpus j unco i des var. Ohwi anus).
  • Tests on firefly weeds were performed in the same manner as in the virulence test on firefly, and two discs were used.
  • Test for crop performed in the same manner as virulence tests for Inuhotarui spore concentration of inoculum used was 1 0 7 / ml.
  • Test plants were rice, wheat, corn, eggplant, soybean, perilla, and cucumber. Table 13 shows the test results.
  • Conidia (10 '°) of Ascokita sp. JT-1008 strain and Twenty 80 (lg) were added to 1 L of water and mixed to prepare a liquid preparation.
  • the herbicide of the present invention can selectively kill weeds of the genus Fireflies of the family Calagidaceae and control their growth without affecting crops such as rice. In addition, it does not destroy the environment that pollutes the environment like chemical pesticides.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention a trait à des moyens permettant d'agir de manière sélective sur le dépérissement ou la croissance de plantes adventices du genre Scirpus de la famille du foin plat sans porter préjudice aux cultures, celle du riz par exemple, en utilisant des souches du genre Ascochyta faisant montre d'un pouvoir pathogène sur n'importe laquelle de ces plantes: S. juncoides, variété ohwianus, S. Juncoides, variété hotarui, S. wallichii ou S. lineolatus.
PCT/JP1997/003511 1996-10-17 1997-10-01 Micro-organisme du genre ascohyta et ses applications WO1998017780A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR1019980704543A KR100269417B1 (ko) 1996-10-17 1997-10-01 아스코키타속에 속하는 미생물 및 그 용도
AU43983/97A AU4398397A (en) 1996-10-17 1997-10-01 Microorganism of the genus (ascochyta) and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP8/274869 1996-10-17
JP8274869A JPH10117769A (ja) 1996-10-17 1996-10-17 アスコキタ属に属する微生物及びその用途

Publications (1)

Publication Number Publication Date
WO1998017780A1 true WO1998017780A1 (fr) 1998-04-30

Family

ID=17547703

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP1997/003511 WO1998017780A1 (fr) 1996-10-17 1997-10-01 Micro-organisme du genre ascohyta et ses applications

Country Status (6)

Country Link
JP (1) JPH10117769A (fr)
KR (1) KR100269417B1 (fr)
CN (1) CN1211276A (fr)
AU (1) AU4398397A (fr)
ID (1) ID18598A (fr)
WO (1) WO1998017780A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100769364B1 (ko) * 2006-04-12 2007-10-31 대한민국 설포닐우레아계 저항성 물달개비 방제용 포마 마크로스토마비더블유씨05-8-3-2 균주 및 이를 이용하여 제조되는균사체 현탁액

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0507039A1 (fr) * 1990-06-15 1992-10-07 Novo Nordisk A/S Composés à activité fongicide
JPH04368306A (ja) * 1991-06-18 1992-12-21 Japan Tobacco Inc 新規微生物、微生物を含有する除草剤およびそれらによる雑草の防除方法
WO1996024250A1 (fr) * 1995-02-08 1996-08-15 Novartis Ag Elimination biologique des mauvaises herbes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0507039A1 (fr) * 1990-06-15 1992-10-07 Novo Nordisk A/S Composés à activité fongicide
JPH04368306A (ja) * 1991-06-18 1992-12-21 Japan Tobacco Inc 新規微生物、微生物を含有する除草剤およびそれらによる雑草の防除方法
WO1996024250A1 (fr) * 1995-02-08 1996-08-15 Novartis Ag Elimination biologique des mauvaises herbes

Also Published As

Publication number Publication date
CN1211276A (zh) 1999-03-17
JPH10117769A (ja) 1998-05-12
KR19990072184A (ko) 1999-09-27
KR100269417B1 (ko) 2000-10-16
AU4398397A (en) 1998-05-15
ID18598A (id) 1998-04-23

Similar Documents

Publication Publication Date Title
RU2646138C1 (ru) Способ обработки семени растения
EP0756454B1 (fr) Composition et procede de lutte contre les maladies vegetales
MXPA03012034A (es) Nuevo biofungicida.
JP4090197B2 (ja) 微生物農薬
RU2313941C2 (ru) Препарат для защиты растений от возбудителей болезней сельскохозяйственных культур и винограда с ростстимулирующим эффектом, способ получения этого препарата и штаммы для его осуществления
JP2003531603A (ja) 新規のトリコデルマ属微生物菌株を利用した生物学的防除用微生物製剤およびその製造方法
JPH10506288A (ja) 植物の病気の生物学的防除のための真菌 Ｇｌｉｏｃｌａｄｉｕｍ ｃａｔｅｎｕｌａｔｕｍ
JP3132195B2 (ja) 新規微生物および植物病害防除剤
JPH06135811A (ja) 植物病害防除剤
WO1998017780A1 (fr) Micro-organisme du genre ascohyta et ses applications
US5902580A (en) Controlling Cyperus weeds with Ascochyta sp. FERM BP-5176
JPH0975070A (ja) アルタナリア属に属する微生物及びその用途
EP4331363A1 (fr) Activation des fonctions bionématicide et promotrice de la croissance végétale dans une solution biotechnologique
JP3073263B2 (ja) ドレックスレラ属の新菌株及びそれらを含有する雑草防除剤
WO2010064889A1 (fr) Composition pour contrôle biologique de maladies végétales
JPH0975067A (ja) シリンドロカルポン属に属する微生物及びその用途
JPH05284963A (ja) 新規微生物、該微生物を含有する除草剤及びそれらによる雑草の防除方法
JPH0975071A (ja) コレトトリカム属に属する微生物及びその用途
RU2588483C2 (ru) Штамм bradyrhizobium для улучшения роста растений (варианты), композиция, содержащая указанный штамм, и семя, покрытое композицией
JPH0975068A (ja) セプトリア属に属する微生物及びその用途
JPH1189563A (ja) エキセロヒラム属に属する微生物及びその用途
JP2002017343A (ja) イネ苗病害の防除剤および防除方法
JPH0912418A (ja) 雑草病原菌を用いたイネ科有用植物の病害防除剤及び防除方法
BRPI0009629B1 (pt) BIOCONTROLE DE DOENÇAS DE PLANTAS CAUSADAS POR ESPÉCIES DE Fusarium COM NOVOS ISOLADOS DE Bacillus megaterium

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 97192337.X

Country of ref document: CN

AK Designated states

Kind code of ref document: A1

Designated state(s): AU BR CN KR US

WWE Wipo information: entry into national phase

Ref document number: 1019980704543

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: 09091340

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 1019980704543

Country of ref document: KR

WWG Wipo information: grant in national office

Ref document number: 1019980704543

Country of ref document: KR