[go: up one dir, main page]

WO1998015293A3 - Targeted addition of soluble polymers to recombinant proteins - Google Patents

Targeted addition of soluble polymers to recombinant proteins Download PDF

Info

Publication number
WO1998015293A3
WO1998015293A3 PCT/US1997/018104 US9718104W WO9815293A3 WO 1998015293 A3 WO1998015293 A3 WO 1998015293A3 US 9718104 W US9718104 W US 9718104W WO 9815293 A3 WO9815293 A3 WO 9815293A3
Authority
WO
WIPO (PCT)
Prior art keywords
soluble polymers
recombinant proteins
targeted addition
protein
modification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/018104
Other languages
French (fr)
Other versions
WO1998015293A2 (en
Inventor
Brian Seed
Wen Shao
John Seed
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ADVANCED GENETIC TECHNOLOGY Corp
ADVANCED GENETIC TECHNOLOGY CO
Original Assignee
ADVANCED GENETIC TECHNOLOGY Corp
ADVANCED GENETIC TECHNOLOGY CO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ADVANCED GENETIC TECHNOLOGY Corp, ADVANCED GENETIC TECHNOLOGY CO filed Critical ADVANCED GENETIC TECHNOLOGY Corp
Priority to AU46722/97A priority Critical patent/AU4672297A/en
Priority to EP97945549A priority patent/EP1011730A2/en
Publication of WO1998015293A2 publication Critical patent/WO1998015293A2/en
Publication of WO1998015293A3 publication Critical patent/WO1998015293A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol

Landscapes

  • Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Proteins are modified with soluble polymers to change their biological properties, such as to render them less immunogenic and longer-lived in the body. The modification is by a strong, but non-covalent complexation between a metal chelate and an oligohistidine tag which is engineered into the protein. This method of protein modification leads to homogeneous preparations which can be readily prepared in large quantities.
PCT/US1997/018104 1996-10-07 1997-10-07 Targeted addition of soluble polymers to recombinant proteins Ceased WO1998015293A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU46722/97A AU4672297A (en) 1996-10-07 1997-10-07 Targeted addition of soluble polymers to recombinant proteins
EP97945549A EP1011730A2 (en) 1996-10-07 1997-10-07 Targeted addition of soluble polymers to recombinant proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2809196P 1996-10-07 1996-10-07
US60/028,091 1996-10-07

Publications (2)

Publication Number Publication Date
WO1998015293A2 WO1998015293A2 (en) 1998-04-16
WO1998015293A3 true WO1998015293A3 (en) 1998-06-18

Family

ID=21841531

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/018104 Ceased WO1998015293A2 (en) 1996-10-07 1997-10-07 Targeted addition of soluble polymers to recombinant proteins

Country Status (3)

Country Link
EP (1) EP1011730A2 (en)
AU (1) AU4672297A (en)
WO (1) WO1998015293A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6540897B1 (en) * 2000-07-13 2003-04-01 Pierce Chemical Company Direct detection of histidine tagged biomolecules on electrophoretic gel
US7045283B2 (en) 2000-10-18 2006-05-16 The Regents Of The University Of California Methods of high-throughput screening for internalizing antibodies
US20180028626A1 (en) 2015-02-13 2018-02-01 Transgene Sa Immunotherapeutic vaccine and antibody combination therapy
CN108586291A (en) * 2018-01-02 2018-09-28 成都傲飞生物化学品有限责任公司 A kind of N, N- are bis-（Carboxymethyl）The production technology of L-lysine

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993010233A1 (en) * 1991-11-11 1993-05-27 Fidia S.P.A. Synthesis and purification of truncated and mutein forms of human ciliary neuronotrophic factor
WO1995001797A1 (en) * 1993-07-09 1995-01-19 Smithkline Beecham Corporation Protein purification
WO1996009805A2 (en) * 1994-09-23 1996-04-04 Zonagen, Inc. Chitosan induced immunopotentiation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993010233A1 (en) * 1991-11-11 1993-05-27 Fidia S.P.A. Synthesis and purification of truncated and mutein forms of human ciliary neuronotrophic factor
WO1995001797A1 (en) * 1993-07-09 1995-01-19 Smithkline Beecham Corporation Protein purification
WO1996009805A2 (en) * 1994-09-23 1996-04-04 Zonagen, Inc. Chitosan induced immunopotentiation

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GERSHON P.D. ET AL: "Stable chelating linkage for reversible immobilization of oligohistidine tagged proteins in the BIAcore surface plasmon resonance detector", JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 183/1 SPEC. ISSUE (65-76), NETHERLANDS, XP004021026 *
HOCHULI E. ET AL.: "New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues", J. CHROMATOGRAPHY, vol. 411, 1987, pages 177 - 184, XP002059738 *
PORATH J. ET AL: "Metal chelate affinity chromatography, a new approach to protein fractionation", NATURE, vol. 258, 18 December 1975 (1975-12-18), pages 598 - 599, XP002059246 *

Also Published As

Publication number Publication date
AU4672297A (en) 1998-05-05
EP1011730A2 (en) 2000-06-28
WO1998015293A2 (en) 1998-04-16

Similar Documents

Publication Publication Date Title
IE892974L (en) Hydrophobic protein microparticles and preparation thereof
WO1990003123A3 (en) Hydrophobic protein microparticles and preparation thereof
CA2155185A1 (en) Totally synthetic affinity reagents
CA2331337A1 (en) Site specific protein modification by mutagenesis
WO1999029882A3 (en) Methods for altering the nutritional value of a plant protein, vegetative storage protein, vsp, by altering the amino acid content of proteins
AU9174398A (en) Peptides
IE802404L (en) Preparing modified whey proteins.
WO1993021331A3 (en) Polysaccharide binding fusion proteins and conjugates
AU6776600A (en) 2'-o-acetamido modified monomers and oligomers
PT2305819E (en) Conjugates prepared with aggregate-free proteins
EP1808180A3 (en) Modified GP 100 and uses thereof
CA2075974A1 (en) Totally synthetic affinity reagents
CA2195076A1 (en) Process for modifying the stability of antibodies
CA2307059A1 (en) Low viscosity meat emulsion and process for producing a protein composition useful to form a low viscosity meat emulsion
WO1993018146A3 (en) Proteins forming complexes with chaperones and ligands thereof, fragments thereof, preparation thereof and biological uses thereof
WO1997029199A3 (en) Prostate specific antigen peptides and uses thereof
WO1999037782A3 (en) Frozen food product
WO1998015293A3 (en) Targeted addition of soluble polymers to recombinant proteins
PT97383A (en) Process for the production of a globin chain, a binding fragment thereof and haemoglobin, and haemoglobin compositions
GB0010314D0 (en) Anti-freeze proteins their production and use
HK1044956A1 (en) Recombinant fusion molecules
WO1994025590A3 (en) Trichohyalin and transglutaminase-3 and methods of using same
DE60036555D1 (en) POLYPEPTIDE FRAGMENTS WITH A C-TERMINAL PART OF CATALASE FROM HELICOBACTER
US5830739A (en) Proteolytic mixture containing escharase and method of isolating same
WO2002034902A3 (en) Reversibly soluble enzyme-polymer conjugates

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH KE LS MW SD SZ UG ZW AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL

AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH KE LS MW SD SZ UG ZW AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 1997945549

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: CA

WWP Wipo information: published in national office

Ref document number: 1997945549

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1997945549

Country of ref document: EP