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WO1998004731A1 - Binary techniques for producing biologically active peptides - Google Patents

Binary techniques for producing biologically active peptides Download PDF

Info

Publication number
WO1998004731A1
WO1998004731A1 PCT/RU1996/000198 RU9600198W WO9804731A1 WO 1998004731 A1 WO1998004731 A1 WO 1998004731A1 RU 9600198 W RU9600198 W RU 9600198W WO 9804731 A1 WO9804731 A1 WO 9804731A1
Authority
WO
WIPO (PCT)
Prior art keywords
relates
biologically active
well
producing
active peptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/RU1996/000198
Other languages
French (fr)
Russian (ru)
Inventor
Alla Nikolaevna Maslikova
Viktor Nikolaevich Nechaev
Valery Ivanovich Fedchenko
Sergei Olegovich Guriev
Igor Gennadievich Sivov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
'nika-Universal' Firma
Original Assignee
'nika-Universal' Firma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 'nika-Universal' Firma filed Critical 'nika-Universal' Firma
Priority to AU11125/97A priority Critical patent/AU1112597A/en
Priority to PCT/RU1996/000198 priority patent/WO1998004731A1/en
Publication of WO1998004731A1 publication Critical patent/WO1998004731A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a method for producing biologically active peptides, wherein said method uses binary techniques for separately producing an active peptide precursor as well as a protease for processing said precursor. This invention also relates to a co-integration method of two replica using the repetitive sequence of a chromosome from bacteria of the genus Bordetella. This invention further relates to an expression system comprising a multi-copy plasmid with the target gene as well as a pT72 phasmid. The present invention finally relates to a method for producing annular forms of a T7 phage as well as to a method for preparing host cells/hyper-producers.
PCT/RU1996/000198 1996-07-25 1996-07-25 Binary techniques for producing biologically active peptides Ceased WO1998004731A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU11125/97A AU1112597A (en) 1996-07-25 1996-07-25 Binary techniques for producing biologically active peptides
PCT/RU1996/000198 WO1998004731A1 (en) 1996-07-25 1996-07-25 Binary techniques for producing biologically active peptides

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/RU1996/000198 WO1998004731A1 (en) 1996-07-25 1996-07-25 Binary techniques for producing biologically active peptides

Publications (1)

Publication Number Publication Date
WO1998004731A1 true WO1998004731A1 (en) 1998-02-05

Family

ID=20130016

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/RU1996/000198 Ceased WO1998004731A1 (en) 1996-07-25 1996-07-25 Binary techniques for producing biologically active peptides

Country Status (2)

Country Link
AU (1) AU1112597A (en)
WO (1) WO1998004731A1 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU559653A3 (en) * 1972-12-28 1977-05-25 Империал Кемикал Индастриз Лимитед (Фирма) The method of producing polypeptides
SU1149603A1 (en) * 1983-07-04 1985-11-23 Институт Экспериментальной Эндокринологии И Химии Гормонов Амн Ссср Polypeptide possessing hypoglycemic activity
US4686186A (en) * 1984-09-26 1987-08-11 Wisconsin Alumni Research Foundation Recombinant vector and eukaryotic host transformed thereby
SU1554766A3 (en) * 1980-07-24 1990-03-30 Карлсберг Биотекнолоджи, Лтд, А/С (Фирма) Method of producing human insulin of ins-thr-oh formula, or its derivatives
GB2253210A (en) * 1991-02-26 1992-09-02 Ici Plc Fermentation process for obtaining heterologous polypeptides
US5350682A (en) * 1990-06-13 1994-09-27 Hoechst Aktiengesellschaft Process for preparing peptides

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU559653A3 (en) * 1972-12-28 1977-05-25 Империал Кемикал Индастриз Лимитед (Фирма) The method of producing polypeptides
SU1554766A3 (en) * 1980-07-24 1990-03-30 Карлсберг Биотекнолоджи, Лтд, А/С (Фирма) Method of producing human insulin of ins-thr-oh formula, or its derivatives
SU1149603A1 (en) * 1983-07-04 1985-11-23 Институт Экспериментальной Эндокринологии И Химии Гормонов Амн Ссср Polypeptide possessing hypoglycemic activity
US4686186A (en) * 1984-09-26 1987-08-11 Wisconsin Alumni Research Foundation Recombinant vector and eukaryotic host transformed thereby
US5350682A (en) * 1990-06-13 1994-09-27 Hoechst Aktiengesellschaft Process for preparing peptides
GB2253210A (en) * 1991-02-26 1992-09-02 Ici Plc Fermentation process for obtaining heterologous polypeptides

Also Published As

Publication number Publication date
AU1112597A (en) 1998-02-20

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