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WO1997039120A3 - Antisense inhibitors of vascular endothelial growth factor (vefg/vpf) expression - Google Patents

Antisense inhibitors of vascular endothelial growth factor (vefg/vpf) expression Download PDF

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Publication number
WO1997039120A3
WO1997039120A3 PCT/US1997/006412 US9706412W WO9739120A3 WO 1997039120 A3 WO1997039120 A3 WO 1997039120A3 US 9706412 W US9706412 W US 9706412W WO 9739120 A3 WO9739120 A3 WO 9739120A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotides
expression
growth factor
vascular endothelial
endothelial growth
Prior art date
Application number
PCT/US1997/006412
Other languages
French (fr)
Other versions
WO1997039120A2 (en
Inventor
Nilabh Chaudhary
T Sudhakar Rao
Ganapathi R Revankar
Paul A Cossum
Robert F Rando
Anusch Peyman
Eugen Uhlmann
Original Assignee
Aronex Pharmaceuticals Inc
Nilabh Chaudhary
T Sudhakar Rao
Ganapathi R Revankar
Paul A Cossum
Robert F Rando
Anusch Peyman
Eugen Uhlmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aronex Pharmaceuticals Inc, Nilabh Chaudhary, T Sudhakar Rao, Ganapathi R Revankar, Paul A Cossum, Robert F Rando, Anusch Peyman, Eugen Uhlmann filed Critical Aronex Pharmaceuticals Inc
Priority to BR9708701-7A priority Critical patent/BR9708701A/en
Priority to JP9537367A priority patent/JP2000509259A/en
Priority to AU27336/97A priority patent/AU2733697A/en
Priority to EP97921238A priority patent/EP0910634A2/en
Publication of WO1997039120A2 publication Critical patent/WO1997039120A2/en
Publication of WO1997039120A3 publication Critical patent/WO1997039120A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/335—Modified T or U
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3515—Lipophilic moiety, e.g. cholesterol

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)

Abstract

The present invention relates to the inhibition of vascular endothelial growth factor expression with oligonucleotides. The oligonucleotides of the present invention are thought to bind to target nRNA in a sequence specific manner and prevent expression of the encoded gene. Chemical modifications of the oligonucleotides for increasing their stability and binding efficiency are disclosed. These modifications increase the stability and the efficiency of the oligonucleotides contemplated in this invention. Oligonucleotides compositions can be used in ex vivo therapies for the treatment of macrophages or in vivo therapies by injection, inhalation, topical treatment or other routes of administration.
PCT/US1997/006412 1996-04-17 1997-04-17 Antisense inhibitors of vascular endothelial growth factor (vefg/vpf) expression WO1997039120A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
BR9708701-7A BR9708701A (en) 1996-04-17 1997-04-17 Anti-sense inhibitors of vascular endothelial growth factor expression.
JP9537367A JP2000509259A (en) 1996-04-17 1997-04-17 Antisense inhibitor of vascular endothelial growth factor (VEgF / VPF) expression
AU27336/97A AU2733697A (en) 1996-04-17 1997-04-17 Antisense inhibitors of vascular endothelial growth factor (vegf/vpf) expression
EP97921238A EP0910634A2 (en) 1996-04-17 1997-04-17 ANTISENSE INHIBITORS OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEgF/VPF) EXPRESSION

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1575296P 1996-04-17 1996-04-17
US60/015,752 1996-04-17

Publications (2)

Publication Number Publication Date
WO1997039120A2 WO1997039120A2 (en) 1997-10-23
WO1997039120A3 true WO1997039120A3 (en) 1998-02-19

Family

ID=21773402

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/006412 WO1997039120A2 (en) 1996-04-17 1997-04-17 Antisense inhibitors of vascular endothelial growth factor (vefg/vpf) expression

Country Status (7)

Country Link
EP (1) EP0910634A2 (en)
JP (1) JP2000509259A (en)
KR (1) KR20000005561A (en)
AU (1) AU2733697A (en)
BR (1) BR9708701A (en)
CA (1) CA2251945A1 (en)
WO (1) WO1997039120A2 (en)

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US6858711B2 (en) 1996-01-23 2005-02-22 Affymetrix, Inc. Labeling reagents
US7291463B2 (en) 1996-01-23 2007-11-06 Affymetrix, Inc. Nucleic acid labeling compounds

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US20010018514A1 (en) 1998-07-31 2001-08-30 Mcgall Glenn H. Nucleic acid labeling compounds
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US7423143B2 (en) 1996-01-23 2008-09-09 Affymetrix. Inc. Nucleic acid labeling compounds
US7112338B2 (en) 1997-03-12 2006-09-26 The Regents Of The University Of California Cationic liposome delivery of taxanes to angiogenic blood vessels
US5837283A (en) 1997-03-12 1998-11-17 The Regents Of The University Of California Cationic lipid compositions targeting angiogenic endothelial cells
DE69919869T2 (en) 1998-06-10 2005-09-29 Biognostik Gesellschaft für Biomolekulare Diagnostik mbH STIMULATION OF THE IMMUNE SYSTEM
EP0978561A1 (en) * 1998-08-07 2000-02-09 Hoechst Marion Roussel Deutschland GmbH Antisense oligonucleotides for the inhibition of VEGF expression
JP2003520254A (en) * 2000-01-19 2003-07-02 エス． ジル，パーカッシュ Methods and compositions relating to antisense VEGF oligonucleotides
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WO2002072779A2 (en) 2001-03-12 2002-09-19 Affymetrix, Inc. Nucleic acid labeling compounds
WO2006006948A2 (en) 2002-11-14 2006-01-19 Dharmacon, Inc. METHODS AND COMPOSITIONS FOR SELECTING siRNA OF IMPROVED FUNCTIONALITY
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WO2007087113A2 (en) 2005-12-28 2007-08-02 The Scripps Research Institute Natural antisense and non-coding rna transcripts as drug targets
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US20110294870A1 (en) 2008-12-04 2011-12-01 Opko Curna, Llc Treatment of tumor suppressor gene related diseases by inhibition of natural antisense transcript to the gene
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US8940708B2 (en) 2009-12-23 2015-01-27 Curna, Inc. Treatment of hepatocyte growth factor (HGF) related diseases by inhibition of natural antisense transcript to HGF
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6858711B2 (en) 1996-01-23 2005-02-22 Affymetrix, Inc. Labeling reagents
US7291463B2 (en) 1996-01-23 2007-11-06 Affymetrix, Inc. Nucleic acid labeling compounds

Also Published As

Publication number Publication date
BR9708701A (en) 2000-01-04
AU2733697A (en) 1997-11-07
EP0910634A2 (en) 1999-04-28
WO1997039120A2 (en) 1997-10-23
JP2000509259A (en) 2000-07-25
CA2251945A1 (en) 1997-10-23
KR20000005561A (en) 2000-01-25

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