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WO1997037221A1 - Procede de marquage de molecules - Google Patents

Procede de marquage de molecules Download PDF

Info

Publication number
WO1997037221A1
WO1997037221A1 PCT/EP1997/001600 EP9701600W WO9737221A1 WO 1997037221 A1 WO1997037221 A1 WO 1997037221A1 EP 9701600 W EP9701600 W EP 9701600W WO 9737221 A1 WO9737221 A1 WO 9737221A1
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
labeled
matrix
reaction chamber
labeling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1997/001600
Other languages
German (de)
English (en)
Inventor
Bernd Schierholz
Günter J. BAUER
Irmgard Werner
Franz-Josef Meyer-Almes
Olivier Kreuzer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evotec Biosystems GmbH
Original Assignee
Evotec Biosystems GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec Biosystems GmbH filed Critical Evotec Biosystems GmbH
Priority to DE59707606T priority Critical patent/DE59707606D1/de
Priority to AT97916413T priority patent/ATE219837T1/de
Priority to EP97916413A priority patent/EP0907888B1/fr
Priority to US09/155,571 priority patent/US6140090A/en
Publication of WO1997037221A1 publication Critical patent/WO1997037221A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Definitions

  • Syringes filled with Texas Red ® Sufonylchloride-Celite ® were individually packed in Barr-O-Nit ® flat bags using a pulse welder and stored at -20 ° C. After 7, 13, 21, 27, 41, 76 and 455 days of storage, syringes were used to label alkaline phosphatase. The degrees of labeling were determined and compared with the achieved level of labeling of a syringe used directly after filling with Texas Red ® -sulfonyl chloride-Celite ® for labeling alkaline phosphatase.

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

L'invention concerne un procédé de marquage de molécules par réaction avec des marqueurs de molécules à marquer, sur une phase solide (matrice), caractérisé en ce que les molécules passent par un dispositif poreux pour parvenir à une chambre de réaction dans laquelle est disposée une matrice pourvue d'un composant réactif, et en ce que les molécules marquées, après couplage, quittent la chambre de réaction en passant par un dispositif poreux.
PCT/EP1997/001600 1996-04-02 1997-03-29 Procede de marquage de molecules Ceased WO1997037221A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE59707606T DE59707606D1 (de) 1996-04-02 1997-03-29 Verfahren zur markierung von molekülen
AT97916413T ATE219837T1 (de) 1996-04-02 1997-03-29 Verfahren zur markierung von molekülen
EP97916413A EP0907888B1 (fr) 1996-04-02 1997-03-29 Procede de marquage de molecules
US09/155,571 US6140090A (en) 1996-04-02 1997-03-29 Method for the labeling of molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19612650A DE19612650C1 (de) 1996-04-02 1996-04-02 Verfahren zur Fluoreszenzmarkierung von Molekülen
DE19612650.9 1996-04-02

Publications (1)

Publication Number Publication Date
WO1997037221A1 true WO1997037221A1 (fr) 1997-10-09

Family

ID=7789925

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1997/001600 Ceased WO1997037221A1 (fr) 1996-04-02 1997-03-29 Procede de marquage de molecules

Country Status (5)

Country Link
US (1) US6140090A (fr)
EP (1) EP0907888B1 (fr)
AT (1) ATE219837T1 (fr)
DE (2) DE19612650C1 (fr)
WO (1) WO1997037221A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ523831A (en) * 2000-07-13 2005-10-28 Invitrogen Corp Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix
WO2005012487A2 (fr) * 2003-08-01 2005-02-10 Invitrogen Corporation Compositions et procedes de preparation de courtes molecules d'arn et d'autres acides nucleiques

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4874813A (en) * 1987-02-09 1989-10-17 Shannessy Daniel J O Proteins bound to a marker or solid phase support matrix using a hydrazone linkage
DE3912046A1 (de) * 1988-09-02 1990-03-15 Univ Carnegie Mellon Verfahren zum markieren einer komponente einer waessrigen fluessigkeit
US5244816A (en) * 1989-10-11 1993-09-14 Akzo N.V. Method for purifying chelator conjugated compounds
US5290925A (en) * 1990-12-20 1994-03-01 Abbott Laboratories Methods, kits, and reactive supports for 3' labeling of oligonucleotides

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668476A (en) * 1984-03-23 1987-05-26 Applied Biosystems, Inc. Automated polypeptide synthesis apparatus
EP0356160A3 (fr) * 1988-08-24 1991-09-11 The Board Of Trustees Of The Leland Stanford Junior University Dispositif capillaire
GB8820256D0 (en) * 1988-08-26 1988-09-28 Coffey A F Continuous flow solid(gel)phase synthesis using intermittently packed & fluidised gel beds & layered displacement of reagent solutions & washing solvents
US5766933A (en) * 1989-04-26 1998-06-16 Diagnostic Products Corporation Method and element for measuring analytes in biological fluids using immobilized binder--analyte labeled complex
US5479937A (en) * 1989-09-21 1996-01-02 Epitope, Inc. Oral collection device
US5252496A (en) * 1989-12-18 1993-10-12 Princeton Biomeditech Corporation Carbon black immunochemical label
US5252296A (en) * 1990-05-15 1993-10-12 Chiron Corporation Method and apparatus for biopolymer synthesis
US5334538A (en) * 1990-11-26 1994-08-02 V-Tech, Inc. Gold sol immunoassay system and device

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4874813A (en) * 1987-02-09 1989-10-17 Shannessy Daniel J O Proteins bound to a marker or solid phase support matrix using a hydrazone linkage
DE3912046A1 (de) * 1988-09-02 1990-03-15 Univ Carnegie Mellon Verfahren zum markieren einer komponente einer waessrigen fluessigkeit
US5244816A (en) * 1989-10-11 1993-09-14 Akzo N.V. Method for purifying chelator conjugated compounds
US5290925A (en) * 1990-12-20 1994-03-01 Abbott Laboratories Methods, kits, and reactive supports for 3' labeling of oligonucleotides

Also Published As

Publication number Publication date
DE59707606D1 (de) 2002-08-01
ATE219837T1 (de) 2002-07-15
DE19612650C1 (de) 1997-07-31
EP0907888B1 (fr) 2002-06-26
US6140090A (en) 2000-10-31
EP0907888A1 (fr) 1999-04-14

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