WO1997012961B1 - Growth of adult pancreatic islet cells with laminin 5 - Google Patents
Growth of adult pancreatic islet cells with laminin 5Info
- Publication number
- WO1997012961B1 WO1997012961B1 PCT/US1996/015167 US9615167W WO9712961B1 WO 1997012961 B1 WO1997012961 B1 WO 1997012961B1 US 9615167 W US9615167 W US 9615167W WO 9712961 B1 WO9712961 B1 WO 9712961B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- iccs
- matrix
- cells
- laminin
- human
- Prior art date
Links
- 108010028309 kalinin Proteins 0.000 title claims abstract 11
- 210000004153 islets of langerhan Anatomy 0.000 title claims abstract 5
- 238000000034 method Methods 0.000 claims abstract 22
- 210000004027 cell Anatomy 0.000 claims abstract 13
- 239000011159 matrix material Substances 0.000 claims abstract 11
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims abstract 10
- 102000004877 Insulin Human genes 0.000 claims abstract 5
- 108090001061 Insulin Proteins 0.000 claims abstract 5
- 229940125396 insulin Drugs 0.000 claims abstract 5
- 210000002744 extracellular matrix Anatomy 0.000 claims abstract 3
- 206010005003 Bladder cancer Diseases 0.000 claims abstract 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims abstract 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims abstract 2
- 201000001531 bladder carcinoma Diseases 0.000 claims abstract 2
- 208000010570 urinary bladder carcinoma Diseases 0.000 claims abstract 2
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 claims 3
- 239000001963 growth medium Substances 0.000 claims 3
- 108020004511 Recombinant DNA Proteins 0.000 claims 2
- 238000002513 implantation Methods 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- HBOMLICNUCNMMY-KJFJCRTCSA-N 1-[(4s,5s)-4-azido-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O=C1NC(=O)C(C)=CN1C1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-KJFJCRTCSA-N 0.000 claims 1
- YTOPFCCWCSOHFV-UHFFFAOYSA-N 2,6-dimethyl-4-tridecylmorpholine Chemical compound CCCCCCCCCCCCCN1CC(C)OC(C)C1 YTOPFCCWCSOHFV-UHFFFAOYSA-N 0.000 claims 1
- 102100022744 Laminin subunit alpha-3 Human genes 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- 230000004888 barrier function Effects 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000002775 capsule Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 210000002919 epithelial cell Anatomy 0.000 claims 1
- 230000002349 favourable effect Effects 0.000 claims 1
- 210000000301 hemidesmosome Anatomy 0.000 claims 1
- 230000002480 immunoprotective effect Effects 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 210000003734 kidney Anatomy 0.000 claims 1
- 210000004185 liver Anatomy 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
Abstract
A method of increasing the number of adult pancreatic islet cells available for transplantation by contacting the cells with laminin 5 extracellular matrix. When contacted with the deposited matrix produced by 804G rat bladder carcinoma cells, a 1,500 fold increase in cell number is observed after three passages in culture. Islet cell expansion also occurs when cells are contacted with 804G soluble matrix. The expanded islet cells contain insulin and respond to glucose challenge.
Claims
1. A method of expanding adult pancreatic islet-like cell clusters (ICCs), comprising the steps of: culturing said ICCs in culture medium in contact with laminin 5; and passaging said cultured ICCs in contact with laminin 5 at least once, whereby the number of said cultured ICCs is significantly increased after said passaging.
2. The method of Claim 1, wherein said larninin 5 is the extracellular matrix produced by 804G or NBT-II rat bladder carcinoma cells.
3. The method of Claim 2, wherein said matrix comprises three polypeptides having molecular weights of about 150 kD, 140 kD, and 135 kD, said matrix characterized as:
(a) promoting enhanced growth of said ICCs in comparison to ICCs grown in the absence of the matrix;
(b) having the ability to promote hemidesmosome formation in epithelial cells cultured thereon;
(c) binding concanavalin; and
(d) being bound by polyclonal antibodies generated against the matrix.
4. The method of Claim 1, wherein said ICCs are from a mammal.
5. The method of Claim 4, wherein said ICCs are from a human.
6. The method of Claim 2, wherein said laminin 5 is deposited by said 804G or said NBT-II cells onto a substrate, said cells are removed from said matrix, and said ICCs are grown in contact with said matrix.
7. The method of Claim 2, wherein said matrix is secreted by said 804G or said NBT-II cells into the culture medium.
8. The method of Claim 7, further comprising purifying said secreted laminin 5 from said culture media.
9. The method of Claim 2, wherein said laminin 5 is produced by 804G cells.
10. The method of Claim 3, wherein said polypeptides are produced from recombinant DNA.
11. The method of Claim 10, wherein said recombinant DNA is human.
12. The method of Claim 1, wherein said laminin 5 is selected from the group consisting of kalinin and epiligrin. -15-
13. The method of Claim 1, wherein said laminin 5 is MCF 10A matrix.
14. The adult pancreatic ICCs prepared in accordance with Claim 1.
15. The method of Claim 1, wherein said ICCs are expanded at least 10 fold.
16. The method of Claim 1, wherein said ICCs are expanded at least 100 fold.
17. The method of Claim 1, wherein said ICCs are passaged at least twice, whereby functional insulin-producing passaged cells are obtained.
18. A method of treating Type I diabetes in a patient in need thereof, comprising the step of administering to said patient an effective insulin-producing amount of the ICCs of Claim 14.
19. The method of Claim 18, wherein said administering step is by implantation under the kidney capsule.
20. The method of Claim 18, wherein said ICCs are placed in an immunoprotective barrier prior to said implantation.
21. The method of Claim 18, wherein said administering step is by direct injection into the liver.
22. The method of Claim 18, wherein said effective insulin-producing amount is between about 2 x 105 and about 8 x 105 ICCs.
23. An effective insulin-producing amount of the ICCs of Claim 14 for use in treatment of Type I diabetes.
24. The ICCs of Claim 23, wherein said ICCs are human.
25. Use of the ICCs of Claim 14 in the preparation of a medicament for treatment of Type I diabetes.
26. The use of Claim 25, wherein said ICCs are human.
- 1 6-
STATEMENT UNDER ARTICLE 19
The claims have been amended to recite that adult ICCs are passaged at least once in culture. The cited references disclose growth, not passaging, of adult pancreatic islet cells on 804G matrix and other extracellular matrices. In view of the claim amendments, favorable international preliminary examination is respectfully requested.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT96935891T ATE255631T1 (en) | 1995-10-06 | 1996-09-23 | GROWTH OF ADULT PANCREAS LANGENHANS CELLS WITH LAMININ-5 |
| EP96935891A EP0859830B1 (en) | 1995-10-06 | 1996-09-23 | Growth of adult pancreatic islet cells with laminin 5 |
| AU73672/96A AU706581B2 (en) | 1995-10-06 | 1996-09-23 | Growth of adult pancreatic islet cells |
| JP9514294A JPH11513253A (en) | 1995-10-06 | 1996-09-23 | Growth of adult islet cells by laminin-5 |
| DE69630972T DE69630972T2 (en) | 1995-10-06 | 1996-09-23 | GROWTH OF ADULTS PANCREAS LANGENHANS CELLS WITH LAMININ-5 |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US487895P | 1995-10-06 | 1995-10-06 | |
| US60/004,878 | 1995-10-06 | ||
| US08/626,394 US5681587A (en) | 1995-10-06 | 1996-03-29 | Growth of adult pancreatic islet cells |
| US08/626,394 | 1996-03-29 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO1997012961A2 WO1997012961A2 (en) | 1997-04-10 |
| WO1997012961A3 WO1997012961A3 (en) | 1997-05-22 |
| WO1997012961B1 true WO1997012961B1 (en) | 1997-07-10 |
Family
ID=26673609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/015167 WO1997012961A2 (en) | 1995-10-06 | 1996-09-23 | Growth of adult pancreatic islet cells with laminin 5 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5681587A (en) |
| EP (1) | EP0859830B1 (en) |
| JP (1) | JPH11513253A (en) |
| AT (1) | ATE255631T1 (en) |
| AU (1) | AU706581B2 (en) |
| CA (1) | CA2233859A1 (en) |
| DE (1) | DE69630972T2 (en) |
| ES (1) | ES2211988T3 (en) |
| WO (1) | WO1997012961A2 (en) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6352707B1 (en) | 1992-02-24 | 2002-03-05 | Anton-Lewis Usala | Transplant encapsulation in a hydrogel matrix to obscure immune recognition |
| US6231881B1 (en) | 1992-02-24 | 2001-05-15 | Anton-Lewis Usala | Medium and matrix for long-term proliferation of cells |
| USRE36844E (en) * | 1993-04-05 | 2000-08-29 | Desmos Incorporated | Cellular attachment to trans-epithelial appliances |
| US5672361A (en) * | 1996-03-29 | 1997-09-30 | Desmos, Inc. | Laminin 5 for growth of pancreatic islet cells |
| US6294356B1 (en) | 1998-01-16 | 2001-09-25 | Northwestern University | Methods and materials for making and using laminin-5 |
| WO1999053955A1 (en) * | 1998-04-22 | 1999-10-28 | Desmos, Inc. | Creation of bioactive surfaces through selective adsorption |
| IL139883A0 (en) * | 1998-05-29 | 2002-02-10 | Cythera Inc | Promotion of cell differentiation by intially passaged cells |
| AU1093199A (en) * | 1998-10-13 | 2000-05-01 | General Hospital Corporation, The | Laminin 5, 13 and 14 and uses thereof |
| US6242666B1 (en) * | 1998-12-16 | 2001-06-05 | The Scripps Research Institute | Animal model for identifying a common stem/progenitor to liver cells and pancreatic cells |
| US6638765B1 (en) * | 1999-02-04 | 2003-10-28 | Mcgill University | Platform for the differentiation of cells |
| US6365385B1 (en) | 1999-03-22 | 2002-04-02 | Duke University | Methods of culturing and encapsulating pancreatic islet cells |
| US6303355B1 (en) | 1999-03-22 | 2001-10-16 | Duke University | Method of culturing, cryopreserving and encapsulating pancreatic islet cells |
| EP1177291A2 (en) | 1999-04-30 | 2002-02-06 | Biostratum, Inc. | Laminin 8 and methods for its use |
| AU4675300A (en) * | 1999-04-30 | 2000-11-17 | Biostatum, Inc. | Recombinant laminin 5 |
| JP2002542823A (en) | 1999-04-30 | 2002-12-17 | ユニヴァースティ オブ メディシン アンド デンティストリー オブ ニュージャージー ロバート ウッド ジョンソン メディカル スクール | Laminin 2 and how to use it |
| US6703363B1 (en) * | 1999-04-30 | 2004-03-09 | Biostratum, Inc. | Recombinant laminin 5 |
| WO2000078929A1 (en) * | 1999-06-23 | 2000-12-28 | Joslin Diabetes Center, Inc. | Methods of making pancreatic islet cells |
| US6815203B1 (en) | 1999-06-23 | 2004-11-09 | Joslin Diabetes Center, Inc. | Methods of making pancreatic islet cells |
| WO2001023528A1 (en) * | 1999-09-27 | 2001-04-05 | University Of Florida Research Foundation | Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures |
| CA2400355A1 (en) * | 2000-02-18 | 2001-08-23 | The Walter And Eliza Hall Institute Of Medical Research | Pancreatic islet cell growth factors |
| EP1814980B1 (en) * | 2004-11-22 | 2015-03-25 | Ramot at Tel-Aviv University Ltd. | Populations of expanded and re-differentiated adult islet beta cells capable of producing insulin and methods of generating same |
| US8735154B2 (en) * | 2006-10-30 | 2014-05-27 | The University Of Kansas | Templated islet cells and small islet cell clusters for diabetes treatment |
| US20080103606A1 (en) * | 2006-10-30 | 2008-05-01 | Cory Berkland | Templated islet cells and small islet cell clusters for diabetes treatment |
| US8895048B2 (en) * | 2010-04-06 | 2014-11-25 | The University Of Kansas | Templated islet cells and small islet cell clusters for diabetes treatment |
| CN111281890A (en) * | 2010-05-07 | 2020-06-16 | 北卡罗来纳大学查伯山分校 | Method for transplanting cells from solid tissues |
| KR101327630B1 (en) * | 2012-03-05 | 2013-11-13 | 울산대학교 산학협력단 | Method for manufacturing a carrier of transplanting pancreatic islet cells using atelocollagen, and artificial pancreas manufactured by the same method |
| WO2019098944A1 (en) * | 2017-11-15 | 2019-05-23 | National University Of Singapore | Systems for expanding pancreatic islets and transplantation thereof |
| CN113308433A (en) * | 2021-04-27 | 2021-08-27 | 立沃生物科技（深圳）有限公司 | Method for treating hybrid cells on surface of islet in-vitro culture |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0579742A4 (en) * | 1991-03-26 | 1994-07-13 | Oregon State | Product and method for improving keratinocyte adhesion to the dermis |
| ATE201994T1 (en) * | 1992-08-28 | 2001-06-15 | Oregon State | MEANS AND METHODS FOR IMPROVING KERATINOCYTE ADHESION TO THE SKIN |
| US5422264A (en) * | 1993-11-12 | 1995-06-06 | Desmos, Inc. | Soluble factor stimulation of attachment and hemidesmosome assembly in epithelial cells |
| US5541106A (en) * | 1993-04-05 | 1996-07-30 | Desmos, Inc. | Cell matrix stimulated attachment and hemidesmosome assembly |
| US5510263A (en) * | 1993-04-05 | 1996-04-23 | Desmos, Inc. | Growth of pancreatic islet-like cell clusters |
| AU7872894A (en) * | 1993-09-02 | 1995-03-22 | Fred Hutchinson Cancer Research Center | Epiligrin, an epithelial ligand for integrins |
| US5834308A (en) * | 1994-04-28 | 1998-11-10 | University Of Florida Research Foundation, Inc. | In vitro growth of functional islets of Langerhans |
-
1996
- 1996-03-29 US US08/626,394 patent/US5681587A/en not_active Expired - Fee Related
- 1996-09-23 AT AT96935891T patent/ATE255631T1/en not_active IP Right Cessation
- 1996-09-23 WO PCT/US1996/015167 patent/WO1997012961A2/en active IP Right Grant
- 1996-09-23 JP JP9514294A patent/JPH11513253A/en not_active Ceased
- 1996-09-23 CA CA002233859A patent/CA2233859A1/en not_active Abandoned
- 1996-09-23 AU AU73672/96A patent/AU706581B2/en not_active Ceased
- 1996-09-23 DE DE69630972T patent/DE69630972T2/en not_active Expired - Fee Related
- 1996-09-23 EP EP96935891A patent/EP0859830B1/en not_active Expired - Lifetime
- 1996-09-23 ES ES96935891T patent/ES2211988T3/en not_active Expired - Lifetime
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