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WO1997012241A1 - Procede et kit de biotest pour la caracterisation d'un support de production vegetale - Google Patents

Procede et kit de biotest pour la caracterisation d'un support de production vegetale Download PDF

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Publication number
WO1997012241A1
WO1997012241A1 PCT/CH1996/000328 CH9600328W WO9712241A1 WO 1997012241 A1 WO1997012241 A1 WO 1997012241A1 CH 9600328 W CH9600328 W CH 9600328W WO 9712241 A1 WO9712241 A1 WO 9712241A1
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WO
WIPO (PCT)
Prior art keywords
plant
individual
test
bioassays
biotest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CH1996/000328
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German (de)
English (en)
Inventor
Jacques Fuchs
Cornelia Fuchs-Truetsch
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GFF
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GFF
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Filing date
Publication date
Application filed by GFF filed Critical GFF
Priority to EP96930005A priority Critical patent/EP0796430A1/fr
Priority to AU69223/96A priority patent/AU6922396A/en
Publication of WO1997012241A1 publication Critical patent/WO1997012241A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G01N33/186—Water using one or more living organisms, e.g. a fish
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/24—Earth materials

Definitions

  • the present invention relates to a method for characterizing a plant production carrier with the aid of bioassays which can be carried out in a simple manner, and to bioassay kits for carrying out this method.
  • Linz substrate test a biological test of the plant compatibility of composts and potting soils', Gusenleitner J. et al., 1982, Bodenenkultur 33 (4): 305-313. This individual test provides information about the biological quality of composts and substrates. It is relatively complex to carry out, and that - 4 - plant production carrier,
  • test organisms are considered as test organisms that show a recognizable reaction in contact with the plant production carrier.
  • Test organisms which are selected from the group consisting of plants or their plant seeds, microorganisms, insects and worms are preferably used in the individual bioassays.
  • the materials which correspond to the types of plant production carriers which are usually to be examined are suitable as reference samples. These are preferably soil, compost, peat, peat substrates, rock wool and nutrient solutions.
  • Means for carrying out the individual bioassays include all the aids required for carrying out the bioassays, such as sample containers and cover foils, preferably plant containers, such as bowls and flower pots, and foils made of translucent plastics, such as polyethylene and polyester. Pots or bowls made of ceramic, glass or plastic can be used as sample containers. This also includes the descriptions and instructions for carrying out the individual bioassays.
  • Suitable means for the overall evaluation of the results of the individual bioassays are generally those that combine Responses to processes such as germination, growth, survival and the like.
  • Known bioassays can be used as individual bioassays, such as the Linz substrate test, the simple cress test, the water cress test, the pea test, the salad test, the closed cress test, the bean test, the red fescue test, the cucumber test, the tomato seedling test, the cabbage hernia test and the Chinese coal test.
  • bioassays based on the reaction of plants can be supplemented by further bioassays which are based on the reaction of other organisms, for example a bioassay based on the reaction of microorganisms, insects or worms, such as the Tubifex test or the Eisenia foetida test .
  • the individual bioassays are carried out in such a way that a reference sample is examined simultaneously with the sample to be examined, in which case the result obtained with the sample to be examined is evaluated in comparison with the result obtained with the reference sample.
  • the individual bioassays are preferably selected in such a way that the individual results obtainable with them add up to an overall statement which fully characterizes the particular sample of a plant production carrier examined in relation to a specific intended use. Preference is furthermore given to those bioassays which can be carried out with little expenditure on equipment and which are simple to carry out and evaluate and therefore require no scientifically trained personnel.
  • the number of individual tests to be carried out in individual cases is determined by the nature of the plant production carrier to be examined and the intended application.
  • a biotest kit according to the present invention contains:
  • the results of the individual tests must be compiled so that the overall evaluation can be carried out. This can happen, for example, if a few individual results are available by listing them in a simple table, or if there are a large number of individual results using a computer, which stores the individual results in the form of data series, data vectors, etc. and for further processing of the entirety of the individual results.
  • the gathering of individual results takes place in that the observer memorises them on the basis of the observation and evaluates the result of the bioassays directly without further aids.
  • the entirety of the individual results is now assessed in a further step.
  • This is preferably done with a simple expert system, which can be computerized with a large number of individual results.
  • Each test provides results which, by combining or coordinating with the results of the other tests, allow a meaningful assessment of the plant production vehicle.
  • the bioassays are selected so that each bioassay characterizes a different parameter of the plant production carrier, or that the entire reaction scale of the parameter to be characterized is covered thanks to the different sensitivity of the bioassays.
  • This creates a possibility for a comprehensive assessment of the quality and the biological compatibility of the plant production vehicle, a so-called quality profile.
  • a corresponding application profile is then also obtained, ie the expert system is able to provide individual test data in a clear manner.
  • Preferred means for the overall evaluation of the individual results are tables, pocket calculators, as well as computers and computer programs.
  • bioassays are carried out with the sample of a plant production vehicle, the quality of which is to be assessed, the individual tests preferably being selected so that their statements complement one another, so that an overall assessment of the plant production vehicle in relation to its intended application is possible is.
  • the number of bioassays used is not limited per se. In practice, however, no more bioassays will be carried out than are necessary for the complete characterization of the respective plant production carrier in relation to a particular intended use.
  • bioassays whose evaluation is simple, so that they can also be carried out by trained personnel.
  • bioassays are preferably used, the result of which can be assessed by simple observations (for example 'growing / not growing') or by simple measurements (for example weight measurement, length measurement).
  • Each test provides an individual result or information about one or, in individual cases, several properties of the plant production vehicle. However, these individual values are, by themselves, not sufficiently meaningful to assess the usability of the plant production vehicle for a specific application. Only the overall evaluation of the individual results provides a statement about the suitability of the plant production carrier for its use in The principle of the invention with regard to its areas of application is explained using four exemplary embodiments.
  • the first kit is described in detail, the other kits refer in part to the first.
  • Kit 1 Assessment of the compost quality and possible uses for the hobby gardener
  • This kit is equipped to carry out five different bioassays. Four of these are plant tolerance tests: simple cress test, salad test, and pea test (growth ability of cress, lettuce and peas in the compost compared to a reference substrate), the closed crescent test (growth of cress in the compost in an airtight glass jar) compared to a reference substrate). The fifth test is used to assess the presence or absence of the cabbage hernia pathogen. Cabbage hernia is known to be a very dangerous disease of the cabbage family.
  • the plant tolerance tests are differently sensitive to the compost quality, so that in comparison with reference samples with regard to the criterion 'plants do not grow / grow', each test is sufficient to assess the compost quality and its possible uses: is the result of the first test negative, the compost is of very poor quality and cannot be used; if this test is positive, but the others are negative, the compost can be used on shrubs, squashes and tomatoes; If the second test is also positive, it is generally possible to use the compost in the garden, except for sensitive plants such as beans, parsley, etc. if the result of the third test is also positive, the compost can be used in the whole garden; If the last plant tolerance test is also positive, the compost can also be used for growing the plants.
  • each individual element of the group is simple and, on its own, not very useful.
  • the combination of all elements results in a highly intelligent system with the ability to solve complicated questions.
  • the number and type of bio-test selection and evaluation scales for the design of bio-test kits can be varied as required. The design is based on the specified application options.
  • the users of this kit include, for example, plant producers in fruit, wine, vegetable and horticulture, in agriculture and forestry, hobby gardeners, consultants in areas of plant cultivation and ecology, substrate producers and manufacturers of fertilizers, pesticides and other auxiliaries of all kinds Type for plant production, experts in wastewater technology when examining the quality of wastewater and its possible uses; Industries and construction companies in assessing reuse of soil, assessing the impact of immissions on plant growth and ecology, side effects of products on plant production and ecology. Furthermore, this kit can be used in communities and authorities for problem solving and routine tests in the field of crop production, ecology and wastewater technology, as well as in schools and training organizations for practical exercises and demonstrations in the field of crop production and ecology. With this information, the usability of this bioassay is only indicated, but is in no way limited to these possible uses. - 10 - suggested. This kit is very interesting for the vegetable producer and provides him with valuable, inexpensive information. A similar kit is also available for agriculture.
  • Kit 4 Investigation of the suitability of an irrigation solution and its effect on soil biology and soil activity.
  • This test contains four vials of freeze-dried bacteria; 10 ml of reference solution are placed in two vials, and 5 ml of reference solution and 5 ml of irrigation solution are placed in each of the other two bottles.
  • the growth ability of the bacterium in these solutions is assessed visually after 1, 2, 3 and 5 days (as the bacterium grows, the solution becomes cloudy).
  • the overall evaluation can be carried out either with the help of a table or with a simple expert system on a computer.
  • the biological suitability of the tested irrigation solution for the various areas of application of the product can thus be determined easily, quickly and inexpensively. Recommendations for use, e.g. the necessary dilution factor of the irrigation solution is made if necessary.
  • the target audience of this kit are plant producers, as well as consultants and companies that offer auxiliaries for plant production, such as fertilizers, pesticides, etc.
  • Kit 2 Assessment of the possibilities of using compost for growing plants
  • Kit 1 This kit is aimed at professional compost producers and users. As for Kit 1, various plant tolerance tests are used, as well as a test for the weed-free nature of the compost. The selected plant tolerance tests are in some cases more sensitive than those described in Kit 1: open cress test, closed cress test, salad test, bean test and red fescue test. Plant weights are also collected for evaluation. With this kit you can easily and quickly predict how much compost can be used in seed mixes for which plant species. The overall evaluation can be done with the help of a table or with a simple expert system on a computer. The suitability and quality of commercially available seed soils can also be characterized.
  • Kit 3 Suitability of the soil quality of a field for problem crops in vegetable growing
  • the crops of various plants such as beans, peas, cabbage, etc. are difficult to grow in certain fields due to phytopathological problems.
  • this test can be used to predict which crop is possible or impossible in a field.
  • the soil samples to be examined are divided into two halves. One half is examined without addition, the other half is given glucose.
  • a cress, bean, pea, cucumber, tomato seedling and Chinese cabbage test are carried out on each half of the sample.
  • a water cress test is carried out by creating a water extract of the soil and observing the growth properties of the water cress.
  • the different plant tests show different (specific and non-specific) plant pathogens.
  • the overall evaluation determines the suitability of the field for the different crops and recommendations for the best cultivation methods - 12 - Claims:
  • a method for characterizing a plant production carrier with the aid of bioassays which can be carried out in a simple manner without great expenditure on apparatus, characterized in that a series of bioassays is carried out with a plant production carrier (step 1), that each individual bioassay is evaluated, whereby Individual results are determined (step 2) that the individual results are compiled into a total of individual results
  • Step 3 the entirety of the individual results are assessed (Step 4), which characterizes the plant production vehicle.
  • bioassay is observed and / or quantified via a reaction of plants or other organisms in contact with the plant production carrier or in contact with an extract of the plant production carrier.
  • characterized ge indicates that the characterization provides a quality profile and an application profile of the plant production vehicle.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Environmental & Geological Engineering (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Geology (AREA)
  • Remote Sensing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Fertilizers (AREA)

Abstract

Procédé et kit de biotest pour la caractérisation d'un support de production végétale, permettant de déterminer la qualité et la biocompatibilité de composts, substrats, terres, sols et substances auxiliaires de toutes sortes pour la production végétale. Des biotests simples sont effectués en parallèle dans des combinaisons sélectionnées (stade 1). L'évaluation de chacun des biotests est simple et n'exige aucune connaissance spéciale (stade 2). Les résultats respectifs des biotests sont rassemblés (stade 3). Leur évaluation globale est effectuée par un système d'experts (stade 4) et fournit un profil de qualité et un profil d'application pour les supports de production végétale concernés. On décrit des kits de biotest convenant pour l'évaluation de composts, substrats, terres, purins et substances auxiliaires de toutes sortes pour la production végétale. Des biotests de ce type trouvent leur application dans l'horticulture professionnelle ou non, dans la production végétale, dans l'industrie des engrais, dans la technologie des eaux résiduaires, dans l'agriculture, dans la viticulture et dans la sylviculture.
PCT/CH1996/000328 1995-09-25 1996-09-24 Procede et kit de biotest pour la caracterisation d'un support de production vegetale Ceased WO1997012241A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP96930005A EP0796430A1 (fr) 1995-09-25 1996-09-24 Procede et kit de biotest pour la caracterisation d'un support de production vegetale
AU69223/96A AU6922396A (en) 1995-09-25 1996-09-24 Method and biotest kit for characterizing a plant-production substrate

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH02695/95A CH689464A5 (de) 1995-09-25 1995-09-25 Verfahren und Biotest-Kit zur Charakterisierung eines Pflanzenproduktionstraegers.
CH2695/95-3 1995-09-25

Publications (1)

Publication Number Publication Date
WO1997012241A1 true WO1997012241A1 (fr) 1997-04-03

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PCT/CH1996/000328 Ceased WO1997012241A1 (fr) 1995-09-25 1996-09-24 Procede et kit de biotest pour la caracterisation d'un support de production vegetale

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EP (1) EP0796430A1 (fr)
AU (1) AU6922396A (fr)
CA (1) CA2205983A1 (fr)
CH (1) CH689464A5 (fr)
WO (1) WO1997012241A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100372361B1 (ko) * 2000-04-25 2003-02-15 한종필 토양 수분측정 시스템
PL422659A1 (pl) * 2017-08-28 2019-03-11 Mikronatura Środowiska Spółka Z Ograniczoną Odpowiedzialnością Mięczaki D. polymorpha i L. stagnalis do zastosowania w ocenie stanu ekologicznego zbiorników wodnych i rzek (wód i osadów dennych) oraz skuteczności działania substancji probiotycznych

Citations (7)

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Publication number Priority date Publication date Assignee Title
SU889701A1 (ru) * 1980-03-26 1981-12-15 Московский Ордена Ленина,Ордена Трудового Красного Знамени И Ордена Октябрьской Революции Государственный Университет Им.В.Ломоносова Способ определени микробного токсикоза почвы
SU900185A1 (ru) * 1980-03-17 1982-01-23 Украинский научно-исследовательский институт сельскохозяйственной микробиологии Способ определени фитотоксичности почвы
SU947761A1 (ru) * 1979-04-16 1982-07-30 Эстонский Научно-Исследовательский Институт Земледелия И Мелиорации Способ определени плодороди почвы
DE3726905C1 (en) * 1987-08-13 1988-10-06 Diagen Inst Molekularbio Method for detecting plant pathogens
WO1990012886A1 (fr) * 1989-04-26 1990-11-01 Hayes Kenneth R Procede et dispositif d'analyse biologique aquatique par fluorescence
DE4232281C1 (de) * 1992-09-26 1994-03-31 Meinhard Prof Dr Knoll Verfahren zur Bestimmung von Stoffkonzentrationen in Luft, Wasser oder Boden mit Hilfe lebender Pflanzen sowie Vorrichtungen zur Durchführung des Verfahrens
WO1995011989A1 (fr) * 1993-10-29 1995-05-04 Invitro International Dosage biologique in vitro concernant la toxicite d'un fluide

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU947761A1 (ru) * 1979-04-16 1982-07-30 Эстонский Научно-Исследовательский Институт Земледелия И Мелиорации Способ определени плодороди почвы
SU900185A1 (ru) * 1980-03-17 1982-01-23 Украинский научно-исследовательский институт сельскохозяйственной микробиологии Способ определени фитотоксичности почвы
SU889701A1 (ru) * 1980-03-26 1981-12-15 Московский Ордена Ленина,Ордена Трудового Красного Знамени И Ордена Октябрьской Революции Государственный Университет Им.В.Ломоносова Способ определени микробного токсикоза почвы
DE3726905C1 (en) * 1987-08-13 1988-10-06 Diagen Inst Molekularbio Method for detecting plant pathogens
WO1990012886A1 (fr) * 1989-04-26 1990-11-01 Hayes Kenneth R Procede et dispositif d'analyse biologique aquatique par fluorescence
DE4232281C1 (de) * 1992-09-26 1994-03-31 Meinhard Prof Dr Knoll Verfahren zur Bestimmung von Stoffkonzentrationen in Luft, Wasser oder Boden mit Hilfe lebender Pflanzen sowie Vorrichtungen zur Durchführung des Verfahrens
WO1995011989A1 (fr) * 1993-10-29 1995-05-04 Invitro International Dosage biologique in vitro concernant la toxicite d'un fluide

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* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 8242, Derwent World Patents Index; AN 82-89864E [42], XP002005089 *
DATABASE WPI Week 8247, Derwent World Patents Index; AN 82-A2151J [47], XP002005088 *
DATABASE WPI Week 8323, Derwent World Patents Index; AN 83-H7486K [23], XP002005087 *
GUSENLEITNER, J. ET AL.: "Linzer-Substrat-Test, eine biologische Prüfung der Pflanzenverträglichkeit von Komposten und Pflanzenerden", BODENKULTUR, vol. 33, no. 4, 1982, DE, pages 305 - 313, XP000572830 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100372361B1 (ko) * 2000-04-25 2003-02-15 한종필 토양 수분측정 시스템
PL422659A1 (pl) * 2017-08-28 2019-03-11 Mikronatura Środowiska Spółka Z Ograniczoną Odpowiedzialnością Mięczaki D. polymorpha i L. stagnalis do zastosowania w ocenie stanu ekologicznego zbiorników wodnych i rzek (wód i osadów dennych) oraz skuteczności działania substancji probiotycznych

Also Published As

Publication number Publication date
CA2205983A1 (fr) 1997-04-03
AU6922396A (en) 1997-04-17
CH689464A5 (de) 1999-04-30
EP0796430A1 (fr) 1997-09-24

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