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WO1997006268A3 - Constructions d'adn - Google Patents

Constructions d'adn Download PDF

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Publication number
WO1997006268A3
WO1997006268A3 PCT/GB1996/001846 GB9601846W WO9706268A3 WO 1997006268 A3 WO1997006268 A3 WO 1997006268A3 GB 9601846 W GB9601846 W GB 9601846W WO 9706268 A3 WO9706268 A3 WO 9706268A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
gene
insects
encodes
damaging
Prior art date
Application number
PCT/GB1996/001846
Other languages
English (en)
Other versions
WO1997006268A2 (fr
Inventor
Ian Jepson
Jacqueline Ann Mary Paine
Original Assignee
Zeneca Ltd
Ian Jepson
Jacqueline Ann Mary Paine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd, Ian Jepson, Jacqueline Ann Mary Paine filed Critical Zeneca Ltd
Priority to EP96925889A priority Critical patent/EP0846179A2/fr
Priority to APAP/P/1998/001194A priority patent/AP863A/en
Priority to NZ313724A priority patent/NZ313724A/en
Priority to CA002227445A priority patent/CA2227445A1/fr
Priority to MX9801008A priority patent/MX9801008A/es
Priority to JP9508208A priority patent/JPH11510694A/ja
Priority to PL96324880A priority patent/PL324880A1/xx
Priority to AU66252/96A priority patent/AU704172B2/en
Priority to SK169-98A priority patent/SK16998A3/sk
Priority to BR9609873A priority patent/BR9609873A/pt
Priority to IL12317296A priority patent/IL123172A0/xx
Publication of WO1997006268A2 publication Critical patent/WO1997006268A2/fr
Publication of WO1997006268A3 publication Critical patent/WO1997006268A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

On décrit une cassette d'expression de gènes de plantes pouvant être induite chimiquement, qui comprend un premier promoteur lié de manière fonctionnelle à une séquence de régulation, laquelle est dérivée du gène alcR et code une protéine régulatrice, ainsi qu'un promoteur pouvant être induit et qui est lié de manière fonctionnelle à un gène cible codant une protéine, laquelle est préjudiciable aux insectes, ou dont l'expression induit un processus métabolique produisant un métabolite, lequel est préjudiciable aux insectes. Le promoteur pouvant être induit est activé par la protéine régulatrice en présence d'un inducteur exogène efficace, une application de cet inducteur provoquant l'expression du gène cible.
PCT/GB1996/001846 1995-08-08 1996-07-29 Constructions d'adn WO1997006268A2 (fr)

Priority Applications (11)

Application Number Priority Date Filing Date Title
EP96925889A EP0846179A2 (fr) 1995-08-08 1996-07-29 Constructions d'adn
APAP/P/1998/001194A AP863A (en) 1995-08-08 1996-07-29 Dna constructs.
NZ313724A NZ313724A (en) 1995-08-08 1996-07-29 Dna constructs and promoter sequences and their use in the expression of genes which confer insecticidal activity on plants
CA002227445A CA2227445A1 (fr) 1995-08-08 1996-07-29 Constructions d'adn
MX9801008A MX9801008A (es) 1995-08-08 1996-07-29 Constructos de adn.
JP9508208A JPH11510694A (ja) 1995-08-08 1996-07-29 Ｄｎａ構築体
PL96324880A PL324880A1 (en) 1995-08-08 1996-07-29 Dna constructs
AU66252/96A AU704172B2 (en) 1995-08-08 1996-07-29 DNA constructs
SK169-98A SK16998A3 (en) 1995-08-08 1996-07-29 Chemically-inducible plant gene expression cassette
BR9609873A BR9609873A (pt) 1995-08-08 1996-07-29 Cassete de expressão de gene de planta quimicamente indutível célula de planta tecido de planta planta semente e processo par controlar insetos
IL12317296A IL123172A0 (en) 1995-08-08 1996-07-29 Dna constructs

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9516241.8A GB9516241D0 (en) 1995-08-08 1995-08-08 Dna constructs
GB9516241.8 1995-08-08

Publications (2)

Publication Number Publication Date
WO1997006268A2 WO1997006268A2 (fr) 1997-02-20
WO1997006268A3 true WO1997006268A3 (fr) 1997-03-13

Family

ID=10778942

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1996/001846 WO1997006268A2 (fr) 1995-08-08 1996-07-29 Constructions d'adn

Country Status (19)

Country Link
EP (1) EP0846179A2 (fr)
JP (1) JPH11510694A (fr)
KR (1) KR19990036251A (fr)
CN (1) CN1199425A (fr)
AP (1) AP863A (fr)
AR (1) AR002914A1 (fr)
AU (1) AU704172B2 (fr)
BR (1) BR9609873A (fr)
CA (1) CA2227445A1 (fr)
CZ (1) CZ36998A3 (fr)
GB (1) GB9516241D0 (fr)
HU (1) HUP9900057A3 (fr)
IL (1) IL123172A0 (fr)
MX (1) MX9801008A (fr)
NZ (1) NZ313724A (fr)
PL (1) PL324880A1 (fr)
SK (1) SK16998A3 (fr)
TR (1) TR199800177T1 (fr)
WO (1) WO1997006268A2 (fr)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
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GB9606062D0 (en) 1996-03-22 1996-05-22 Zeneca Ltd Promoters
GB9817704D0 (en) * 1998-08-13 1998-10-07 Zeneca Ltd Gene switch
GB9902236D0 (en) * 1999-02-01 1999-03-24 Zeneca Ltd Formulation
GB9902234D0 (en) * 1999-02-01 1999-03-24 Zeneca Ltd Expression system
GB9918154D0 (en) * 1999-08-02 1999-10-06 Zeneca Ltd Expression system
EP1925672A1 (fr) 2001-06-22 2008-05-28 Syngeta Participations AG Polynucléotides et polypeptides sensibles au stress abiotique
DE10212158A1 (de) 2002-03-19 2003-10-02 Metanomics Gmbh & Co Kgaa Population transgener Pflanzen, davon abgeleitetes biologisches Material, entsprechende Plasmidkollektion und Population transformierter Wirtsorganismen, sowie deren Verwendung und Verfahren zu deren Erzeugung
US20060117401A1 (en) 2002-12-20 2006-06-01 Oliver Schmitz Method for producing aminoacids
BRPI0407514A (pt) 2003-02-17 2006-02-14 Metanomics Gmbh método para preparar um organismo não humano, polinucleotìdeo, método para preparar um vetor, vetor, célula hospedeira, polipeptìdeo, anticorpo, ácido polinucléico de anti-sentido, método para preparar uma planta, célula vegetal, tecido vegetal transgênicos, célula de um animal útil, animal útil ou um microorganismo transgênico organismo não humano, microorganismo transgênico, rendimento ou um material de propagação de uma planta ou de um animal útil, biomassa de um microorganismo, método para preparar produtos quìmicos finos, e, uso de polipeptìdeo
CN1852985A (zh) 2003-08-01 2006-10-25 巴斯福植物科学有限公司 产生精细化学品的方法
WO2007087815A2 (fr) 2004-12-17 2007-08-09 Metanomics Gmbh Procédé de contrôle de production de produits chimiques fins
AU2005321630A1 (en) 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
CN101001956B (zh) 2004-07-31 2014-09-24 梅坦诺米克斯有限公司 制备具有更快生长和/或更高产量的生物
EP2194140A2 (fr) 2005-03-02 2010-06-09 Metanomics GmbH Procédé de production de produits chimiques fins
US20140199313A1 (en) 2005-03-02 2014-07-17 Metanomics Gmbh Process for the Production of Fine Chemicals
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CA2612116A1 (fr) 2005-07-06 2007-01-11 Cropdesign N.V. Amelioration du rendement de plantes par expression de gene de la famille ste20
EP1931788A2 (fr) 2005-09-15 2008-06-18 CropDesign N.V. Plantes a rendement accru et procede pour produire ces plantes
WO2007113237A2 (fr) 2006-03-31 2007-10-11 Basf Plant Science Gmbh Plantes ayant des caractéristiques de rendement de production accrues et leur procédé de fabrication
WO2008034648A1 (fr) 2006-04-05 2008-03-27 Metanomics Gmbh Procédé de production d'un produit chimique fin
BRPI0713097A2 (pt) 2006-05-31 2012-10-16 Metanomics Gmbh processo para a assimilação, acúmulo e/ou utilização de nitrogênio intensificados e/ou para o teor de nitrogênio total aumentado em um organismo fotossintético ativo, molécula de ácido nucleico isolada, construção de ácido nucleico, vetor, célula hospedeira, processo para produzir um polipeptìdeo, polipeptìdeo, anticorpo, tecido de planta, material de propagação, material colhido ou uma planta, método para a identificação de um produto de gene, composição, e, uso da molécula de ácido nucleico, do polipeptìdeo, da construção de ácido nucleico, do vetor, da planta ou tecido de planta, do material colhido da célula hospedeira ou do produto de gene identificado de acordo com o método
EP2035555A2 (fr) 2006-06-08 2009-03-18 BASF Plant Science GmbH Vegetaux aux caracteristiques des croissance ameliorees et procede d'obtention
CA2659414A1 (fr) 2006-08-02 2008-02-07 Cropdesign N.V. Se de polypeptide de translocation du sarcome synovial ameliorant le rendement des plantes soumises a un stress abiotique
ITUD20060280A1 (it) 2006-12-29 2008-06-30 Univ Degli Studi Udine Sequenza artificiale di dna evente funzione di leader in 5' (5'-utr) ottimizzata per la sovraespressione di proteine eterologhe in pianta
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US9816102B2 (en) 2012-09-13 2017-11-14 Indiana University Research And Technology Corporation Compositions and systems for conferring disease resistance in plants and methods of use thereof
EP3132028B1 (fr) 2014-04-17 2023-10-25 Boehringer Ingelheim RCV GmbH & Co KG Cellule hôte recombinée modifiée de sorte à surexprimer des protéines auxiliaires
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EP2952584A1 (fr) 2014-06-04 2015-12-09 Boehringer Ingelheim RCV GmbH & Co KG Production de protéines améliorée
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WO2016105696A1 (fr) 2014-12-23 2016-06-30 Syngenta Participations Ag Lutte biologique contre des coléoptères nuisibles
AU2016245959C1 (en) 2015-04-10 2021-05-27 Syngenta Participations Ag Animal feed compositions and methods of use
WO2018076335A1 (fr) 2016-10-31 2018-05-03 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Compositions et procédés pour améliorer la tolérance au stress abiotique
CN110612352B (zh) 2017-03-29 2025-01-24 贝林格尔·英格海姆Rcv两合公司 具有改变的膜脂组成的重组宿主细胞
EP3625340A4 (fr) 2017-05-18 2021-02-24 Cargill, Incorporated Système d'édition de génome
CN111148432B (zh) 2017-10-02 2021-11-23 先正达参股股份有限公司 工程化的杀有害生物蛋白和控制植物有害生物的方法
BR112020016306A2 (pt) 2018-02-12 2020-12-15 Curators Of The University Of Missouri Gene (saur) suprarregulado pequeno de auxina para o melhoramento da arquitetura do sistema radicular da planta, tolerância ao encharcamento, resistência à seca, e rendimento
CA3129325A1 (fr) 2019-02-20 2020-08-27 Syngenta Crop Protection Ag Proteines pesticides cry1 modifiees et methodes de controle des organismes nuisibles
JP2024506650A (ja) 2021-02-12 2024-02-14 ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 増加したタンパク質分泌のためのシグナルペプチド

Citations (4)

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Publication number Priority date Publication date Assignee Title
EP0159884A2 (fr) * 1984-04-13 1985-10-30 Lubrizol Genetics Inc. Promoteur et gène de choc thermique
WO1993007278A1 (fr) * 1991-10-04 1993-04-15 Ciba-Geigy Ag Sequence d'adn synthetique ayant une action insecticide accrue dans le mais
WO1993021334A1 (fr) * 1992-04-13 1993-10-28 Zeneca Limited Produits de recombinaison d'adn et plantes les incorporant
WO1995014098A1 (fr) * 1993-11-19 1995-05-26 Biotechnology Research And Development Corporation Regions regulatrices chimeres et cassettes de genes destines a l'expression de genes dans des plantes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0159884A2 (fr) * 1984-04-13 1985-10-30 Lubrizol Genetics Inc. Promoteur et gène de choc thermique
WO1993007278A1 (fr) * 1991-10-04 1993-04-15 Ciba-Geigy Ag Sequence d'adn synthetique ayant une action insecticide accrue dans le mais
WO1993021334A1 (fr) * 1992-04-13 1993-10-28 Zeneca Limited Produits de recombinaison d'adn et plantes les incorporant
WO1995014098A1 (fr) * 1993-11-19 1995-05-26 Biotechnology Research And Development Corporation Regions regulatrices chimeres et cassettes de genes destines a l'expression de genes dans des plantes

Non-Patent Citations (1)

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Title
LOCKINGTON, R.A. ET AL.: "Cloning and characterization of the ethanol utilization regulon in Aspergillus nidulans", GENE, vol. 33, 1985, pages 137 - 149, XP002022378 *

Also Published As

Publication number Publication date
JPH11510694A (ja) 1999-09-21
BR9609873A (pt) 1999-03-23
WO1997006268A2 (fr) 1997-02-20
IL123172A0 (en) 1998-09-24
AU704172B2 (en) 1999-04-15
PL324880A1 (en) 1998-06-22
MX9801008A (es) 1998-04-30
AU6625296A (en) 1997-03-05
EP0846179A2 (fr) 1998-06-10
HUP9900057A3 (en) 2001-06-28
AR002914A1 (es) 1998-04-29
CZ36998A3 (cs) 1998-06-17
TR199800177T1 (xx) 1998-05-21
HUP9900057A2 (hu) 1999-04-28
AP863A (en) 2000-08-04
CN1199425A (zh) 1998-11-18
KR19990036251A (ko) 1999-05-25
GB9516241D0 (en) 1995-10-11
CA2227445A1 (fr) 1997-02-20
AP9801194A0 (en) 1998-03-31
NZ313724A (en) 1999-04-29
SK16998A3 (en) 1998-09-09

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