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WO1997004126B1 - Method for amplifying nucleic acid sequences by translocation using chimeric primers - Google Patents

Method for amplifying nucleic acid sequences by translocation using chimeric primers

Info

Publication number
WO1997004126B1
WO1997004126B1 PCT/FR1996/001166 FR9601166W WO9704126B1 WO 1997004126 B1 WO1997004126 B1 WO 1997004126B1 FR 9601166 W FR9601166 W FR 9601166W WO 9704126 B1 WO9704126 B1 WO 9704126B1
Authority
WO
WIPO (PCT)
Prior art keywords
dna
segment
rna
type
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR1996/001166
Other languages
French (fr)
Other versions
WO1997004126A1 (en
Filing date
Publication date
Priority claimed from FR9508945A external-priority patent/FR2737223B1/en
Application filed filed Critical
Priority to CA2200627A priority Critical patent/CA2200627C/en
Priority to EP96926456A priority patent/EP0787209B1/en
Priority to AT96926456T priority patent/ATE214741T1/en
Priority to US08/817,035 priority patent/US5824517A/en
Priority to DE69619959T priority patent/DE69619959T2/en
Publication of WO1997004126A1 publication Critical patent/WO1997004126A1/en
Publication of WO1997004126B1 publication Critical patent/WO1997004126B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

A target nucleic acid sequence (12) may be amplified in the presence of an enzymatic system including DNA polymerase, strand translocation and RNAse H activities by using a chimeric primer (A1, A2) that includes, in the 5' to 3' direction, an RNA-type segment capable of hybridising with a 3'-terminal segment of the target and a DNA-type segment capable of hybridising with a segment adjacent to said 3'-terminal segment of the target, and a DNA- or RNA-type primer B1 capable of hybridising with said 3'-terminal segment of the target. A cyclic amplification is achieved that may be implemented isothermally on the basis of either a DNA or an RNA target even when the terminals are not defined.

Claims

REVENDICATIONS MODIFIEES MODIFIED CLAIMS [reçues par le Bureau international le 22 janvier 1997 (22.01.97); revendication 8 modifiée; autres revendications inchangées (1 page)][received by the International Bureau on 22 January 1997 (22.01.97); amended claim 8; other claims unchanged (1 page)] polynucléotidique amont.upstream polynucleotide. 7. Procédé selon l'une quelconque des revendications 1 à 4, caractérisé par le fait que le produit de départ est un ARN contenant la séquence à amplifier, et que pour obtenir ledit polynucléotide monobrin de type ADN, on met en contact ledit ARN avec lesdites première et seconde amorces et le cas échéant avec lesdites troisième et quatrième amorces.7. Method according to any one of claims 1 to 4, characterized in that the starting material is an RNA containing the sequence to be amplified, and that to obtain said DNA-type single-stranded polynucleotide, said RNA is contacted with said first and second primers and where appropriate with said third and fourth primers. 8. Nécessaire pour la mise en oeuvre d'un procédé d'amplification d'une séquence d'acide nucléique cible, caractérisé par le fait qu'il comprend un système enzymatique ayant une activité de RNAse H et au moins une amorce chimère comprenant successivement, dans le sens 5'→ 3':8. Necessary for carrying out a method of amplification of a target nucleic acid sequence, characterized in that it comprises an enzymatic system having RNAse H activity and at least one chimeric primer comprising, successively in the direction 5 '→ 3': - un segment, facultatif, de type ADN,a segment, optional, of the DNA type, - un segment de type ARN,a segment of the RNA type, - et un segment de type ADN.and a segment of the DNA type. 9. Nécessaire selon la revendication 8, caractérisé par le fait qu'il contient en outre un système enzymatique ayant une activité d'ADN polymerase ADN -dépendante et une activité de déplacement de brin. 9. Kit according to claim 8, characterized in that it further contains an enzymatic system having DNA-dependent DNA polymerase activity and strand displacement activity.
PCT/FR1996/001166 1995-07-24 1996-07-24 Method for amplifying nucleic acid sequences by translocation using chimeric primers Ceased WO1997004126A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA2200627A CA2200627C (en) 1995-07-24 1996-07-24 Method for amplifying nucleic acid sequences by translocation using chimeric primers
EP96926456A EP0787209B1 (en) 1995-07-24 1996-07-24 Method for amplifying nucleic acid sequences by displacement using chimeric primers
AT96926456T ATE214741T1 (en) 1995-07-24 1996-07-24 METHOD FOR AMPLIFYING NUCLEIN SEQUENCES BY DISPLACEMENT USING CHIMERIC PRIMERS
US08/817,035 US5824517A (en) 1995-07-24 1996-07-24 Method for amplifying nucleic acid sequences by strand displacement using DNA/RNA chimeric primers
DE69619959T DE69619959T2 (en) 1995-07-24 1996-07-24 METHOD FOR AMPLIFYING NUCLEIC SEA SEQUENCES BY DISPLACEMENT WITH THE AID OF CHIMERAL PRIMERS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9508945A FR2737223B1 (en) 1995-07-24 1995-07-24 METHOD OF AMPLIFYING NUCLEIC ACID SEQUENCES BY MOVEMENT USING CHIMERIC PRIMERS
FR95/08945 1995-07-24

Publications (2)

Publication Number Publication Date
WO1997004126A1 WO1997004126A1 (en) 1997-02-06
WO1997004126B1 true WO1997004126B1 (en) 1997-02-27

Family

ID=9481300

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1996/001166 Ceased WO1997004126A1 (en) 1995-07-24 1996-07-24 Method for amplifying nucleic acid sequences by translocation using chimeric primers

Country Status (7)

Country Link
US (1) US5824517A (en)
EP (1) EP0787209B1 (en)
AT (1) ATE214741T1 (en)
CA (1) CA2200627C (en)
DE (1) DE69619959T2 (en)
FR (1) FR2737223B1 (en)
WO (1) WO1997004126A1 (en)

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