WO1997044281A1 - Biological purification of septic tanks by extender effect - Google Patents

Abstract

The invention features the preparation of a dry Bacillus spore premix with extender effect to facilitate the long term continuous treatment of domestic effluents.

Description

 DESCRIPTION

"Bio-purification of septic tanks by delay effect"

The biological preparation developed by the firm BEVIL sa allows to continuously inoculate, for a minimum period of 6 months, a sceptual pit with a mixture of spores of non-pathogenic bacteria of the genus "Bacillus" in order to speed up the processes biological degradations of biodegradable organic matter contained in household effluents

This preparation is composed of 4 elements all perfectly compatible with the requirements of biodegradability linked to use in the bioepuraϋon sector and in accordance with the directives promulgated by the European Commission under numbers 219/90 and 220/90

The first element (Compound A) is a mixture of spores produced by 4 different Bacillus species (poiymixa, megateπum, psychrophilus and pumilus) characterized by their capacity to hydrolyze biopolymers (starch, protein, lipids and cellulose) under temperature conditions between 10 and 45 ° celsius.

The second element (Compound B) is a natural polymer of manuronic and guluronic acids approved as a food additive by the FDA (Food and Drug Admirustrauon of the USA) with the particularity of forming a three-dimensional matnec in the presence of polyvalent cations (calcium for example)

The third element (Compound C) is a cellulose-laden derivative, carboxymethylcellulose (CMC) which is added as a filler in the manufacture of the material

The fourth element (Compound D) consists of a charge in / ymaϋquc (amylasc, proteasc and cellulase) which accelerates the germination of spores in the pit

This preparation is in the form of dry granules with a relative humidity of less than 7% allowing long-term storage (> 3 years)

These granules can then be incorporated into dry preparations allowing easy handling of the biological material prepared either in the form of heterogeneous powders, tablets or any other form according to commercial needs. The concentration of Bacillus spore preparations is established in order to ensure a continuous supply of 100,000 spores per 24 hours and per gram of preparation

Compound A

The following Bacillus strains (Bacillus poiymixa, Bacillus psychrophilus, Bacillus megaterium and Bacillus puminlus) are propagated in culture immersed in a growth medium comprising yeast extract, peptones, nutπent broth (Difco) and mineral salts. Sporulation is complete after 48 to 72 hours of growth, the spores are harvested by continuous centπfugation of the cultures. In a second step, the spores are suspended in a preparation allowing their drying by atomization and obtaining a powder with 10 ⁿ spores per gram.

Compound B

The sodium alginate used is marketed by the firm PROTAN under the label PROTANAL

SF- 120

Compound C

Carboxymethyl cellulose (CMC) is marketed by hrme SIGMA Chemical under the label Carboxymethyl cellulose-Sodium sali (Low viscosity)

Compound D ^•

The enzymatic activities (amylase, protease and cellulase), provided by specialized firms are adsorbed on a preparation of chaigé cellulose Compounds A, B, C and D are mixed in the liquid phase at the desired concentrations and then extruded into a solution of Calcium chloride to obtain beads with an average diameter of 2 mm. This material is then dried to a residual relative humidity of less than 7%. The dried preparation is then used as a raw material for the formulation of commercial products.

Claims

1 ° Pπmauté as a delay effect additive for a bio-purification system for septic tanks. 2 ° Simple application of the dry product in various formulations. 3 ° All the components of the product are perfectly biodegradable 4 ° Biological product supplied complete for formulation. 5 ° Effect claimed effective in the temperature range from 10 to 50 ° C. f? Revedicated effect of the delay effect for six months in the pits. AMENDED CLAIMS [received by the International Bureau on March 18, 1997 (March 18, 1997); claims 1-6 replaced by new claims 1-4 (1 page)] Additive with delay effect for bio-purification system of septic tanks. This additive is composed of calcium alginate granules encapsulating carboxymethylcellulose, microorganisms in non-vegetative form and certain enzymatic preparations specific to the medium to be treated. Additive with a delay effect for a bio-purification system for septic tanks according to claim 1, characterized by a biodegradability of the granules spread over a period of 6 months allowing continuous inoculation of the septic tank from a single application. The septic tank is thus permanently inoculated by microorganisms in non-vegetative form. These transform into a vegetative cell on contact with the material to be treated which contains the elements necessary for bacterial development. Additive with delay effect for a bio-purification system for septic tanks according to claim 1 with a claimed effect in the range from 10 ° C to 50 ° C. Additive with delay effect for a bio-purification system for septic tanks according to claim 1 with a claimed effect after contamination of the tank by antibiotics or chemicals thanks to the deposition of granules in the bottom of the tank where the action of these products is considered negligible, but above all by the action of autonomous continuous reinoculation of the pit until complete neutralization of the poison effect.