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WO1997041243A3 - A cytoplasmic gene expression system which utilizes a prokaryotic rna polymerase autogene - Google Patents

A cytoplasmic gene expression system which utilizes a prokaryotic rna polymerase autogene Download PDF

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Publication number
WO1997041243A3
WO1997041243A3 PCT/US1997/007030 US9707030W WO9741243A3 WO 1997041243 A3 WO1997041243 A3 WO 1997041243A3 US 9707030 W US9707030 W US 9707030W WO 9741243 A3 WO9741243 A3 WO 9741243A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna polymerase
utilizes
gene expression
expression system
prokaryotic rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/007030
Other languages
French (fr)
Other versions
WO1997041243A9 (en
WO1997041243A2 (en
Inventor
Leaf Huang
Paul Robbins
Daniel Jaffurs
Marni Brisson
Song Li
Jingping Yang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pittsburgh
Original Assignee
University of Pittsburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pittsburgh filed Critical University of Pittsburgh
Priority to EP97921403A priority Critical patent/EP0910653A2/en
Priority to AU27446/97A priority patent/AU2744697A/en
Priority to JP09539094A priority patent/JP2000510334A/en
Publication of WO1997041243A2 publication Critical patent/WO1997041243A2/en
Publication of WO1997041243A9 publication Critical patent/WO1997041243A9/en
Publication of WO1997041243A3 publication Critical patent/WO1997041243A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The present invention discloses novel prokaryotic RNA polymerase autogenes which can be used in eukaryotic cytoplasmic expression systems.
PCT/US1997/007030 1996-04-26 1997-04-25 A cytoplasmic gene expression system which utilizes a prokaryotic rna polymerase autogene Ceased WO1997041243A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP97921403A EP0910653A2 (en) 1996-04-26 1997-04-25 A cytoplasmic gene expression system which utilizes a prokaryotic rna polymerase autogene
AU27446/97A AU2744697A (en) 1996-04-26 1997-04-25 A cytoplasmic gene expression system which utilizes a prokaryotic rna polymerase autogene
JP09539094A JP2000510334A (en) 1996-04-26 1997-04-25 Cytoplasmic gene expression system using prokaryotic RNA polymerase self gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1630496P 1996-04-26 1996-04-26
US60/016,304 1996-04-26

Publications (3)

Publication Number Publication Date
WO1997041243A2 WO1997041243A2 (en) 1997-11-06
WO1997041243A9 WO1997041243A9 (en) 1998-01-22
WO1997041243A3 true WO1997041243A3 (en) 1998-02-19

Family

ID=21776448

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/007030 Ceased WO1997041243A2 (en) 1996-04-26 1997-04-25 A cytoplasmic gene expression system which utilizes a prokaryotic rna polymerase autogene

Country Status (5)

Country Link
EP (1) EP0910653A2 (en)
JP (1) JP2000510334A (en)
AU (1) AU2744697A (en)
CA (1) CA2250759A1 (en)
WO (1) WO1997041243A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4312957B2 (en) 1998-01-15 2009-08-12 キングス・カレツジ・ロンドン Ribozyme nucleic acids that cleave CCR5 or CXCR4
US6342596B1 (en) * 1998-05-05 2002-01-29 Hsf Pharmaceuticals S.A. Molecular regulatory circuits to achieve sustained activation of genes of interest by a single stress
US7364750B2 (en) 2001-04-30 2008-04-29 The University Of British Columbia Autogene nucleic acids encoding a secretable RNA polymerase
US20040142892A1 (en) * 2001-04-30 2004-07-22 The University Of British Columbia Autogene nucleic acids encoding a secretable RNA polymerase
GB0406330D0 (en) * 2004-03-20 2004-04-21 Univ Sheffield Gene screen
DK3289088T3 (en) 2015-04-30 2020-06-29 Engenes Biotech Gmbh DISCOVERING GROWTH FROM PROTEIN PRODUCTION

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0426455A2 (en) * 1989-10-31 1991-05-08 Sagami Chemical Research Center Cloning vector plasmid, vector-primer derived therefrom and preparation method of cDNA bank using the same
WO1991019809A1 (en) * 1990-06-18 1991-12-26 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Eukaryotic expression vector system
WO1994026911A1 (en) * 1993-05-14 1994-11-24 Ohio University Edison Animal Biotechnology Institute A gene expression system utilizing an rna polymerase prebound to dna
EP0667393A2 (en) * 1994-01-13 1995-08-16 Enzo Diagnostics, Inc. Novel process, construct and conjugate for producing multiple nucleic acid copies

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0426455A2 (en) * 1989-10-31 1991-05-08 Sagami Chemical Research Center Cloning vector plasmid, vector-primer derived therefrom and preparation method of cDNA bank using the same
WO1991019809A1 (en) * 1990-06-18 1991-12-26 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Eukaryotic expression vector system
WO1994026911A1 (en) * 1993-05-14 1994-11-24 Ohio University Edison Animal Biotechnology Institute A gene expression system utilizing an rna polymerase prebound to dna
EP0667393A2 (en) * 1994-01-13 1995-08-16 Enzo Diagnostics, Inc. Novel process, construct and conjugate for producing multiple nucleic acid copies

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
B. SEED: "An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor", NATURE, vol. 329, 29 October 1987 (1987-10-29), MACMILLAN JOURNALS LTD., LONDON,UK, pages 840 - 842, XP002041264 *
CHEN XIAOZHUO ET AL: "A SELF-INITIATING EUKARYOTIC TRANSIENT GENE EXPRESSION SYSTEM BASED ON COTRANSFECTION OF BACTERIOPHAGE T7 TNA POLYMERASE AND DNA VECTORS CONTAINING A T7 AUTOGENE", NUCLEIC ACIDS RESEARCH, vol. 22, no. 11, 11 June 1994 (1994-06-11), pages 2114 - 2120, XP002029322 *
ELROY-STEIN O ET AL: "CYTOPLASMIC EXPRESSION SYSTEM BASED ON CONSTITUTIVE SYNTHESIS OF BACTERIOPHAGE T7 POLYMERASE IN MAMMALIAN CELLS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 87, no. 17, 1 September 1990 (1990-09-01), pages 6743 - 6747, XP000563742 *
FUERST T R ET AL: "EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE T7 RNA POLYMERASE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 83, no. 21, 1 November 1986 (1986-11-01), pages 8122 - 8126, XP000563743 *
GAO X ET AL: "A SUSTAINED, CYTOPLASMIC TRANSGENE EXPRESSION SYSTEM DELIVERED BY CATIONIC LIPOSOMES", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 200, no. 3, 16 May 1994 (1994-05-16), pages 1201 - 1206, XP002029321 *
GAO X ET AL: "CYTOPLASMIC EXPRESSION OF A REPORTER GENE BY CO-DELIVERY OF T7 RNA POLYMERASE AND T7 PROMOTER SEQUENCE WITH CATIONIC LIPOSOMES", NUCLEIC ACIDS RESEARCH, vol. 21, no. 12, 25 June 1993 (1993-06-25), pages 2867 - 2872, XP000572618 *
LI S ET AL: "DC-CHOL LIPID SYSTEM IN GENE TRANSFER", JOURNAL OF CONTROLLED RELEASE, vol. 39, no. 2/03, 1 May 1996 (1996-05-01), pages 373 - 381, XP000589694 *

Also Published As

Publication number Publication date
JP2000510334A (en) 2000-08-15
CA2250759A1 (en) 1997-11-06
AU2744697A (en) 1997-11-19
WO1997041243A2 (en) 1997-11-06
EP0910653A2 (en) 1999-04-28

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