WO1997040165B1 - Novel human cytomegalovirus dna constructs and uses therefor - Google Patents
Novel human cytomegalovirus dna constructs and uses thereforInfo
- Publication number
- WO1997040165B1 WO1997040165B1 PCT/US1997/006866 US9706866W WO9740165B1 WO 1997040165 B1 WO1997040165 B1 WO 1997040165B1 US 9706866 W US9706866 W US 9706866W WO 9740165 B1 WO9740165 B1 WO 9740165B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- immunogenic composition
- pδrc
- group
- hcmv
- human cytomegalovirus
- Prior art date
Links
- 241000701024 Human betaherpesvirus 5 Species 0.000 title claims abstract 15
- 238000000034 method Methods 0.000 claims abstract 12
- 239000013612 plasmid Substances 0.000 claims abstract 7
- 230000028993 immune response Effects 0.000 claims abstract 4
- 108090000623 proteins and genes Proteins 0.000 claims abstract 3
- 102000004169 proteins and genes Human genes 0.000 claims abstract 3
- 230000002163 immunogen Effects 0.000 claims 20
- 108020004414 DNA Proteins 0.000 claims 9
- 102000053602 DNA Human genes 0.000 claims 6
- 239000000427 antigen Substances 0.000 claims 6
- 102000036639 antigens Human genes 0.000 claims 6
- 108091007433 antigens Proteins 0.000 claims 6
- 241001465754 Metazoa Species 0.000 claims 3
- 241000700605 Viruses Species 0.000 claims 2
- 206010011831 Cytomegalovirus infection Diseases 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 101150029683 gB gene Proteins 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 230000037452 priming Effects 0.000 claims 1
- 230000003362 replicative effect Effects 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 210000002845 virion Anatomy 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
Abstract
Novel DNA molecules for in vitro and in vivo expression of HCMV gB, gB transmembrane-deleted derivatives, pp65, pp150, and IE-exon-4 proteins are described. Preferably, the molecules are plasmids. Also described are methods of using these DNA molecules to induce immune responses to HCMV, and the use of a plasmid of the invention to prime immune responses to HCMV vaccines.
Claims
AMENDED CLAIMS
[received by the International Bureau on 25 November 1997 (25.11.97); original claims 13-28 replaced by new claims 13-32; remaining claims unchanged (4 pages)]
13. A pCBgBΔtm plasmid, said plasmid comprising the human cytomegalovirus (HCMV) gB gene containing a deletion in the transmembrane domain.
14. An immunogenic composition comprising a carrier and a DNA molecule according to any of claims 1-5.
15. The immunogenic composition according to claim 14 wherein the DNA molecule is selected from the group consisting of:
(a) pΔRC-gB;
(b) pTet-gB;
(c) pΔRC-pp65;
(d) pΔRC-gE ;
(e) pΔRC-ppl50;
(f) pCBgB;
(g) pCBgBΔtm; and (h) pΔRC-exon-4.
16. The immunogenic composition according to claim 14 or 15 comprising two or more DNA molecules.
17. The immunogenic composition according to claim 15 comprising a first DNA molecule which comprises a sequence encoding the gB antigen or a gB derivative, and a second DNA molecule which comprises a sequence encoding the pp65 antigen.
18. The immunogenic composition according to any of claims 13 to 17 wherein the carrier is selected from the group consisting of saline and isotonic water.
44
19. A method of inducing human cytomegalovirus-specific (HCMV) immune responses in an animal, comprising the step of administering to said animal an effective amount of a first immunogenic composition according to any of claims 14 to 18.
20. The method according to claim 19 wherein the composition comprises pTet-gB and pΔRC-pp65.
21. The method according to claim 19 further comprising the step of administering a second immunogenic composition to said animal, said second immunogenic composition comprising a plasmid selected from the group consisting of:
(a) pΔRC-gB;
(b) pTet-gB;
(c) pΔRC-pρ65;
(d) pΔRC-gE ;
(e) pΔRC-ppl 50;
(f) pCBgB;
(g) pCBgBΔtm; and (h) pΔRC-IE-Exon-4.
22. The method according to claim 19, wherein said second immunogenic composition is administered between about 2 to about 15 weeks following administration of said first immunogenic composition.
23. The use of a DNA molecule according to any of claims 1 to 5 or a plasmid according to any of claims 6 to 13 in the preparation of a medicament to treat a cytomegalovirus infection.
45
24. A method of priming immune responses to a selected human cytomegalovirus immunogenic composition, comprising the steps of: administering a first immunogenic composition according to any of claims 14 to 18 and administering the selected human cytomegalovirus immunogenic composition.
25. The method according to claim 24 wherein the first immunogenic composition is administered between about 4 and 15 weeks prior to administration of the selected immunogenic composition.
26. The method according to claim 24 or claim 25 wherein the first immunogenic composition comprises pTet-gB.
27. The method according to claim 26, wherein pTet-gB is administered in an amount between about 50 μg to about 160 μg.
28. The method according to claim 24, wherein the selected immunogenic composition comprises an immunogen selected from the group consisting of a recombinant virus comprising an HCMV immunogen, an HCMV protein, and HCMV virions.
29. The method according to claim 28 wherein the HCMV protein is gB.
30. The method according to claim 28 wherein the recombinant virus is selected from the group consisting of Ad5.gb and Ad5-lE-exon-4.
46
31. A DNA molecule which is non-replicating in mammals and comprises a sequence encoding a human cytomegalovirus antigen selected from the group consisting of:
(a) pp65; (b) pP150;
(c) immediate-early exon-4;
(d) gB and an antigen of (a) to (c);
(e) a gB derivative lacking at least the transmembrane domain and an antigen of (a) to (c); and
(f) a combination of antigens (a) to (c).
32. An immunogenic composition comprising a carrier and at least two DNA molecules, wherein said DNA molecules are selected from the group consisting of:
(a) gB;
(b) a gB derivative lacking at least the transmembrane domain;
(c) pp65; (d) ρpl50; and
(e) immediate-early exon-4.
STATEMENT UNDER ARTICLE 19
Following entry of the attached replacement pages, claims 1 - 32 are pending.
The claims have been amended to recite two plasmid constructs disclosed throughout the application, but not previously claimed. See, new claims 12 and 13, amended claims 15 and 21. Particularly, pCBgB and pCBgBΔtm are supported in Example 14, page 34, line 1 through page 37, line 10 of the specification as filed. No new matter is added by this amendment. Inclusion of these two claims requires addition of two additional inventors, as requested in the attached letter.
In addition, two new independent claims have been added, which are supported by original claims 1 and 12, but are of narrower scope. These claims are believed to overcome the prior art cited in the International Search Report.
48
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/171,699 US6448389B1 (en) | 1996-04-23 | 1997-04-22 | Human cytomegalovirus DNA constructs and uses therefor |
| AU28098/97A AU2809897A (en) | 1996-04-23 | 1997-04-22 | Novel human cytomegalovirus dna constructs and uses therefor |
| EP97922429A EP0914441A2 (en) | 1996-04-23 | 1997-04-22 | Novel human cytomegalovirus dna constructs and uses therefor |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1571796P | 1996-04-23 | 1996-04-23 | |
| US60/015,717 | 1996-04-23 |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US09/171,699 A-371-Of-International US6448389B1 (en) | 1996-04-23 | 1997-04-22 | Human cytomegalovirus DNA constructs and uses therefor |
| US10/223,538 Division US20030120060A1 (en) | 1996-04-23 | 2002-08-19 | Human cytomegalovirus DNA constructs and uses therefor |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO1997040165A1 WO1997040165A1 (en) | 1997-10-30 |
| WO1997040165B1 true WO1997040165B1 (en) | 1998-01-08 |
| WO1997040165A9 WO1997040165A9 (en) | 1998-02-12 |
Family
ID=21773167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1997/006866 WO1997040165A1 (en) | 1996-04-23 | 1997-04-22 | Novel human cytomegalovirus dna constructs and uses therefor |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US6448389B1 (en) |
| EP (1) | EP0914441A2 (en) |
| AU (1) | AU2809897A (en) |
| WO (1) | WO1997040165A1 (en) |
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| AU2809897A (en) | 1996-04-23 | 1997-11-12 | Wistar Institute Of Anatomy And Biology, The | Novel human cytomegalovirus dna constructs and uses therefor |
| WO1998006408A1 (en) | 1996-08-14 | 1998-02-19 | The Wistar Institute Of Anatomy And Biology | Methods and compositions for preventing or retarding the development of atherosclerotic lesions |
| US6562345B1 (en) | 1996-11-12 | 2003-05-13 | City Of Hope | Immuno-reactive peptide CTL epitopes of human cytomegalovirus |
| AU4351900A (en) * | 1999-04-15 | 2000-11-02 | Board Of Regents, The University Of Texas System | Inducible vaccines |
| EP1612217A3 (en) * | 2000-03-27 | 2006-01-18 | City of Hope | Immuno-reactive peptide CTL epitopes of human cytomegalovirus |
| US6783939B2 (en) * | 2000-07-07 | 2004-08-31 | Alphavax, Inc. | Alphavirus vectors and virosomes with modified HIV genes for use in vaccines |
| JP2004504038A (en) | 2000-07-13 | 2004-02-12 | ザ ジョンズ ホプキンス ユニバーシティー スクール オブ メディシン | Detection and treatment of polycystic kidney disease |
| AU2001293798B2 (en) * | 2000-09-09 | 2006-12-21 | Basf Plant Science Gmbh | Modified tet-inducible system for regulation of gene expression in plants |
| JP4099058B2 (en) | 2000-10-20 | 2008-06-11 | シティ・オブ・ホープ | Immunoreactive peptide CTL epitope of human cytomegalovirus PP150 |
| DE60233061D1 (en) * | 2001-09-06 | 2009-09-03 | Alphavax Inc | ALPHAVIRUS REPLICON VECTOR SYSTEMS |
| AU2003301148A1 (en) | 2002-12-23 | 2004-07-22 | Vical Incorporated | Codon-optimized polynucleotide-based vaccines against human cytomegalovirus infection |
| WO2004074439A2 (en) * | 2003-02-14 | 2004-09-02 | Biogen Idec Ma Inc. | An expression cassette and vector for transient or stable expression of exogenous molecules |
| WO2004076645A2 (en) * | 2003-02-27 | 2004-09-10 | University Of Massachusetts | Compositions and methods for cytomegalovirus treatment |
| KR101518309B1 (en) * | 2003-03-20 | 2015-05-08 | 알파벡스, 인크. | Improved alphavirus replicons and helper constructs |
| DK1651666T3 (en) * | 2003-07-11 | 2009-08-31 | Alphavax Inc | Alphavirus-based cytomegalovirus vaccines |
| WO2006004661A1 (en) * | 2004-06-25 | 2006-01-12 | Medimmune Vaccines, Inc. | Recombinant human cytomegalovirus and vaccines comprising heterologous antigens |
| ES2670813T3 (en) * | 2007-06-21 | 2018-06-01 | Alphavax, Inc. | Alphavirus Replicon Particles for Vaccination Use |
| WO2009155535A2 (en) * | 2008-06-20 | 2009-12-23 | Duke University | Compositions, methods and kits for eliciting an immune response |
| US8812387B1 (en) | 2013-03-14 | 2014-08-19 | Csidentity Corporation | System and method for identifying related credit inquiries |
| EP3052106A4 (en) | 2013-09-30 | 2017-07-19 | ModernaTX, Inc. | Polynucleotides encoding immune modulating polypeptides |
| CN106102769A (en) * | 2013-12-11 | 2016-11-09 | 促进军事医学的亨利·M·杰克逊基金会公司 | Herpes virus hominis's trimerization Glycoprotein B, the protein complexes comprising trimerization gB and the purposes as vaccine thereof |
| WO2017070613A1 (en) | 2015-10-22 | 2017-04-27 | Modernatx, Inc. | Human cytomegalovirus vaccine |
| RU2616245C2 (en) * | 2015-12-29 | 2017-04-13 | Сейфаддин Гашим Оглы Марданлы | Composition for prevention of diseases caused by cmv infection |
| US10611800B2 (en) | 2016-03-11 | 2020-04-07 | Pfizer Inc. | Human cytomegalovirus gB polypeptide |
| MA46584A (en) | 2016-10-21 | 2019-08-28 | Modernatx Inc | HUMAN CYTOMEGALOVIRUS VACCINE |
| US11629172B2 (en) | 2018-12-21 | 2023-04-18 | Pfizer Inc. | Human cytomegalovirus gB polypeptide |
| TWI871709B (en) | 2020-06-21 | 2025-02-01 | 美商輝瑞股份有限公司 | Human cytomegalovirus gb polypeptide |
| US11406703B2 (en) | 2020-08-25 | 2022-08-09 | Modernatx, Inc. | Human cytomegalovirus vaccine |
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| AU2809897A (en) | 1996-04-23 | 1997-11-12 | Wistar Institute Of Anatomy And Biology, The | Novel human cytomegalovirus dna constructs and uses therefor |
-
1997
- 1997-04-22 AU AU28098/97A patent/AU2809897A/en not_active Abandoned
- 1997-04-22 US US09/171,699 patent/US6448389B1/en not_active Expired - Lifetime
- 1997-04-22 EP EP97922429A patent/EP0914441A2/en not_active Withdrawn
- 1997-04-22 WO PCT/US1997/006866 patent/WO1997040165A1/en not_active Application Discontinuation
-
2002
- 2002-08-19 US US10/223,538 patent/US20030120060A1/en not_active Abandoned
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