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WO1996033277A3 - Lipases preduodenales recombinantes et polypeptides derives produits par les plantes, leurs procedes d'obtention et leurs utilisations - Google Patents

Lipases preduodenales recombinantes et polypeptides derives produits par les plantes, leurs procedes d'obtention et leurs utilisations Download PDF

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Publication number
WO1996033277A3
WO1996033277A3 PCT/FR1996/000606 FR9600606W WO9633277A3 WO 1996033277 A3 WO1996033277 A3 WO 1996033277A3 FR 9600606 W FR9600606 W FR 9600606W WO 9633277 A3 WO9633277 A3 WO 9633277A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
polypeptide derivatives
recombinant
obtaining
processes
Prior art date
Application number
PCT/FR1996/000606
Other languages
English (en)
Other versions
WO1996033277A2 (fr
Inventor
Philippe Lenee
Veronique Gruber
Sylvie Baudino
Bertrand Merot
Claude Benicourt
Claire Cudrey
Original Assignee
Biocem S A
Inst Rech Joveinal
Philippe Lenee
Veronique Gruber
Sylvie Baudino
Bertrand Merot
Claude Benicourt
Claire Cudrey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AT96915066T priority Critical patent/ATE313632T1/de
Priority to JP53152996A priority patent/JP4077875B2/ja
Priority to AU56967/96A priority patent/AU718905B2/en
Priority to SK1401-97A priority patent/SK140197A3/sk
Priority to EP96915066A priority patent/EP0822988B1/fr
Priority to DE69635611T priority patent/DE69635611T2/de
Priority to NZ307579A priority patent/NZ307579A/xx
Priority to UA97115538A priority patent/UA73267C2/uk
Priority to BR9608011A priority patent/BR9608011A/pt
Priority to PL96322874A priority patent/PL186586B1/pl
Application filed by Biocem S A, Inst Rech Joveinal, Philippe Lenee, Veronique Gruber, Sylvie Baudino, Bertrand Merot, Claude Benicourt, Claire Cudrey filed Critical Biocem S A
Publication of WO1996033277A2 publication Critical patent/WO1996033277A2/fr
Publication of WO1996033277A3 publication Critical patent/WO1996033277A3/fr
Priority to BG101959A priority patent/BG64320B1/bg

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6454—Glycerides by esterification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Public Health (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

L'invention a pour objet l'utilisation de séquences nucléotidiques recombinantes contenant l'ADNc codant pour une lipase préduodénale, ou toute séquence dérivée de cet ADNc, pour la transformation de cellules végétales en vue de l'obtention de lipase préduodénale recombinante, ou de polypeptides dérivés. L'invention concerne également l'utilisation des plantes, ou parties de plantes, transformées génétiquement, ou d'extraits de ces végétaux, ou de lipase préduodénale recombinante ou polypeptides dérivés obtenus, dans le domaine de l'alimentation, ou pour l'obtention de médicaments, ou encore dans le domaine industriel.
PCT/FR1996/000606 1995-04-20 1996-04-19 Lipases preduodenales recombinantes et polypeptides derives produits par les plantes, leurs procedes d'obtention et leurs utilisations WO1996033277A2 (fr)

Priority Applications (11)

Application Number Priority Date Filing Date Title
BR9608011A BR9608011A (pt) 1995-04-20 1996-04-19 Utilização de uma sequéncia de nucleotédeos recombinante e de plantas ou partes de plantas sequéncia de nucleotídeos recombinante vetor notadamente plasmídeo hospedeiro celular processos de obtenção de lgc recombinante e de bio- carburante e de bioconvers o enzimática ou de bio-catálise plantas ou partes de plantas lgc e lgc recombinante enzimaticamente ativo extrato vegetal com atividade enzimática composição farmacéutica alimentos funcionais preparações enzimáticas ésteres de ácidos graxos vegestais e biocarburante
AU56967/96A AU718905B2 (en) 1995-04-20 1996-04-19 Recombinant preduodenal lipases and polypeptides derivatives produced by plants, processes for obtaining them and their uses
SK1401-97A SK140197A3 (en) 1995-04-20 1996-04-19 Recombinant preduodenal lipases and polypeptide derivatives produced by plants, processes for obtaining them and their uses
EP96915066A EP0822988B1 (fr) 1995-04-20 1996-04-19 Lipases preduodenales recombinantes et polypeptides derives produits par les plantes, leurs procedes d'obtention et leurs utilisations
DE69635611T DE69635611T2 (de) 1995-04-20 1996-04-19 Durch pflanzen hergestellte rekombinante preduodenale lipase und abgeleitete polypeptide, verfahren zur herstellung und ihre verwendung
AT96915066T ATE313632T1 (de) 1995-04-20 1996-04-19 Durch pflanzen hergestellte rekombinante preduodenale lipase und abgeleitete polypeptide, verfahren zur herstellung und ihre verwendung
UA97115538A UA73267C2 (en) 1995-04-20 1996-04-19 Recombinant gastric lipases of dog (lgc) and human (lgh), polypeptide derivatives thereof, method for producing by plants tranformation and use thereof
NZ307579A NZ307579A (en) 1995-04-20 1996-04-19 Recombinant preduodenal lipases and polypeptides derivatives produced by plants, processes for obtaining them and their uses
PL96322874A PL186586B1 (pl) 1995-04-20 1996-04-19 Rekombinacyjna sekwencja nukleotydowa, wektor, gospodarz komórkowy, zastosowanie rekombinacyjnej sekwencji nukleotydowej, sposób otrzymywania aktywnego enzymu rekombinacyjnej lipazy preduodenalnej ssaków i jej pochodnej, modyfikowany materiał roślinny i jego zastosowanie, LGC rekombinacyjny aktywnyenzymatycznie, LGH rekombinacyjny aktywny enzymatycznie, ekstrakt roślinny o aktywności enzymatycznej, kompozycja farmaceutyczna, funkcjonalny środekspożywczy oraz preparat enzymatyczny
JP53152996A JP4077875B2 (ja) 1995-04-20 1996-04-19 植物によって生産される組換え前十二指腸リパーゼおよびペプチド誘導体、それらの取得方法およびそれらの用途
BG101959A BG64320B1 (bg) 1995-04-20 1997-10-10 Рекомбинантни предуоденални липази и полипептидни производни, продуцирани от растения, метод за получаването и използването им

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR95/04754 1995-04-20
FR9504754A FR2733249B1 (fr) 1995-04-20 1995-04-20 Lipase gastrique de chien recombinante et polypeptides derives produits par les plantes, leurs procedes d'obtention et leurs utilisations

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/348,930 Continuation US6573431B1 (en) 1995-03-20 1999-07-02 Recombinant preduodenal lipases and polypeptides derivatives produced by plants, processes for obtaining them and their uses

Publications (2)

Publication Number Publication Date
WO1996033277A2 WO1996033277A2 (fr) 1996-10-24
WO1996033277A3 true WO1996033277A3 (fr) 1996-11-28

Family

ID=9478306

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1996/000606 WO1996033277A2 (fr) 1995-04-20 1996-04-19 Lipases preduodenales recombinantes et polypeptides derives produits par les plantes, leurs procedes d'obtention et leurs utilisations

Country Status (26)

Country Link
US (2) US6573431B1 (fr)
EP (1) EP0822988B1 (fr)
JP (2) JP4077875B2 (fr)
KR (1) KR19990007901A (fr)
CN (1) CN1185178A (fr)
AR (1) AR004482A1 (fr)
AT (1) ATE313632T1 (fr)
AU (1) AU718905B2 (fr)
BG (1) BG64320B1 (fr)
BR (1) BR9608011A (fr)
CA (1) CA2218418A1 (fr)
CZ (1) CZ330997A3 (fr)
DE (1) DE69635611T2 (fr)
ES (1) ES2256858T3 (fr)
FR (1) FR2733249B1 (fr)
HU (1) HUP9801438A3 (fr)
IL (1) IL117955A0 (fr)
MX (1) MX9707973A (fr)
NZ (1) NZ307579A (fr)
PL (1) PL186586B1 (fr)
RU (1) RU2235129C2 (fr)
SK (1) SK140197A3 (fr)
TR (1) TR199701207T1 (fr)
UA (1) UA73267C2 (fr)
WO (1) WO1996033277A2 (fr)
ZA (1) ZA963146B (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2759857B1 (fr) * 1997-02-27 1999-04-30 Biocem Nouvelles utilisations de la sterilite male chez les plantes
FR2774379B1 (fr) * 1998-01-30 2002-03-29 Groupe Limagrain Holding Procede de production, par des cellules vegetales, d'alpha 1-antitrypsine et de ses variantes, et produits contenant l'alpha-antitrypsine ainsi obtenue
FR2777155B1 (fr) 1998-04-09 2000-06-23 Meristem Therapeutics Semences et plantes dont le pouvoir germinatif est detruit par l'absence totale ou partielle d'au moins une reserve energetique indispensable a la germination
FR2810667B1 (fr) * 2000-06-23 2004-09-03 Warner Lambert Co Procede d'isolement et de purification d'une proteine, et proteine obtenue
US7288124B2 (en) 2004-09-08 2007-10-30 L'oreal S.A. Heteroaromatic binuclear black direct dyes
EP2484768A3 (fr) 2005-07-18 2012-11-21 Protalix Ltd. Administration mucosale ou enterale de macromolecules biologiquement actives
FR3036119B1 (fr) * 2015-05-15 2019-04-19 Universite De Picardie Jules Verne Procede de production de proteines d'interet a partir d'une structure vegetale
EP4501403A3 (fr) * 2019-08-30 2025-05-14 Société des Produits Nestlé S.A. Variantes de lipase modifiées
CN112480233B (zh) * 2020-12-14 2022-05-27 上海交通大学 一种生物活性肽iahpklgkrir及其制备方法和应用
CN115669542B (zh) * 2022-11-04 2023-08-25 西北农林科技大学 一种植物组织培养脱毒方法

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2090613A (en) * 1980-12-30 1982-07-14 Inst Francais Du Petrole Combustible compositions comprising alcohols and fatty acid esters useful in particular as diesel fuels
WO1986001532A1 (fr) * 1984-08-21 1986-03-13 Celltech Limited Polypeptide et composition a base de polypeptide
GB2188057A (en) * 1986-02-04 1987-09-23 Inst Penyelidikan Minyak Kelap Transesterification of fats and oils
EP0261016A1 (fr) * 1986-09-17 1988-03-23 Jouveinal S.A. Lipases et extraits lipasiques, leur procédé de préparation et leur application en thérapeutique
WO1991006661A1 (fr) * 1989-11-03 1991-05-16 Opta Food Ingredients, Inc. Production in situ de monoglycerides et de diglycerides catalysee par lipase
EP0449376A2 (fr) * 1990-03-23 1991-10-02 Gist-Brocades N.V. Production d'enzymes dans des semences et leur utilisation
WO1992001042A1 (fr) * 1990-07-06 1992-01-23 Novo Nordisk A/S Plantes transgeniques exprimant des enzymes industrielles
WO1993003161A1 (fr) * 1990-10-22 1993-02-18 Biosource Genetics Corporation Acides nucleiques viraux recombines de plantes
EP0542629A1 (fr) * 1991-11-13 1993-05-19 Institut De Recherche Jouveinal (I.R.J) Acides nucléiques codant pour la lipase gastrique de lapin et dérivés polypeptidiques, leur utilisation pour la production de ces polypeptides, et compositions pharmaceutiques à base de ces derniers
WO1994013816A1 (fr) * 1992-12-16 1994-06-23 Institut De Recherche Jouveinal Lipase gastrique du chien recombinante et compositions pharmaceutiques
WO1994020610A1 (fr) * 1993-03-01 1994-09-15 Astra Aktiebolag Variantes de la lipase stimulee par les sels biliaires, molecules d'adn les codant et mammiferes transgeniques
WO1996003511A2 (fr) * 1994-07-25 1996-02-08 Institut National Polytechnique De Toulouse (I.N.P.T.) Procede de production d'acides gras ou derives a partir de plantes oleagineuses

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US5538868A (en) * 1984-02-08 1996-07-23 Cetus Oncology Corporation Recombinant ricin toxin fragments

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2090613A (en) * 1980-12-30 1982-07-14 Inst Francais Du Petrole Combustible compositions comprising alcohols and fatty acid esters useful in particular as diesel fuels
WO1986001532A1 (fr) * 1984-08-21 1986-03-13 Celltech Limited Polypeptide et composition a base de polypeptide
GB2188057A (en) * 1986-02-04 1987-09-23 Inst Penyelidikan Minyak Kelap Transesterification of fats and oils
EP0261016A1 (fr) * 1986-09-17 1988-03-23 Jouveinal S.A. Lipases et extraits lipasiques, leur procédé de préparation et leur application en thérapeutique
WO1991006661A1 (fr) * 1989-11-03 1991-05-16 Opta Food Ingredients, Inc. Production in situ de monoglycerides et de diglycerides catalysee par lipase
EP0449376A2 (fr) * 1990-03-23 1991-10-02 Gist-Brocades N.V. Production d'enzymes dans des semences et leur utilisation
WO1992001042A1 (fr) * 1990-07-06 1992-01-23 Novo Nordisk A/S Plantes transgeniques exprimant des enzymes industrielles
WO1993003161A1 (fr) * 1990-10-22 1993-02-18 Biosource Genetics Corporation Acides nucleiques viraux recombines de plantes
EP0542629A1 (fr) * 1991-11-13 1993-05-19 Institut De Recherche Jouveinal (I.R.J) Acides nucléiques codant pour la lipase gastrique de lapin et dérivés polypeptidiques, leur utilisation pour la production de ces polypeptides, et compositions pharmaceutiques à base de ces derniers
WO1994013816A1 (fr) * 1992-12-16 1994-06-23 Institut De Recherche Jouveinal Lipase gastrique du chien recombinante et compositions pharmaceutiques
WO1994020610A1 (fr) * 1993-03-01 1994-09-15 Astra Aktiebolag Variantes de la lipase stimulee par les sels biliaires, molecules d'adn les codant et mammiferes transgeniques
WO1996003511A2 (fr) * 1994-07-25 1996-02-08 Institut National Polytechnique De Toulouse (I.N.P.T.) Procede de production d'acides gras ou derives a partir de plantes oleagineuses

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BENICOURT C ET AL: "Potential use of a recombinant dog gastric lipase as an enzymatic supplement to pancreatic extracts in cystic fibrosis.", ESCOBAR, H., F. BAQUERO AND L. SUAREZ (ED.). INTERNATIONAL CONGRESS SERIES, NO. 1034. CLINICAL ECOLOGY OF CYSTIC FIBROSIS, 1993, 18TH EUROPEAN CYSTIC FIBROSIS CONFERENCE, MADRID, SPAIN, MAY 21-26, 1993.ELSEVIER SCIENCE PUBLISHERS B.V.: AMSTERDAM, NETHERLANDS; NEW YORK,NEW YORK, USA.ISBN: 0-444-81670-4, pages 291 - 295, XP000603098 *
PEN, J., ET AL.: "Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction", BIOTECHNOLOGY, vol. 10, pages 292 - 296, XP002013284 *

Also Published As

Publication number Publication date
CN1185178A (zh) 1998-06-17
MX9707973A (es) 1998-08-30
NZ307579A (en) 1999-11-29
BR9608011A (pt) 1999-01-05
WO1996033277A2 (fr) 1996-10-24
DE69635611T2 (de) 2006-09-14
AR004482A1 (es) 1998-12-16
AU718905B2 (en) 2000-04-20
BG64320B1 (bg) 2004-09-30
IL117955A0 (en) 1996-08-04
EP0822988A2 (fr) 1998-02-11
BG101959A (bg) 1998-07-31
HUP9801438A2 (hu) 1998-10-28
JP2007325590A (ja) 2007-12-20
DE69635611D1 (de) 2006-01-26
HUP9801438A3 (en) 2000-11-28
PL186586B1 (pl) 2004-01-30
ATE313632T1 (de) 2006-01-15
RU2235129C2 (ru) 2004-08-27
ES2256858T3 (es) 2006-07-16
JP4077875B2 (ja) 2008-04-23
FR2733249B1 (fr) 1997-06-06
EP0822988B1 (fr) 2005-12-21
UA73267C2 (en) 2005-07-15
KR19990007901A (ko) 1999-01-25
JPH11504207A (ja) 1999-04-20
PL322874A1 (en) 1998-03-02
CZ330997A3 (cs) 1998-03-18
FR2733249A1 (fr) 1996-10-25
TR199701207T1 (xx) 1998-02-21
CA2218418A1 (fr) 1996-10-24
SK140197A3 (en) 1998-07-08
US6573431B1 (en) 2003-06-03
ZA963146B (en) 1997-01-22
AU5696796A (en) 1996-11-07
US20040072317A1 (en) 2004-04-15

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